scholarly journals Cytokine changes in the aqueous humor in rubella-related Fuchs heterochromic iridocyclitis

2021 ◽  
Author(s):  
Shangkun ou ◽  
Yi Mao ◽  
Sijie Lin ◽  
Chengfang Zhu ◽  
Xiaodong Liu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Aqueous Humor ◽  
Rubella Virus ◽  
Negative Group ◽  
Virus Infections ◽  
Related Factors ◽  
Adhesion Protein ◽  
Positive Group ◽  
Significant Difference ◽  
Simplex Virus

Abstract This retrospective study aimed to determine the correlation between cytokine levels and virus status in the aqueous humor of 38 patients with Fuchs heterochromic iridocyclitis (FHI) with/without viral presence between May 2017 and January 2020. The levels of cytokines were analyzed in the groups with and without virus-related FHI. Among the patients, 50% had rubella virus, 5.26% had cytomegalovirus, and 2.63% had herpes simplex virus infections. The expression of interleukin-6 (IL-6) and IL-8 was significantly higher and that of basic fibroblast growth factor (bFGF) was significantly lower in the virus-positive group than in the virus-negative group (P = 0.015, P = 0.001, and P = 0.001, respectively). Although there was no significant difference in the mean expression of vascular cell adhesion protein 1 (VCAM-1), IL-10, and vascular endothelial growth factor (VEGF), those of VCAM-1 and IL-10 were higher (M = 1338, M = 1390, respectively; M = 6.225 and 10.600, respectively) and that of VEGF was lower (M = 134.5, M = 38.70, respectively) in the virus-positive group than in the virus-negative group. Similar findings were observed for the expressions of IL-6, IL-8, and bFGF in the rubella-positive and -negative groups. Viral presence was highly related to FHI, especially that of the rubella virus. High levels of inflammatory cytokines and low levels of neovascularization-related factors are involved in rubella-related FHI. These study findings could be helpful in the diagnosis and treatment of FHI.

Linkage between H. pylori Infection and TNF-α polymorphism in The Pregnant Women

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Ghaidaa Raheem Lateef ◽  
Azhar Omaran Al-Thahab
Keyword(s):  
Pregnant Women ◽  
Serum Antibody ◽  
Rapid Test ◽  
Negative Group ◽  
Positive Group ◽  
Gynecology And Obstetrics ◽  
Tnf Α ◽  
Significant Difference ◽  
Pcr Products ◽  
H Pylori

A study was performed on 100 pregnant women in the outpatient department of gynecology and obstetrics of Maternity and Children Hospital in Al-Diwaniya City during the period between (March to September 2016). One hundred blood samples (50 for patients and 50 for control) were collected under the supervision of the treating gynecologist. The detection of Helicobacter. pylori was done by the use of the serum antibody Rapid test. The results showed that 50 (100%) were positive and 50 (100%) were negative for H. pylori in above method.All blood of patients and control samples were used for the extraction of genomic DNA,where the 107 bp PCR product size. Genotyping of the TNF-α-308 SNP (G/A)was performed by restriction fragment length polymorphism PCR (RFLP-PCR). PCR products were digested with restr NcoI iction enzyme. Individuals with the TNF-α-308(GG) homozygote produced digested DNA bands at 80,and 20 bp bp. A heterozygous genotype ofTNF-α-308 (GA)produced 107 bp,80 bp,and 20 bp bands. Individuals with the TNF-α-308 (AA) homozygote genotype had no amplicon digested and generated only one band of 107 bp. There was a significant difference in the frequency of the TNF-α-308(GG)genotype between H. pylori positive group and H. pylori negative group(72%,78% respectively). Also for GA genotype,there was a significant difference between H. pylori positive group and H. pylori negative group(24%,18% respectively). Concerning the frequency of the TNF-α-308 (AA)genotype between H. pylori positive group and H. pylori negative group,there was no significant difference between the two groups.

scholarly journals Comparison of intravenous sedation using midazolam during dental treatment in elderly patients with/without dementia: a prospective, controlled clinical trial

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuichi Tatsuno ◽  
Yoshinari Morimoto ◽  
Megumi Hayashi ◽  
Takatoshi Iida
Keyword(s):  
Regression Analysis ◽  
Cognitive Impairment ◽  
Elderly Patients ◽  
Multiple Regression Analysis ◽  
Multiple Regression ◽  
Intravenous Sedation ◽  
Negative Group ◽  
Severe Dementia ◽  
Positive Group ◽  
Significant Difference

AbstractThe effects of intravenous sedation with midazolam on the cerebral function of elderly patients with severe dementia are unclear. This study aimed to evaluate its effects on parameters such as brainwaves and cerebral blood flow (CBF) and compare them between elderly individuals with dementia and without cognitive impairment. Ten patients with severe dementia and 10 without cognitive impairment were registered. The bispectral index (BIS) and normalized tissue hemoglobin index (nTHI), which reflects CBF using near-infrared spectroscopy, were measured. Midazolam was administered until a Modified Observer’s Assessment of Alertness/Sedation score of 2 was reached. The chi-squared, Mann–Whitney U, Wilcoxon signed-rank, and Friedman tests and multiple regression analysis were used for comparisons. Whereas a similar decline in BIS values was observed in both groups after midazolam administration (P < 0.018), there was a significant decrease by 9% in the nTHI of the dementia-positive group (P < 0.013). However, there was no significant difference in the nTHI between the dementia-positive and dementia-negative group according to the multiple regression analysis (P = 0.058). In the dementia-negative group, none of the measured values differed from the baseline values. In the dementia-positive group, sedation with midazolam resulted in a 9% decrease in the CBF.

scholarly journals Significance of nucleic acid positive anal swab in COVID-19 patients

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 332-337
Author(s):  
Xiaoli Li ◽  
Lei Rong ◽  
Peiyan Zhang ◽  
Jian Xu ◽  
Yan Rong
Keyword(s):  
Platelet Count ◽  
Clinical Symptoms ◽  
Influence Factors ◽  
Recovery Phase ◽  
Negative Group ◽  
Pharyngeal Swab ◽  
Positive Group ◽  
Oral Transmission ◽  
Significant Difference ◽  
Anal Swab

Abstract Aim We compared the clinical characteristics of patients with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) positive and negative anal swabs during coronavirus disease 2019 (COVID-19) recovery and investigated the clinical significance and influence factors of anal swab detection. Methods This study retrospectively analyzed 23 moderate COVID-19 patients in the recovery phase. They were divided into anal swab positive group (n = 13) (negative for pharyngeal swabs but positive for anal swabs) and anal swab negative group (n = 10) (negative for pharyngeal and anal swabs). The epidemiology, clinical symptoms, time of pharyngeal swabs turning negative, and laboratory results were compared. Results The time of pharyngeal swabs turning negative in the anal swab positive group was 6 (5–8.5) days, significantly longer than that in the anal swab negative group (1 (1–4.25) days), P = 0.0002). The platelet count of the anal swab positive group was significantly lower than that of the anal swab negative group (198 (135–235) × 109/L vs 240.5 (227–264.75) × 109/L, P = 0.0248). No significant difference was observed between the two groups in other variables. Conclusions The time of pharyngeal swab turning negative in anal swab positive patients is longer than that in anal swab negative patients. The platelet count can be used as an indicator for viral infection evaluation. For patients with a longer time of pharyngeal swabs turning negative, the combined testing of the anal swab and platelet counts may help to avoid pharyngeal swab false negatives, premature discharge, and the possibility of fecal-oral transmission.

Abstract 2753: Initial and 24h After Admisson DWI Can Predict Recurrence of Ischemic Event of TIA Patients

Stroke ◽  
2012 ◽  
Vol 43 (suppl_1) ◽  
Author(s):  
Yasuyuki Iguchi ◽  
Kazumi Kimura ◽  
Jyunya Aoki ◽  
Kazuto Kobayashi ◽  
Kenichiro Sakai ◽  
...  
Keyword(s):  
Recurrence Rate ◽  
Negative Group ◽  
Initial Examination ◽  
Ischemic Lesion ◽  
Ischemic Event ◽  
Positive Group ◽  
Significant Difference ◽  
Weighted Magnetic Resonance Imaging ◽  
Ischemic Attack

Background and Purpose Our aim is to investigate the diagnostic utility of diffusion weighted magnetic resonance imaging (DWI) for transient ischemic attack (TIA), not only admission but also 24 h after first study. Methods We prospectively enrolled TIA patients who were admitted within 24 h of onset. We examined trans-thoracic echocardiography, 24 h Holter electrocardiography, and carotid duplex ultrasonography in order to give the best medication. All of patients received DWI immediately after admission. At first, when we observed hyper-intense lesion considered as ischemia, additional DWI examination was not conducted. When there was no hyper-intense lesion on initial DWI study, we conducted follow-up DWI 24 h after initial examination. We defined a recurrence of ischemic event as an occurrence of symptomatic ischemic stroke or TIA at 3 months after onset. Regarding initial DWI study, we compared proportional frequency of recurrence between patients with ischemic lesion (i-positive group) and without any lesion (i-negative group), and also compared recurrence rate between patients with ischemic lesion on initial and/or follow-up studies (if-positive group) and without any lesion on both studies (if-negative group). Results We registered 100 TIA patients (men; 63, median age; 74 years, ABCD2 score <3; 32). On initial DWI study, 34 patients had ischemic lesion (i-positive group) and 66 had no lesion (i-negative group). Among 66 patients of i-negative group, 12 (19%) had hyper-intense lesion in follow-up study. Finally, there were 46 patients in if-positive group and 54 in if- negative. Recurrence ischemic event occurred in 19 (19%) of 100 patients. There was no significant difference of recurrence rate between i-positive and i-negative groups (27% vs. 16%, p=0.172). However, recurrence rate was significantly higher in if-positive group than these of if-negative group (29% vs. 12%, p=0.024) Conclusions New ischemic lesions on follow up DWI appeared in approximately 20% of TIA patients who did not have ischemic lesion on initial DWI. Only initial DWI but initial and follow-up DWI was important role of predicting the recurrence of ischemic events in TIA patients.

scholarly journals Relationship between renal tissues phospholipase A2 receptor and its serum antibody and clinical condition and prognosis of idiopathic membranous nephropathy: a meta-analysis

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Dan Dong ◽  
Ting-ting Fan ◽  
Ying-ying Wang ◽  
Lu Zhang ◽  
Li Song ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Serum Albumin ◽  
Membranous Nephropathy ◽  
Remission Rate ◽  
Meta Analysis ◽  
Negative Group ◽  
Urine Protein ◽  
Positive Group ◽  
Phospholipase A2 Receptor ◽  
Significant Difference

Abstract Objective To investigate the correlation of M-type phospholipase A2 receptor (PLA2R) expression and serum anti-PLA2R antibody with the clinical parameters and prognosis of patients with idiopathic membranous nephropathy (IMN). Methods A literature search for relevant original articles published between January 2009 and October 2019 was conducted on domestic and foreign databases. RevMan 5.3 software was used for meta-analysis. Results Eighteen studies were included in this meta-analysis. There were 1235 anti-PLA2R antibody-positive and PLA2R-positive patients, and 407 serum anti-PLA2R antibody-negative and PLA2R-negative patients. Compared with negative group, patients in the serum PLA2R antibody -positive group had lower serum albumin [SMD = -1.11, 95% CI (− 1.82, − 0.40), P < 0.00001], higher age [MD = 2.71, 95% CI (1.94, 3.48), P < 0.00001], and lower estimated glomerular filtration rate (eGFR) [MD = -10.34, 95% CI (− 12.09, − 8.60), P < 0.00001]; no significant between-group difference was observed with respect to 24-h urine protein and serum creatinine. However, no significant difference was observed between renal tissues PLA2R -positive and -negative groups with respect to serum albumin, eGFR, serum creatinine, and 24-h urine protein. Remission rate in the serum anti-PLA2R antibody -positive group was lower than that in the -negative group [OR = 0.41, 95% CI (0.28, 0.61),P < 0.00001]; however, no significant between-group difference in this respect was observed between the renal tissue PLA2R-positive and -negative groups. In the serum anti-PLA2R antibody -positive group, the higher titer subgroup had lower remission rate [OR = 0.19, 95% CI (0.07, 0.55),P = 0.002]. No significant difference was observed between anti-PLA2R antibody -positive and -negative groups with respect to adverse events. Serum anti-PLA2R antibody titer did not affect the adverse event rate. Conclusion As compared to PLA2R, serum anti-PLA2R antibody is more closely related with IMN disease progression.

scholarly journals Correlation of Clinicopathological Features and IL6 Expression in Tumor Budding of Colon Adenocarcinoma

2020 ◽  
Author(s):  
Koichi Sato ◽  
Takeshi Uehara ◽  
Mai Iwaya ◽  
Tomoyuki Nakajima ◽  
Yosuke Tobe ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Progression ◽  
Prognostic Significance ◽  
Colon Adenocarcinoma ◽  
Tumor Budding ◽  
Rank Test ◽  
Negative Group ◽  
Positive Group ◽  
Significant Difference ◽  
Cox Proportional Hazard Regression

Abstract Background: Interleukin-6 (IL6) is one of the main cytokines produced by cancer-associated fibroblasts (CAFs). IL6 is linked with cancer progression and poor prognosis by activating cancer cells and modifying the cancer microenvironment. However, little is known about the expression of IL6 in tumor budding (TB) and its association with TB in colon adenocarcinoma (CA). Methods: The clinicopathological and prognostic significance of IL6 in TB was examined using a tissue microarray consisting of 36 patient samples of TB in CA. IL6 mRNA was detected by RNAscope kit. Patients were stratified into negative and positive IL6 expression groups.Results: IL6 expression was overwhelmingly observed in CAFs but was negligible in cancer cells. In the IL6-positive group in CAFs, TB grade was higher than in the IL6-negative group (P=0.0161). There was a significant difference in overall survival (OS) between CA cases in the IL6-positive group and the IL6-negative group (log rank test, P=0.0367). Cox proportional hazard regression model revealed that the IL6-negative group (OR = 0.25; 95% CI: 0.05–0.96; P=0.0440) had better OS for CA than the IL6-positive group. Conclusions: TB may be affected by IL6 expression, and IL6 expression in CAFs at TB may make IL6 an important prognostic marker.

scholarly journals The Effectiveness Fig Leaves (FICUS CARICA L) Steeping Water Towards SGOT SGPT Serum on Male Wistar Rats in Critical Hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 826-835
Author(s):  
Yosi Julianti ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Ficus Carica ◽  
Negative Group ◽  
Positive Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Food And Drink ◽  
One Way Anova

Critical Hepatitisis is the inflammation of the liver that is caused by viruses, alchol and drugs which are characterized by the increasing values of SGOT SGPT. The purpose of this study is to determine the effectiveness of fig leaves (Ficuss carica L) steeping water towards the decrease of SGOT SGPT serum on male wistar rats in critical hepatitis model. The object of this study is 30 male wistar rats with the age of 2-3 months and weight 180-200 grams and randomly divide into 3 groups, which are the negative group that is only by giving an usual food and drink, the positive group and the treatment groups are given paracetamol 120mg/ oral for 7 days. The treatment group is given 0.65 gram/ day of fig leaves steeping water for 7 days. One way ANOVA of SPSS version 24 is used to analyze this research. The result of the study shows that there is a significant difference in SGOT serum level between the treatment group, positive group and negative group (p<0,05). And there is a significant difference  in SGPT level between the treatment group and the positive group (p<0,05). The conclusion of this study is tin leaves (Ficus carica L) steeping water has an effect towards the decrease of SGOT SGPT in male Wistar rats in critical hepatitis model.

scholarly journals LGR5 Expression is Associated with Prognosis in Poorly Differentiated Gastric Adenocarcinoma

2020 ◽  
Author(s):  
Takehito Ehara ◽  
Takeshi Uehara ◽  
Tomoyuki Nakajima ◽  
Yasuhiro Kinugawa ◽  
Shota Kobayashi ◽  
...  
Keyword(s):  
Gastric Adenocarcinoma ◽  
Target Therapy ◽  
Stem Cell Marker ◽  
Rank Test ◽  
Negative Group ◽  
Positive Group ◽  
Significant Difference ◽  
Lgr5 Expression ◽  
Poorly Differentiated

Abstract Background Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is an important cancer stem cell marker in gastric cancer. However, no detailed studies are available on LGR5 expression in poorly differentiated gastric adenocarcinoma (PD-AC). Therefore, we investigated the relationship between LGR5 expression and clinicopathological data in PD-AC.Methods LGR5 expression was identified in 41 PD-AC cases using RNAscope, which is a highly sensitive RNA in situ hybridization method. Epstein–Barr virus (EBV) infection was also detected by EBV in situ hybridization.Results In PD-AC, LGR5 expression was identified in 38 of 41 cases, and 17 cases were identified as LGR5 positive. The frequency of EBV positivity tended to be higher in the LGR5-negative group than in the LGR5-positive group (P = 0.0764). Furthermore, the frequency of vascular invasion tended to be higher in the LGR5-positive group than in the LGR5-negative group (P = 0.0764). A significant difference was found in overall survival (OS) between PD-AC cases in the LGR5-positive group and LGR5-negative group (log-rank test, P = 0.0108). The Cox proportional hazard regression model revealed that the LGR5-negative group (HR = 0.29; 95% CI: 0.11–0.74; P = 0.01) showed independently better OS for PD-AC.Conclusions The correlation between LGR5 positivity and poor prognosis in PD-AC may be applicable to target therapy for LGR5 and prognostic markers. Further study is warranted.

scholarly journals Changing Trends of Color of Different Laryngeal Regions in Laryngopharyngeal Reflux Disease

2019 ◽  
Vol 99 (8) ◽  
pp. 543-547
Author(s):  
Chen Du ◽  
Paige Thayer ◽  
Yan Yan ◽  
Qingsong Liu ◽  
Li Wang ◽  
...  
Keyword(s):  
Laryngopharyngeal Reflux ◽  
Ph Monitoring ◽  
Vocal Folds ◽  
Control Group ◽  
Negative Group ◽  
Positive Group ◽  
Changing Trends ◽  
Significant Difference ◽  
False Vocal ◽  
And Control

Introduction: In our previous study, we demonstrated that there might be correlations between laryngopharyngeal reflux disorder and the hue value of laryngoscopic images. And we found that different regions of larynx have different hue values. It was hypothesized that the degree of inflammation varies between different laryngeal regions, due to an acid reflux pattern. Objective: The objective of this study was to compare the changing trends of hue values of different laryngeal regions in patients with laryngopharyngeal reflux (LPR) disease. Methods: Ninety-seven patients, including 20 pH-positive, 19 pH-negative were tested for LPR through multichannel intraluminal impedance 24-hour pH monitoring, and 58 controls with reflux symptom index less than 13. Laryngoscopic images of all patients were obtained. The hue values of 7 areas of interest, including both sides of the true vocal folds, the false vocal folds, the arytenoids, and the interarytenoid space, were quantified using a hue calculation. The analysis of variance analysis was applied to find if there was significant difference between different groups within each region. Results: (1) In the regions of both sides of the true vocal folds and interarytenoid, there was no significant difference between positive group and negative group; (2) in the regions of both sides of false vocal folds, there was no significant difference between negative group and control group; (3) in the regions of both sides of arytenoids, there was neither significant difference between positive group and negative group nor between negative group and control group; (4) in other comparisons, there were significant differences. Conclusion: Hue values of separate laryngeal regions are different. In negative group, the hue values of interarytenoid region are similar with positive group, and the sensitivity to the acid are different from different regions. The sensitivity in the true vocal folds may be present.

scholarly journals The Distribution of Vascular Endothelial Growth Factor (VEGF), Human Beta-Defensin-2 (HBD-2), and Hepatocyte Growth Factor (HGF) in Intra-Abdominal Adhesions in Children under One Year of Age

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Anna Junga ◽  
Māra Pilmane ◽  
Zane Ābola ◽  
Olafs Volrāts
Keyword(s):  
Growth Factor ◽  
Control Group ◽  
Relative Distribution ◽  
Abdominal Adhesions ◽  
Related Factors ◽  
Hypoxic Conditions ◽  
Significant Difference ◽  
Group A ◽  
One Year ◽  
Endothelial Growth Factor

The regulatory role between ischemia related factors and antimicrobial peptides in congenital intra-abdominal adhesions has not yet been defined. The aim of this research was to investigate the appearance and relative distribution of VEGF, HBD-2, and HGF in congenital intra-abdominal adhesions compared with relatively healthy tissue controls. The study group material was obtained from 48 patients who underwent abdominal surgery due to partial or complete bowel obstruction. VEGF, HBD-2, and HGF were detected using immunohistochemistry methods and their relative distribution was evaluated by means of the semiquantitative counting method. The results were analyzed using nonparametric statistic methods. A moderate number of VEGF positive endotheliocytes were detected, but there was no statistically significant difference between the groups. In the experimental group, a moderate to high number of VEGF positive macrophages was observed. In control group tissues, such macrophages were seen in significantly lower number (U = 61.0, p = 0.001). The increase of VEGF positive cells indicates support of angiogenesis due to the hypoxic conditions in case of adhesion disease. The number of HBD-2 marked fibroblasts and macrophages was moderate to high, but only few positive endotheliocytes were observed. Persisting appearance of HBD-2 positive structures might be a result of the inflammatory process. Most specimens showed occasional HGF positive macrophages and fibroblasts and there was no statistically significant difference between the groups. The relatively weak appearance of HGF suggests that the lack of this factor promotes the formation of fibrotic changes in case of intra-abdominal adhesions.

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