scholarly journals The Effectiveness Fig Leaves (FICUS CARICA L) Steeping Water Towards SGOT SGPT Serum on Male Wistar Rats in Critical Hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 826-835
Author(s):  
Yosi Julianti ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Ficus Carica ◽  
Negative Group ◽  
Positive Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Food And Drink ◽  
One Way Anova

Critical Hepatitisis is the inflammation of the liver that is caused by viruses, alchol and drugs which are characterized by the increasing values of SGOT SGPT. The purpose of this study is to determine the effectiveness of fig leaves (Ficuss carica L) steeping water towards the decrease of SGOT SGPT serum on male wistar rats in critical hepatitis model. The object of this study is 30 male wistar rats with the age of 2-3 months and weight 180-200 grams and randomly divide into 3 groups, which are the negative group that is only by giving an usual food and drink, the positive group and the treatment groups are given paracetamol 120mg/ oral for 7 days. The treatment group is given 0.65 gram/ day of fig leaves steeping water for 7 days. One way ANOVA of SPSS version 24 is used to analyze this research. The result of the study shows that there is a significant difference in SGOT serum level between the treatment group, positive group and negative group (p<0,05). And there is a significant difference  in SGPT level between the treatment group and the positive group (p<0,05). The conclusion of this study is tin leaves (Ficus carica L) steeping water has an effect towards the decrease of SGOT SGPT in male Wistar rats in critical hepatitis model.

scholarly journals The Effectiveness of Cherry Leaves Decoction (Muntingia Calabura L) Towards SGOT SGPT Serum on Male Wistar Rats in Critical Hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 695-704
Author(s):  
Julia Victoria Catherina ` R ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Serum Level ◽  
Wistar Rats ◽  
Male Rats ◽  
Negative Group ◽  
Positive Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Food And Drink ◽  
Muntingia Calabura

Critical Hepatitisis is caused by viruses, alcohol and drugs which are characterized by the increasing values of SGOT SGPT. The aim of this study is to determine the effectiveness of decoction extracts of cherry leaves (Muntingia Calabura L) towards serum SGOT SGPT on male wistar rats in critical hepatitis model. The rats that have been used are in number of 30 male rats with the weight is 180-200 grams and the age of 2-3 months devide into 3 groups,which are negative groups, positive groups and treatment groups.Each groups consist of 10 rats. The negative group is given an usual food and drink. The positive group and the treatment group are induced with 120 mg/ oral paracetamol for 7 days to increase serum levels of SGOT SGPT. The treatment group is given a decoction therapy of 10 grams cherry leaves in 100cc of water that is given 3.6cc/oral for 7 days.While the positive group is only given a regular food and drink for 7 days.ANOVA test is conducted to analyze this study by using SPSS 24 version. It is found in the result of the study that there are significant differences in serum level of SGOT between the treatment group, positive group and negative group (p<0,05). There is a significant value difference of SGPT in the treatment group and the positive group (p<0,05). However, there is no significant difference between the level of SGPT in the treatment group and the negative group (p=0,121).The conclusion is cherry leaves decoction (Muntingia Calabura L) has an effect towards the decrease of SGOT SGPT serum level on male wistar rats in critical hepatitis model.  

scholarly journals Pengaruh C-Phycocyanin Terhadap Kadar Soluble Glikoprotein (Sgp130) Pada Trofoblas Tikus Wistar Yang Dipapar Interleukin-6 (IL-6)

2018 ◽  
Vol 6 (1) ◽  
pp. 23
Author(s):  
Elizabeth Haryanti ◽  
Harry K Gondo
Keyword(s):  
Treatment Group ◽  
Interleukin 6 ◽  
Wistar Rats ◽  
Control Group ◽  
Placental Development ◽  
Successful Pregnancy ◽  
Treatment Groups ◽  
Significant Difference ◽  
Post Test ◽  
One Way Anova

The placenta is a joint organ of the uterus surface mother and fetus. One of cytokines of the placenta is IL-6 which is essential for normal placental development and successful pregnancy. Sgp130 binds Interleukin-6-sIL-6R complex, so Interleukin-6 signal cannot be passed on. Both factors play an important role in pre-eclampsia which has high levels of IL-6 and sgp130. They are expected to be suppressed by administration of C-phycocyanin. The aim of this study to prove effect of C-phycocyanin on sgp130 levels among wistar rats’ trophoblasts exposed to IL-6. The design of the study was Experiment, Post Test Only Control, analyse with One way Anova. The samples were 25 rats divided four treatment groups. Dose of C-Phycocyanin (10mg, 20mg, 40mg). The results significant difference between control and treatment group IL-6, IL-6 + CPC10, IL-6 + CPC20. But there was no significant difference between control group and IL-6 + CPC40, sig value. 0.214 (sig. <0.05). Conclusion. There is an effect of C-Phycocyanin on Sgp130 levels in wistar rats’ trophoblasts exposed to IL-6 at a dose of C-Phycocyanin 40mg.  

scholarly journals THE EFFECT OF SINGLE- AND REPEATED- EXCESSIVE SWIMMING EXERCISE ON KIDNEY HISTOPATHOLOGY OF MALE RATS WISTAR STRAIN (Rattus norvegicus)

2020 ◽  
Vol 1 (1) ◽  
pp. 33
Author(s):  
Wenni Juniarni Tripani ◽  
Muhammad In’am Ilmiawan ◽  
Willy Handoko
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Exercise Group ◽  
Proximal Tubules ◽  
Swimming Exercise ◽  
Control Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Mean

Background: Physical exercise aims to improve or maintain physical fitness. However, excessive physical exercise may cause increase of oxidative stress which leads to cellular injury, including in the proximal tubules of kidney. This research aims to find out the effect of single- and repeated- excessive swimming exercise to the kidney histopathology of male wistar rats (Rattus norvegicus). Methods: This research was an experimental study with complete random and post test only control group design. Twenty seven male wistar rats were divided into three treatment groups: control group, single- excessive swimming exercise group (45 minutes for one day), and repeated- excessive swimming exercise group (45 minutes/day for seven days). In the end of the treatment, the kidney of the rats were taken to be analyzed by using hematoxylin-eosin stain. The mean of proximal tubules injury of kidneys were counted by two observers using blinded method. Data were analyzed by using one way anova test. Result: The analysis showed there was a significant difference in the mean percentage of proximal tubules injury between control group and single swimming group as well as the repeated swimming group (Anova p=0,000), also there was significant difference between the treatment groups (p=0,020). The highest average of proximal tubules injury was in the single swimming group. Conclusion: Single- and repeated- excessive swimming exercise causes the proximal tubules injury of kidney.

The Effects of Brotowali (Tinospora crispa) Extract on The Number of Macrophage Cells in Wistar Rats (Rattus norvegicus) Suffering from Periodontitis

2021 ◽  
pp. 3077-3081
Author(s):  
Tuti Kusumaningsih ◽  
Ira Arundina ◽  
Tantiana Tantiana ◽  
Rini Devijanti R ◽  
Indeswati Diyatri ◽  
...  
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Healing Process ◽  
Periodontal Tissues ◽  
Macrophage Cells ◽  
Group I ◽  
Significant Difference ◽  
Histological Slide ◽  
Post Hoc ◽  
One Way Anova

Background: Periodontitis is an inflammatory disease of the periodontal tissues usually provoked by Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) bacteria. In the periodontitis healing process, macrophages play a significant role in the inflammatory stage. Brotowali (Tinospora crispa) consists of flavonoids that can stimulate periodontitis healing. Methods: Brotowali extract was derived from dried brotowali stems, processed into powder, and macerated with ethanol 80%. After that, the extraction was performed in three different concentrations, which were 25%, 50%, and 100%. five groups of Wistar rats were divided into Healthy wistar rats (control negative) Wistar rats suffering from periodontitis (control positive), Wistar rats suffering from periodontitis treated with 0.1 ml of 25% brotowali extract (treatment I), suffering from periodontitis treated with 0.1 ml of 50% brotowali extract (treatment II), and Wistar rats suffering from periodontitis treated with 0.1 ml of 100% brotowali extract (treatment III). The histological slide of each sample then was made to determine the number of macrophages cells. Results: One-Way ANOVA analysis revealed a notable difference among the groups. Tukey HSD Test (Post Hoc Test) showed no significant difference between (treatment group I) and (treatment group II). Conclusion: Brotowali extract may influence macrophage cells count of Wistar rats caused by A. actinomycetemcomitans bacteria.

scholarly journals Effectiveness of boiled Comfrey Leaf (Symphytum Officinale L) on Ureum Creatinine Serum in Male Wistar Strain of Acute Kidney Failure model.

2019 ◽  
Vol 7 (1) ◽  
pp. 705-715
Author(s):  
Pelagia Sedyati ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Kidney Failure ◽  
Male Rats ◽  
Acute Kidney Failure ◽  
Failure Model ◽  
Negative Group ◽  
Positive Group ◽  
Symphytum Officinale ◽  
Wistar Strain ◽  
Significant Difference

Acute kidney failure is a decrease in kidney that occurs only in a matter of hours even days which can be seen from decreased urine production and increased blood urea nitrogen and creatinine. This study aims to determine the effectiveness of boiled comfrey leaf (symphytum officinale L) on Ureum Creatinine Serum in Male Wistar Strain of Acute Kidney Failure model. This research used 30 male Wistar strain rats weighing 180-200 grams and aged 2-3 months that were divided into 3 groups, namely negative group (only given minerals), positive group (kidney damaged by gentamicin 0.3cc IP / day) and the treatment group (the kidneys were damaged with gentamicin 0.3cc IP / day and 2.8 g of boiled comfrey leaf in 150 cc of water and 2.7 cc were given orally for 7 days). Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare the levels of ureum and creatinine. The results showed that there was a significant difference in the ureum of the treatment group and the positive group (p <0.05) but there was no significant difference in the ureum value between the treatment group and the negative group. There was a significant difference in creatinine values between the treatment group, positive group and negative group (p <0.05). As a Conclusion boiled comfrey leaf (symphytum officinale L) water has an effect on decreasing serum levels of ureum creatinine in male rats wistar strain of acute kidney failure model.

scholarly journals Perbedaan efek pemberian tunggal vitamin E dengan pemberian kombinasi vitamin e dan zink terhadap kualitas spermatozoa tikus wistar (Rattus Norvegicus) yang diberi paparan asap rokok

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Iis A.H. Silalahi ◽  
Grace L.A. Turalaki ◽  
Janette M. Rumbajan
Keyword(s):  
Free Radicals ◽  
Vitamin E ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Enzymatic Antioxidant ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract: Smoking can increase free radicals in sperm, which can damage sperm. Vitamin E as non-enzymatic antioxidant and zinc as enzymatic antioxidant that is effective in dealing with free radicals. The aim of this research was to look at the differences between the effects of vitamin E and zinc on spermatozoa quality of male wistar rats (Rattus norvegicus) after exposure to cigarette smoke. The research used a completely randomized experimental design. Samples were 9 male wistar rats (Rattus norvegicus) which were randomly divided into 3 groups. Each group was given exposure to cigarette smoke from 2 bars of cigarette/day, which treatment group (P1) also was given vitamin E 1,44 mg/day and treatment group (P2) also was given combination of vitamin E 1,44 mg/day and zinc 10 mg/day. The treatment was given for 50 days. The results of this research showed a significant difference in the motility and morphology of spermatozoa between the treatment group (P1) which received exposure the cigarette smoke and vitamin E and treatment group (P2) which received exposure to cigarette smoke and combination of vitamin E and zinc (p<0,05) compared with the group which received exposure only cigarette smoke without vitamin E or zinc. The motility of spermatozoa treatment group (P2) which received exposure the cigarette smoke and combination vitamin E and zinc was found significant difference with the treatment group (P1) which received exposure the cigarette smoke and vitamin E (p <0,05). Conclusion: The conclusion from this research showed that there is difference between spermatozoa quality especially motility and morphology of group treated with vitamin E and combination of vitamin E and zinc after exposure to cigarette smoke, with combination of vitamin E and zinc had an higher average on spermatozoa quality especially motility.Keywords: vitamin E, zinc, cigarette smoke, quality of spermatozoa Abstrak: Merokok dapat menyebabkan peningkatan radikal bebas yang dapat merusak sperma. Vitamin E sebagai antioksidan non enzimatis dan zink sebagai antioksidan enzimatis merupakan antioksidan yang efektif dalam mengatasi radikal bebas. Tujuan penelitian ini adalah untuk melihat perbedaan efek antara pemberian vitamin E dan kombinasi vitamin E dengan zink terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) jantan setelah diberi paparan asap rokok. Penelitian ini menggunakan metode penelitian eksperimental dengan menggunakan rancangan acak legkap. Sampel yang digunakan adalah 9 tikus wistar (Rattus norvegicus) jantan yang dibagi secara acak menjadi 3 kelompok. Masing-masing kelompok tikus diberi paparan asap rokok 2 batang / hari dimana kelompok perlakuan (P1) juga diberi vitamin E 1,44 mg/hari, dan kelompok perlakuan (P2) diberi kombinasi vitamin E 1,44 mg/hari dan zink 10 mg/hari. Perlakuan diberikan selama 50 hari. Hasil penelitian menunjukkan adanya perbedaan signifikan pada motilitas dan morfologi spermatozoa kelompok perlakuan (P1) dengan perlakuan paparan asap rokok dan vitamin E dan kelompok perlakuan (P2) dengan perlakuan paparan asap rokok dan kombinasi vitamin E dengan zink dibandingkan dengan kelompok control (P0) yang hanya mendapat paparan asap rokok (p<0,05). Motilitas spermatozoa kelompok perlakuan (P2) didapati hasil yang berbeda signifikan dibandingkan dengan kelompok perlakuan (P1) (p<0,05). Simpulan: Kesimpulan dari penelitian ini didapatkan bahwa terjadi perbedaan signifikan kualitas spermatozoa yaitu pada motilitas dan morfologi antara pemberian tunggal vitamin E dan kombinasi vitamin E dengan zink setelah pemaparan asap rokok, dengan kombinasi vitamin E dan zink memiliki rata-rata kualitas spermatozoa lebih tinggi khususnya motilitas.Kata kunci: vitamin E, zink, asap rokok, kualitas spermatozoa

scholarly journals EFEK EKSTRAK MELON (Cucumis melo) dan GliadinTERHADAP KADAR Hb DAN HbCO TIKUS WISTAR JANTAN YANG DIPAPAR ASAP ROKOK

2017 ◽  
Vol 11 (1) ◽  
pp. 78
Author(s):  
Yuyun Erlina Susanti ◽  
Bambang Wirjatmadi
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Cucumis Melo ◽  
Wistar Rats ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

The exposure of cigarette smoke produce CO bond in hemoglobin. Melon (Cucumis melo) and gliadin contains of antioxidants that prevent tissue damage. The aim of this study was to analyze the difference of hemoglobin and carboxyhemoglobin concentration in male Wistar rats were exposed  to cigarette smoke and had been treated melon extract (Cucumis melo) and gliadin. The study was a laboratory experimental design, using Post Test Control Group Design Research and RAL method (Complete Randomize Design). The sample in this study were 25 male Wistar rats aged 3 months. The research was divided into 5 groups with 5 different treatment : control group, the treatment group were given exposure to cigarette smoke, the treatment group were given of cigarette smoke exposure and melon extract (Cucumis melo) and gliadin dose of 3 IU / day, 4.5 IU / day, and 9 IU / day.The subject were divided into 5 groups each treated for 28 day. The sample size used Federer formula. The collection of data was obtained from the results of laboratory tests to hemoglobin and Carboxyhemoglobin. Hb and HbCO data collected and analyzed by Manova test at 95% confidence level. The results showed significant difference in average Hb and HbCO concentration (p-value = 0.000) between male Wistar rats treated and not treated with melon extract (Cucumis melo) and gliadin. Melon extract has an effect on the decline of HbCO concentration due to exposure to cigarette smoke.Keywords:smoke, melon extract, Hb, HbCO

scholarly journals Pengaruh madu terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) yang diberi paparan asap rokok

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Pretty P. Makasenda ◽  
Janette M. Rumbajan ◽  
Grace L.A. Turalaki
Keyword(s):  
Reactive Oxygen Species ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract: Cigarettes are composed of hazardous chemicals such as nicotine, tar and carbon monoxide (CO) to name a few. In just a single puff of a cigarette, there are 1014 free radical molecules also known as Reactive Oxygen Species (ROS) that can cause sperm damage. Various natural ingredients native to Indonesia were found to contain various antioxidants, one of them is honey. The effects of honey as antioxidant may protect body cells in neutralizing free radicals caused by smoking and reducing the damage to spermatozoa cell that is caused by ROS and thereby avoiding the declining quality of spermatozoa. This study aimed to determine the effect of honey on the quality of spermatozoa of Wistar rats (Rattus norvegicus) that had been exposed to cigarette smoke. Subjects of this study were nine male Wistar rats (Rattus norvegicus) randomly divided into three groups weighing 150-200 g with the age range of 12-14 weeks. Each group of mice was given exposure to the smoke of 2 cigarettes a day in which the treatment group (P1) is also given 0.5 ml of honey per day, and the treatment group (P2) honey 1 ml / day. The results showed that honey treatment can improve concentration, motility, and morphology of spermatozoa Wistar rats (Rattus norvegicus) by exposure to cigarrets smoke. Occurred a significant difference of concentration, motility, and morphology of spermatozoa group given only the exposure to cigarette smoke and the group given exposure to cigarette smoke and honey. This results showed that 1 ml of honey per day could improve the quality of spermatozoa.Keywords: honey, cigarrets smoke, spermatozoa Abstrak: Rokok mengandung bahan kimia yang berbahaya, yaitu nikotin, tar dan gas karbon monoksida (CO). Dalam satu kali hisapan rokok terdapat 1014 molekul radikal bebas atau Reactive Oxygen Species (ROS) yang dapat merusak spermatozoa. Berbagai bahan alam asli Indonesia banyak mengandung antioksidan, salah satunya pada madu. Efek madu sebagai antioksidan dapat melindungi sel-sel tubuh termasuk menetralisir radikal bebas yang disebabkan oleh rokok dan mengurangi kerusakan sel spermatozoa yang disebabkan oleh ROS sehingga menghindari menurunnya kualitas spermatozoa. Penelitian ini bertujuan untuk mengetahui pengaruh madu terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) yang diberi paparan asap rokok. Subjek penelitian ini menggunakan 9 ekor tikus wistar (Rattus norvegicus) jantan yang dibagi menjadi tiga kelompok secara acak dengan berat badan 150-200 gram dan berumur 12-14 minggu. Masing-masing kelompok tikus diberi paparan asap rokok 2 batang / hari dimana kelompok perlakuan (P1) juga diberi madu 0.5 ml / hari, dan kelompok perlakuan (P2) diberi madu 1 ml / hari. Hasil penelitian memperlihatkan bahwa pemberian madu dapat meningkatkan konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar (Rattus norvegicus) yang diberi paparan asap rokok. Terjadi perbedaan yang bermakna dari konsentrasi, motilitas, dan morfologi spermatozoa kelompok yang hanya diberi paparan asap rokok dan kelompok yang diberi paparan asap rokok dan madu. Hasil tersebut menunjukan bahwa madu 1 ml / hari dapat meningkatkan kualitas spermatozoa. Kata kunci: madu, asap rokok, spermatozoa

scholarly journals Oral Candidiasis in Immunosuppressed Wistar Rats (Rattus norvegicus) Post Dexamethasone Injection at 7.2 mg/kg and 16 mg/kg Doses

2020 ◽  
Vol 23 (4) ◽  
pp. 7p
Author(s):  
Ucy Nur Hamida Al Abrori ◽  
Nurina Febriyanti Ayuningtyas ◽  
Diah Savitri Ernawati ◽  
Hening Tuti Hendarti ◽  
Meircurius Dwi Condro Surboyo ◽  
...  
Keyword(s):  
Treatment Group ◽  
Group Treatment ◽  
Wistar Rats ◽  
Oral Candidiasis ◽  
Control Group ◽  
Clinical Appearance ◽  
Significant Difference ◽  
Group 2 ◽  
Hyphal Formation ◽  
One Way Anova

Objective: Oral candidiasis is an infection that occurs in the oral cavity and is caused by candida species, often Candida albicans. This infection commonly occurs in a condition of immunosuppression caused by dexamethasone. Due to the side effects of antifungal therapy, developing a standardized immunosuppressed animal model to induce oral candidiasis for new therapies is required. The aim of this study is to observe oral candidiasis in immunosuppressed Wistar rats post dexamethasone injection at 7.2 mg/kg and 16 mg/kg doses. Material and Methods: Twenty-one Wistar rats were divided into three groups: control group, treatment group 1 (injected with dexamethasone at a concentration of 7.2 mg/kg), and treatment group 2 (at a concentration of 16 mg/kg) for five days. Immunosuppression status was observed by leukocyte count and all the subjects’ palates were inoculated with C.albicans 0.1 ml of 15x108 UFC/ml 24 hours later. The subjects’ tongues were observed and confirmed by laboratory examination on day 10. A statistical analysis was performed using one way ANOVA, Kruskal–Wallis, Tukey HSD, and Mann-Whitney U tests. Results: A significant clinical appearance of the subjects’ tongues was observed only between C and T1 (p=0.023;p<0.05). Significant hyphal formation was observed between C and T1 (p= 0.037;p<0.05) and between C and T2 (p=0.007;p<0.05), and no significant difference was observed between T1 and T2. A significant increase in the colony count was also observed in similar results. Conclusion: Dexamethasone injection at doses of 7.2 mg/kg and 16 mg/kg is effective in triggering immunosuppression to induce oral candidiasis in immunosuppressed Wistar rats. Keywords Dexamethasone; Immunosuppression; Oral candidiasis.

scholarly journals THE EFFECT OF RAMANIA LEAF (Bouea macrophylla Griff) EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN INCISIONAL WOUND OF MALE WISTAR RATS

2021 ◽  
Vol 6 (1) ◽  
pp. 96
Author(s):  
Dinda Rizkia ◽  
Irham Taufiqurrahman ◽  
Renie Kumala Dewi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Collagen Fibers ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Post Hoc

ABSTRACTBackground: Ramania leaf (Bouea macrophylla Griff) extract gel has secondary metabolites in the form of flavonoids, steroids, phenols and terpenoids which have a role as antioxidant. They will protect the body from excessive production of reactive oxygen species (ROS) by increasing endogenous antioxidants SOD, CAT and GPX, so that wound healing will not be inhibited and the process of collagen synthesis can run smoothly. Objective: To analyze the effect of ramania leaf extract gel that was applied topically with 5%, 10% and 15% concentration on collagen fibers density in incisional wound of male Wistar rats (Rattus norvegicus) on the 7th and the 14th day. Method: This research is a pure experimental study with a posttest-only control group design, using 24 rats which were divided into 4 groups: the treatment groups given ramania leaf extract gel of 5%, 10%, 15%, and the control group given placebo gel. The application of the extract gel was done once within 24 hours. The collagen level was measured with a spectrophotometer on the 7th and the 14th day. Results: Two-Way ANOVA test results on the 7th and the 14th day of each group showed a significant difference with p=0.000 (p<0.05). The Bonferroni Post-hoc Test showed a significant difference with p<0.05 between the placebo gel group and the groups of ramania leaf extract gel of 5%, 10%, 15% on the 7th and the 14th day. Conclusion: There is an effect of ramania leaf extract gel on collagen fibers density with the most effective concentration of 15%.Keywords: Antioxidant, , Collagen, Ramania Leaf Extract Gel

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