Analysis of Vascular Endothelial Growth Factor (VEGF) -634C/G and +936C/T Polymorphisms and Serum VEGF Levels in Women Suffering From Preeclampsia

Abstract Preeclampsia (PE) is a syndrome related with pregnancy and characterized by hypertension and proteinuria, occurring in approximately 6-8% of pregnancies and accounting for approximately 40% of premature births. This study aimed to investigate the polymorphisms of -634C/G and +936C/T in VEGF gene and their relationship with serum VEGF levels in pregnant women with PE. In this case-control study, peripheral blood samples were collected from 135 women with PE and 135 normal pregnant women as the control group. DNA was extracted using the phenol-chloroform method. Then, the polymorphisms of VEGF gene were detected by PCR-RFLP method using specific primers. Besides, VEGF concentrations were measured by ELISA method on serum samples and control subjects using ELISA kits. In this research, maternal age, gestational week, maternal hemoglobin and BMI were significantly correlated with the likelihood of PE, while the occurrence season variable was not effective in PE among the pregnant women. There was no significant difference in the two polymorphisms of -634C/G and +936C/T in VEGF gene between the two groups. Also, the serum VEGF level in PE patients was significantly higher than the normal group (P<0.001). Despite a significant increase in serum VEGF concentrations in women with PE, it seems that -634C/G and +936C/T polymorphisms of VEGF gene are not related with the onset of PE. Further studies are required to fully understand the risk factors related to preeclampsia syndrome.

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Effect of Serum Copper on Circulating Angiogenesis-Related Factors in Women with Preeclampsia

10.20944/preprints201909.0252.v1 ◽

2019 ◽

Author(s):

Khalid Najm Nadheer ◽

Zohreh Zahraei ◽

Hussein Al-Hakeim

Keyword(s):

Pregnant Women ◽

Serum Copper ◽

Control Group ◽

Vascular Endothelial ◽

Related Factors ◽

Soluble Endoglin ◽

Iraqi Women ◽

Endothelial Integrity ◽

And Control ◽

Endothelial Growth Factor

Preeclampsia (PE) is characterized by a series of clinical features such as hypertension and proteinuria associated with endothelial dysfunction and the impairment of placenta vascular endothelial integrity. This study aimed to investigate the effect of serum copper (Cu) level on some angiogenesis-related factors including vascular endothelial growth factor-A (VEGF-A), soluble Fms-like tyrosine kinase-1 (sVEGF-R1), soluble endoglin (sEng) and cerruloplasmin (Cp) in Iraqi women with preeclampsia (PE) and control pregnant women. Therefore, 60 women with PE in addition to 30 healthy pregnant women were enrolled in the study. Serum concentration of sEng, VEGF-A, sVEGF-R1, and Cu in PE group significantly increased (p<0.05) in the PE group compared with that in the control group. Increased production of antiangiogenic factors, soluble VEGF-A and sEng contribute to the pathophysiology of PE, indicating the involvement of these parameters in the angiogenic balance in patients with PE. Tests for between-subject effects showed that the circulating angiogenesis factors and Cu were significantly associated with the presence of PE. Serum Cu level was significantly correlated with VEGF- A and VEGF-R1 levels but not with sEng. Multiple regression analysis revealed that only Cp and BP can significantly predict the complications in women with PE. In conclusion, serum Cu has a role in the angiogenesis in women with PE and may be a new drug target in the prevention or treatment of PE.

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Comparison of serum vascular endothelial growth levels between patients with and without ovarian malignancies

International Journal of Gynecological Cancer ◽

10.1136/ijgc-00009577-200211000-00005 ◽

2002 ◽

Vol 12 (6) ◽

pp. 715-719 ◽

Cited By ~ 1

Author(s):

K. Dehaven ◽

D. D. Taylor ◽

C. Gercel-Taylor

Keyword(s):

Ovarian Cancer ◽

Mean Value ◽

Control Group ◽

Vascular Endothelial ◽

Serum Samples ◽

Ovarian Cancer Screening ◽

Significant Difference ◽

The Us ◽

The Mean ◽

Endothelial Growth Factor

The objective of this study was to determine if there was a relationship between serum vascular endothelial growth factor (VEGF) levels and ovarian malignancies by contrasting a population with ovarian malignancies and a population free of gynecological neoplasms. Two hundred forty four serum samples were obtained from the US National Cancer Institute's Prostate, Lung, Colon, and Ovarian Cancer Screening Project. These samples were analyzed by enzyme-linked immunosorbent assays in duplicate, and on completion of the assays, the samples were decoded for age and disease type. Average VEGF values for the nongynecological control group was 4.399 ng/ml; for benign gynecologic cases, 2.515 ng/ml; and for patients with malignancies, 4.287 ng/ml. Specifically, there was no difference between the mean value of VEGF in patients with ovarian malignancies and the patients with benign gynecological tumors (P = 0.8823). Also, there was no difference between the mean value of VEGF in patients with ovarian malignancies and the control patients who did not have gynecological disease (P = 0.3110). Using the Mann–Whitney U-test, no significant differences were found between the three populations of this study. Based on our data, due to the lack of significant difference in mean serum VEGF values between patients with and without ovarian malignancies, we feel that serum VEGF cannot be used as a possible screening tool for ovarian cancer.

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Vascular endothelial growth factor gene variations as a risk predictor in disc degeneration

Einstein (São Paulo) ◽

10.1590/s1679-45082017ao4053 ◽

2017 ◽

Vol 15 (4) ◽

pp. 403-408

Author(s):

Aline Amaro ◽

Ana Beatriz Guerra ◽

Matheus Pippa Defino ◽

Luiz Angelo Vieira ◽

Carla Peluso ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Disc Degeneration ◽

Control Group ◽

Risk Haplotype ◽

Vascular Endothelial ◽

Vegf Gene ◽

Risk Predictor ◽

And Control ◽

Endothelial Growth Factor

ABSTRACT Objective: To evaluate the frequency of polymorphisms in the vascular endothelial growth factor (VEGF) gene, as well as to identify a potential risk haplotype among the polymorphic regions in this gene in patients with disc degeneration and in the Control Group. Methods: This study analyzed a total of 217 individuals distributed into the Disc Degeneration and Control Groups. Peripheral blood was collected from all patients to detect VEGF gene polymorphisms identified by qPCR (rs699947, rs1570360, rs2010963, rs833061 and rs3025039). All patients presenting disc degeneration had the confirmation by nuclear magnetic resonance test and were rated according to disc degeneration level. Results: All polymorphisms were in Hardy- Weinberg equilibrium (p>0.05) in the studied population. The genotypic frequency for Disc Degeneration and Control Group were rs699947 p = 0.475, rs1570360 p = 0.862, rs2010963 p = 0.823, rs833061 p=0.596 and rs3025039 p=0.230. In haplotype analysis, the compositions CAGGC (p=0.094) and CCGGC (p=0.054) stood out. Conclusion: The correlation between VEGF gene polymorphism as a risk predictor for disc degeneration was negative in the studied population. However, the VEGF gene has a large polymorphic region, and it is activated by various catabolic and metabolic factors in the disc degeneration process, which has not been fully elucidated.

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Investigating the Effect of Positive, Intermediate and Negative Enabling and Training Factors Affecting Physical Activity in Pregnant Women

10.21203/rs.3.rs-151184/v1 ◽

2021 ◽

Author(s):

Leila Kianfard ◽

Farkhonde Amin SHokravi ◽

Sakineh Rakhshanderou ◽

Shamsaddin Niknami

Keyword(s):

Physical Activity ◽

Pregnant Women ◽

Control Group ◽

Control Groups ◽

Enabling Factors ◽

Factors Affecting ◽

Significant Difference ◽

E Learning ◽

And Control ◽

Experimental Group

Abstract BackgroundPhysical activity during pregnancy period is one of the issues with priority during pregnancy period. Researches show that women reduce their physical activity during this period and are unaware of the benefits on the health of the mother and embryology. Although researchers regarding physical activity during pregnancy have prepared many guidelines; it is not however clear why pregnant women do not perform physical activity and the effectiveness factors that facilitate the desired behavior. MethodsThe research population included all pregnant women aged 18 to 40 years with gestational age of 12-38 weeks referring to the healthcare centers in District 5 of Tehran Municipality, from 22 districts of the city who had eliminated their physical activity during pregnancy. Questionnaires of physical activity assessment questionnaire after educational intervention in pregnant women (PPAQ) and a questionnaire designed based on the results of the needs assessment and the dimensions of the PEN-3 model were used for assessment. This study is a Research Clinical Trial (RCT). Due to the nature, a quasi-experimental research design (pre-test, post-test) with the control group was used in this research. ResultsBased on the obtained results, the intervention based on E-learning has a significant effect of enabling factors to increase physical activity in pregnant women in the experimental group. In addition, the intervention based on E-learning has a significant effect on the training factors in order to increase physical activity in pregnant women in the experimental group. ConclusionBased on the research results, it can be said that there is a statistically significant difference between the experimental and control groups in the scores obtained from training factors in the pretest and the experimental group. Furthermore, it can be said that there is a statistically significant difference between the experimental and control groups in the scores obtained from the enabling factors in the pre-test and the experimental group.

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A study of vascular endothelial growth factor in the cord blood of pre-eclamptics and healthy pregnant women

Asian Journal of Medical Sciences ◽

10.3126/ajms.v8i1.15711 ◽

2017 ◽

Vol 8 (1) ◽

pp. 21-25

Author(s):

Anita Rawat ◽

Anil Kumar Gangwar ◽

Archana Ghildiyal ◽

Neena Srivastava ◽

Sunita Tiwari ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Cord Blood ◽

Pregnant Women ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Vascular Endothelial ◽

Cord Serum ◽

Endothelial Growth Factor

Background: Pre-eclampsia(PE) is the most frequently encountered medical complication during pregnancy. In developing countries PE is a principal cause of maternal mortality. A disturbance in the angiogenic/antiangiogenic factors and in the hypoxia/placental re-oxygenation process, seems to activate a maternal endothelial dysfunction.Aims and Objective: To estimate Vascular Endothelial Growth Factor ( VEGF ) level in the cord blood of healthy and Preeclamptic ( PEc ) pregnant women and to associate this with Preeclamptic pregnancy.Material and Methods: A case-control study ofUmbilical cord serum VEGF levels from women with uncomplicated pregnancies (control group, n=60) and pregnancies complicated by Pre-eclampsia (n=40). VEGF in the cord serum was estimated by SANDWICH Enzyme Linked Immunosorbent Assay method by using ELISA Kit and then compared between the two groups.Results: The mean VEGF concentrations in the women who had pre-eclampsia (578.62±468.3) were lower than in the control group( 625.75±533.1) , but the difference was not statistically significant ( p= 0.8548). Conclusion VEGF plays a key role in the instability between endothelial dysfunction and angiogenesis that occurs during Preeclampsia. VEGF levels might be a useful tool for the early diagnosis of Pre-eclampsia.Asian Journal of Medical Sciences Vol.8(1) 2017 21-25

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Association of the waist-hip ratio with plasma adiponectin related to coronary artery disease

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v17i2.35886 ◽

2018 ◽

Vol 17 (2) ◽

pp. 290-295

Author(s):

Premtim Rashiti ◽

Ibrahim Behluli ◽

Albiona Bytyçi

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Medical Science ◽

Control Group ◽

Serum Samples ◽

Waist Hip Ratio ◽

Significant Difference ◽

Artery Disease ◽

A Prospective Study ◽

And Control

Objective: By enrolling a prospective study of 82 patients that underwent non-urgent coronary angiography for coronary artery disease (CAD), it is aimed to investigate the correlation between adiponectin and waist-hip-ratio with severity of CAD.Materials and methods: The results of the angiography, divided the patients into two groups, patients admitted with a diagnosis of CAD and non-CAD. In the conducted hospital based research, two groups were involved: the study group with documented angiographically CAD and control group without angiographic evidence of CAD. Some of the baseline adiponectin levels in stored serum samples of all patients, anthropometric and biochemical risk factors were assessed in both groups.Result and discussion: As the result, we have seen the presence of CAD that was associated with current smoking, male gender, waist–hip ratio (WHR).While, no significant difference between median adiponectin levels at baseline were observed between cases and controls.Conclusion: There is a significant positive correlation between waist - hip ratio and presence of severity of coronary artery disease.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.290-295

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A Comparative Study of Relationship between Micronutrients and Gestational Diabetes

ISRN Obstetrics and Gynecology ◽

10.5402/2012/470419 ◽

2012 ◽

Vol 2012 ◽

pp. 1-4 ◽

Cited By ~ 14

Author(s):

Farideh Akhlaghi ◽

Seyyed Majid Bagheri ◽

Omid Rajabi

Keyword(s):

Gestational Diabetes ◽

Pregnant Women ◽

Gestational Age ◽

Control Group ◽

Study Group ◽

Inclusion Criteria ◽

Control Groups ◽

Significant Difference ◽

Micronutrient Concentration ◽

And Control

In this paper, we studied the relation between the micronutrient and gestational diabetes. Therefore, we measured micronutrient concentration including Ni, Al, Cr, Mg, Fe, Zn, Cu, and Se in serum of women with gestational diabetes between 24 and 28 weeks of gestational age (study group) who had inclusion criteria and comparison with micronutrient levels in normal pregnant women with same gestational age (control group). Results showed that there was no significant difference between the serum micronutrient level (Ni, Al, Cr, Mg, Zn, Cu, Se) in study and control groups except serum level of iron which in serum of gestational diabetic women was lower than normal pregnant women and difference was significant.

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Genetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian women

Human Genomics ◽

10.1186/s40246-021-00364-x ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Ochuwa Adiketu Babah ◽

Oyesola Oyewole Ojewunmi ◽

Akinniyi Adediran Osuntoki ◽

Melissa A. Simon ◽

Bosede Bukola Afolabi

Keyword(s):

Enzyme Linked Immunosorbent Assay ◽

Vascular Endothelial ◽

Nucleotide Polymorphisms ◽

Nigerian Women ◽

Pcr Rflp ◽

Hardy Weinberg Equilibrium ◽

Elisa Technique ◽

Genomic Dnas ◽

And Control ◽

Endothelial Growth Factor

Abstract Objective To determine if genetic polymorphism of VEGF is associated with the development of endometriosis in Nigerian women. Study design Case control study of 100 women (50 healthy controls and 50 with endometriosis). Serum VEGF concentration of participants were determined using enzyme-linked immunosorbent assay (ELISA) technique. Genomic DNAs were isolated from peripheral blood samples and quantified by nanodrop spectrophotometer one. Single nucleotide polymorphisms genotyping was carried out by polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP). Results Mean age of participants was 32.96 ± 6.91 years for control and 32.04 ± 7.56 years for cases. VEGF levels in case and control groups were not statistically different (82.68 pg/ml [69.11–121.11 pg/ml] vs. 82.81 pg/ml [72.90–113.82 pg/ml] respectively; p = 0.967). All four genotypes examined were in Hardy–Weinberg equilibrium. Minor allele frequency of − 460T > C, − 1154G > A, + 936C > T and + 2578C > A were 24%, 8%, 6% and 10% in the control and 19%, 9%, 5% and 14% in endometriosis patients. However, allele and genotype distributions of − 460T > C, − 1154G > A, + 936C > T and + 2578C > A VEGF polymorphisms in endometriosis patients and control were not significantly different (p > 0.05). Conclusion Our preliminary findings revealed no association between endometriosis and − 460T > C, − 1154G > A, + 936C > T and + 2578C > A of VEGF genes among Nigerian women.

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The effect of delivery ball and warm shower on the childbirth experience of nulliparous women: A randomized controlled clinical trial

10.21203/rs.3.rs-903099/v1 ◽

2021 ◽

Author(s):

parvaneh sharifipour ◽

Masoomeh Kheirkhah ◽

Mojgan Rajati ◽

hamid haghani

Keyword(s):

Clinical Trial ◽

Pregnant Women ◽

Control Group ◽

Controlled Clinical Trial ◽

Control Groups ◽

Childbirth Experience ◽

Nulliparous Women ◽

Significant Difference ◽

The Mean ◽

And Control

Abstract Background Childbirth is a unique experience that affects women’s life. Therefore, this study was performed to determine the effect of delivery ball and warm shower on the childbirth experience of primiparous women. Methods This study is a clinical trial that was carried out on primiparous pregnant women referred to Motazedi Hospital in Kermanshah, Iran. Sampling was done by continuous method and pregnant women were divided into three groups of delivery ball-warm shower (n = 33), delivery ball (n = 33) and control (n = 33). Exercise with ball at the dilation of 4 cm was similar in the two groups of delivery ball-warm shower and delivery ball, but the first group also used warm shower at the dilatation of 7 cm. The control group only received the routine delivery care. Demographic information form consisting of pregnancy history and information about the mother and infant were completed and the childbirth experience questionnaire (CEQ) were completed by the women two hours after the childbirth. Results There was a statistically significant difference in the mean score of childbirth experience after the intervention between the two groups of delivery ball-warm shower and control (P = 0.001), and also between the delivery ball and control groups (P = 0.001). There was a statistically significant difference in the mean scores of professional support between the two groups of delivery ball-warm shower and control (P = 0.02) and also between the delivery ball and control groups (p = 0.02). There was a statistically significant difference in the mean scores of participation between the two groups of delivery ball-warm shower and control (P = 0.003) and also between the delivery ball and control groups (P = 0.01). There was also a statistically significant difference in the mean scores of sense of security between the two groups of delivery ball-warm shower and control (P = 0.01). Conclusion Delivery ball and warm shower were effective interventions to create a positive childbirth experience. This method was more effective than using delivery ball alone in childbirth experience. To achieve a positive experience of childbirth in mothers, the use of both intervention (delivery ball and warm shower) is recommended.

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Is there a correlation between GAD2 gene-243 A>G polymorphism and obesity?

Revista Romana de Medicina de Laborator ◽

10.2478/rrlm-2019-0033 ◽

2019 ◽

Vol 27 (4) ◽

pp. 413-420

Author(s):

Camelia Alkhzouz ◽

Diana Miclea ◽

Marius Farcas ◽

Simona Bucerzan ◽

Georgiana Cabau ◽

...

Keyword(s):

Risk Factor ◽

Study Groups ◽

Normal Weight ◽

Control Group ◽

Case Group ◽

Subsequent Increase ◽

Significant Difference ◽

Pcr Rflp ◽

Transcriptional Effect ◽

And Control

Abstract Introduction: GAD2 gene encodes the glutamate decarboxylase enzyme which catalyses the transformation of glutamate into γ-aminobutyric acid, GABA. It is suggested that some polymorphic alleles of GAD2 gene, such as -243A>G, have an increased transcriptional effect compared with the wild type, which results in an increase of GABA in the hypothalamus with the subsequent increase of the neuropeptide Y, thus exacerbating the hunger centre and the appetite. The aim of this study was to observe an association between the -243A>G polymorphism with obesity, comparatively studying a group of obese patients and a group of patients with normal weight. Patients and method: 127 patients were clinically evaluated in the Genetic and Endocrine Department of Children’s Emergency Clinical Hospital, Cluj. The patients were included in two study groups, case group, with obesity (BMI higher than 97 kg/m2) and control group, with normal weight (BMI less than 97 kg/m2). Genotyping for GAD2-243A>G polymorphism was performed using PCR-RFLP technique, the two groups being compared regarding the genotypes and phenotypes. Results and conclusions: In the obesity group, there is a statistically significant difference in BMI (kg/m2) between the subgroups with different genotypes (p=0.01), the AA genotype being less severely affected than AG and GG genotypes. In the normal weight group there is no association between BMI and different genotypes (AA, AG or GG). Also, there is a greater distribution of GG genotypes and G allele in the obesity group compared with the control group, with an odds ratio which suggest that -243A>G polymorphism is a risk factor in obesity development (GG genotype OR=3.76, G allele OR=1.73, p=0.04). The finding of our study is important in explaining the multifactorial model of obesity, our research demonstrating that the GAD2-243 A> G variant could be a risk factor that added to other obesogenic factors would potentiate their effect.

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