scholarly journals Polyelectrolyte Complexes of Ciprofloxacin and Lidocaine Improve Wound Healing in Deep Second Degree Burns and Reduce in Vitro Ciprofloxacin Cytotoxicity in Fibroblasts

2022 ◽  
Author(s):  
María Florencia Sanchez ◽  
María Laura Guzman ◽  
Jesica Flores-Martín ◽  
Mariano Cruz Del Puerto ◽  
Carlos Laino ◽  
...  
Keyword(s):  
Superior Performance ◽  
Polyelectrolyte Complexes ◽  
Smart System ◽  
Smart Systems ◽  
Degree Burn ◽  
Ionic Complexation ◽  
New Treatments ◽  
Improve Wound Healing ◽  
Second Degree

Abstract The development of new treatments capable of controlling infections and pain related to burns continues to be a challenge. Antimicrobials are necessary tools, but these can be cytotoxic for regenerating cells.In this study, antibiotic-anesthetic smart systems obtained by ionic complexation of polyelectrolytes with ciprofloxacin and lidocaine were obtained as film and hydrogel. The complexation of ciprofloxacin with natural polyelectrolytes efficiently contributed to increasing biocompatibility in a primary culture of isolated fibroblasts. In addition, the relative levels of the proteins integrin β1 and p-FAK involved in cell migration were increased with no modifications in cell mobility. Their evaluation in a deep second-degree burn model revealed fast reepithelization, with appendage conservation and complete dermis organization. Encouragingly, we found that both the film and the hydrogel showed a significantly superior performance compared to the reference treatment of silver sulfadiazine cream. This work highlights the great potential of this smart system as an attractive dressing for burns, which surpasses currently available treatments.

scholarly journals Design and Testing of an Experimental Steam-Induced Burn Model in Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Vlad Porumb ◽  
Alexandru Florentin Trandabăț ◽  
Cristina Terinte ◽  
Irina Draga Căruntu ◽  
Elena Porumb-Andrese ◽  
...  
Keyword(s):  
Automated System ◽  
Level Of Automation ◽  
Exposure Temperature ◽  
Degree Burn ◽  
Computer Controlled ◽  
The Difference ◽  
High Level ◽  
New Treatments ◽  
Experimental Burns ◽  
Second Degree

Background. Most of the current models for experimental burns pose difficulties in ensuring consistency and standardization.Aim of Study. We aimed to develop an automated, reproducible technique for experimental burns using steam-based heat transfer.Methods. The system developed for steam exposure was based on a novel, integrated, computer-controlled design. Three groups of rats were exposed to steam for 1, 3, and 7 seconds. The lesions were evaluated after 20 minutes, 48 hours, and 72 hours after burn induction.Results. One-second steam application produced a superficial second-degree burn; three-second application induced deep second-degree burn; and seven-second application led to a third-degree burn.Conclusion. The high level of automation of our integrated, computer-controlled system makes the difference between our system and other models, by ensuring the control of the duration of exposure, temperature, and pressure and eliminating as many potential human generated errors as possible. The automated system can accurately reproduce specific types of burns, according to histological assessment. This model could generate the reproducible data needed in the study of burn pathology and in order to assess new treatments.

Plasma Contact Activation and Decrease of Factor V Activity on Negatively-Charged Polyelectrolytes

1984 ◽  
Vol 51 (01) ◽  
pp. 061-064 ◽  
Author(s):  
M C Boffa ◽  
B Dreyer ◽  
C Pusineri
Keyword(s):  
Time Dependent ◽  
Initial Contact ◽  
Factor Xii ◽  
Factor V ◽  
Contact Activation ◽  
Polyelectrolyte Complexes ◽  
Plasma Factor ◽  
Fresh Plasma ◽  
Direct Adsorption

SummaryThe effect of negatively-charged polymers, used in some artificial devices, on plasma clotting and kinin systems was studied in vitro using polyelectrolyte complexes.Contact activation was observed as an immediate, transient and surface-dependent phenomenon. After incubation of the plasma with the polymer a small decrease of factor XII activity was noticed, which corresponded to a greater reduction of prekallikrein activity and to a marked kinin release. No significant decrease of factor XII, prekallikrein, HMW kininogen could be detected immunologically. Only the initial contact of the plasma with the polyelectrolyte lead to activation, subsequently the surface became inert.Beside contact activation, factor V activity also decreased in the plasma. The decrease was surface and time-dependent. It was independent of contact factor activation, and appeared to be related to the sulfonated groups of the polymer. If purified factor V was used instead of plasma factor V, inactivation was immediate and not time-dependent suggesting a direct adsorption on the surface. A second incubation of the plasma-contacted polymer with fresh plasma resulted in a further loss of Factor V activity.

scholarly journals Cyclodextrin-Based Supramolecular Complexes of Osteoinductive Agents for Dental Tissue Regeneration

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 136
Author(s):  
Masahiko Terauchi ◽  
Atsushi Tamura ◽  
Yoshinori Arisaka ◽  
Hiroki Masuda ◽  
Tetsuya Yoda ◽  
...  
Keyword(s):  
Growth Factors ◽  
Bone Formation ◽  
Small Molecule ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Inclusion Complexation ◽  
Dental Tissue ◽  
Polyelectrolyte Complexes ◽  
Small Molecule Drugs

Oral tissue regeneration has received growing attention for improving the quality of life of patients. Regeneration of oral tissues such as alveolar bone and widely defected bone has been extensively investigated, including regenerative treatment of oral tissues using therapeutic cells and growth factors. Additionally, small-molecule drugs that promote bone formation have been identified and tested as new regenerative treatment. However, treatments need to progress to realize successful regeneration of oral functions. In this review, we describe recent progress in development of regenerative treatment of oral tissues. In particular, we focus on cyclodextrin (CD)-based pharmaceutics and polyelectrolyte complexation of growth factors to enhance their solubility, stability, and bioactivity. CDs can encapsulate hydrophobic small-molecule drugs into their cavities, resulting in inclusion complexes. The inclusion complexation of osteoinductive small-molecule drugs improves solubility of the drugs in aqueous solutions and increases in vitro osteogenic differentiation efficiency. Additionally, various anionic polymers such as heparin and its mimetic polymers have been developed to improve stability and bioactivity of growth factors. These polymers protect growth factors from deactivation and degradation by complex formation through electrostatic interaction, leading to potentiation of bone formation ability. These approaches using an inclusion complex and polyelectrolyte complexes have great potential in the regeneration of oral tissues.

scholarly journals Investigating the Impact of Collagen-Chitosan Derived from Scomberomorus Guttatus and Shrimp Skin on Second-Degree Burn in Rats Model

2021 ◽  
Vol 18 ◽  
pp. 12-20
Author(s):  
Mohammad Javad Fatemi ◽  
Soheila Naderi Garahgheshlagh ◽  
Tayyeb Ghadimi ◽  
Shahla Jamili ◽  
Mohammad Reza Nourani ◽  
...  
Keyword(s):  
Degree Burn ◽  
The Impact ◽  
Second Degree

scholarly journals Patent Analysis on the Development of the Shale Petroleum Industry Based on a Network of Technological Indices

Energies ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 6746
Author(s):  
Jong-Hyun Kim ◽  
Yong-Gil Lee
Keyword(s):  
Petroleum Industry ◽  
Development Pattern ◽  
Directional Drilling ◽  
Patent Data ◽  
Smart System ◽  
Smart Systems ◽  
Complex Development ◽  
Technological Developments ◽  
Technological Indices ◽  
Degree Of Convergence

This study investigated the technological developments in the shale petroleum industry by analyzing patent data using a network of technological indices. The technological developments were promoted by the beginning of the shale industry, and after the first five years, it showed a more complex development pattern with the convergence of critical technologies. This paper described progress in the shale petroleum technologies as changes in relatedness networks of technological components. The relatedness represents degree of convergence between technological components, and betweenness centrality of network represents priority of technological components. In the results, the progress of the critical technologies such as directional drilling, increasing permeability, and smart systems, were actively carried out from 2012 to 2016. Especially, unconverged technology of increasing permeability and the converged technology of directional drilling and smart system has been intensively developed. Some technological components of the critical technologies are more significant in the form of converged technology.

673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S193-S193
Author(s):  
Emre Karakaya ◽  
Aydincan Akdur ◽  
H Ebru ◽  
Ayvazoglu Soy ◽  
Alev Ok Atilgan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Male Rats ◽  
Control Group ◽  
Ozone Therapy ◽  
Burn Wounds ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p < 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

Wound Healing Activity of Avocado Peel Ointment against Second Degree Burn Wound

2021 ◽  
Author(s):  
Ma I Yang ◽  
Ermi Girsang ◽  
Ali Napiah Nasution ◽  
Chrismis Novalinda Ginting
Keyword(s):  
Wound Healing ◽  
Burn Wound ◽  
Degree Burn ◽  
Wound Healing Activity ◽  
Second Degree

The Use of Lectins as Tools to Combat SARS-CoV-2

2021 ◽  
Vol 27 ◽  
Author(s):  
Daniela Martinez ◽  
Diego Amaral ◽  
David Markovitz ◽  
Luciano Pinto
Keyword(s):  
Viral Infection ◽  
Host Cells ◽  
Viral Fusion ◽  
Cell Receptors ◽  
First Case ◽  
Mannose Binding ◽  
New Treatments ◽  
Viral Surface

Background: in december 2019, china announced the first case of an infection caused by an, until then, unknown virus: sars-cov-2. since then, researchers have been looking for viable alternatives for the treatment and/or cure of viral infection. among the possible complementary solutions are lectins, and proteins that are reversibly bound to different carbohydrates. the spike protein, present on the viral surface, can interact with different cell receptors: ace2, cd147, and dc-signr. since lectins have an affinity for different carbohydrates, the binding with the glycosylated cell receptors represents a possibility of preventing the virus from binding to the receptors of host cells. Objective: in this review we discuss the main lectins that are possible candidates for use in the treatment of covid-19, highlighting those that have already demonstrated antiviral activity in vivo and in vitro, including mannose-binding lectin, griffithsin, banlec, and others. we also aim to discuss the possible mechanism of action of lectins, which appears to occur through the mediation of viral fusion in host cells, by binding of lectins to glycosylated receptors found in human cells and/or binding of these proteins with the spike glycoprotein, present in virus surface.moreover, we also discuss the use of lectins in clinical practice. Conclusion: Even with the development of effective vaccines, new cases of viral infection with the same virus, or new outbreaks with different viruses can occur; so, the development of new treatments should not be discarded. moreover, the discussions made in this work are relevant regarding the anti-viral properties of lectins.

In vitro effectiveness of biapenem against IMP-producing Enterobacteriaceae

2021 ◽  
Vol 70 (10) ◽  
Author(s):  
Kazuyoshi Gotoh ◽  
Makoto Miyoshi ◽  
I Putu Bayu Mayura ◽  
Koji Iio ◽  
Osamu Matsushita ◽  
...  
Keyword(s):  
Therapeutic Option ◽  
Small Data ◽  
Limited Data ◽  
Data Set ◽  
Content Type ◽  
Link Type ◽  
Almost All ◽  
New Treatments ◽  
Time Kill

The options available for treating infections with carbapenemase-producing Enterobacteriaceae (CPE) are limited; with the increasing threat of these infections, new treatments are urgently needed. Biapenem (BIPM) is a carbapenem, and limited data confirming its in vitro killing effect against CPE are available. In this study, we examined the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of BIPM for 14 IMP-1-producing Enterobacteriaceae strains isolated from the Okayama region in Japan. The MICs against almost all the isolates were lower than 0.5 µg ml−1, indicating susceptibility to BIPM, while approximately half of the isolates were confirmed to be bacteriostatic to BIPM. However, initial killing to a 99.9 % reduction was observed in seven out of eight strains in a time–kill assay. Despite the small data set, we concluded that the in vitro efficacy of BIPM suggests that the drug could be a new therapeutic option against infection with IMP-producing CPE.

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