scholarly journals A newly isolated Cerrena unicolor capable of laccase production and lignin degradation in agricultural wastes

2020 ◽  
Author(s):  
Jiahui Tian ◽  
Jiali Feng ◽  
Ying Wang ◽  
Junhua Lu ◽  
Linjing Mao ◽  
...  
Keyword(s):  
Solid State ◽  
Enzymatic Hydrolysis ◽  
Solid State Fermentation ◽  
Lignin Degradation ◽  
Gene Sequencing ◽  
Laccase Production ◽  
Sequencing Technology ◽  
Cerrena Unicolor ◽  
Rice Stalks ◽  
18S Rdna Gene

Abstract Lignin is main residue of agro-industrial biomass which can be decomposed through enzymatic hydrolysis by fungi. In this study, a strain was isolated from birch forest and identified as Cerrena unicolor GC.u01 by 18S rDNA gene-sequencing technology. The activity of laccase (Lac) reached maximum 1605.28 ± 32.21 U·L-1 at 8th day via submerged fermentation, while the highest Lac activity by solid-state fermentation 1280.04 ± 48.11 U·g-1 with rice stalks and 566.83 ± 47.02 U·g-1 with wheat stalks were both obtained at 10th day, and 2677.50 ± 49.38 U·g-1 with corn stalks at 12th day. Then the lignin degradation ratios were up to 24.3%, 34.3% and 26.2% in wheat stalks, rice stalks and corn stalks, respectively, suggesting that the newly isolated Cerrena unicolor GC.u01 is potential for laccase production and lignin degradation by solid-state fermentation.

scholarly journals Solid-state fermentation with orange waste: optimization of Laccase production from Pleurotus pulmonarius CCB-20 and decolorization of synthetic dyes

2020 ◽  
Vol 42 ◽  
pp. e52699 ◽  
Author(s):  
Alex Graça Contato ◽  
Fabíola Dorneles In´ácio ◽  
Tatiane Brugnari ◽  
Caroline Aparecida Vaz de Araújo ◽  
Giselle Maria Maciel ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Laccase Activity ◽  
Fermentation Medium ◽  
Laccase Production ◽  
Synthetic Dyes ◽  
Pleurotus Pulmonarius ◽  
Biotechnological Potential ◽  
Orange Waste ◽  
Oxidoreductase Enzymes

Laccases are oxidoreductase enzymes that have the ability to oxidize phenolic substrates. Its biotechnological potential has been greatly explored in many areas as biotechnology industry, bioremediation of dyes, food industry and environmental microbiology. The aim of this study was maximize the laccase production by Pleurotus pulmonarius (Fr.) Quélet in solid-state fermentation (SSF) using orange waste as substrate. After optimization the capability of the crude laccase to decolorize dyes was analyzed. The fermentation medium in the solid-state was optimized by applying a factorial design. After statistics optimization, laccase activity increased two times. The laccase activity appears to be correlated with the ability of crude extract to decolorize some industrial dyes. The optimized laccase was characterized with respect to optimum pH, influence of temperature and salts. Our results demonstrate that P. pulmonarius was an efficient producer of an important industrial enzyme, laccase, in a cheap solid-state system using orange waste as substrate.

scholarly journals Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse byPleurotus ostreatusin Solid-State Fermentation

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Susan Grace Karp ◽  
Vincenza Faraco ◽  
Antonella Amore ◽  
Luiz Alberto Junior Letti ◽  
Vanete Thomaz Soccol ◽  
...  
Keyword(s):  
Solid State ◽  
Ferulic Acid ◽  
Ammonium Sulfate ◽  
Sugarcane Bagasse ◽  
Copper Sulfate ◽  
Yeast Extract ◽  
Solid State Fermentation ◽  
Laccase Activity ◽  
Oxidative Enzymes ◽  
Laccase Production

Laccases are oxidative enzymes related to the degradation of phenolic compounds, including lignin units, with concomitant reduction of oxygen to water. Delignification is a necessary pretreatment step in the process of converting plant biomass into fermentable sugars. The objective of this work was to optimize the production of laccases and to evaluate the delignification of sugarcane bagasse byPleurotus ostreatusin solid-state fermentation. Among eight variables (pH, water activity, temperature, and concentrations of CuSO4, (NH4)2SO4, KH2PO4, asparagine, and yeast extract), copper sulfate and ammonium sulfate concentrations were demonstrated to significantly influence laccase production. The replacement of ammonium sulfate by yeast extract and the addition of ferulic acid as inducer provided increases of 5.7- and 2.0-fold, respectively, in laccase activity. Optimization of laccase production as a function of yeast extract, copper sulfate, and ferulic acid concentrations was performed by response surface methodology and optimal concentrations were 6.4 g/L, 172.6 μM, and 1.86 mM, respectively. Experimentally, the maximum laccase activity of 151.6 U/g was produced at the 5th day of solid-state fermentation. Lignin content in sugarcane bagasse was reduced from 31.89% to 26.36% after 5 days and to 20.79% after 15 days by the biological treatment of solid-state fermentation.

Efficient Production of Laccase from Armillariella tabescens by Solid State Fermentation

2014 ◽  
Vol 1010-1012 ◽  
pp. 42-47
Author(s):  
Jun Yao He ◽  
Xuan Yi Ye ◽  
Qing Zhi Ling ◽  
Li Hui Dong
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Initial Moisture Content ◽  
Nitrogen Sources ◽  
Solid Substrate ◽  
Laccase Production ◽  
Efficient Production ◽  
Operational Variables

The production of laccase by solid-state fermentation (SSF) usingArmillariella tabescenswas studied. Wheat bran was selected to be the most suitable solid substrate. Several operational variables including nitrogen source, moisture content, copper and aromatic inducers were investigated. The results showed that the complex nitrogen sources, NH4NO3coupled with peptone was shown to be the best nitrogen source. 75% of initial moisture content was proved to be appropriate. Copper significantly influenced the laccase production and the yield of laccase was improved by addition of 1.5 mM copper sulphate in the medium. Guaiacol efficiently induced the laccase production and the enzyme yield (24500U/g) was enhanced by 32% compared with he control without any aromatic inducers. Efficient production of laccase fromA. tabescenscan be achieved by solid-state fermentation.

scholarly journals Enhancement of Laccase Production by Optimizing the Cultural Conditions for Pleurotus Sajor-Caju in Solid-State Fermentation

2021 ◽  
Author(s):  
Shradhdha Sharma ◽  
Duggirala Srinivas Murty
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Cost Effective ◽  
Inoculum Size ◽  
Laccase Production ◽  
Native Page ◽  
Industrial Sectors ◽  
Pleurotus Sajor Caju

Nowadays, a lot of interest has been given to the development of cost-effective and efficient enzyme production technologies. Laccase enzymes are widely used in biotechnological, environmental and industrial sectors. Due to the cost-effectiveness of the solid-state fermentation (SSF) process, it is widely used to produce a broad range of biological products. In this study, optimization of moisture content, temperature, pH, and inoculum size were studied to enhance laccase production ability of Pleurotus sajor-caju in SSF by using One Factor At Time (OFAT) and Response Surface Methodology (RSM). OFAT was used as a baseline study for deducing the experimental design of RSM. The highest production of laccase enzyme (1450 U/g) by Pleurotus sajor-caju on wheat straw was observed at 26°C, 6.0 pH, 72.5 % moisture content, 7.5% inoculum size, 1% fructose and 0.5 % peptone. Unlike the conventional inoculum preparation method, here the inoculum was generated by the spawning method for SSF. The molecular weight of partially purified laccase from Pleurotus sajor-caju was estimated to be around 62 K Da using SDS PAGE. The activity staining of laccase was observed as a zymogram on Native PAGE using ABTS as a substrate. Lignin degradation of wheat straw and its structural disruption due to laccase was observed by Scanning Electron Microscopy (SEM).

A comparative study on the laccase activity of four basidiomycete fungi with different lignocellulosic residues via solid-state fermentation

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3017-3031
Author(s):  
Mei-Ling Han ◽  
Qi An ◽  
Kai-Yue Ma ◽  
Wen-Ning An ◽  
Wen-Yao Hao ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Solid State ◽  
Comparative Study ◽  
Sorghum Bicolor ◽  
Solid State Fermentation ◽  
Laccase Activity ◽  
Industrial Applications ◽  
Laccase Production ◽  
Lignocellulosic Residues ◽  
Basidiomycete Fungi

The laccase producing abilities of four Basidiomycete fungi species were compared using solid-state fermentation using four different lignocellulosic residues. The biosynthetic potential of the Basidiomycetes was highly dependent on the type of fungi. In general, the laccase secreting ability of Cerrena unicolor Han 849 was greater than Lenzites betulinus Han 851, Stropharia rugosoannulata Han 1321, and Auricularia heimuer Han 1333. The maximum laccase production of C. unicolor Han 849 was approximately 11.25, 122.26, and 15.27 times higher than L. betulinus Han 851, S. rugosoannulata Han 1321 and A. heimuer Han 1333, respectively. Different species of fungi had a preference in lignocellulosic residues. The presence of Firmiana platanifolia was conducive to secreting laccase via C. unicolor Han 849 during solid-state fermentation. A continuous and stable laccase production via C. unicolor Han 849 was an obvious advantage of solid-state fermentation with any of the four lignocellulosic residues used. The maximum laccase production of C. unicolor Han 849 using Firmiana platanifolia was approximately 2.12, 1.68, and 6.13 times higher than Populus beijingensis, Sorghum bicolor, and Oryza sativa, respectively. These findings will be helpful for developing new productivity strains in industrial applications and selecting suitable lignocellulosic residues for laccase production.

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