The Effect of Transport Stress on Corticosterone Metabolites in Pheasant Droppings
Abstract BackgroundTransport has been proven to represent a significant stressor for animals. In view of the fact that pheasants are frequently reared in captivity in hatcheries and are then transported for the purpose of their sale or release, it is essential from the viewpoint of welfare and health protection to obtain as much information as possible about their response to this stressor. The aim of this study was to assess changes in corticosterone metabolite (CM) levels related to transport in common pheasants (Phasianus colchicus). MethodsSixteen birds aged 17 weeks were crated and transported for 3.5 hours (distance travelled 140 km) to the target customer. Individual droppings were collected during the 2 days prior to transport and 3 days after transport. Concentrations of faecal CMs were determined with a non-commercial EIA (enzyme-immunoassay) kit, cortisone was used as a standard and values were expressed as cortisone equivalents in ng per g of droppings. ResultsTransport represents a major stressor for animals and this was also reflected in increased CM concentrations in the droppings in our study. CM concentrations increased within 24 hours of transport (P < 0.01) as compared to pre-transport levels (533.82 ng/g vs. 228.85 ng/g) and continued to rise for another 24 hours (745.35 ng/g). Three days after transport CM concentrations fell back to baseline levels (297.97 ng/g). ConclusionsThe EIA used in this study proved to be sensitive enough to detect biologically meaningful alterations in the adrenocortical activity of pheasants exposed to procedures related to their transport.