Microbial Leakage Evaluation Of Warm Gutta – Percha Techniques

2020 ◽  
Author(s):  
Antonio Libonati ◽  
Anna Piccinno ◽  
Gianni Gallusi ◽  
Edoardo Montemurro ◽  
Virginia Di Taranto ◽  
...  
Keyword(s):  
Continuous Wave ◽  
Control Groups ◽  
Gutta Percha ◽  
Bacterial Leakage ◽  
Negative Controls ◽  
Observation Period ◽  
Positive Controls

Abstract Background: To compare bacterial leakage of MicroHeat and continuous wave with and without endodontic sealer. Methods: Thirty – eight single – rooted extracted mandibular premolars were selected and randomly divided into four experimental groups (n=8) and two control groups (n=3). Teeth were prepared with Mtwo NiTi files and obturated with MicroHeat or System B with or without endodontic sealer. Three teeth were used as positive controls (Ct+) and three intact teeth served as negative controls (Ct-). All samples were tested for bacterial infiltration every day for 60 days. Results: On day 32 overall contamination value was 62,5% for Mseal, 75% for Mnoseal, 75% for SBseal and 37,8% for SBnoseal; after 60 days, the final contamination result was 100% for Mseal, Mnoseal and SBseal and 87,5% for SBnoseal. Conclusions: At the end of the observation period, groups showed no statistically significant differences.

scholarly journals "In vitro" evaluation of the apical sealing of root canals obturated with different techniques

2003 ◽  
Vol 11 (3) ◽  
pp. 181-185 ◽  
Author(s):  
Viviane Haiub Brosco ◽  
Norberti Bernardineli ◽  
Ivaldo Gomes de Moraes
Keyword(s):  
Continuous Wave ◽  
The Other ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Root Canals ◽  
Gutta Percha ◽  
Sealing Ability ◽  
Better Than ◽  
Positive Controls

The purpose of this study was to compare the apical sealing of root canals obturated with different techniques. One hundred-six human mandibular incisors were submitted to instrumentation by means of the step-back technique. After instrumentation, one hundred teeth received an impermeable coating on the external surfaces of the crown and root (except for the area nearby the apical foramen). Afterwards, they were divided in five groups containing twenty elements each, according to the obturation technique employed: 1. lateral condensation with Kerr file; 2. continuous wave of condensation technique with System B; 3. thermoplasticized injectable gutta-percha technique with the Ultrafil system; 4. mechanically thermoplasticized gutta-percha with the JS Quick-Fill system and 5. thermoplasticized gutta-percha associated to a master cone with the Microseal system. The six remaining teeth were employed as negative and positive controls. After obturation, the access cavities were sealed and the teeth were immersed in aqueous 2% methylene blue dye for 72 hours at 37ºC. After that, the teeth were longitudinally sectioned and the apical microleakage was evaluated in a stereomicroscope. The Microseal system presented the best apical sealing ability, followed by System B, JS Quick-Fill, Ultrafil and the lateral condensation technique. The statistical analysis of the results demonstrated that: 1. the Microseal system presented an apical sealing similar to System B and better than the other groups; 2. System B presented better apical sealing than the lateral condensation technique, being similar to the other groups; and 3. the lateral condensation, Ultrafil and JS Quick-Fill groups demonstrated similar sealing ability.

Effect of Nano Biphasic Calcium Phosphate Bioceramics on Periodontal Regeneration in the Treatment of Alveolar Defects

2012 ◽  
Vol 486 ◽  
pp. 422-425 ◽  
Author(s):  
Lan Lei Wang ◽  
Ji Hua Li ◽  
Yong Tao Xie ◽  
Pi Shan Yang ◽  
Yun Mao Liao ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Bone Defects ◽  
Beagle Dogs ◽  
Control Groups ◽  
Negative Controls ◽  
Osseous Defects ◽  
Positive Controls

The aim of the present study is to investigate the efficacy of nanobiphasic calcium phosphate (nanoBCP) bioceramic in the treatment of periodontal osseous defects. Alveolar bone defects were surgically created bilaterally at the buccal aspects of four second premolars in two beagle dogs. After root leveling, nanoBCP was randomly filled in one defect and nothing was put into the contralateral defect as negative controls. Bioglass was randomly filled in one of left defect as positive controls and nothing was put into the contralateral defect as negative controls. Dogs were killed at the 12th week. Histological observations were processed through a light microscopy. The results reveal that a great amount of functional periodontal fissures formed in the defects in the nanoBCP groups and bioglass groups while no new bone formed in the control groups at all. In this study, nanoBCP has proven to work well as a biocompatible and bioactive material to promote periodontal regeneration effectively.

scholarly journals Disturbance of Cell Proliferation in Response to Mobile Phone Frequency Radiation

2009 ◽  
Vol 60 (1) ◽  
pp. 109-115 ◽  
Author(s):  
Ivančica Trošić ◽  
Ivan Pavičić
Keyword(s):  
Cell Proliferation ◽  
Mobile Phone ◽  
Mitotic Index ◽  
Specific Absorption Rate ◽  
Continuous Wave ◽  
V79 Cells ◽  
Dividing Cells ◽  
Negative Controls ◽  
Frequency Radiation ◽  
Positive Controls

Disturbance of Cell Proliferation in Response to Mobile Phone Frequency RadiationThe aim of study was to determine the influence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79 cells after 1 h, 2 h, and 3 h of exposure. V79 cells were cultured in standard laboratory conditions and exposed to continuous-wave (CW) RF/MW radiation of 935 MHz, electric field strength of (8.2±0.3) V m-1, and specific absorption rate (SAR) of 0.12 W kg-1. To identify proliferation kinetics, the cells were counted for each hour of exposure 24 h, 48 h, 72 h, and 96 h after respective exposures. Microtubule proteins were determined using specific immunocytochemical methods. Cell smears were analysed under a fluorescent microscope. The study included negative and positive controls. Mitotic index was determined by estimating the number of dividing cells 24 h after exposure and dividing it with the total number of cells. In comparison to the controls, cell proliferation declined in cells exposed for three hours 72 h after irradiation (p<0.05). Microtubule structure was clearly altered immediately after three hours of irradiation (p<0.05). The mitotic index in RF/MW-exposed cells did not differ from negative controls. However, even if exposure did not affect the number of dividing cells, it may have slowed down cell division kinetics as a consequence of microtubule impairment immediately after exposure.

scholarly journals Influenza Vaccine Effectiveness Estimates among US Department of Defense Adult Beneficiaries over Four Consecutive Influenza Seasons: A Test-Negative Design Study with Different Control Groups

Vaccines ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 58
Author(s):  
Wenping Hu ◽  
Paul A. Sjoberg ◽  
Laurie S. DeMarcus ◽  
Anthony S. Robbins
Keyword(s):  
Influenza Virus ◽  
Influenza Vaccine ◽  
Department Of Defense ◽  
Influenza A ◽  
Vaccine Effectiveness ◽  
Control Groups ◽  
Design Study ◽  
Influenza A H1n1 ◽  
Negative Controls ◽  
Positive Controls

A test-negative design study with different control groups (influenza test-negative controls, non-influenza virus positive controls, and pan-negative controls) was conducted to assess inactivated influenza vaccine effectiveness (VE) in adults aged ≥18 years, 2016–2017 through 2019–2020 influenza seasons. A database was developed from the US Department of Defense Global Respiratory Pathogen Surveillance Program. VE was estimated using a generalized linear mixed model with logit link and binomial distribution, adjusted for confounding effects. A total of 7114 adults including 2543 medically attended, laboratory-confirmed influenza-positive cases were identified. Using influenza test-negative controls, the adjusted VE in adults was 40% [95% confidence interval (CI): 33–46%] overall, including 46% (95% CI: 36–55%) for influenza A(H1N1)pdm09, 32% (95% CI: 19–42%) for influenza A(H3N2), and 54% (95% CI: 44–62%) for influenza B. The age-stratified analysis showed that VE estimates against influenza A(H1N1)pdm09 (34%; 95% CI: −29–66%) and influenza A(H3N2) (6%; 95% CI: −60–45%) were low and non-significant for elderly adults ≥65 years of age. Overall VE estimates against any influenza or by influenza (sub)types in adults were consistent when using influenza test-negative controls, non-influenza virus positive controls, and pan-negative controls. Inactivated influenza vaccination provided moderate protection against influenza virus infection, based on the analysis from a large number of adults aged ≥18 years over multiple influenza seasons.

scholarly journals Evaluation of the Antibacterial Effect of AH Plus Sealer Modified with Silver Nanoparticles

2021 ◽  
Author(s):  
Farzaneh Afkhami ◽  
Shifteh Nasri ◽  
Sara Valizadeh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Effect ◽  
Study Groups ◽  
Statistical Test ◽  
The Third ◽  
Significant Difference ◽  
Ah Plus ◽  
Bacterial Leakage ◽  
Negative Controls ◽  
Positive Controls

Abstract Background: The present study aimed to evaluate the antibacterial activity of AH Plus sealer modified with silver nanoparticles. Methods: Forty sound teeth were obturated using lateral compaction technique except for five teeth as the negative controls. After considering five teeth as the positive controls, the remaining teeth were assigned to two groups (n=15) in terms of the sealer used (AH Plus sealer and silver nanoparticle-modified AH Plus). Bacterial leakage was evaluated in saliva using the two-wall technique in every 24 hours. When all the samples in the test groups were contaminated, the study was terminated. The data were analyzed with log-rank statistical test.Results: The samples became contaminated with bacteria until the end of the third month in both groups. There was no significant difference in microleakage time between study groups (P>0.05).Conclusions: The incorporation of silver nanoparticles at concentrations tested into the AH Plus sealer did not prevent bacterial leakage.

scholarly journals Analysis of the sealing ability of different obturation techniques in oval-shaped canals: a study using a bacterial leakage model

2006 ◽  
Vol 20 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Gustavo De Deus ◽  
Cristiana Francescutti Murad ◽  
Claudia Mendonça Reis ◽  
Eduardo Gurgel-Filho ◽  
Tauby Coutinho Filho
Keyword(s):  
Human Saliva ◽  
Statistical Significant Difference ◽  
Leakage Model ◽  
Root Canals ◽  
Sealing Ability ◽  
Significant Difference ◽  
Bacterial Leakage ◽  
Negative Controls ◽  
Positive Controls

The aim of this study was to evaluate the sealing ability of 3 obturation techniques in oval canals using a bacterial leakage model. Seventy mandibular incisors with oval canals were selected after buccolingual and mesiodistal radiographs, and divided into three groups. After instrumentation, the canals were obturated respectively by lateral condensation (G1), warm vertical condensation (G2) and Thermafil (G3). Five root canals were not obturated and served as positive controls; five teeth with intact crowns served as negative controls. All teeth were mounted on a 2 chamber apparatus and then exposed to human saliva. The number of days required for the entire contamination of the root canals was recorded, observing the turbidity of the BHI broth, for a period of 100 days. 84.21% of the samples from the lateral condensation group (G1); 84.21% of the samples from the warm vertical condensation group (G2); and 89.47% of the samples from the Thermafil System group (G3) showed no contamination at the end of the study. No statistical significant difference was found among the three techniques. The quality of the apical seal in the three techniques tested was similar.

scholarly journals Nanocarriers of Miconazole or Fluconazole: Effects on Three-Species Candida Biofilms and Cytotoxic Effects In Vitro

2021 ◽  
Vol 7 (7) ◽  
pp. 500
Author(s):  
Anne Caroline Morais Caldeirão ◽  
Heitor Ceolin Araujo ◽  
Laís Salomão Arias ◽  
Wilmer Ramírez Carmona ◽  
Gustavo Porangaba Miranda ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Artificial Saliva ◽  
Cytotoxic Effects ◽  
Promising Alternative ◽  
Colony Forming Units ◽  
Mtt Reduction ◽  
Diphenyl Tetrazolium Bromide ◽  
Negative Controls ◽  
Positive Controls

The contribution of different Candida species in oral fungal infections has stimulated the search for more effective therapies. This study assessed the antibiofilm effects of nanocarriers of miconazole (MCZ) or fluconazole (FLZ) on Candida biofilms, and their cytotoxic effects on murine fibroblasts. Three-species biofilms (Candida albicans/Candida glabrata/Candida tropicalis) were formed on 96-well plates, and they were treated with nanocarriers (iron oxide nanoparticles coated with chitosan—“IONPs-CS”) of MCZ or FLZ at 39/78/156 µg/mL; antifungals alone at 156 µg/mL and artificial saliva were tested as positive and negative controls, respectively. Biofilms were analyzed by colony forming units (CFU), biomass, metabolic activity, and structure/viability. The cytotoxicity (L929 cells) of all treatments was determined via 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) reduction assay. Data were submitted to one- or two-way ANOVA, followed by Tukey’s or Fisher LSD’s tests (p < 0.05). IONPs-CS-MCZ at 78 µg/mL promoted similar antibiofilm and cytotoxic effects compared with MCZ at 156 µg/mL. In turn, IONPs-CS-FLZ at 156 µg/mL was overall the most effective FLZ antibiofilm treatment, surpassing the effects of FLZ alone; this nanocarrier was also less cytotoxic compared with FLZ alone. It can be concluded that both nanocarriers are more effective alternatives to fight Candida biofilms compared with their respective positive controls in vitro, being a promising alternative for the treatment of oral fungal infections.

Comparative Assessment of ActiV GP/Glass Ionomer Sealer, Resilon/Epiphany, and Gutta-Percha/AH Plus Obturation: A Bacterial Leakage Study

2008 ◽  
Vol 34 (6) ◽  
pp. 725-727 ◽  
Author(s):  
Joel N. Fransen ◽  
Jianing He ◽  
Gerald N. Glickman ◽  
Alejandro Rios ◽  
Jay D. Shulman ◽  
...  
Keyword(s):  
Comparative Assessment ◽  
Glass Ionomer ◽  
Gutta Percha ◽  
Ah Plus ◽  
Bacterial Leakage

Cardiovascular parameters in a mixed-sex swine study of severe decompression sickness treated with the emulsified perfluorocarbon Oxycyte

2015 ◽  
Vol 118 (1) ◽  
pp. 71-79 ◽  
Author(s):  
R. T. Mahon ◽  
W. A. Cronin ◽  
M. Bodo ◽  
S. Tirumala ◽  
D. P. Regis ◽  
...  
Keyword(s):  
Cardiac Output ◽  
Decompression Sickness ◽  
Mortality Rates ◽  
Rapid Onset ◽  
Systemic Arterial Pressure ◽  
Saline Control ◽  
Physiological Effects ◽  
Control Groups ◽  
And Control ◽  
Observation Period

Intravenous perfluorocarbons (PFC) have reduced the effects of decompression sickness (DCS) and improved mortality rates in animal models. However, concerns for the physiological effects of DCS combined with PFC therapy have not been examined in a balanced mixed-sex population. Thirty-two (16 male, 16 female) instrumented and sedated juvenile Yorkshire swine were exposed to 200 feet of seawater (fsw) for 31 min of hyperbaric air. Pulmonary artery pressure (PAP), cardiac output (CO), and systemic arterial pressure (SAP) were monitored before (control) and after exposure. Animals were randomized to treatment with Oxycyte (5 ml/kg; Oxygen Biotherapeutics, Inc., Morrisville, NC) vs. saline (control) with 100% oxygen administered upon DCS onset; animals were observed for 90 min. Parameters recorded and analyzed included PAP, CO, and SAP. In all animals PAP began to rise prior to cutis marmorata (CM) onset, the first sign of clinical DCS, generally peaking after CM onset. Female swine, compared with castrated males, had a more rapid onset of CM (7.30 vs. 11.46 min postsurfacing) and earlier onset to maximal PAP (6.41 vs. 9.69 min post-CM onset). Oxycyte therapy was associated with a sustained PAP elevation above controls in both sexes (33.41 vs. 25.78 mmHg). Significant pattern differences in PAP, CO, and SAP were noted between sexes and between therapeutic groups. There were no statistically significant differences in survival or paralysis between the PFC and control groups during the 48-h observation period. In conclusion, Oxycyte therapy for DCS is associated with a prolonged PAP increase in swine. These species and sex differences warrant further exploration.

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