scholarly journals Disturbance of Cell Proliferation in Response to Mobile Phone Frequency Radiation

2009 ◽  
Vol 60 (1) ◽  
pp. 109-115 ◽  
Author(s):  
Ivančica Trošić ◽  
Ivan Pavičić
Keyword(s):  
Cell Proliferation ◽  
Mobile Phone ◽  
Mitotic Index ◽  
Specific Absorption Rate ◽  
Continuous Wave ◽  
V79 Cells ◽  
Dividing Cells ◽  
Negative Controls ◽  
Frequency Radiation ◽  
Positive Controls

Disturbance of Cell Proliferation in Response to Mobile Phone Frequency RadiationThe aim of study was to determine the influence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79 cells after 1 h, 2 h, and 3 h of exposure. V79 cells were cultured in standard laboratory conditions and exposed to continuous-wave (CW) RF/MW radiation of 935 MHz, electric field strength of (8.2±0.3) V m-1, and specific absorption rate (SAR) of 0.12 W kg-1. To identify proliferation kinetics, the cells were counted for each hour of exposure 24 h, 48 h, 72 h, and 96 h after respective exposures. Microtubule proteins were determined using specific immunocytochemical methods. Cell smears were analysed under a fluorescent microscope. The study included negative and positive controls. Mitotic index was determined by estimating the number of dividing cells 24 h after exposure and dividing it with the total number of cells. In comparison to the controls, cell proliferation declined in cells exposed for three hours 72 h after irradiation (p<0.05). Microtubule structure was clearly altered immediately after three hours of irradiation (p<0.05). The mitotic index in RF/MW-exposed cells did not differ from negative controls. However, even if exposure did not affect the number of dividing cells, it may have slowed down cell division kinetics as a consequence of microtubule impairment immediately after exposure.

Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

Biologia ◽  
2006 ◽  
Vol 61 (3) ◽  
Author(s):  
Ivan Pavicic ◽  
Ivancica Trosic
Keyword(s):  
Cell Proliferation ◽  
Electromagnetic Field ◽  
Continuous Wave ◽  
Biological Effects ◽  
Chinese Hamster ◽  
Established Cell Line ◽  
V79 Cells ◽  
Trypan Blue Exclusion ◽  
Cell Counts ◽  
Established Cell

AbstractConsidering often contradictory data on biological effects of mobile phones frequencies on established cell culture lines, our study aimed at evaluating the influence of 864 MHz electromagnetic field on proliferation, colony forming ability and viability of Chinese hamster lung cells continuous line V79. Prior to exposure for 1, 2 and 3 hours in transversal electromagnetic mode cell (TEM-cell) equipped by Philips PM 5508 signal generator cell samples were sub-cultivated for one day. Cell samples were exposed to 864 MHz continuous wave at an average specific absorption rate (SAR) of 0.08 W/kg. To determine cell growth, V79 cells were plated in concentration of 1 × 104 cells per milliliter of nutrient medium RPMI 1640, and raised in a humified atmosphere at 37°C in 5% CO2. Cell proliferation was determined by cell counts for each hour of exposure on post-exposure day 1, 2, 3, 4 and 5. To identify colony-forming ability, cells were cultivated in concentration of 40 cells/mL of RPMI 1640 and incubated according to the deliberated experimental protocol. Colony forming ability for each hour of exposure was defined by colony counts on experimental day 7. Trypan blue exclusion test was used to determine viability of cells. In comparison to sham-exposed cells, growth curve of irradiated cell samples showed significant decrease (p < 0.05) after 2 and 3 hours of exposure on experimental day 3, respectively. Both, the colony forming ability and viability of irradiated cells did not significantly differ from exposed “mock” condition. Under strictly controlled laboratory conditions, applied radiofrequency microwaves (RF/MW) irradiation significantly affected cell proliferation kinetics but not viability or ability of V79 cells to form colonies. Sophisticated mechanism of action is intending to be elucidated in the further research which will include insight into the RF/MW related event at the subcellular level.

Microbial Leakage Evaluation Of Warm Gutta – Percha Techniques

2020 ◽  
Author(s):  
Antonio Libonati ◽  
Anna Piccinno ◽  
Gianni Gallusi ◽  
Edoardo Montemurro ◽  
Virginia Di Taranto ◽  
...  
Keyword(s):  
Continuous Wave ◽  
Control Groups ◽  
Gutta Percha ◽  
Bacterial Leakage ◽  
Negative Controls ◽  
Observation Period ◽  
Positive Controls

Abstract Background: To compare bacterial leakage of MicroHeat and continuous wave with and without endodontic sealer. Methods: Thirty – eight single – rooted extracted mandibular premolars were selected and randomly divided into four experimental groups (n=8) and two control groups (n=3). Teeth were prepared with Mtwo NiTi files and obturated with MicroHeat or System B with or without endodontic sealer. Three teeth were used as positive controls (Ct+) and three intact teeth served as negative controls (Ct-). All samples were tested for bacterial infiltration every day for 60 days. Results: On day 32 overall contamination value was 62,5% for Mseal, 75% for Mnoseal, 75% for SBseal and 37,8% for SBnoseal; after 60 days, the final contamination result was 100% for Mseal, Mnoseal and SBseal and 87,5% for SBnoseal. Conclusions: At the end of the observation period, groups showed no statistically significant differences.

Expression of β-Catenin in Hepatocellular Carcinoma

2001 ◽  
Vol 71 (3) ◽  
pp. 116-125
Author(s):  
Norina Basa ◽  
Daniela Lazar ◽  
Remus Cornea ◽  
Sorina Taban ◽  
Melania Ardelean ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Mitotic Index ◽  
Histological Grade ◽  
Proliferation Index ◽  
Tumor Cell Proliferation ◽  
Ki 67 ◽  
B Virus ◽  
Development And Evolution

Alteration of β-catenin expression is involved in the development and evolution of hepatocellular carcinoma (HCC); β-catenin is able to influence tumor cell proliferation. We analyzed the immunohistochemical (IHC) expression of β-catenin on a group of 32 patients diagnosed with HCC using the anti-β-catenin monoclonal antibody (clone E247). We correlated the expression of β-catenin with the proliferation index of Ki-67 (PI Ki-67), the mitotic index (MI) and other clinical and pathological features. We observed an altered β-catenin expression in 58.38% of all HCC cases. This expression was insignificantly correlated with tumor size (]5 cm) (p = 0.683), histological grade G1-G2 (p = 0.307), vascular invasion (p = 0.299) and advanced pT stage (p = 0.453); we obtained a significantly higher MI in HCC with altered β-catenin expression (p = 0.018), as compared to HCC without overexpression (1.66 � 1.37) (p = 0.038) and a PI Ki-67 of 22.49 � 20.1 and 28.24 � 18.2, respectively in tumors with altered β-catenin expression with insignificant differences compared to HCC without overexpression (25.95 � 15.2) (p = 0.682 and p = 0.731, respectively). According to the results we obtained, aberrant β-catenin expression in HCC was correlated with a high mitotic index, therefore playing an important role in tumor progression by stimulating tumor cell proliferation; non-nuclear β-catenin overexpression can have a pathological significance in HCC, especially in cases of HCC associated with hepatitis B virus (HBV) infection.

scholarly journals Nanocarriers of Miconazole or Fluconazole: Effects on Three-Species Candida Biofilms and Cytotoxic Effects In Vitro

2021 ◽  
Vol 7 (7) ◽  
pp. 500
Author(s):  
Anne Caroline Morais Caldeirão ◽  
Heitor Ceolin Araujo ◽  
Laís Salomão Arias ◽  
Wilmer Ramírez Carmona ◽  
Gustavo Porangaba Miranda ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Artificial Saliva ◽  
Cytotoxic Effects ◽  
Promising Alternative ◽  
Colony Forming Units ◽  
Mtt Reduction ◽  
Diphenyl Tetrazolium Bromide ◽  
Negative Controls ◽  
Positive Controls

The contribution of different Candida species in oral fungal infections has stimulated the search for more effective therapies. This study assessed the antibiofilm effects of nanocarriers of miconazole (MCZ) or fluconazole (FLZ) on Candida biofilms, and their cytotoxic effects on murine fibroblasts. Three-species biofilms (Candida albicans/Candida glabrata/Candida tropicalis) were formed on 96-well plates, and they were treated with nanocarriers (iron oxide nanoparticles coated with chitosan—“IONPs-CS”) of MCZ or FLZ at 39/78/156 µg/mL; antifungals alone at 156 µg/mL and artificial saliva were tested as positive and negative controls, respectively. Biofilms were analyzed by colony forming units (CFU), biomass, metabolic activity, and structure/viability. The cytotoxicity (L929 cells) of all treatments was determined via 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) reduction assay. Data were submitted to one- or two-way ANOVA, followed by Tukey’s or Fisher LSD’s tests (p < 0.05). IONPs-CS-MCZ at 78 µg/mL promoted similar antibiofilm and cytotoxic effects compared with MCZ at 156 µg/mL. In turn, IONPs-CS-FLZ at 156 µg/mL was overall the most effective FLZ antibiofilm treatment, surpassing the effects of FLZ alone; this nanocarrier was also less cytotoxic compared with FLZ alone. It can be concluded that both nanocarriers are more effective alternatives to fight Candida biofilms compared with their respective positive controls in vitro, being a promising alternative for the treatment of oral fungal infections.

Haemopoiesis in Lepidoptera. III. A note on the multiplication of spherule cells and granular haemocytes

1983 ◽  
Vol 61 (1) ◽  
pp. 275-277 ◽  
Author(s):  
J. W. Arnold ◽  
C. F. Hinks
Keyword(s):  
Mitotic Index ◽  
Alcian Blue ◽  
Maximum Production ◽  
Spherule Cell ◽  
Dividing Cells ◽  
Lepidoptera Noctuidae ◽  
Potential Maximum ◽  
Hematoxylin Eosin ◽  
Cell Mitosis

Blood films from early sixth instar larvae of Euxoa declarata (Lepidoptera: Noctuidae) stained in hematoxylin – eosin – alcian blue showed unequivocal examples of mitosis in spherule cells. The improved visibility of mitosis and the estimation of the mitotic index from counts of dividing cells per 1000 cells of each type indicated a far greater potential maximum production of spherule cells and granular haemocytes by mitosis than reported previously. Certain other methods of staining showed similar clear examples of spherule cell mitosis.

JEJUNAL BIOPSIES IN INFANT MALNUTRITION: WITH SPECIAL REFERENCE TO MITOTIC INDEX

PEDIATRICS ◽  
1966 ◽  
Vol 38 (4) ◽  
pp. 605-612
Author(s):  
Oscar Brunser ◽  
Alejandro Reid ◽  
Fernando Mönckeberg ◽  
Alejandro Maccioni ◽  
Iván Contreras
Keyword(s):  
Cell Proliferation ◽  
Celiac Disease ◽  
Mitotic Index ◽  
Energy Supply ◽  
Caloric Intake ◽  
Jejunal Mucosa ◽  
Common Pattern ◽  
Hormonal Mechanism ◽  
Histological Aspect ◽  
Normal Controls

The histological aspect and the mitotic index of jejunal biopsies were studied in 8 normal infants, in 18 marasmic infants, and in 10 infants with kwashiorkor. The mucosa of the marasmic infants was similar to the normal controls, although thinner. In kwashiorkor the mucosa was similar to celiac disease. In marasmic infants fed on the same diet, the mitotic index is significantly lower in infants who do not show weight increase when compared with those who do increase in weight. In kwashiorkor the mitotic index is closer to, although also significatively lower than, normal. It is suggested that these differences are due to the low caloric intake in marasmus where the energy supply is not sufficient to maintain the rate of cell proliferation; a hormonal mechanism governed by the pituitary gland is also suggested. In kwashiorkor the histological aspect similar to celiac disease seems to indicate a common pattern of reaction of the jejunal mucosa due to different causes.

scholarly journals "In vitro" evaluation of the apical sealing of root canals obturated with different techniques

2003 ◽  
Vol 11 (3) ◽  
pp. 181-185 ◽  
Author(s):  
Viviane Haiub Brosco ◽  
Norberti Bernardineli ◽  
Ivaldo Gomes de Moraes
Keyword(s):  
Continuous Wave ◽  
The Other ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Root Canals ◽  
Gutta Percha ◽  
Sealing Ability ◽  
Better Than ◽  
Positive Controls

The purpose of this study was to compare the apical sealing of root canals obturated with different techniques. One hundred-six human mandibular incisors were submitted to instrumentation by means of the step-back technique. After instrumentation, one hundred teeth received an impermeable coating on the external surfaces of the crown and root (except for the area nearby the apical foramen). Afterwards, they were divided in five groups containing twenty elements each, according to the obturation technique employed: 1. lateral condensation with Kerr file; 2. continuous wave of condensation technique with System B; 3. thermoplasticized injectable gutta-percha technique with the Ultrafil system; 4. mechanically thermoplasticized gutta-percha with the JS Quick-Fill system and 5. thermoplasticized gutta-percha associated to a master cone with the Microseal system. The six remaining teeth were employed as negative and positive controls. After obturation, the access cavities were sealed and the teeth were immersed in aqueous 2% methylene blue dye for 72 hours at 37ºC. After that, the teeth were longitudinally sectioned and the apical microleakage was evaluated in a stereomicroscope. The Microseal system presented the best apical sealing ability, followed by System B, JS Quick-Fill, Ultrafil and the lateral condensation technique. The statistical analysis of the results demonstrated that: 1. the Microseal system presented an apical sealing similar to System B and better than the other groups; 2. System B presented better apical sealing than the lateral condensation technique, being similar to the other groups; and 3. the lateral condensation, Ultrafil and JS Quick-Fill groups demonstrated similar sealing ability.

scholarly journals 5-AMINOURACIL TREATMENT

1971 ◽  
Vol 48 (2) ◽  
pp. 248-252 ◽  
Author(s):  
S. H. Socher ◽  
D. Davidson
Keyword(s):  
Mitotic Index ◽  
Vicia Faba ◽  
Cell Population ◽  
Lateral Roots ◽  
Proliferating Cells ◽  
Dividing Cells ◽  
Proliferating Cell ◽  
Two Populations

Treatment of Vicia faba lateral roots with a range of concentrations of 5-aminouracil (5-AU) indicate that cells are stopped at a particular point in interphase. The timing of the fall in mitotic index suggests that cells are held at the S - G2 transition. When cells are held at this point, treatments with 5-AU can be used to estimate the duration of G2 + mitosis/2 of proliferating cells. Treatment with 5-AU can also be used to demonstrate the presence of subpopulations of dividing cells that differ in their G2 duration. Using this method, 5-AU-induced inhibition, we have confirmed that in V. faba lateral roots there are two populations of dividing cells: (a) a fast-dividing population, which makes up ∼85% of the proliferating cell population and has a G2 + mitosis/2 duration of 3.3 hr, and (b) a slow-dividing population, which makes up ∼15% of dividing cells and has a G2 duration in excess of 12 hr. These estimates are similar to those obtained from percentage labeled mitosis (PLM) curves after incorporation of thymidine-3H.

A Rapid Degradation of Calponin 2 Is Required for Cytokinesis

2021 ◽  
Author(s):  
Airong Qian ◽  
Tzu-Bou Hsieh ◽  
M. Moazzem Hossain ◽  
Jim J.-C. Lin ◽  
J.-P. Jin
Keyword(s):  
Cell Proliferation ◽  
Cell Division ◽  
Underlying Mechanism ◽  
Fluorescent Imaging ◽  
Hek293 Cells ◽  
Computer Assisted ◽  
Over Expression ◽  
Dividing Cells ◽  
Ubiquitin Proteasome ◽  
Dynamic Image Analysis

Calponin 2 is an actin cytoskeleton-associated protein and plays a role in regulating cell motility-related functions such as phagocytosis, migration and division. We previously reported that the expression of calponin 2 inhibits the rate of cell proliferation. To investigate the underlying mechanism, our present study found that the levels of endogenous calponin 2 in NIH3T3 and HEK293 cells rapidly decreased prior to cell division characterized by an absence at the actin contractile ring. In cells lacking endogenous calponin 2, transfective expression of GFP-fusion calponin 2 inhibited cell proliferation similar to that of non-fusion calponin 2. Fluorescent imaging studies of mitotic cells indicated that a proper level of calponin 2 expression and effective degradation during cytokinesis are necessary for normal cell division. Computer-assisted dynamic image analysis of dividing cells revealed that over-expression of calponin 2 significantly affects motile and shape behaviors of cells only on the interval from the start of anaphase to the start of cytokinesis, i.e., the pre-cytokinesis phase, but not on the interval from the start of cytokinesis to 50% completion of cytokinesis. The pre-cytokinesis degradation of calponin 2 was attenuated by MG132 inhibition of the ubiquitin proteasome and inhibitor of protein kinase C (PKC), suggesting that PKC phosphorylation-triggered degradation of calponin 2 could determine the rate of cytokinesis. The novel role of calponin 2 in regulating the rate of cytokinesis may be targeted for therapeutic applications such as in an inhibition of malignant tumor growth.

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