scholarly journals Evaluation of the Antibacterial Effect of AH Plus Sealer Modified with Silver Nanoparticles

2021 ◽  
Author(s):  
Farzaneh Afkhami ◽  
Shifteh Nasri ◽  
Sara Valizadeh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Effect ◽  
Study Groups ◽  
Statistical Test ◽  
The Third ◽  
Significant Difference ◽  
Ah Plus ◽  
Bacterial Leakage ◽  
Negative Controls ◽  
Positive Controls

Abstract Background: The present study aimed to evaluate the antibacterial activity of AH Plus sealer modified with silver nanoparticles. Methods: Forty sound teeth were obturated using lateral compaction technique except for five teeth as the negative controls. After considering five teeth as the positive controls, the remaining teeth were assigned to two groups (n=15) in terms of the sealer used (AH Plus sealer and silver nanoparticle-modified AH Plus). Bacterial leakage was evaluated in saliva using the two-wall technique in every 24 hours. When all the samples in the test groups were contaminated, the study was terminated. The data were analyzed with log-rank statistical test.Results: The samples became contaminated with bacteria until the end of the third month in both groups. There was no significant difference in microleakage time between study groups (P>0.05).Conclusions: The incorporation of silver nanoparticles at concentrations tested into the AH Plus sealer did not prevent bacterial leakage.

scholarly journals Bacterial leakage assessment in root canals sealed with AH Plus sealer modified with silver nanoparticles

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Farzaneh Afkhami ◽  
Shifteh Nasri ◽  
Sara Valizadeh
Keyword(s):  
Silver Nanoparticles ◽  
Root Canal ◽  
Study Groups ◽  
Root Canal Filling ◽  
Root Canals ◽  
Leakage Resistance ◽  
Significant Difference ◽  
Ah Plus ◽  
Bacterial Leakage ◽  
Negative Controls

Abstract Background Successful endodontic therapy requires prevention of bacterial leakage between the root canal filing and root-canal walls. Sealing quality of a root canal filling depends strongly on the sealing ability of the sealer used. The present study aimed to evaluate the bacterial leakage resistance of AH Plus sealer modified with silver nanoparticles. Methods Forty sound teeth were obturated using lateral compaction technique except for five teeth as the negative controls. After considering five teeth as the positive controls (filled without sealer), the remaining teeth were assigned to two groups (n = 15) in terms of the sealer used (AH Plus sealer and silver nanoparticle-modified AH Plus). Bacterial leakage was evaluated in saliva using the two-chamber technique in every 24 h. When all the samples in the test groups were contaminated, the study was terminated. The data were analyzed with log-rank statistical test. Results All samples in both experimental groups were contaminated during the 3-months period of observation. There was no significant difference in contamination time between study groups (P > 0.05). Conclusions Silver nanoparticles used in tested concentration did not improve the bacterial leakage resistance of AH Plus sealer.

scholarly journals Improved sealing ability promoted by calcium silicate-based root canal sealers

2018 ◽  
Vol 17 ◽  
pp. 1-8
Author(s):  
Tatiana Vieira de Melo ◽  
Marina Carvalho Prado ◽  
Raphael Hirata Junior ◽  
Sandra Rivera Fidel ◽  
Emmanuel João Nogueira Leal da Silva ◽  
...  
Keyword(s):  
Root Canal ◽  
Calcium Silicate ◽  
Rank Test ◽  
Survival Curves ◽  
Sealing Ability ◽  
Significant Difference ◽  
Ah Plus ◽  
Negative Controls ◽  
Root Canal Sealers ◽  
Positive Controls

Aim: This study investigated the sealing ability produced by AH Plus (Dentsply DeTrey, Konstaz, Germany), EndoSequence BC sealer (Brasseler USA, Savannah, GA, USA), GuttaFlow (Coltène/Whaledent, Altstätten, France) and MTA Fillapex (Angelus, Londrina, Brazil). Methdos: A total of forty-six single-root human canines were prepared and randomly divided into four experimental groups (n=10): MTA Fillapex, EndoSequence BC Sealer, AH Plus or GuttaFlow. Teeth with intact crowns served as negative controls (n=3) and teeth filled with only gutta-percha served as positive controls (n=3). Teeth were mounted in a two-chamber apparatus and exposed to Enterococcus faecalis. The number of days over a 60-days period was recorded for the appearance of turbidity in the lower chamber. Kaplan-Meier method was used to estimate the survival curves. The nonparametric log-rank test was used to compare the survival curves using a significance level of 0.05. Results: The results at the end of the observation time were further analyzed by chi-square testing. All positive controls leaked within 24 h, whereas none of the negative controls leaked after 60 days. EndoSequence BC Sealer and MTA Fillapex had significant less bacterial leakage (P<0.05) than the other tested sealers. No significant difference between AH Plus and GuttaFlow was observed (P>0.05). Conclusion: In conclusion, calcium-silicate based root canal sealers promoted improved sealing ability when compared to other endodontic sealers.

scholarly journals Analysis of the sealing ability of different obturation techniques in oval-shaped canals: a study using a bacterial leakage model

2006 ◽  
Vol 20 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Gustavo De Deus ◽  
Cristiana Francescutti Murad ◽  
Claudia Mendonça Reis ◽  
Eduardo Gurgel-Filho ◽  
Tauby Coutinho Filho
Keyword(s):  
Human Saliva ◽  
Statistical Significant Difference ◽  
Leakage Model ◽  
Root Canals ◽  
Sealing Ability ◽  
Significant Difference ◽  
Bacterial Leakage ◽  
Negative Controls ◽  
Positive Controls

The aim of this study was to evaluate the sealing ability of 3 obturation techniques in oval canals using a bacterial leakage model. Seventy mandibular incisors with oval canals were selected after buccolingual and mesiodistal radiographs, and divided into three groups. After instrumentation, the canals were obturated respectively by lateral condensation (G1), warm vertical condensation (G2) and Thermafil (G3). Five root canals were not obturated and served as positive controls; five teeth with intact crowns served as negative controls. All teeth were mounted on a 2 chamber apparatus and then exposed to human saliva. The number of days required for the entire contamination of the root canals was recorded, observing the turbidity of the BHI broth, for a period of 100 days. 84.21% of the samples from the lateral condensation group (G1); 84.21% of the samples from the warm vertical condensation group (G2); and 89.47% of the samples from the Thermafil System group (G3) showed no contamination at the end of the study. No statistical significant difference was found among the three techniques. The quality of the apical seal in the three techniques tested was similar.

scholarly journals Efficacy of intrathecal midazolam on enhancing analgesic effect of fentanyl in patients undergoing lower limb surgery

2018 ◽  
Vol 5 (10) ◽  
pp. 2726-2732
Author(s):  
Masoum Khoshfetrat ◽  
Sima Davarpanah ◽  
Aliakbar Keykha
Keyword(s):  
Nerve Block ◽  
Lower Limb ◽  
Analgesic Effect ◽  
Synergistic Effects ◽  
Sensory Nerve ◽  
Study Groups ◽  
The Third ◽  
Significant Difference ◽  
Lower Limb Surgery ◽  
The Mean

Background: Co-administration of drugs with synergistic effects is considered as one of the methods to increase the effectiveness of intrathecal anesthesia and to reduce the need for injectable analgesics. The purpose of this study was to investigate the efficacy of intrathecal midazolam on enhancing the analgesic effect of fentanyl in patients undergoing lower limb surgery. Materials: The present double-blinded clinical trial was conducted on 90 candidate patients undergoing lower limb orthopedic surgery in 2017 at Khatam-Al-Anbiya Hospital in the city of Zahedan, Iran. To this end, the patients were selected via convenience sampling method and then randomly divided into three groups. Afterwards, 3 cc of 0.5% hyperbaric Marcaine was injected intrathecally in the first group, 3 cc of Marcaine + 25 ug of fentanyl was administered to the second group, and 3 cc of Marcaine + 25 ug of fentanyl + 1 mg of midazolam was administered to the third group (the final volume of all three syringes was brought up to 3.7 cc with normal saline). The duration of sensory nerve block and those of motor block and analgesia, as well as changes in vital signs, were then measured in these groups. Moreover, the data were analyzed using SPSS Statistics (Version 22.0) through Chi-square test and one-way analysis of variance (ANOVA). Results: The mean age and gender distribution of the patients in the three study groups did not differ significantly. The mean duration of sensory nerve block was equal to 98.6+/-11.1 min in the first group, 142.2+/-12.4 min in the second group, and 174.3+/-10.9 min in the third group; all showed a statistically significant difference (P=0.0001, P=0.001, P=0.000).The mean duration of analgesia was also reported to be 204.43+/-0.3 min in the first group, 323.62+/-7.4 min in the second group, and 526.22+/-0.9 min in the third group, indicating a statistically significant difference between the three study groups (P=0.0001, P=0.000, P=000). Conclusion: The results of this study demonstrated that adding midazolam to intrathecal fentanyl could significantly increase the duration of sensory nerve block and that of post-operative analgesia.  

scholarly journals Antibacterial Effect Of Kaempferia Galanga L Extract On Lactobacillus Acidophilus –In Vitro

2017 ◽  
Vol 1 (01) ◽  
pp. 22
Author(s):  
Josi Saraswati ◽  
Annisa Septalita ◽  
Arini Bovita. N
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Acidophilus ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Inhibition Effect ◽  
Kaempferia Galanga ◽  
Significant Difference ◽  
Agar Media ◽  
Negative Controls

Introduction: Lactobacillus acidophilus is one of the bacteria causes dental caries. The previous study has shown that Kaempferia galanga extract has a potential to inhibit the growth of Lactobacillus acidophilus.Objective: To determine the antibacterial effect of Kaempferia galanga extract to Lactobacillus acidophilus.Methods:Kaempferia galanga is extracted in 3 different solvents:dichlormethane, ethanol, and aquades. For each solvent, 0.2 μl Kaempferia galanga extractdroped into 6 mm steril paper dics. 0.1 ml Lactobacillus acidophilus inoculated on MRS agar. Each disc contains extract were impragnated into the agar media, then incubated at 370C for 24 hours, and inhibition zone measured.Results: Mean scores of Kaempferia galanga extract in 3 different solvents are: Kaempferia galanga (dichlormethane) is 1.6400; Kaempferia galanga (ethanol) is 1.7440; Kaempferia galanga extract is 1.6600; boiled Kaempferia galanga is 1.7000. Using Mann-Whitney Test, the results are: negative controls have no inhibition effect on Lactobacillus acidophilus compaired to Kaempferia galanga extract, comparation of those 4 Kaempferia galangal treatments shows no significant difference, those 4 Kaempferia galanga treatments compaired to erythromycin antibacterial effect shows significant difference, otherwise 4 Kaempferia galanga treatments compaired to penicillin shows no significant difference except Kaempferia galanga (ethanol).Conclusions: Kaempferia galanga extract can kill Lactobacillus acidophilus. Inhibition effect of Kaempferia galanga extract has no significant difference to penicillin but lower inhibition effect than erythromycin. The Kaempferia galanga extracts showed better antibacterial activity than penicillin.

scholarly journals Comparing the effects of three different doses of caudal ketamine plus bupivacaine on pain control after paediatric surgery

2018 ◽  
Vol 5 (8) ◽  
pp. 2572-2580
Author(s):  
Masoum Khoshfetrat ◽  
Reza Davoodi ◽  
Aliakbar Keykha
Keyword(s):  
Pain Intensity ◽  
Pain Control ◽  
Pediatric Surgery ◽  
Study Groups ◽  
Paediatric Surgery ◽  
The Third ◽  
Physical Status Classification ◽  
Caudal Anaesthesia ◽  
Significant Difference ◽  
Different Doses

Introduction: Adding ketamine to bupivacaine in caudal anaesthesia is likely to increase its analgesic effect. However, it is not clear which dose of ketamine will have the greatest impact and the lowest level of complications. Thus, the purpose of this study was to compare the effects of three different doses of caudal ketamine plus bupivacaine on pain control after pediatric surgery. Methodology: The present double-blinded clinical trial was conducted on 69 pediatric patients, of age ranging from 6 months to 10 years. Patients were assessed via the American Society of Anaesthesiologists (ASA) physical status classification system (ASA I-II), and had been also hospitalized for herniorrhaphy and orchidopexy. The patients were randomly divided into three groups. The first group received 0.75 mg/kg of 0.25% bupivacaine plus 0.25 mg/kg of ketamine, the second group received 0.75 mg/kg of 0.25% bupivacaine and 0.5 mg/kg of ketamine, and the third group received 0.75 mg/kg of 0.25% bupivacaine with 0.75 mg/kg of ketamine (as caudal anaesthesia). The duration of motor block and analgesia, as well as sedation levels, were measured for each study group. Each patient’s level of pain was also measured (at 1, 2, 3, 4, 6 and 24 hours after the surgery) via the All India Institute of Medical Sciences (AIIMS) pain discomfort scale. The data were then compared using SPSS Statistics (Version 22), with descriptive statistics, analysis of variance (ANOVA), and Chi-square test. Results: The findings revealed that gender distribution did not differ significantly in the three study groups (p=0.896). The mean age of the first, second, and third group were reported to be 2.82±1.86, 3.1±2.4, and 2.48±1.06, respectively; thus, there was no significant difference in terms of age between the three groups (p=0.569). Upon examining the AIIMS pain discomfort scale scores during the first 24 hours following surgery, it was observed that the pain intensity was higher in the first group than in the second and third groups. Moreover, evaluation of the pain intensity in the first and the second groups revealed comparable results, while those of the second and the third groups did not show a significant difference in this respect. Conclusion: The results demonstrated that the most effective and safest dose of ketamine, in this study, for pain control after pediatric surgery in the inguinal region was the 0.5 mg/kg dose.

scholarly journals Antibacterial Properties of Aloe vera on Intracanal Medicaments against Enterococcus faecalis Biofilm at Different Stages of Development

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Negin Ghasemi ◽  
Mahsa Behnezhad ◽  
Mohammad Asgharzadeh ◽  
Elham Zeinalzadeh ◽  
Hossein Samadi Kafil
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Study Groups ◽  
Single Root ◽  
Significant Difference ◽  
Planktonic Form ◽  
Phosphate Buffered Saline

Background. Use of herbal compounds as an intracanal medicament in the field of endodontics has become noteworthy, one of which is the Aloe vera compound whose antibacterial effect has already been proven in the planktonic form of Enterococcus faecalis. The purpose of this study is to evaluate the antibacterial effect of Aloe vera on E. faecalis biofilms at the 4th and 6th week of development. Materials and Methods. 130 single root canal teeth without anomalies and caries were used. They were divided into two groups of 65 teeth for four and six weeks of biofilm production. Five samples of each group were examined for confirmation of biofilm formation under an electron microscope. Study groups were investigated with an antimicrobial agent as an intracanal medicament including 20 samples treated with Aloe vera, calcium hydroxide, and phosphate-buffered saline, and biofilm and survival of pathogens were investigated. Dentin chip suspensions were used for colony-forming unit (CFU) counting to estimate remaining E. faecalis counts. Results. The CFU mean in the 4th week subgroup in Aloe vera, phosphate-buffered saline, and calcium hydroxide was 0, 69166.66 ± 31688.58, and 25000 ± 30822.07, and in the 6th week, it was 136.36 ± 323.33, 95000 ± 12247.44, and 27501.66 ± 36570.34, respectively, which showed a significant difference between the used materials ( p < 0.05 ). Conclusion. Aloe vera, in contrast to calcium hydroxide, eliminated 4th and 6th week biofilms and showed remarkable antibacterial properties against E. faecalis biofilm. These results support potency of Aloe vera to use as a natural antimicrobial material in the intracanal medicament.

Microbial Leakage Evaluation Of Warm Gutta – Percha Techniques

2020 ◽  
Author(s):  
Antonio Libonati ◽  
Anna Piccinno ◽  
Gianni Gallusi ◽  
Edoardo Montemurro ◽  
Virginia Di Taranto ◽  
...  
Keyword(s):  
Continuous Wave ◽  
Control Groups ◽  
Gutta Percha ◽  
Bacterial Leakage ◽  
Negative Controls ◽  
Observation Period ◽  
Positive Controls

Abstract Background: To compare bacterial leakage of MicroHeat and continuous wave with and without endodontic sealer. Methods: Thirty – eight single – rooted extracted mandibular premolars were selected and randomly divided into four experimental groups (n=8) and two control groups (n=3). Teeth were prepared with Mtwo NiTi files and obturated with MicroHeat or System B with or without endodontic sealer. Three teeth were used as positive controls (Ct+) and three intact teeth served as negative controls (Ct-). All samples were tested for bacterial infiltration every day for 60 days. Results: On day 32 overall contamination value was 62,5% for Mseal, 75% for Mnoseal, 75% for SBseal and 37,8% for SBnoseal; after 60 days, the final contamination result was 100% for Mseal, Mnoseal and SBseal and 87,5% for SBnoseal. Conclusions: At the end of the observation period, groups showed no statistically significant differences.

scholarly journals ANTITHROMBOTIC EFFECT OF TRIGONELLA FOENUM-GRACEUM ON COLLAGEN/ EPINEPHRINE-INDUCED THROMBOEMBOLISM IN MICE

2018 ◽  
Vol 10 (1) ◽  
pp. 56 ◽  
Author(s):  
Fadlina Chany Saputri ◽  
Astari Rachma Nityasa
Keyword(s):  
Survival Rate ◽  
Carboxymethyl Cellulose ◽  
Survival Rates ◽  
Study Groups ◽  
Male Mice ◽  
Antithrombotic Effect ◽  
Antithrombotic Activity ◽  
Tail Vein ◽  
Negative Controls ◽  
Positive Controls

Objective: This study aimed to further investigate the antiplatelet and antithrombotic activities of Trigonella foenum-graceum (TFG) in vivo.Methods: Male mice were divided into two experimental groups (bleeding duration and survival rate). The study groups comprised vehicle controls(administered carboxymethyl cellulose [CMC]), negative controls (administered CMC), positive controls (administered aspirin), and experimentaltreatment groups (administered TFG extract at three doses). Bleeding duration was assessed by excising the tail vein. Survival rate was determinedby inducing thrombosis through intravenous collagen/epinephrine administration.Results: In mice treated with TFG extract for 7 days, bleeding duration was significantly increased compared with that in controls (p<0.05). Moreover,mice treated with TFG showed increased survival rates compared with negative controls.Conclusion: TFG extract showed antithrombotic activity in mice by significantly increasing bleeding duration and survival rate.

scholarly journals Kadar Sianida Singkong Rebus dan Singkong Goreng

2016 ◽  
Vol 2 (2) ◽  
pp. 46
Author(s):  
Yeni Purwati ◽  
Anny Thuraidah ◽  
Dinna Rakhmina
Keyword(s):  
Statistical Test ◽  
Respiratory Enzymes ◽  
Titration Method ◽  
Toxic Compound ◽  
The Third ◽  
Cassava Leaves ◽  
Frying Process ◽  
Cassava Root ◽  
Significant Difference ◽  
Boiling Process

<p style="text-align: justify;">Cassava is the third staple food often used by people to make a variety of products. Carbohydrates from cassava also contains toxic compound such as cyanide which can inhibit the action of respiratory enzymes. Cyanide in cassava can be removed by boiling or frying process. The aim of research was to find out the cyanide level differences between boiled cassava and fried cassava. This type of research was experiment, with design of comparative studies approach. Cyanide levels were examined in 10 samples with 2 treatments using Argentometry Volhard titration method. The result showed the average of cyanide levels in boiled cassava was 0.772 ppm and in fried cassava was 1.069 ppm. The percentage of cyanide levels reduction in boiled cassava was 28,78%, while for the fried cassava was 0%. The statistical test obtained the value of p <0.05, that showed there was significant difference of cyanide level between boiled cassava and fried cassava. Based on these results, it is advisable to choose the boiling process for cassava consumption. Next research can be conducted on comparative levels of cyanide in the cassava root and cassava leaves.

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