scholarly journals Transcriptome-wide Development and Utilization of Novel Intron-length Polymorphic (ILP) Markers in Common Vetch (Vicia Sativa Subsp. Sativa)

2021 ◽  
Author(s):  
Xingyi Wei ◽  
Qiuxia Wang ◽  
Yitong Ma ◽  
Wenxian Liu
Keyword(s):  
Scale Development ◽  
Large Scale ◽  
Polymorphic Information Content ◽  
Intron Length ◽  
Germplasm Evaluation ◽  
Comparative Genomic ◽  
Vicia Sativa ◽  
Length Variation ◽  
Common Vetch ◽  
Leguminous Species

Abstract Common vetch (Vicia sativa subsp. sativa) is one of the most economically important forage legumes with rich nutritional value and multiple uses. Though the large-scale development and application of microsatellite markers have been conducted in common vetch germplasm evaluation, the investigation and exploitation of intron-length polymorphic (ILP) markers have not been systematically analyzed. In this study, the intron positions of common vetch genome were located by aligning the RNA-Seq sequences of common vetch with Medicago truncatula, soybean (Glycine max), and Arabidopsis thaliana genomic sequences, and used for VsILP marker development. A total of 10,400 markers were generated from 44,582 common vetch unigenes. Out of 300 randomly selected VsILP markers, 283 resulted in successful amplification in common vetch. Among these markers, 40 produced length variation in 30 common vetch accessions, collectively yielding 166 alleles with an average of 4.0 alleles per locus. The polymorphic information content (PIC) values extended from 0.06 to 0.81 with a mean of 0.49. Of the 283 VsILP markers, 84.8% exhibited transferability to leguminous and non-leguminous species. We presented here the first large-scale development of ILP markers in common vetch and their utility in germplasm evaluation and transferability, which will be valuable for further comparative genomic studies, genetic relationship assessments, and marker-assisted breeding of leguminous and non-leguminous species.

Scanning electron microscopy observations of pollen morphology in common vetch (Vicia sativa) subspecies

2021 ◽  
pp. 1-14
Author(s):  
Rui Dong ◽  
Zhongjie Lu ◽  
Zhengyu Yang ◽  
Yanrong Wang ◽  
Chao Chen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Polar View ◽  
Polar Axis ◽  
Vicia Sativa ◽  
Common Vetch ◽  
Plant Phylogeny ◽  
Axis Length ◽  
Scanning Electron

Abstract Common vetch (Vicia sativa) is an important forage and green manure crop that is widely cultivated around the world. However, the large number of subspecies and accessions of common vetch has made taxonomic research on this species difficult. Pollen morphology data can provide important evidence in the study of plant phylogeny. Therefore, in this research, light microscopy and scanning electron microscopy were used to observe seven morphological traits of pollens from 22 common vetch accessions, and residual maximum likelihood and pattern analysis was conducted. The results showed that the pollen grains of the 22 accessions were all monad pollen and the polar view revealed three-lobed circular and triangular shapes, while the equatorial view mainly revealed an oblate shape; only one accession showed an oblate spherical shape. All accessions were 3-zonocolporate and the colpus length extended close to the poles. The polar axis length was (19.39 ± 0.97)–(42.12 ± 0.76) μm and the equatorial axis length was (35.97 ± 1.27)–(45.25 ± 0.81) μm. We found that qualitative traits of pollen shape, aperture polar view and ornamentation were highly stable. Among them, polar axis length, equatorial axis length and colpus length and width had significant differences among the accessions. The ratios of the equatorial and polar axes had significant differences among the subspecies. This trait information could be used for the classification and identification of common vetch accessions and subspecies.

scholarly journals Search for Resistant Genotypes to Cuscuta campestris Infection in Two Legume Species, Vicia sativa and Vicia ervilia

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 738
Author(s):  
Eva María Córdoba ◽  
Mónica Fernández-Aparicio ◽  
Clara Isabel González-Verdejo ◽  
Carmela López-Grau ◽  
María del Valle Muñoz-Muñoz ◽  
...  
Keyword(s):  
Agricultural Production ◽  
Host Plants ◽  
Resistance Breeding ◽  
Parasitic Plants ◽  
Vicia Sativa ◽  
Common Vetch ◽  
Germplasm Collections ◽  
Bitter Vetch ◽  
Cuscuta Campestris ◽  
Resistant Genotypes

The dodders (Cuscuta spp.) are parasitic plants that feed on the stems of their host plants. Cuscuta campestris is one of the most damaging parasitic plants for the worldwide agricultural production of broad-leaved crops. Its control is limited or non-existent, therefore resistance breeding is the best alternative both economically and environmentally. Common vetch (Vicia sativa) and bitter vetch (Vicia ervilia) are highly susceptible to C. campestris, but no resistant genotypes have been identified. Thus, the aim of this study was to identify in V. sativa and V.ervilia germplasm collections genotypes resistant to C. campestris infection for use in combating this parasitic plant. Three greenhouse screening were conducted to: (1) identify resistant responses in a collection of 154 accessions of bitter vetch and a collection of 135 accessions of common vetch genotypes against infection of C. campestris; (2) confirm the resistant response identified in common vetch accessions; and (3) characterize the effect of C. campestris infection on biomass of V. sativa resistant and susceptible accessions. Most common vetch and bitter vetch genotypes tested were susceptible to C. campestris. However, the V. sativa genotype Vs.1 exhibited high resistance. The resistant phenotype was characterized by a delay in the development of C. campestris posthaustorial growth and a darkening resembling a hypersensitive-like response at the penetration site. The resistant mechanism was effective in limiting the growth of C. campestris as the ratio of parasite/host shoot dry biomass was more significantly reduced than the rest of the accessions. To the best or our knowledge, this is the first identification of Cuscuta resistance in V. sativa genotypes.

When the Complexity of the Large-Scale Development Project Becomes a Roadblock to Access to Justice

2021 ◽  
pp. 1-8
Author(s):  
Pillkyu HWANG ◽  
Yae-Ahn PARK
Keyword(s):  
Scale Development ◽  
Large Scale ◽  
Development Project ◽  
State Enterprise ◽  
Access To Justice ◽  
South Korean ◽  
Western Power ◽  
Power Company ◽  
Hydropower Dam ◽  
To Come

On 23 July 2018, when the villagers gathered around the porch to wrap up the day with a good chat, one of the five auxiliary dams of the Xe-Pian Xe-Namnoy hydropower dam in Attapeu province, the southeastern state of Laos, collapsed. Four days before the collapse, reports of cracks and subsidence started to come through. It should have been enough to prompt evacuation warning issuance by the Xe-Pian Xe-Namnoy Power Co. Ltd (PNPC), a consortium of South Korean companies SK Engineering and Construction (SK E&C) and Korea Western Power Company (KOWEPO), Thailand-based RATCH Group, and Lao Holding State Enterprise (LHSE). PNPC has a Concession Agreement with the Laos government ‘to plan, design, finance, construct, own, operate and maintain’ the Xe-Pian Xe-Namnoy hydropower dam. The warning was issued, but it came too late.

Rhizobium changzhiense sp. nov., isolated from effective nodules of Vicia sativa L. in North China

2021 ◽  
Author(s):  
Junjie Zhang ◽  
Shanshan Peng ◽  
Mitchell Andrews ◽  
Chunzeng Liu ◽  
Yimin Shang ◽  
...  
Keyword(s):  
Type Species ◽  
Rrna Gene ◽  
Vicia Sativa ◽  
Common Vetch ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Rhizobial Species ◽  
Distinct Lineage ◽  
Type Strains

Three fast-growing rhizobial strains isolated from effective nodules of common vetch (Vicia sativa L.) were characterized using a polyphasic approach. All three strains were assigned to the genus Rhizobium on the basis of the results of 16S rRNA gene sequence analysis. Phylogenetic analysis based on concatenated atpD-recA genes separated the strains into a distinct lineage represented by WYCCWR 11279T, which showed average nucleotide identity values of 95.40 and 93.61 % with the most similar phylogenetic type strains of Rhizobium sophorae CCBAU 03386T and Rhizobium laguerreae FB TT, respectively. The digital DNA–DNA hybridization relatedness values between WYCCWR 11279T and the closest related type strains were less than 70 %. Therefore, a novel rhizobial species is proposed, Rhizobium changzhiense sp. nov., and strain WYCCWR 11279T (=HAMBI 3709T=LMG 31534T) is designated as the type strain for the novel species.

Spatial organization of rural territories

2021 ◽  
pp. 751-756
Author(s):  
Sevostyanov A.V. Sevostyanov A.V. ◽  
V.A. Sevostyanov ◽  
A.P. Spiridonova
Keyword(s):  
Scale Development ◽  
Rural Population ◽  
Large Scale ◽  
Spatial Organization ◽  
Low Density ◽  
Rural Settlements ◽  
Residential Areas ◽  
Complex Development

This article covers the issues raised by the objectives of the "The Program for complex development of rural territories" and its subprogram "Providing rural population with affordable and comfortable housing". The authors substantiate the concept "rural agglomeration" and make the suggestions on how to choose rural settlements and land plots suitable for large-scale development of low-density residential areas.

scholarly journals Establishing Land Suitability Criteria for Cashew (Anacardium occidentaleL.) in Indonesia

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Widiatmaka ◽  
Atang Sutandi ◽  
Anas Iswandi ◽  
Usman Daras ◽  
Muhammad Hikmat ◽  
...  
Keyword(s):  
Scale Development ◽  
Site Selection ◽  
Large Scale ◽  
Land Suitability ◽  
Productivity Index ◽  
Internal Boundary ◽  
Yield Response ◽  
Boundary Line ◽  
The Relationship ◽  
Boundary Lines

Commodity development requires site selection which should be established prior to large scale development. The land suitability criteria for cashew are not presently available. The relationship between the biophysical aspects, especially land and soil with commodity productivity, is also not known in depth. The objective of this study is to establish the criteria of land suitability for cashew in Indonesia, based on its production and land characteristics. Cashew plantations in 5 provinces were sampled. The data of production per tree per year were obtained from farmers, while the soil was sampled and analyzed in the laboratory. Age-adjusted cashew production was used as the yield response and plotted against land characteristics. Boundary lines resulting from the scatter of points were described; these lines produced the limits of land suitability criteria. The criteria were established using a projection of the intersection between the boundary line and yield interval. The criteria were also built in accordance with the productivity index of FAO for the internal boundary inside the S (suitable) class and by calculating the break-event point production for the boundary between S (suitable) and N (nonsuitable) order. The main result of this research is land suitability criteria for cashew.

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