scholarly journals Assessment of a novel pretreatment techniques for enhancing the enzymatic saccharification of sugarcane baggase: Structural and chemical analysis

2020 ◽  
Author(s):  
Soni Tiwari ◽  
Janardan Yadav ◽  
Rajeeva Gaur ◽  
Jay Shanker Yadav
Keyword(s):  
Sugarcane Bagasse ◽  
Enzymatic Reaction ◽  
Enzymatic Saccharification ◽  
Sem Analysis ◽  
Tween 20 ◽  
Acid Pretreatment ◽  
Xylanase Production ◽  
Structure Changes ◽  
Naoh Solution ◽  
Pretreated Sugarcane Bagasse

Abstract Background Enhancement of cellulase and xylanase production and improvement of more proficient lignocellulose-degrading enzymes are essential in order to decrease the price of enzymes required in the biomass-to-bioethanol production. Results The effectiveness of different concentration of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of cellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% cellulose, 25.63% hemicelluloses, 30.36% lignin, 1.44% ash and 2.90% other extractives. Afterthat, The sugarcane bagasse was pretreated with two different concentrations (5% and 10%) of H 2 SO 4 and NaOH at 121°C for 60 min. Among them, the best result was obtained when sugarcane bagasse was pretreated with 10% NaOH solution followed by 10% H 2 SO 4 , 5% NaOH and 5% H 2 SO 4 solution. The highest cellulose saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75mg/g, 301.25 mg/g and 276.6 mg/g from 10% H 2 SO 4 , 5% NaOH and 5% H 2 SO 4 , respectively, which were 55.1, 32.0, 27.1 and 20.6 times higher than the control. Moreover, the FTIR, XRD and SEM analysis showed significant molecule and surface structure changes of the sugarcane bagasse after different pretreatments. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] was used to hydrolyze the pretreated sugarcane bagasse and the optimal condition was determined to be 30 h of enzymatic reaction with 3:1 ration of enzymes under the temperature of 55°C, pH 5.5, substrate concentration of 3% and Tween-20 0.5%. Conclusion Enzyme supernatants produced by the mixed culture of Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] on various pretreated sugarcane baggase have good cellulase and xylanase activities, leading to celluloses and Hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient.

scholarly journals Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

2020 ◽  
Author(s):  
Soni Tiwari ◽  
Janardan Yadav ◽  
Rajeeva Gaur ◽  
Jay Shanker Yadav
Keyword(s):  
Sugarcane Bagasse ◽  
Enzymatic Reaction ◽  
Enzymatic Saccharification ◽  
Sem Analysis ◽  
Tween 20 ◽  
Acid Pretreatment ◽  
Xylanase Production ◽  
Structure Changes ◽  
Naoh Solution ◽  
Pretreated Sugarcane Bagasse

Abstract Background: Enhancement of cellulase and xylanase production and improvement of more proficient lignocellulose-degrading enzymes are essential in order to decrease the price of enzymes required in the biomass-to-bioethanol production. Results: The effectiveness of different concentration of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of cellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% cellulose, 25.63% hemicelluloses, 30.36% lignin, 1.44% ash and 2.90% other extractives. Afterthat, The sugarcane bagasse was pretreated with two different concentrations (5% and 10%) of H2SO4 and NaOH at 121°C for 60 min. Among them, the best result was obtained when sugarcane bagasse was pretreated with 10% NaOH solution followed by 10% H2SO4, 5% NaOH and 5% H2SO4 solution. The highest cellulose saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75mg/g, 301.25 mg/g and 276.6 mg/g from 10% H2SO4, 5% NaOH and 5% H2SO4, respectively, which were 55.1, 32.0, 27.1 and 20.6 times higher than the control. Moreover, the FTIR, XRD and SEM analysis showed significant molecule and surface structure changes of the sugarcane bagasse after different pretreatments. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] was used to hydrolyze the pretreated sugarcane bagasse and the optimal condition was determined to be 30 h of enzymatic reaction with 3:1 ration of enzymes under the temperature of 55°C, pH 5.5, substrate concentration of 3% and Tween-20 0.5%. Conclusion: Enzyme supernatants produced by the mixed culture of Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] on various pretreated sugarcane baggase have good cellulase and xylanase activities, leading to celluloses and Hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient.

scholarly journals Multistep Structural and Chemical Evaluation of Sugarcane Baggase, Pretreated With Alkali for Enhancing the Enzymatic Saccharification by Cellulase and Xylanase of the Pseudomonas sp. CVB-10 (MK443365) and Bacillus paramycoides T4 (MN370035) Mix-Culture System

2022 ◽  
Vol 9 ◽  
Author(s):  
Soni Tiwari ◽  
Janardan Yadav ◽  
Rajeeva Gaur ◽  
Ranjan Singh ◽  
Tuhina Verma ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Enzymatic Reaction ◽  
Enzymatic Saccharification ◽  
Alkali Pretreatment ◽  
Acid Pretreatment ◽  
Chemical Evaluation ◽  
Naoh Pretreatment ◽  
Structure Changes ◽  
Pretreated Bagasse ◽  
Sem Analyses

The enzymatic saccharification of sugarcane bagasse was significantly increased by alkali pretreatment under mild conditions. The effectiveness of different concentrations of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of lignocellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% celluloses, 25.63% hemicelluloses, and 30.36% lignin. After that, sugarcane bagasse was pretreated with 5 and 10% of H2SO4 and NaOH at 121°C for 60 min. FTIR, XRD, and SEM analyses also showed significant molecular and surface structure changes of the sugarcane bagasse with 10% NaOH. Maximum saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75, 301.25, and 276.6 mg/g from 10% H2SO4, 5% NaOH, and 5% H2SO4, respectively, which were 55.1, 32.0, 27.1, and 20.6 times higher than the that of the control. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 (MK443365) and Bacillus paramycoides T4 (MN370035) were used to hydrolyze the pretreated bagasse, and the optimal condition was determined to be 30 h of the enzymatic reaction with the 3:1 ratio of enzymes under the temperature of 55°C, pH 5.0, and substrate concentration of 3%, leading to celluloses and hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient.

scholarly journals Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Vaibhav V. Goud ◽  
Venkata V. Dasu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Crystallinity Index ◽  
Sem Analysis ◽  
Reducing Sugar ◽  
Dilute Acid Pretreatment ◽  
Crystalline Cellulose ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

Enzymatic saccharification of acid-alkali pretreated sugarcane bagasse using commercial enzyme preparations

2012 ◽  
Vol 88 (7) ◽  
pp. 1266-1272 ◽  
Author(s):  
Ellen C. Giese ◽  
Maurício Pierozzi ◽  
Kelly J. Dussán ◽  
Anuj K. Chandel ◽  
Silvio S. da Silva
Keyword(s):  
Sugarcane Bagasse ◽  
Enzymatic Saccharification ◽  
Enzyme Preparations ◽  
Commercial Enzyme ◽  
Pretreated Sugarcane Bagasse

scholarly journals Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vihang S. Thite ◽  
Anuradha S. Nerurkar
Keyword(s):  
Cell Wall ◽  
Sugarcane Bagasse ◽  
Structural Changes ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Gravimetric Analysis ◽  
Chemical Pretreatment ◽  
Cell Wall Degrading Enzymes ◽  
First Time ◽  
Soluble Components

Abstract After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.

scholarly journals Improvement of Enzymatic Saccharification of Cellulose-Containing Raw Materials Using Aspergillus niger

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1360
Author(s):  
Ekaterina Budenkova ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Olga Babich ◽  
Vyacheslav Dolganyuk ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Filter Paper ◽  
Raw Materials ◽  
Enzymatic Saccharification ◽  
Wood Chip ◽  
Cellulase Activity ◽  
Wood Chips ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (control), by ultraviolet (UV) irradiations for 45, 60, or 120 min (UV45, UV60, or UV120), or by UV irradiation for 120 min followed by a chemical treatment with NaN3 + ItBr for 30 min or 80 min (UV120 + CH30 or UV120 + CH80). A mixture of all the A. niger strains (MIX) was also tested. A citrate buffer, at 50 mM, wasthe most suitable for enzymatic hydrolysis. As the UV exposure time increased to 2 h, the cellulase activity of the surviving culturewas increased (r = 0.706; p < 0.05). The enzymatic activities of the obtained strains, towards miscanthus cellulose, wood chips, and filter paper, were inferior to those obtained with commercial enzymes (8.6 versus 9.1 IU), in some cases. Under stationary hydrolysis at 37 °C, pH = 4.7, the enzymatic activity of A. niger UV120 + CH30 was 24.9 IU. The enzymatic hydrolysis of secondary raw materials, using treated A. niger strains, was themost effective at 37 °C. Similarly, the most effective treatment of miscanthus cellulose and wood chips occurred at 50 °C. The maximum conversion of cellulose to glucose was observed using miscanthus cellulose (with alkaline pretreatment), and the minimum conversion was observed when using wood chips. The greatest value of cellulase activity was evidenced in the starch-containing raw materials, indicating that A. niger can ferment not only through cellulase activity, but also via an amylolytic one.

scholarly journals Hot Compressed Water Pretreatment and Surfactant Effect on Enzymatic Hydrolysis Using Agave Bagasse

Energies ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4746
Author(s):  
Marcela Sofia Pino ◽  
Michele Michelin ◽  
Rosa M. Rodríguez-Jasso ◽  
Alfredo Oliva-Taravilla ◽  
José A. Teixeira ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Production Efficiency ◽  
Alcoholic Beverage ◽  
Enzymatic Saccharification ◽  
Raw Material ◽  
Tween 20 ◽  
Ionic Surfactants ◽  
Peg 400 ◽  
Water Pretreatment ◽  
Hot Compressed Water

Agave bagasse is a residual biomass in the production of the alcoholic beverage tequila, and therefore, it is a promising raw material in the development of biorefineries using hot compressed water pretreatment (hydrothermal processing). Surfactants application has been frequently reported as an alternative to enhance monomeric sugars production efficiency and as a possibility to reduce the enzyme loading required. Nevertheless, the surfactant’s action mechanisms in the enzymatic hydrolysis is still not elucidated. In this work, hot compressed water pretreatment was applied on agave bagasse for biomass fractionation at 194 °C in isothermal regime for 30 min, and the effect of non-ionic surfactants (Tween 20, Tween 80, Span 80, and Polyethylene glycol (PEG 400)) was studied as a potential enhancer of enzymatic saccharification of hydrothermally pretreated solids of agave bagasse (AGB). It was found that non-ionic surfactants show an improvement in the conversion yield of cellulose to glucose (100%) and production of glucose (79.76 g/L) at 15 FPU/g glucan, the highest enhancement obtained being 7% regarding the control (no surfactant addition), using PEG 400 as an additive. The use of surfactants allows improving the production of fermentable sugars for the development of second-generation biorefineries.

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