scholarly journals Maternal Karyogene and Cytoplasmic Genotype Affect the Induction Efficiency of Doubled Haploid Inducer in Brassica napus

2021 ◽  
Author(s):  
Wei Zhang ◽  
YongTing Ma ◽  
ZhenDong Zhu ◽  
LiangJun Huang ◽  
Asif Ali ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Low Frequency ◽  
Genotypic Differences ◽  
Induction Effect ◽  
Haploid Induction ◽  
Ssr Analysis ◽  
Induction Process ◽  
Pure Lines ◽  
Chip Analysis ◽  
F1 Generation

Abstract Background Production of pure lines by double haploid induction provides a new way to achieve homozygosity earlier in Brassica napus. Previously, it is not known, whether the induction has obvious maternal genotypic differences. Results In this study, different karyogene and cytoplasmic genotype of Brassica napus was pollinated with the inducers Y3380 and Y3560. Using the identification of the ploidy, fertility and SSR analysis of the induced F1 generation, it was found that the ploidy and phenotype of the induced F1 plants were consistent with maternal parent. The SNP chip analysis revealed that induction efficiency was affected by the karyogene when the maternal cytoplasmic genotype was the same. However, this efficiency was affected by the cytoplasmic genotype when the karyogenes were same, and the offspring of the ogura cytoplasm easier to high-frequency inducer gene hybridization or low-frequency infiltration. Conclusion The induction effect is influenced by the interaction between maternal karyogene and cytoplasmic genotype, and the results from the partial hybridization of progeny chromosomes indicate that the induction process may be attributed to the selective elimination of paternal chromosome. This study provides a basis for exploring the mechanism of DH inducer in Brassica napus, and provides new insights for utilization of inducers inbreeding.

scholarly journals Maternal karyogene and cytoplasmic genotype affect the induction efficiency of doubled haploid inducer in Brassica napus

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Wei Zhang ◽  
Yongting Ma ◽  
Zhendong Zhu ◽  
Liangjun Huang ◽  
Asif Ali ◽  
...  
Keyword(s):  
Low Frequency ◽  
Genotypic Differences ◽  
Induction Effect ◽  
Genome Doubling ◽  
Induction Process ◽  
Selective Elimination ◽  
Paternal Parent ◽  
Ploidy Identification ◽  
Pure Lines ◽  
Chip Analysis

Abstract Background Artificial synthesis of octoploid rapeseed double haploid (DH) induction lines Y3380 and Y3560 was made possible by interspecific hybridization and genome doubling techniques. Production of pure lines by DH induction provides a new way to achieve homozygosity earlier in B.napus. Previously, the mechanism of induction, and whether the induction has obvious maternal genotypic differences or not, are not known so far. Results In this study, different karyogene and cytoplasmic genotype of B.napus were pollinated with the previously reported DH inducers e.g. Y3380 and Y3560. Our study presents a fine comparison of different cytoplasmic genotypes hybridization to unravel the mechanism of DH induction. Ploidy identification, fertility and SSR marker analysis of induced F1 generation, revealed that ploidy and phenotype of the induced F1 plants were consistent with that type of maternal, rather than paternal parent. The SNP chip analysis revealed that induction efficiency of DH inducers were affected by the karyogene when the maternal cytoplasmic genotypes were the same. However, DH induction efficiency was also affected by cytoplasmic genotype when the karyogenes were same, and the offspring of the ogura cytoplasm showed high frequency inducer gene hybridization or low-frequency infiltration. Conclusion The induction effect is influenced by the interaction between maternal karyogene and cytoplasmic genotype, and the results from the partial hybridization of progeny chromosomes indicate that the induction process may be attributed to the selective elimination of paternal chromosome. This study provides a basis for exploring the mechanism of DH inducer in B.napus, and provides new insights for utilization of inducers in molecular breeding.

scholarly journals Genetic Relationships Among Brassica napus Crops Based on SSR Markers

HortScience ◽  
2006 ◽  
Vol 41 (5) ◽  
pp. 1195-1199 ◽  
Author(s):  
Pilar Soengas ◽  
Pablo Velasco ◽  
Guillermo Padilla ◽  
Amando Ordás ◽  
Maria Elena Cartea
Keyword(s):  
Brassica Napus ◽  
Genetic Basis ◽  
Genetic Relationships ◽  
Similarity Coefficient ◽  
Human Consumption ◽  
Genotypic Differences ◽  
Northwestern Spain ◽  
Sources Of Variation ◽  
Relationship Of ◽  
The Relationship

Brassica napus includes economically important crops such as oilseed rape, rutabaga, and leaf rape. Other vegetable forms of Brassica napus, namely nabicol and couve-nabiça, are grown in northwestern Spain and north of Portugal, respectively, and their leaves are used for human consumption and fodder. The relationship of nabicol with other Brassica napus leafy crops was studied before, but its origin remained unclear. The aims of this work were to study the genetic relationships among nabicol landraces and other B. napus crops based on microsatellites and to relate the genotypic differences with the use of the crop. The relationship among 35 Brassica napus populations representing different crops was studied based on 16 microsatellite markers. An analysis of molecular variance was performed partitioning the total variance into three components. The source of variation resulting from groups was defined considering the main use of the crop and accounted for a smaller percentage of variation than other sources of variation, proving that this division is not real. Populations clustered into seven different clusters using a similarity coefficient of 0.82. No clear association was evident between clusters and the main use of populations, suggesting genetic differences among populations could reflect differences in their origin/breeding or domestication. Spanish nabicol could have originated from a sample of couve-nabiças, and couve-nabiças could be used to improve nabicol landraces, because they have a narrow genetic basis that limits their potential for breeding.

Crosswell Magnetic Sensing of Superparamagnetic Nanoparticles for Subsurface Applications

SPE Journal ◽  
2015 ◽  
Vol 20 (05) ◽  
pp. 1067-1082 ◽  
Author(s):  
Amir Reza Rahmani ◽  
Steve Bryant ◽  
Chun Huh ◽  
Alex Athey ◽  
Mohsen Ahmadian ◽  
...  
Keyword(s):  
Magnetic Dipole ◽  
Low Frequency ◽  
Superparamagnetic Nanoparticles ◽  
Dipole Source ◽  
Hydrodynamic Dispersion ◽  
Magnetic Response ◽  
Induction Effect ◽  
Low Frequencies ◽  
Early Stages ◽  
Magnetic Contrast

Summary Stable dispersions of superparamagnetic nanoparticles that are already in use in biomedicine as image-enhancing agents also have potential use in subsurface applications. Surface-coated nanoparticles are capable of flowing through micron-sized pores across long distances in a reservoir, with modest retention in rock. These particles change the magnetic permeability of the flooded region, and thus one can use them to enhance images of the flood. In this paper, we model the propagation of a “ferrofluid” slug in a reservoir and its response to a crosswell magnetic tomography system. This approach to monitoring fluid movement within a reservoir is built on established electromagnetic (EM) conductivity-monitoring technology. In this work, however, we investigate the contrast between injected and resident fluids when they have different magnetic permeabilities. Specifically, we highlight the magnetic response at low frequency to the magnetic excitations generated by a vertical magnetic dipole source positioned at the injection well. At these frequencies, the induction effect is small, the casing effect is manageable, the crosswell response originates purely from the magnetic contrast in the formation, and changes in fluid conductivities are irrelevant. The sensitivity of the measurements to the magnetic slug is highest when the slug is closest to the source or receivers and lower when the slug is midway in the interwell region. At low frequencies, the magnetic response of the ferrofluid slug is largely independent of frequency. As expected for the conductive slug, the sensitivity of the inductive measurements is negligible at low frequencies whereas significant levels of detectability result at higher frequencies. We demonstrate sensitivity to the vertical boundaries of the slug by shifting the vertical position of the excitation source relative to the magnetic slug. The slug geometry plays a key role in determining the magnetic response. With a fixed volume of ferrofluid, there is an optimum slug geometry that results in the maximum magnetic response. Hydrodynamic dispersion of the slug has negligible effect on the magnetic response during early stages of the waterflood. As the slug travels farther into the formation, however, dispersion reduces the concentration of nanoparticles, and the spatial contributions of the magnetic measurements are more diffuse. We illustrate how these low-frequency excitation behaviors are consistent with the quasistatic magnetic dipole physics. The fact that the progress of the magnetic slug can be detected at very early stages of the flood, that the traveling slug's vertical boundaries can be identified at low frequencies, and that the magnetic nanoparticles can be sensed well before the actual arrival of the slug at the observer well provide significant value of the use of the magnetic-contrast agents in crosswell EM tomography.

scholarly journals Analysis of diseases affecting winter and spring forms of Brassica napus L. and Brassica juncea L. in the central zone of the Krasnodar region

2021 ◽  
Vol 937 (3) ◽  
pp. 032114
Author(s):  
O A Serdyuk ◽  
V S Trubina ◽  
L A Gorlova
Keyword(s):  
Brassica Napus ◽  
Brassica Juncea ◽  
High Frequency ◽  
Bacterial Blight ◽  
Low Frequency ◽  
Central Zone ◽  
Occurrence Frequency ◽  
Brassica Napus L ◽  
White Rust ◽  
Krasnodar Region

Abstract The purpose of the research was a comparative evaluation of the disease affection of spring and winter forms of Brassica napus and Brassica juncea on the central zone of the Krasnodar region. Phytosanitary monitoring of diseases was carried out from the seedling stage. In 2011-2020, there were identified the diseases affecting winter and spring crops Brassica napus and Brassica juncea to the same extent: the occurrence frequency of downy mildew and powdery mildew was high, of Alternaria blight – from medium to high, of phytoplasma and bacterial blight – low. Differences are established for Sclerotinia disease, Phoma rot, Fusarium blight, white rust, gray rot. The occurrence frequency of Sclerotinia disease on winter form of Brassica napus and Brassica juncea varied in research years from low to medium, of Phoma rot – from medium to high; on spring forms, these diseases were noted in certain years with the low frequency. Fusarium blight affected only sowings of spring forms of Brassica napus and Brassica juncea with medium and high frequency, except for 2018-2019, when it was low. White rust affected only spring from of Brassica napus, gray rot – only winter forms of Brassica napus and Brassica juncea in certain years with the low frequency.

Genotypic differences in antioxidant response to phosphorus deficiency in Brassica napus

2015 ◽  
Vol 391 (1-2) ◽  
pp. 19-32 ◽  
Author(s):  
Shuisen Chen ◽  
Hua Zhao ◽  
Guangda Ding ◽  
Fangsen Xu
Keyword(s):  
Brassica Napus ◽  
Phosphorus Deficiency ◽  
Antioxidant Response ◽  
Genotypic Differences

scholarly journals A Transformer-Less Multiconverter Having Output Voltage and Frequency Regulation Characteristics, Employed with Simple Switching Algorithms

2021 ◽  
Vol 11 (7) ◽  
pp. 3075
Author(s):  
Naveed Ashraf ◽  
Ghulam Abbas ◽  
Irfan Khan ◽  
Ali Raza ◽  
Nasim Ullah
Keyword(s):  
Output Voltage ◽  
Low Frequency ◽  
Frequency Regulation ◽  
Main Concern ◽  
Power Circuit ◽  
Control Techniques ◽  
Induction Process ◽  
Input Transformer ◽  
Circuit Components ◽  
Ac Converters

The attractive features of the direct AC-AC converters increase their use in many applications such as voltage control for a heavy-duty load that has a high time constant, AC machine drives, and heating systems based on the induction process. These converters process power in single-stage having simple circuit topologies with reduced switching devices and circuit components. These characteristics lead to the efficient power conversion process. The use of a low-frequency input transformer with multiple output tapping for the regulation of output voltage and frequency is one of the major sources of cost, size, and conversion losses. The complication in the switching algorithms is also the main concern in these converters. The preceding deficiencies lower their potency to be used in daily life. The costly controllers or processors are to be employed to realize the complex control techniques or algorithms. That increases the overall cost and circuit complication. This paper introduces the simple control techniques employed to a novel transformer less multi converter to have the various ac outputs for voltage and frequency regulation. The validation of power circuit and control schemes is tested through the simulation and practical results obtained in Simulink and practical setup respectively.

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