Dislocation of the penis: a rare case of genital trauma

Traumatic dislocation of the penis is one of the rarest types of genital trauma. This type of injury is accompanied by a violation of the integrity of the skin and the penile ligamentous apparatus, with the dislocation of the penis into the scrotum under the skin of the thigh, or the area of the pubic joint. The low occurrence frequency of such injuries and the small number of observations described in the literature entails the absence of generally accepted treatment tactics for this category of patients. The article describes a clinical case of successfully treated traumatic dislocation of the penis with penile transposition into the pubic joint area and the formation of subcutaneous urinary leakage after late treatment of the patient for medical care.

The frequency method in studying habitat preferences of common forest birds

This paper presents the results of a study on the habitat preferences of selected species of the bird community in the Morgi Forest, the Kolbuszowa Forest Division (SE Poland), with the use of the point-stand bird census method. The aim of the study was to test the effectiveness of the method in determining the frequency of colonisation of stands with different habitat parameters by the most abundant bird species. In 270 tree stands of a forest complex with diverse habitats, a bird census was carried out with four counts per each stand. Next, a list of the tree stands and the bird species recorded in the stands was compiled. The stands were divided into categories according to the forest habitat type, dominant species and age class. In the next step, the occurrence frequency of the most abundant bird species was calculated for each stand category. Among the analysed species, the majority showed a positive correlation between the frequency of occurrence and habitat fertility. The influence of the dominant stand species on the occurrence frequency of bird species was largely driven by habitat fertility. The lowest average frequency of the identified avian species was found in stands dominated by pine Pinus sylvestris, birch Betula sp. and black alder Alnus glutinosa. There was generally a positive relationship between age class and the bird community parameters. It is concluded that the point-stand method of bird census provides informative results for research on the habitat selectivity of bird populations.

Mitochondrial Transfer in Saccharomyces cerevisiae

<p>This thesis investigated mitochondrial transfer in Saccharomyces cerevisiae, between respiratory compromised B18p⁰ recipient and respiratory competent donor cells. The respiratory compromised strain had three red fluorescent proteins tagged to the membrane, nucleus and cytoplasm (triple RFP-B18p⁰) and is referred to as the B18p⁰ strain. B18p⁰ cells did not contain mitochondrial DNA, causing it to be respiratory compromised and required a fermentable carbon source, such as glucose/dextrose, for proliferation. The respiratory competent strain used had a green fluorescent protein tagged to the Tom70 mitochondrial protein (Tom70-GFP) and is referred to as the Tom70 strain. The Tom70 cells contained the nuclear encoded URA3 cassette, allowing for negative selectivity of this strain using 5-FOA. S. cerevisiae strains were co-cultured together in media containing only non-fermentable carbon sources (YPGE), plated on YPGE plates containing 5-FOA and colonies grown were distinguished post-co-culture based on their distinct phenotypic and genotypic characteristics. Fluorescent analysis of co-culture colonies revealed the presence of 5-FOA resistant Tom70 cells and some red B18p⁰ cells that had acquired the ability to grow on non-fermentable carbon sources. Genotypic analysis revealed that the majority of these red colonies had acquired mtDNA as well as the nuclear encoded, Tom70 specific URA3 cassette. Several permutations of co-cultures were performed, using different ratios of recipient and donor cells and single-gene deletion donor cells. Purified mitochondria from Tom70 cells were tried to be transferred into B18p⁰ cells using centrifugation forces to induce a higher occurrence frequency of mitochondrial transfer. Metabolic support experiments were conducted to investigate if the Tom70 strain could provide metabolic support to the B18p⁰ strain without mitochondrial transfer. Results indicate that no permutation induced potential mitochondrial transfer at a higher rate than others. However, results indicate that mitochondrial transfer did occur at low frequencies, potentially through the fusion of respiratory competent and respiratory compromised cells. Forced transfer did not increase the occurrence frequency of B18p⁰ cells to take up mitochondria and Tom70 cells did not provide metabolic support to B18p⁰ cells.</p>

Mitochondrial Transfer in Saccharomyces cerevisiae

<p>This thesis investigated mitochondrial transfer in Saccharomyces cerevisiae, between respiratory compromised B18p⁰ recipient and respiratory competent donor cells. The respiratory compromised strain had three red fluorescent proteins tagged to the membrane, nucleus and cytoplasm (triple RFP-B18p⁰) and is referred to as the B18p⁰ strain. B18p⁰ cells did not contain mitochondrial DNA, causing it to be respiratory compromised and required a fermentable carbon source, such as glucose/dextrose, for proliferation. The respiratory competent strain used had a green fluorescent protein tagged to the Tom70 mitochondrial protein (Tom70-GFP) and is referred to as the Tom70 strain. The Tom70 cells contained the nuclear encoded URA3 cassette, allowing for negative selectivity of this strain using 5-FOA. S. cerevisiae strains were co-cultured together in media containing only non-fermentable carbon sources (YPGE), plated on YPGE plates containing 5-FOA and colonies grown were distinguished post-co-culture based on their distinct phenotypic and genotypic characteristics. Fluorescent analysis of co-culture colonies revealed the presence of 5-FOA resistant Tom70 cells and some red B18p⁰ cells that had acquired the ability to grow on non-fermentable carbon sources. Genotypic analysis revealed that the majority of these red colonies had acquired mtDNA as well as the nuclear encoded, Tom70 specific URA3 cassette. Several permutations of co-cultures were performed, using different ratios of recipient and donor cells and single-gene deletion donor cells. Purified mitochondria from Tom70 cells were tried to be transferred into B18p⁰ cells using centrifugation forces to induce a higher occurrence frequency of mitochondrial transfer. Metabolic support experiments were conducted to investigate if the Tom70 strain could provide metabolic support to the B18p⁰ strain without mitochondrial transfer. Results indicate that no permutation induced potential mitochondrial transfer at a higher rate than others. However, results indicate that mitochondrial transfer did occur at low frequencies, potentially through the fusion of respiratory competent and respiratory compromised cells. Forced transfer did not increase the occurrence frequency of B18p⁰ cells to take up mitochondria and Tom70 cells did not provide metabolic support to B18p⁰ cells.</p>

Analysis of diseases affecting winter and spring forms of Brassica napus L. and Brassica juncea L. in the central zone of the Krasnodar region

The purpose of the research was a comparative evaluation of the disease affection of spring and winter forms of Brassica napus and Brassica juncea on the central zone of the Krasnodar region. Phytosanitary monitoring of diseases was carried out from the seedling stage. In 2011-2020, there were identified the diseases affecting winter and spring crops Brassica napus and Brassica juncea to the same extent: the occurrence frequency of downy mildew and powdery mildew was high, of Alternaria blight – from medium to high, of phytoplasma and bacterial blight – low. Differences are established for Sclerotinia disease, Phoma rot, Fusarium blight, white rust, gray rot. The occurrence frequency of Sclerotinia disease on winter form of Brassica napus and Brassica juncea varied in research years from low to medium, of Phoma rot – from medium to high; on spring forms, these diseases were noted in certain years with the low frequency. Fusarium blight affected only sowings of spring forms of Brassica napus and Brassica juncea with medium and high frequency, except for 2018-2019, when it was low. White rust affected only spring from of Brassica napus, gray rot – only winter forms of Brassica napus and Brassica juncea in certain years with the low frequency.

NEW RECORDS AND RARE TAXA FOR THE FRESHWATER EPILITHIC DIATOMS OF COTE DIVOIRE

The aim of the present study was to contribute to knowledge of the freshwater epilithic diatoms flora of Cote dIvoire. Diatoms were sampled in February and July 2012 on glass slides previously immersed during a period of 30 days at ten stations.The species composition of new and rare taxa was compiled, accompanied by illustrations. Forty two taxa distributed among 16 genera, 8 families and 5 orders were recorded in the temporal survey.Based on species occurrence frequency, 32 rare, 2 occasional, and 8 common species were registered. According to geographic distribution, taxa recorded were mostly cosmopolitan (47.72%) and tropical (43.18%), while 9.09% were endemic.Taking into account their affinity towards pH, 3 classes of diatoms : acidophilic taxa (35.47%), indifferent taxa (59.52%) and alkaliphilic taxa (4.76%)were found in the Me River.

MJO propagation over the Indian Ocean and Western Pacific in CMIP5 Models: Roles of Background States

This study explored the impacts of background states on the propagation of the Madden-Julian Oscillation (MJO) in 24 CMIP5 models using a precipitation-based MJO tracking method. The ability of the model to reproduce the MJO propagation is reflected in the occurrence frequency of individual MJO events. Moisture budget analysis suggests that the occurrence frequencies of MJO events that propagate across the Indian Ocean (IO-MJO) and western Pacific (WP-MJO) in the models are mainly related to the low-level meridional moisture advection ahead of the MJO convection center. This advection is tightly associated with the background distribution of low-level moisture. Drier biases in background low-level moisture over the entire tropical regions account for underestimated MJO occurrence frequency in the bottom-tier simulations. This study highlights the importance of reproducing the year-to-year background states for the simulations of MJO propagation in the models by further decomposing the background states into the climatology and anomaly components. The background meridional moisture gradient account for the IO-MJO occurrence frequency is closely related to its climatology component, however, the anomaly component regulated by the El Niño–Southern Oscillation (ENSO) is also important for the WP-MJO occurrence frequency. The year-to-year variations of background zonal and meridional gradients associated with ENSO account for the IO-MJO occurrence frequency tend to be offset with each other. As a result, the ENSO shows no significant impact on the IO-MJO occurrence frequency. However, the MJO events tend to more likely propagate across the western Pacific during El Niño years.

A climatology of trade-wind cumulus cold pools and their link to mesoscale cloud organization

We present a climatology of trade cumulus cold pools and their associated changes in surface weather, vertical velocity and cloudiness based on more than 10 years of in situ and remote sensing data from the Barbados Cloud Observatory. Cold pools are identified by abrupt drops in surface temperature, and the mesoscale organization pattern is classified by a neural network algorithm based on Geostationary Operational Environmental Satellite 16 (GOES-16) Advanced Baseline Imager (ABI) infrared images. We find cold pools to be ubiquitous in the winter trades – they are present about 7.8 % of the time and occur on 73 % of days. Cold pools with stronger temperature drops (ΔT) are associated with deeper clouds, stronger precipitation, downdrafts and humidity drops, stronger wind gusts and updrafts at the onset of their front, and larger cloud cover compared to weaker cold pools, which agrees well with the conceptual picture of cold pools. The rain duration in the front is the best predictor of ΔT and explains 36 % of its variability. The mesoscale organization pattern has a strong influence on the occurrence frequency of cold pools. Fish has the largest cold-pool fraction (12.8 % of the time), followed by Flowers and Gravel (9.9 % and 7.2 %) and lastly Sugar (1.6 %). Fish cold pools are also significantly stronger and longer-lasting compared to the other patterns, while Gravel cold pools are associated with significantly stronger updrafts and deeper cloud-top height maxima. The diel cycle of the occurrence frequency of Gravel, Flowers, and Fish can explain a large fraction of the diel cycle in the cold-pool occurrence as well as the pronounced extension of the diel cycle of shallow convection into the early afternoon by cold pools. Overall, we find cold-pool periods to be ∼ 90 % cloudier relative to the average winter trades. Also, the wake of cold pools is characterized by above-average cloudiness, suggesting that mesoscale arcs enclosing broad clear-sky areas are an exception. A better understanding of how cold pools interact with and shape their environment could therefore be valuable to understand cloud cover variability in the trades.