Is it necessary for suspected children with coronavirus disease-2019(COVID-19) to perform CT scan?

Abstract Objectives: the aim is to evaluate justification for suspected children withcoronavirus disease-2019 (COVID-19) to perform CT scanning. Methods: Eighteen suspected children (7 male and 11 female) accepted both CT scanning (except one) and then pharyngeal swab assays from 26th January to 9th March 2020 in the retrospective study. They were confirmed COVID-19 by twice positive test and divided into two groups: asymptomatic subgroup and symptomatic subgroup. Fisher`s exact test was used in statistical analysis. Results: The CT positive ratio was not significant in the two subgroups (0 (0%) vs 3 (23.1%), p=0.541), the same as that in different sex and ages (female vs male: 2 (20.0%) vs 1 (14.3%), p=1.000; <=3 years vs >3 years: 2 (28.6%) vs 1 (10.0%), p=0.537). The demonstrations of the two positive CT were multiple patchy infiltration or ill-fined ground-glass opacity in subpleural regions. Conclusions: It was unnecessary for suspected children with COVID-19 to perform CT scanning as a “routine” ahead of viral nucleic acid assays.

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CT features of COVID-19 patients with two consecutive negative RT-PCR tests after treatment

10.21203/rs.3.rs-33163/v1 ◽

2020 ◽

Author(s):

Zhao Fu ◽

Ningning Tang ◽

Yanqing Chen ◽

Longbai Ma ◽

Youyong Wei ◽

...

Keyword(s):

Nucleic Acid ◽

Ground Glass Opacity ◽

Ct Scanning ◽

Critical Group ◽

Rt Pcr ◽

Pulmonary Lesions ◽

Pleural Thickening ◽

Linear Pattern ◽

Ct Features ◽

Nucleic Acid Tests

Abstract Purpose: The objective of this study is to expound the CT features of COVID-19 patients whose nucleic acid tests converted to negative after treatment.Materials and Methods: We retrospectively reviewed 46 COVID-19 patients with two consecutive negative RT-PCR tests after treatment. The cases were divided into moderate group and severe/critical group according to disease severity. Clinical and CT scanning data were collected. CT signs of pulmonary lesions and the score of long involvement were expounded.Results: 39 moderate cases and 7 severe/critical cases were included. All moderate patients showed peripheral lesions while severe/critical cases exhibited both central and peripheral lesions with all lobes involvement. Ground glass opacity (GGO) and mixed GGO were observed. Aberrant pulmonary interstitium manifested as reticular and thin linear pattern. Thickened blood vessels and pleural thickening were found. Pulmonary fibrosis, annular thickening of the bronchial wall, bronchiectasis, air bronchogram and small amount of bilateral pleural effusion were observed in severe/critical patients. The severe/critical group showed higher CT score of involvement.Conclusions: Pulmonary lesions persisted even after twice consecutive nucleic acid tests converted to negative. We strongly recommended regular follow-up of CT scans after nucleic acid tests conversion. Evaluation of complete remission should base on chest CT.

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The diagnosis of hepatitis C viral infection

Orvosi Hetilap ◽

10.1556/oh.2014.29972 ◽

2014 ◽

Vol 155 (26) ◽

pp. 1019-1023

Author(s):

Judit Gervain

Keyword(s):

Hepatitis C ◽

Nucleic Acid ◽

Viral Infection ◽

Structural Changes ◽

Viral Infections ◽

Transient Elastography ◽

Viral Nucleic Acid ◽

Length Of Treatment ◽

Subtype B

The successful therapy of hepatitis C viral infection requires that the illness is diagnosed before the development of structural changes of the liver. Testing is stepwise consisting of screening, diagnosis, and anti-viral therapy follow-up. For these steps there are different biochemical, serological, histological and molecular biological methods available. For screening, alanine aminotransferase and anti-HCV tests are used. The diagnosis of infection is confirmed using real-time polymerase chain reaction of the viral nucleic acid. Before initiation of the therapy liver biopsy is recommended to determine the level of structural changes in the liver. Alternatively, transient elastography or blood biomarkers may be also used for this purpose. Differential diagnosis should exclude the co-existence of other viral infections and chronic hepatitis due to other origin, with special attention to the presence of autoantibodies. The outcome of the antiviral therapy and the length of treatment are mainly determined by the viral genotype. In Hungary, most patients are infected with genotype 1, subtype b. The polymorphism type that occurs in the single nucleotide located next to the interleukin 28B region in chromosome 19 and the viral polymorphism type Q80K for infection with HCV 1a serve as predictive therapeutic markers. The follow-up of therapy is based on the quantitative determination of viral nucleic acid according to national and international protocols and should use the same method and laboratory throughout the treatment of an individual patient. Orv. Hetil., 2014, 155(26), 1019–1023.

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Non-Viral Nucleic Acid Delivery: Key Challenges and Future Directions

Current Drug Delivery ◽

10.2174/156720111795256174 ◽

2011 ◽

Vol 8 (3) ◽

pp. 235-244 ◽

Cited By ~ 129

Author(s):

Mahmoud Elsabahy ◽

Adil Nazarali ◽

Marianna Foldvari

Keyword(s):

Nucleic Acid ◽

Nucleic Acid Delivery ◽

Viral Nucleic Acid ◽

Future Directions

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Peri-Implant Behavior of Tissue Level Dental Implants with a Convergent Neck

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18105232 ◽

2021 ◽

Vol 18 (10) ◽

pp. 5232

Author(s):

Rubén Agustín-Panadero ◽

Irene Bermúdez-Mulet ◽

Lucía Fernández-Estevan ◽

María Fernanda Solá-Ruíz ◽

Rocío Marco-Pitarch ◽

...

Keyword(s):

Retrospective Study ◽

Statistical Analysis ◽

Bone Loss ◽

Tissue Level ◽

Functional Load ◽

Implant Placement ◽

Group I ◽

Bone Level ◽

Single Crowns ◽

Functional Loading

Introduction: The aim of this retrospective study was to analyze the radiographic peri-implant bone loss of bone level implants and tissue level implants with a convergent neck in screw-retained single crowns and in screw-retained fixed partial prostheses, after two years of functional loading. Materials and methods: The sample was divided into two groups according to their type: Group I: supracrestal implants with convergent transmucosal neck; Group II: crestal implants. In each group we distinguish two subgroups according to the type of prosthetic restoration: single crowns and a three-piece fixed partial prosthesis on two implants. To quantify bone loss, parallelized periapical radiographs were analyzed at the time of implant placement and after two years of functional load. Results: A total of 120 implants were placed in 53 patients. After statistical analysis it was observed that for each type of implant bone loss was 0.97 ± 0.91 mm for bone level and 0.31 ± 0.48 mm for tissue level. No significant differences were found regarding the type of prosthesis and the location (maxilla or mandible) of the implants. Conclusions: Tissue level implants with a convergent transepithelial neck exhibit less peri-implant bone loss than bone level implants regardless of the type of prosthesis.

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In pursuit of sensitivity: Lessons learned from viral nucleic acid detection and quantification on the raindance ddPCR platform

Methods ◽

10.1016/j.ymeth.2021.04.008 ◽

2021 ◽

Author(s):

Samuel Long

Keyword(s):

Nucleic Acid ◽

Lessons Learned ◽

Nucleic Acid Detection ◽

Viral Nucleic Acid ◽

Detection And Quantification

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Sonographic Signs of Adenomyosis in Women with Endometriosis Are Associated with Infertility

Journal of Clinical Medicine ◽

10.3390/jcm10112355 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2355

Author(s):

Dean Decter ◽

Nissim Arbib ◽

Hila Markovitz ◽

Daniel S. Seidman ◽

Vered H. Eisenberg

Keyword(s):

Laparoscopic Surgery ◽

Retrospective Study ◽

Statistical Analysis ◽

Conservative Management ◽

Transvaginal Ultrasound ◽

Control Group ◽

Study Group ◽

Control Groups ◽

Increased Risk ◽

And Control

We compared the prevalence of ultrasound signs of adenomyosis in women with endometriosis who underwent surgery to those who were managed conservatively. This was a retrospective study of women evaluated at a tertiary endometriosis referral center who underwent 2D/3D transvaginal ultrasound. Adenomyosis diagnosis was based on the presence of at least three sonographic signs. The study group subsequently underwent laparoscopic surgery while the control group continued conservative management. Statistical analysis compared the two groups for demographics, symptoms, clinical data, and sonographic findings. The study and control groups included 244 and 158 women, respectively. The presence of any, 3+, or 5+ sonographic signs of adenomyosis was significantly more prevalent in the study group (OR = 1.93–2.7, p < 0.004, 95% CI; 1.24–4.09). After controlling for age, for all findings but linear striations, the OR for having a specific feature was higher in the study group. Women in the study group with ≥ 5 sonographic signs of adenomyosis had more than twice the risk of experiencing infertility (OR = 2.31, p = 0.012, 95% CI; 1.20–4.45). Sonographic signs of adenomyosis are more prevalent in women with symptomatic endometriosis who underwent surgery compared with those who continued conservative management. Women with 5+ findings have a significantly increased risk of infertility. Adenomyosis on ultrasound should be considered in the management decisions regarding these patients.

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Influence of the COVID-19 Pandemic on Dental Emergency Admissions in an Urgent Dental Care Service in North Italy

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18041812 ◽

2021 ◽

Vol 18 (4) ◽

pp. 1812 ◽

Cited By ~ 1

Author(s):

Maria Grazia Cagetti ◽

Araxi Balian ◽

Nicole Camoni ◽

Guglielmo Campus

Keyword(s):

Retrospective Study ◽

Dental Care ◽

Tooth Extraction ◽

Care Service ◽

Emergency Admissions ◽

Exact Test ◽

Remarkable Increase ◽

Time Period ◽

Dental Emergency ◽

Second Wave

A retrospective study was performed to verify if the number of admissions for urgent dental care in the Urgent Dental Care Service of San Paolo Hospital in Milan (Italy) was directly related to the different phases of the COVID-19 pandemic. Different periods were analyzed: 25 March–5 April 2019 (pre-COVID); 23 March–3 April 2020 (lockdown); 8 June–19 June 2020 (reopening); and November 9–November 20 (second wave). Raw data regarding admissions, diagnoses, and treatments were extracted. Descriptive and bivariate analyses were performed. The survey included 901 admissions, 285 in pre-COVID, 93 during lockdown, 353 in reopening, and 170 in the second wave. In each time period, statistically significant differences were found in the prevalence of each kind of diagnoses (χ2(3) = 20.33 p = 0.01 for endodontic emergencies, χ2(3) = 29.05 p < 0.01 for cellulitis/phlegmon, χ2(3) = 28.55 p < 0.01 for periodontal emergencies, Fisher’s Exact Test p < 0.01 for trauma, and χ2(3) = 59.94 p < 0.01 for all other kinds of diagnosis). A remarkable increase in consultations (+186.36%) and other treatments (+90.63%) occurred during reopening. Tooth extraction was the most frequently delivered treatment, but suffered the largest reduction during lockdown (−79.82%). The COVID-19 pandemic has highly affected dental activity in north Italy, underling the weaknesses of a private dental system in a pandemic scenario.

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Viral nucleic acid sequences in transformed cells

Virology ◽

10.1016/0042-6822(76)90174-4 ◽

1976 ◽

Vol 72 (2) ◽

pp. 456-470 ◽

Cited By ~ 68

Author(s):

S.J. Flint ◽

Joe Sambrook ◽

J.F. Williams ◽

Phillip A. Sharp

Keyword(s):

Nucleic Acid ◽

Transformed Cells ◽

Viral Nucleic Acid ◽

Nucleic Acid Sequences

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Silver nanocluster-lightened hybridization chain reaction

RSC Advances ◽

10.1039/c6ra09337j ◽

2016 ◽

Vol 6 (62) ◽

pp. 57502-57506 ◽

Cited By ~ 3

Author(s):

Lin Liu ◽

Qing Li ◽

Li-Juan Tang ◽

Ru-Qin Yu ◽

Jian-Hui Jiang

Keyword(s):

Nucleic Acid ◽

Silver Nanoclusters ◽

Hybridization Chain Reaction ◽

Label Free ◽

Chain Reaction ◽

Silver Nanocluster ◽

Turn On ◽

Nucleic Acid Assays

A hybridization chain reaction (HCR) lightened by DNA-stabilized silver nanoclusters (AgNCs) as a label-free and turn on fluorescence platform for nucleic acid assays.

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