scholarly journals Molecular Detection of Some Toxigenic Cyanobacteria in Tigris River in Baghdad–Iraq

2021 ◽  
Author(s):  
Noor Redha Gummaa ◽  
Ahmed Sahi Dwaish ◽  
Israa Hussein Hamzah
Keyword(s):  
Drinking Water ◽  
Molecular Detection ◽  
Gene Fragment ◽  
Nostoc Commune ◽  
Pcr Assay ◽  
The Public ◽  
Tigris River ◽  
The World ◽  
Westiellopsis Prolifica ◽  
Algal Toxin

Abstract Cyanobacteria and their emissions are becoming more widely reported around the world, posing a serious threat to both the environment and human health. Several orders of cyanobacteria have been identified to make cyanotoxin, the most common algal toxin. The aim of this research was to develop a method for detecting cylindrosprmopsin and saxitoxin biosynthesis genes in rivers .In November, December 2019 and January2020. Cyanobacteria were isolated from Tigris River freshwater and detected using a compound microscope as well as traditional PCR .All cyanobacteria isolates contained phycocyanin gene fragment. Five isolates of cyanobacteria in these study was successfully amplified a phycocyanin gene (Microcystis flosaquae, Microcystis sp, anabaena circinalis ,nostoc commune and westiellopsis prolifica) and all isolates successfully amplified aoaC gene to detecting the cylidrospemopsin and the saxitoxin. Our findings show that a PCR assay can be used to detect cylidrospemopsin and saxitoxin-producing cyanobacteria in river water, which is useful for stations that prepare drinking water for the public.

scholarly journals Abilities of the mCP Agar Method and CRENAME Alpha Toxin-Specific Real-Time PCR Assay To Detect Clostridium perfringens Spores in Drinking Water

2013 ◽  
Vol 79 (24) ◽  
pp. 7654-7661 ◽  
Author(s):  
Andrée F. Maheux ◽  
Ève Bérubé ◽  
Dominique K. Boudreau ◽  
Romain Villéger ◽  
Philippe Cantin ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Sample ◽  
Real Time ◽  
Clostridium Perfringens ◽  
Real Time Pcr ◽  
Potable Water ◽  
Alpha Toxin ◽  
Pcr Assay ◽  
Content Type ◽  
The Public

ABSTRACTWe first determined the analytical specificity and ubiquity (i.e., the ability to detect all or most strains) of aClostridium perfringens-specific real-time PCR (rtPCR) assay based on thecpagene (cpartPCR) by using a bacterial strain panel composed ofC. perfringensand non-C. perfringens Clostridiumstrains. All non-C. perfringens Clostridiumstrains tested negative, whereas allC. perfringensstrains tested positive with thecpartPCR, for an analytical specificity and ubiquity of 100%. ThecpartPCR assay was then used to confirm the identity of 116 putativeC. perfringensisolates recovered after filtration of water samples and culture on mCP agar. Colonies presenting discordant results between the phenotype on mCP agar andcpartPCR were identified by sequencing the 16S rRNA andcpagenes. Four mCP−/rtPCR+colonies were identified asC. perfringens, whereas 3 mCP+/rtPCR−colonies were identified as non-C. perfringens. ThecpartPCR was negative with all 51 non-C. perfringensstrains and positive with 64 of 65C. perfringensstrains. Finally, we compared mCP agar and a CRENAME (concentration andrecovery of microbial particles,extraction ofnucleicacids, andmolecularenrichment) procedure pluscpartPCR (CRENAME +cpartPCR) for their abilities to detectC. perfringensspores in drinking water. CRENAME +cpartPCR detected as few as oneC. perfringensCFU per 100 ml of drinking water sample in less than 5 h, whereas mCP agar took at least 25 h to deliver results. CRENAME +cpartPCR also allows the simultaneous and sensitive detection ofEscherichia coliandC. perfringensfrom the same potable water sample. In itself, it could be used to assess the public health risk posed by drinking water potentially contaminated with pathogens more resistant to disinfection.

scholarly journals DRINKING WATER POLLUTION IN INDIA - A CHALLENGE FOR HUMAN HEALTH

2015 ◽  
Vol 3 (9SE) ◽  
pp. 1-5
Author(s):  
Anjana Jain
Keyword(s):  
Drinking Water ◽  
Drinking Water Treatment ◽  
World Health ◽  
Polluted Water ◽  
Negative Effects ◽  
The Public ◽  
Main Challenge ◽  
The World ◽  
The Government ◽  
Health Organization

According to the latest report of the World Health Organization, 7 lakh 83 thousand people die due to polluted water every year in India. The main challenge of the Government of India is to provide clean water to the public. Therefore, in the study presented, an attempt has been made to know why there is pollution of drinking water, what types of diseases are caused by this polluted water, d. An attempt has been made to know its negative effects and pure drinking water treatment. The data and facts have been compiled on a secondary basis. विष्व स्वास्थ संगठन की ताजा रिपोर्ट के अनुसार भारत में प्रतिवर्ष 7 लाख 83 हजार लोग प्रदूषित जल की वजह से मरते है। जनता को साफ पानी पहुचाना भारत सरकार की मुख्य चुनौति है। अतः प्रस्तुत अध्ययन में यह जानने का प्रयास किया गया है कि पेयजल प्रदुषण क्यों होता है घ् इस प्रदूषित जल से किस प्रकार की बीमारियाँ उत्पन्न होती है घ् इसके नकारात्मक प्रभाव एवं शुध्द पेयजल उपचार को जानने का प्रयास किया गया है। आंकड़ो व तथ्यों का संकलन द्वितीयक आधार पर किया गया है।

scholarly journals An international review of the challenges associated with securing buy-in for water safety plans within providers of drinking water supplies

2010 ◽  
Vol 8 (2) ◽  
pp. 387-398 ◽  
Author(s):  
Corinna Summerill ◽  
Jen Smith ◽  
James Webster ◽  
Simon Pollard
Keyword(s):  
Drinking Water ◽  
World Health Organisation ◽  
World Health ◽  
International Review ◽  
Water Safety ◽  
Financial Efficiency ◽  
The Public ◽  
Quality Guidelines ◽  
The World ◽  
Water Safety Plans

Since publication of the 3rd Edition of the World Health Organisation (WHO) Drinking Water Quality guidelines, global adoption of water safety plans (WSPs) has been gathering momentum. Most guidance lists managerial commitment and ‘buy-in’ as critical to the success of WSP implementation; yet the detail on how to generate it is lacking. This commentary discusses aspects of managerial commitment to WSPs. We argue that the public health motivator should be clearer and a paramount objective and not lost among other, albeit legitimate, drivers such as political or regulatory pressures and financial efficiency.

Emerging Contaminants in Drinking Water: Should the Public Be Concerned?

2009 ◽  
Vol 4 (4) ◽  
Author(s):  
M. Giddings ◽  
J. Fawell
Keyword(s):  
Drinking Water ◽  
Media Coverage ◽  
Endocrine Disruptor ◽  
Emerging Contaminants ◽  
Public Concern ◽  
Water Supplies ◽  
The Public ◽  
The World ◽  
Public Concerns

Significant media coverage regarding new chemicals being found in water supplies has given rise to public concerns about the safety of their drinking water. Emerging contaminants in drinking water supplies, which include pharmaceuticals, personal care products and endocrine disruptor compounds, have been regularly detected at trace levels in drinking water sources in many countries. Regulators around the world are grappling with questions from the public relating to possible health outcomes from long-term exposure to these contaminants in their drinking water. The presentation will discuss the challenges in addressing public concern about these contaminants and how communicating scientific uncertainties requires a balanced and clear series of messages.

scholarly journals Molecular and Immunological Methods to Confirm Toxiginicity (Microcystin Production) of Westiellopsis Prolifica Isolated from Tigris River – Iraq

2019 ◽  
Vol 16 (4(Suppl.)) ◽  
pp. 0978
Author(s):  
Abed Et al.
Keyword(s):  
Laboratory Experiments ◽  
Immunological Methods ◽  
Laboratory Methods ◽  
Tigris River ◽  
Cyanobacterial Species ◽  
The World ◽  
Mcye Gene ◽  
Microcystin Production ◽  
Westiellopsis Prolifica ◽  
Significant Expression

Several toxigenic cyanobacteria produce the cyanotoxin (microcystin). Being a health and environmental hazard, screening of water sources for the presence of microcystin is increasingly becoming a recommended environmental procedure in many countries of the world. This study was conducted to assess the ability of freshwater cyanobacterial species Westiellopsis prolifica to produce microcystins in Iraqi freshwaters via using molecular and immunological tools. The toxigenicity of W. prolifica was compared via laboratory experiments with other dominant bloom-forming cyanobacteria isolated from the Tigris River: Microcystis aeruginosa, Chroococcus turigidus, Nostoc carneum, and Lyngbya sp. significant expression of mcyE gene and microcystin production was most evident in W. prolifica. Contrary to the prevailing concept that M. aeruginosa is a main microcystin producer in freshwaters around the world, no significant microcystin production was observed with this species throughout the time points studied in our laboratory methods. As for C. turigidus, N. carneum and Lyngbya sp., neither mcyE expression nor microcystin production was significant. Data from mcyE expression by RT-qPCR were generally in agreement with those obtained from microcystin quantification by ELISA. Interestingly, W. prolifica, which showed clear microcystin-producing ability in this study and which was not reported before in the literature to produce microcystin, can be added as a new microcystin producer to the list of toxigenic cyanobacteria.

Environmental Contamination of Arsenic and its Toxicological Impact on Humans

2005 ◽  
Vol 2 (3) ◽  
pp. 146 ◽  
Author(s):  
Jack C. Ng
Keyword(s):  
Developing Countries ◽  
Drinking Water ◽  
Arsenic Speciation ◽  
Inorganic Arsenic ◽  
Chemical Forms ◽  
The Public ◽  
Epidemiologic Evidence ◽  
The World ◽  
Suitable Animal Model ◽  
Real Challenge

Environmental Context. Tens of millions of people in developing countries are being exposed to excessive levels of arsenic in their drinking water, and this contamination is widely regarded as the largest current calamity of chemical poisoning in the world. However, arsenic can exist in many chemical forms, and these vary widely in solubility, toxicity, and in bioavailability. Therefore, it is critical to be able to measure arsenic speciation accurately and reliably in order to understand its toxicity and design effective measures of remedial action. Abstract. Inorganic arsenic compounds are known carcinogens. The human epidemiologic evidence of arsenic-induced skin, lung, and bladder cancers is strong. However, the evidence of arsenic carcinogenicity in animals is very limited. Lack of a suitable animal model until recent years has inhibited studies of the mechanism of arsenic carcinogenesis. The toxicity and bioavailability of arsenic depend on its solubility and chemical forms. Therefore, it is critical to be able to measure arsenic speciation accurately and reliably. However, speciation of arsenic in more complex matrices remains a real challenge. There are tens of millions of people who are being exposed to excessive levels of arsenic in the drinking water alone. The source of contamination is mainly of natural origin and the mass poisoning is occurring worldwide, particularly in developing countries. Chronic arsenicosis resulting in cancer and non-cancer diseases will impact significantly on the public health systems in their respective countries. Effective watershed management and remediation technologies in addition to medical treatment are urgently needed in order to avoid what has been regarded as the largest calamity of chemical poisoning in the world.

scholarly journals Influence of Environmental Factors on Occurrence of Cyanobacteria and Abundance of Saxitoxin-Producing Cyanobacteria in a Subtropical Drinking Water Reservoir in Brazil

Water ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1716
Author(s):  
Munique Moraes ◽  
Raphaella Rodrigues ◽  
Louise Schlüter ◽  
Raju Podduturi ◽  
Niels Jørgensen ◽  
...  
Keyword(s):  
Drinking Water ◽  
Environmental Variables ◽  
Molecular Detection ◽  
Water Reservoir ◽  
Pcr Assay ◽  
Robust Approach ◽  
Drinking Water Reservoir ◽  
Toxic Cyanobacteria ◽  
Tn:Tp Ratio ◽  
Quantitative Pcr Assay

Blooms of cyanobacteria are frequent in Brazilian water reservoirs used for drinking water. The warning for the presence of potential toxin-producing cyanobacteria is typically based on time-consuming microscopy, rather than specific molecular detection of toxic genes in cyanobacteria. In this study, we developed a quantitative PCR assay for the detection of cyanobacteria producing the neurotoxin saxitoxin (STX). The assay targets the sxtA gene in the sxt gene cluster. Potential and dominant STX-producers in the Itupararanga reservoir were the genera Raphidiopsis, Aphanizomenon and Geitlerinema. Numbers of the sxtA gene varied from 6.76 × 103 to 7.33 × 105 cells mL−1 and correlated positively with SXT concentrations in the water. Concentrations of STX and the sxtA gene also correlated positively with TN:TP ratio and pH, but correlated negatively with inorganic nutrients and turbidity, confirming that regulation of the SXT production was impacted by environmental variables. In contrast, the occurrence of another cyanotoxin, microcystin, did not correlate with any environmental variables. The developed qPCR assay was found to be a rapid and robust approach for the specific quantification of potential STX-producing cyanobacteria and should be considered in future investigations on toxic cyanobacteria to provide an early warning of potential toxin episodes.

“All in a Garden Green”: Shakespeare's Staging of Garden Imagery

2019 ◽  
Vol 26 (2) ◽  
pp. 227-252
Author(s):  
Deborah Solomon
Keyword(s):  
Early Modern ◽  
Literary Tradition ◽  
The Public ◽  
The World ◽  
Audience Interaction ◽  
The Many ◽  
Green World

This essay draws attention to the surprising lack of scholarship on the staging of garden scenes in Shakespeare's oeuvre. In particular, it explores how garden scenes promote collaborative acts of audience agency and present new renditions of the familiar early modern contrast between the public and the private. Too often the mention of Shakespeare's gardens calls to mind literal rather than literary interpretations: the work of garden enthusiasts like Henry Ellacombe, Eleanour Sinclair Rohde, and Caroline Spurgeon, who present their copious gatherings of plant and flower references as proof that Shakespeare was a garden lover, or the many “Shakespeare Gardens” around the world, bringing to life such lists of plant references. This essay instead seeks to locate Shakespeare's garden imagery within a literary tradition more complex than these literalizations of Shakespeare's “flowers” would suggest. To stage a garden during the sixteenth and seventeenth centuries signified much more than a personal affinity for the green world; it served as a way of engaging time-honored literary comparisons between poetic forms, methods of audience interaction, and types of media. Through its metaphoric evocation of the commonplace tradition, in which flowers double as textual cuttings to be picked, revised, judged, and displayed, the staged garden offered a way to dramatize the tensions produced by creative practices involving collaborative composition and audience agency.

Vitruvian Character: The Case of the Egyptian Museum

2014 ◽  
Vol 8 (3) ◽  
pp. 105
Author(s):  
Khaled Asfour
Keyword(s):  
Local Community ◽  
British Museum ◽  
Spatial Experience ◽  
The Public ◽  
Cultural Boundaries ◽  
The World

In Vitruvius’ treatise, what makes good architecture is its ability to communicate to the public particular messages that reflects the program of the building with spaces and components arranged in an orderly way. According to Vitruvius these messages when acknowledges by the public the building posses strong character. This research discusses this idea by reflecting on the 1895 competition of the Egyptian Museum project. Marcel Dourgnon, the French architect of the winning scheme, showed profound understanding of character resulting in a building that had positive vibe with the local community.  Today Vitruvius’ idea is still living with us. Norman Foster succeeded in upgrading the British Museum in a way that addressed all cultures of the world through his grand atrium design.  Similarly, Emad Farid and Ramez Azmy revived the presence of the Egyptian Museum in public cognition.  Spatial experience that evokes similar perceptions to all its visitors is a timeless piece that transcends cultural boundaries.

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