Dynamic changes in the rhizosphere bacterial community in monoculture and intercropping maize and soybean during various crop growth stages

Abstract Although rhizosphere microorganisms have been studied for a long time, rhizosphere microbial communities based on monoculture and intercropping soybean and maize have rarely been studied. To define the effect of crop monoculture and intercropping on soil physicochemical properties and rhizosphere bacterial communities, field experiments were conducted using maize and soybean cultivars at five different crop growth stages, including monoculture maize, monoculture soybean and maize-soybean intercropping. The rhizosphere bacterial communities were analyzed by using the 16S rRNA Illumina sequencing. The pH and soil organic matter (SOM) were the key factors affecting crop rhizosphere soil bacterial communities. The intercropping soybean-maize increased the available phosphorus (AP) content at five different crop growth stages. And the available potassium (AK) content in the intercropping soybean soil samples was higher than corresponding monoculture soil samples. The content of available cadmium (ACd) in monoculture soybean rhizosphere soil samples decreased and then increased, but the intercropping soybean soil samples indicated an opposite trend. Proteobacteria, Chloroﬂexi, Acidobacteria, Actinobacteria and Firmicutes were the dominant phyla in the soybean and maize rhizosphere soil samples. Crops of the same plant species showed little difference in the bacterial community diversity under the two planting modes. The results indicated the intercropping planting pattern altered the absorption of ACd in the maize and soybean soil since the S2 stage and showed a different change in different crop growth stages. And the maize-soybean intercropping system also changed the bacterial community and soil physicochemical properties.

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Dynamic changes in the rhizosphere bacterial community in monoculture and intercropped corn and soybean during various crop growth stages

10.21203/rs.3.rs-49276/v2 ◽

2020 ◽

Author(s):

Han Li ◽

Luyun Luo ◽

Bin Tang ◽

Huanle Guo ◽

Zhongyang Cao ◽

...

Keyword(s):

Bacterial Community ◽

Microbial Communities ◽

Rhizosphere Soil ◽

Crop Growth ◽

Soil Samples ◽

Community Diversity ◽

Growth Stages ◽

Cadmium Content ◽

Crop Growth Stages ◽

Rhizosphere Bacterial Community

Abstract Although rhizosphere microorganisms have been studied for a long time, rhizosphere microbial communities based on monoculture and intercropped soybean and corn have rarely been studied. To define the effect of crop monoculture and intercropping on soil physicochemical properties and rhizosphere microbial communities, field experiments were conducted using corn and soybean cultivars at five different crop growth stages. The pH and soil organic matter (SOM) were the key factors affecting crop rhizosphere soil microorganisms. The content of available phosphorus (AP) in the intercropped soybean/corn soil samples was higher than that in the monoculture soil samples at five different growth stages. The content of available cadmium (ACd) in monoculture soybean rhizosphere soil samples decreased and then increased, but the intercropped soybean soil samples indicated an opposite trend. Proteobacteria, Chloroﬂexi, Acidobacteria, Actinobacteria and Firmicutes were the dominant phyla in the soybean and corn rhizosphere soil samples. Crops of the same plant species showed little difference in the bacterial community diversity under the two planting modes. The results showed that the cadmium content in cadmium-polluted soil could be reduced by the soybean/corn intercropping system. The crop rhizosphere bacterial community is not only regulated by the crop itself but also influenced by the planting pattern.

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Dynamic Changes in the Rhizosphere Bacterial Community in Monoculture and Intercropped Corn and Soybean During Various Crop Growth Stages

10.21203/rs.3.rs-49276/v1 ◽

2020 ◽

Author(s):

Han Li ◽

Luyun Luo ◽

Bin Tang ◽

Huanle Guo ◽

Zhongyang Cao ◽

...

Keyword(s):

Bacterial Community ◽

Microbial Communities ◽

Rhizosphere Soil ◽

Crop Growth ◽

Soil Samples ◽

Community Diversity ◽

Growth Stages ◽

Cadmium Content ◽

Crop Growth Stages ◽

Rhizosphere Bacterial Community

Abstract Although rhizosphere microorganisms have been studied for a long time, rhizosphere microbial communities based on monoculture and intercropped soybean and corn have rarely been studied. To define the effect of crop monoculture and intercropping on soil physicochemical properties and rhizosphere microbial communities, field experiments were conducted using corn and soybean cultivars at five different crop growth stages. The pH and soil organic matter (SOM) were the key factors affecting crop rhizosphere soil microorganisms. The content of available phosphorus(AP) in the intercropped soybean/corn soil samples was higher than that in the monoculture soil samples at five different growth stages.The content of available cadmium(ACd) in monoculture soybean rhizosphere soil samples decreased and then increased, but the intercropped soybean soil samples indicated an opposite trend.Proteobacteria, Chloroﬂexi, Acidobacteria, Actinobacteria, and Firmicutes were the dominant phyla in the soybean and cornrhizosphere soil samples. Crops of the same plant species showed little difference in the bacterial community diversity under the two planting modes. The results showed that the cadmium content in cadmium-polluted soil could be reduced by the soybean/corn intercropping system. The crop rhizosphere bacterial community is not only regulated by the crop itself but also influenced by the planting pattern.

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Effects of Sphaeropsis Blight on Rhizosphere Soil Bacterial Community Structure and Soil Physicochemical Properties of Pinus sylvestris var. mongolica in Zhanggutai, China

Forests ◽

10.3390/f10110954 ◽

2019 ◽

Vol 10 (11) ◽

pp. 954

Author(s):

Saiyaremu Halifu ◽

Xun Deng ◽

Xiaoshuang Song ◽

Yuning An ◽

Ruiqing Song

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Pinus Sylvestris ◽

Physicochemical Properties ◽

Bacterial Community Structure ◽

Rhizosphere Soil ◽

Liaoning Province ◽

Soil Physicochemical Properties ◽

Α Diversity ◽

Significant Difference

Pinus sylvestris var. mongolica is an important tree species for ecological construction and environmental restoration owing to its rapid growth rate and excellent stress resistance. Pinus sylvestris var. mongolica sphaeropsis blight is a widespread disease caused by Sphaeropsis sapinea. This study was focused on non-infected (CK) and infected (SS) Pinus sylvestris var. mongolica plants in Zhanggutai area, Liaoning Province, China. Illumina high-throughput sequencing based on the templates of sequencing-by-synthesis working with reversible terminators is a widely used approach. In the present study, systematic differences in relationships among rhizosphere soil physicochemical properties, bacterial community structure, diverse bacterial genera, and alpha diversity indices between the two categories were evaluated. The current findings are as follows: (1) Shannon’s index of SS soil was significantly higher than CK, and it was significantly lower in May than July and September (p < 0.05). (2) Non-metric multidimensional scaling (NMDS) showed a difference in bacterial community structure during May (spring), July (summer), and September. (3) At the phylum level, no significant difference was found in the bacterial genera between CK and SS soil for three seasons; however, at the genus level, there were about 19 different bacterial genera. The correlation studies between 19 different bacterial genera and environmental factors and α-diversity indicated that bacterial genera of non-infected and infected Pinus sylvestris var. mongolica were distributed differently. The bacterial genera with CK were positively correlated with soil physicochemical properties, while a negative correlation was found for SS. In conclusion, the differences in nutrient and microbial community structure in the rhizosphere soil of Pinus sylvestris var. mongolica are the main causes of shoot blight disease.

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Influences of land reclamation on soil bacterial communities of abandoned salt pans in the Yellow River Delta

10.22541/au.163251189.95628732/v1 ◽

2021 ◽

Author(s):

Yihao Zhu ◽

Xiliang Song ◽

Xiaoli Liu ◽

Weifeng Chen ◽

Xuchang Niu ◽

...

Keyword(s):

Bacterial Community ◽

Physicochemical Properties ◽

Soil Nutrients ◽

Bacterial Communities ◽

Land Reclamation ◽

Bacterial Community Composition ◽

Soil Physicochemical Properties ◽

Salt Pans ◽

Α Diversity ◽

Relative Abundances

Reclamation has been widely accepted to restore abandoned lands. Most studies focused on the improvement of land reclamation in soil nutrients and microbial activities. However, the effects of reclamation time on bacterial communities of abandoned salt pans are still unclear. The object of this study is to: i) assess the successional change of soil physicochemical properties and bacterial communities in reclaimed abandoned salt pans with different reclamation histories, and ii) figure out the main limit factors on the improvement of soil quality in reclaimed abandoned salt pans. The soils in a farmland (RTBL) and six abandoned salt pans with 1 year (RT1), 2 years (RT2), 3 years (RT3), 4 years (RT4), 8 years (RT8), and 9 years (RT9) of reclamation were sampled to investigate the temporal variation of soil properties, heavy metal content, bacterial community composition, and diversity. Results showed that the soil bulk density (BD), total dissolved salt (SS), median particle size (MMAD) decreased with the increase of reclamation time, while soil nutrient (soil organic matter, total nitrogen, available phosphorus, available potassium) showed an opposite trend. The bacterial α-diversity increased first, then decrease. Land reclamation enhanced the relative abundances of Acidobacteria, Chloroflexi, and Actinobacteria but reduced the relative abundances of Proteobacteria, Gemmatimonadetes, and Bacteroidetes. Compared with RTBL, the soil nutrients and bacterial community structure in RT1, RT2, RT3, and RT4 showed a significant difference.Therefore, reclamation time is a vital driving force for restoring soil physicochemical properties and bacterial communities in abandoned

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Sustainability of Urban Soil Management: Analysis of Soil Physicochemical Properties and Bacterial Community Structure under Different Green Space Types

Sustainability ◽

10.3390/su11051395 ◽

2019 ◽

Vol 11 (5) ◽

pp. 1395 ◽

Cited By ~ 1

Author(s):

Junda Zhang ◽

Suyan Li ◽

Xiangyang Sun ◽

Jing Tong ◽

Zhen Fu ◽

...

Keyword(s):

Organic Matter ◽

Soil Moisture ◽

Soil Organic Matter ◽

Bacterial Community ◽

Physicochemical Properties ◽

Total Nitrogen ◽

Bacterial Communities ◽

Green Space ◽

Soil Physicochemical Properties ◽

Soil Bacterial Communities

Soil bacterial communities play a key role in nutrient cycling and ecosystem functioning. This study aims to reveal how green space type impacts soil quality and the bacterial community, which finally contributes to suggesting strategies for managing sustainable environments in urban areas. For this purpose, urban green space soils in this study are divided into four different types; park green space (PARK), street green space (STREET), attached green space (ATTACH) and residential green space (RESID). Results showed that significant differences were observed for soil physicochemical properties. Soil organic matter, total nitrogen, soil moisture content and available nitrogen in the ATTACH and PARK soils were significantly higher than in the STREET and RESID soils. Across the four green space types, the structure of bacterial communities was dominated by Proteobacteria, Actinobacteria and Chloroflexi at the phylum level. The diversity and richness of bacteria were significantly higher in the PARK and ATTACH soils than in the RESID and STREET soils. Results of principal component analysis (PCoA) showed that soil bacterial communities could be clustered into four different groups according to different green space types. In addition, analysis of similarities (ANOSIM) also implied that soil samples differed significantly from others. Redundancy analysis (RDA) and Spearman correlation analysis both showed that the contents of soil organic matter, total nitrogen, soil moisture and pH had great influence on the structures of bacterial communities. In summary, these results suggest that soil physicochemical properties and bacterial communities can be strongly affected by green space types, and thus, objective assessment of a particular measure can be provided to land managers and policy makers for informed decision-making in urban development and sustainability.

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