scholarly journals Enriched Microbial Communities for Ammonium and Nitrite Removal from Recirculating Aquaculture Systems

2021 ◽  
Author(s):  
Alireza Neissi ◽  
Gholamreza Rafiee ◽  
Shadi Rahimi ◽  
Hamid Farahmand ◽  
Santosh Pandit ◽  
...  
Keyword(s):  
Microbial Communities ◽  
High Performance ◽  
Pathogenic Bacteria ◽  
Environmental Water ◽  
Recirculating Aquaculture ◽  
Expression Of Genes ◽  
Recirculating Aquaculture Systems ◽  
Nitrite Oxidizing Bacteria ◽  
Nitrite Removal ◽  
Aquaculture Water

Abstract Background: The aim of this study was the enrichment of high-performance microbial communities in biofilters for removal of ammonium and nitrite from aquaculture water. Methods: Ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were enriched from different environmental water samples. The microbial communities with higher ammonium and nitrite removal activity were selected and adapted to different temperatures [9 ºC, 15 ºC, room temperature (25 ºC), and 30 ºC]. The expression of genes involved in nitrification including ammonia monooxygenase (AMO) and nitrite oxidoreductase (NXR) were measured in temperature-adapted AOB and NOB microbiomes. The microbial species present in the selected microbiomes were identified via 16s rRNA sequencing. Results: The microbial communities containing Nitrosomonas oligotropha and Nitrobacter winogradskyi showed the highest ammonium and nitrite removal activity at all temperatures used for adaptation. Furthermore, the microbial communities do not contain any pathogenic bacteria. They also exhibited the highest expression of AMO and NXR genes. Using the enriched microbial communities, we achieved a 288% and 181% improvement in ammonium and nitrite removal over the commonly used communities in biofilters at 9 °C, respectively. Conclusions: These results suggest that the selected microbiomes allowed for a significant improvement of water quality in a recirculating aquaculture system (RAS).

The Water Quality Management for Recirculating Aquaculture System by Applying Nano Adsorption Technology

2021 ◽  
Vol 21 (7) ◽  
pp. 3975-3979
Author(s):  
Min-Jin Hwang ◽  
Jeongmin Cha ◽  
Eun-Sik Kim
Keyword(s):  
Water Quality ◽  
Water Quality Management ◽  
Reduction Rate ◽  
Fish Farming ◽  
Analytical Relationship ◽  
Pumice Stone ◽  
Recirculating Aquaculture ◽  
Recirculating Aquaculture Systems ◽  
Aquaculture Water

In a fish farm, the water quality is important to ensure fish growth and farm productivity. However, the study of the quality of water using in aquaculture has been ignored until now. Although there are several methods to treat water, nanomaterials have not yet been applied for indoor fish farming because it may difficult to supply a sufficient amount of water, and the operating parameters have not been developed for recirculating aquaculture systems. Nanotechnology can be applied to treat water, specifically through adsorption and filtration, to produce drinking water from surface water and to treat wastewater by processing a high volume of effluent. The adsorption and filtration of seawater has also progressed to allow for desalination of seawater, and this is recognized as a necessary tool for extended treatment protocols of various types of seawater. This study investigated the treatment of aquaculture water using nano-porous adsorbents (e.g., pumice stone) to control the contaminants in seawater in order to maintain the water quality required for aquaculture. The results are used to derive an analytical relationship between the ionic species in aquaculture water, and this provides empirical parameters for a batch reactor for aquaculture. The quality of the influent and effluent for aquaculture is compared using time-series analyses to evaluate the reduction rate of ionic components and thus suggest the optimum condition for fish farming using bioreactor processes.

scholarly journals Bacterial Relationships in Aquaponics: New Research Directions

2019 ◽  
pp. 145-161 ◽  
Author(s):  
Alyssa Joyce ◽  
Mike Timmons ◽  
Simon Goddek ◽  
Timea Pentz
Keyword(s):  
Microbial Communities ◽  
Plant Production ◽  
Microbial Decomposition ◽  
Recirculating Aquaculture ◽  
Recirculating Aquaculture Systems ◽  
Wide Range ◽  
Technical Specifications ◽  
New Research ◽  
System 1

AbstractThe growth rates and welfare of fish and the quality of plant production in aquaponics system rely on the composition and health of the system’s microbiota. The overall productivity depends on technical specifications for water quality and its movement amongst components of the system, including a wide range of parameters  including factors such as pH and flow rates which ensure that microbial components can act effectively in nitrification and remineralization processes. In this chapter, we explore current research examining the role of microbial communities in three units of an aquaponics system: (1) the recirculating aquaculture system (RAS) for fish production which includes biofiltration systems for denitrification; (2) the hydroponics units for plant production; and (3) biofilters and bioreactors, including sludge digester systems (SDS) involved in microbial decomposition and recovery/remineralization of solid wastes. In the various sub-disciplines related to each of these components, there is existing literature about microbial communities and their importance within each system (e.g. recirculating aquaculture systems (RAS), hydroponics, biofilters and digesters), but there is currently limited work examining interactions between these components in aquaponics system, thus making it an important area for further research.

scholarly journals Understanding structure/function relationships in nitrifying microbial communities after cross-transfer between freshwater and seawater

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Blanca M. Gonzalez-Silva ◽  
Kjell Rune Jonassen ◽  
Ingrid Bakke ◽  
Kjetill Østgaard ◽  
Olav Vadstein
Keyword(s):  
Microbial Communities ◽  
Rrna Gene ◽  
Physiological Plasticity ◽  
Oxidation Rates ◽  
Salinity Changes ◽  
Recirculating Aquaculture ◽  
Seawater Salinity ◽  
Recirculating Aquaculture Systems ◽  
Before And After

AbstractIn this study, nitrification before and after abrupt cross-transfer in salinity was investigated in two moving bed biofilm reactors inoculated with nitrifying cultures that had adaptation to freshwater (FR) and seawater salinities (SR). FR and SR MBRRs were exposed to short and long term cross-transfer in salinity, and the functional capacity of nitrifying microbial communities was quantified by the estimation of ammonia and nitrite oxidation rates. Salinity induced successions were evaluated before and after salinity change by deep sequencing of 16S rRNA gene amplicons and statistical analysis. The bacterial community structure was characterized and Venn diagrams were included. The results indicated that after salinity cross-transfer, the FR was not significantly recovered at seawater salinity whereas SR showed high resistance to stress caused by low-salt. Succession and physiological plasticity were the main mechanisms of the long-term adaption of the nitrifying communities exposed to abrupt salinity changes. Independently of salinity, some nitrifiers presented high physiological plasticity towards salinity and were very successful at both zero and full seawater salinity. SR culture is robust and suitable inoculum for ammonium removal from recirculating aquaculture systems and industrial wastewaters with variable and fast salinity changes. Our findings contradict the current perspective of the significance of salinity on the structure of nitrifying communities.

scholarly journals A Temporally Dynamic Gut Microbiome in Atlantic Salmon During Freshwater Recirculating Aquaculture System (RAS) Production and Post-seawater Transfer

2021 ◽  
Vol 8 ◽  
Author(s):  
Marlene Lorgen-Ritchie ◽  
Michael Clarkson ◽  
Lynn Chalmers ◽  
John F. Taylor ◽  
Herve Migaud ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Microbial Communities ◽  
16S Rrna Gene Sequencing ◽  
Fish Growth ◽  
Organic Load ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Recirculating Aquaculture ◽  
Recirculating Aquaculture Systems ◽  
Rrna Gene Sequencing

Atlantic salmon aquaculture is undergoing an expansion of land-based recirculating aquaculture systems (RAS), especially for freshwater (FW) stages of production. Juvenile salmon undergo parr-smolt transformation, also known as smoltification and become pre-adapted to tolerate seawater (SW). One aspect requiring study is the development of microbial communities during this time, especially in RAS systems. Here we analyzed temporal changes in microbiome associated with the intestine in Atlantic salmon during smolt production in a commercial RAS production facility and followed the same cohort of fish post-seawater transfer (SWT), using 16S rRNA gene sequencing. Microbial diversity and richness showed an increase over time across FW production, but declined sharply and significantly 1-week post-SWT before re-establishing itself with a completely different community structure after 4 weeks. Core microbial taxa could be assigned to three distinct categories; (1) omnipresent, (2) salinity specific, or (3) transient. By including diet and water samples in the analyses, we classified true core taxa associated with the host, those associated with the diet, and transient cores associated with microbial communities in tank water. The rising trend observed in microbial richness in the water may be a consequence of a temporal increase in organic load while dominance of Vibrionaceae may be attributed to the higher temperatures maintained during RAS production and above average natural water temperatures post-SWT. Functional analysis suggests modulation of metabolic pathways post-SWT, but downstream impacts on fish growth and health in a commercial setting remain to be elucidated. A deeper understanding of the interplay between microbial composition and functionality can play a role in optimizing fish performance in tightly regulated RAS production.

scholarly journals Feed Supplementation with a Commercially Available Probiotic Solution Does Not Alter the Composition of the Microbiome in the Biofilters of Recirculating Aquaculture Systems

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 830
Author(s):  
Simon Menanteau-Ledouble ◽  
Rui A. Gonçalves ◽  
Mansour El-Matbouli
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Flora ◽  
Clarias Gariepinus ◽  
Farming Systems ◽  
Detectable Effect ◽  
African Catfish ◽  
Fish Feed ◽  
Feed Supplementation ◽  
Recirculating Aquaculture ◽  
Recirculating Aquaculture Systems

Recirculating aquaculture relies on the treatment of ammonia compounds from the water by a bacterial flora growing inside biofilters. Another increasingly common practice in aquaculture is the supplementation of feed with live probiotic bacteria to boost the immune system of the farmed animals and hinder the implantation of pathogenic bacteria. In the present study, we investigated the bacterial flora within the biofilters of recirculating farming units in which African catfish (Clarias gariepinus) were being farmed. Our results suggested that these two farming systems could be compatible as feeding of the probiotic feed had no detectable effect on the composition of the microbiome within the biofilters and none of the bacteria from the feed could be detected in the biofilters. These findings suggest that supplementation of the fish feed with probiotic supplements did not interfere with the microbiome residing inside the biofilter and that it is a safe practice in recirculating aquaculture systems.

scholarly journals A High-Performance Optoelectronic Sensor Device for Nitrate Nitrogen in Recirculating Aquaculture Systems

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3382 ◽  
Author(s):  
Cong Wang ◽  
Zhen Li ◽  
Zhongli Pan ◽  
Daoliang Li
Keyword(s):  
Measurement Errors ◽  
High Performance ◽  
Nitrate Nitrogen ◽  
Living Environment ◽  
Environmental Stability ◽  
Correction Function ◽  
Synchronous Detector ◽  
Frequency Noise ◽  
Recirculating Aquaculture ◽  
Recirculating Aquaculture Systems

The determination of nitrate nitrogen (NO3-N) in recirculating aquaculture systems is of great significance for the health assessment of the living environment of aquatic animals. Unfortunately, the commonly used spectrophotometric methods often yield unstable results, especially when the ambient temperature varies greatly in the field measurement. Here, we have developed a novel handheld absorbance measurement sensor based on the thymol-NO3-N chromogenic rearrangement reaction. In terms of hardware, the sensor adopts a dual channel/dual wavelength colorimeter structure that features a modulated light source transmitter and a synchronous detector receiver. The circuit measures the ratio of light absorbed by the sample and reference containers at two LEDs with peak wavelengths at 420 nm and 450 nm. Using the modulated source and synchronous detector rather than a constant (DC) source eliminates measurement errors due to ambient light and low frequency noise and provides higher accuracy. In terms of software, we design a new quantitative analysis algorithm for absorbance by studying colloid absorbing behavior. The application of a buffer operator embedded in the algorithm makes the sensor get the environmental correction function. The results have shown that the sensitivity, repeatability, precision and environmental stability are higher than that by ordinary spectrophotometry. Lastly, we have a brief overview of future work.

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