scholarly journals Fertilization and Development of Conjoined Human Oocyte from a Binovular Follicle Containing Two Individual Oocytes Surrounded by a Zona Pellucida

Author(s):  
Guangzhong Jiao ◽  
Jianhua Wang ◽  
Lili Chen ◽  
Yinghua Shan ◽  
Hua Xing ◽  
...  

Abstract The most reliable definition of conjoined oocytes is the inclusion of two oocytes within a common zona pellucida(ZP) or their fusion in the zonal region. Because the available data are extremely limited, the significance of conjoined oocytes in IVF laboratory setting has been questionable. We analyzed the characteristics of 18 patients with conjoined oocytes in our center. The maturation of conjoined oocytes were asynchronous. Most of the cases were that one oocyte in each pair of conjoined oocytes was in the MII stage and the other was in the GV stage. The maturation rate and fertilization rate of conjoined oocytes were significantly lower than that of normal oocytes. There was no significant difference in the rate of cleavage and D3 High-quality embryo, but the rate of blastocyst formation of conjoined oocytes decreased significantly. The area of larger oocytes in conjoined oocytes was not different from that of normal oocytes, and its companion was significantly smaller than that of normal oocytes. The mean ZP thickness of all oocytes, larger or smaller, was significantly thinner than that of normal oocytes. After analyzing the clinical characteristics of the patients, it was suggested that the higher estrogen level or the more number of maturation follicles on HCG day were more likely to promote the emergence of conjoined oocytes.

2019 ◽  
Vol 19 (1) ◽  
pp. 115-125
Author(s):  
Nguyen Xuan Thanh ◽  
Luc Van Long ◽  
Tran Thi Thu Trang ◽  
Pham Quoc Hung

This study was conducted to evaluate the effect of dietary, salinity on the reproductive efficiency of mud clam broodstock (Austriella corrugata) in the maturing condition. The reproductive efficiency was assessed by survival rate, maturation rate, actual fecundity, spawning rate, fertilization rate, reproductive efficiency rate (100*D-veliger/fertilized eggs), larval quality. The experiment was held in the five dietaries and three salinities (18‰, 23‰ and 28‰). Three replicates were carried out for each treatment. Results showed that the reproductive efficiency of using fresh algae complex in 28‰ salinity was the highest and the larvae are in good quality, followed by that in the 23‰ salinity but having no statistically significant difference ( p > 0.05) while that in the 18‰ salinity experienced the least (p < 0.05). This study on dietary, salinity suggests that fresh algae complex in the salinity of 28 ± 1‰ is suitable for maturing broodstock of mud clam. These findings will contribute to building up hatchery production techniques as well as helping protection of natural stock and developing sustainable mud clam resource.


2021 ◽  
Author(s):  
Wei Liu ◽  
Lin-Xue Qian ◽  
Xue-Jing wei

Abstract Purpose To investigate clinical and ultrasound features for differentiating ovarian clear cell carcinoma (OCCC) from ovarian high-grade serous carcinoma (HGSC). Methods Forty-five patients with OCCC and 72 patients with HGSC were retrospectively studied. Patient clinical characteristics and ultrasound features of tumors were evaluated. The differences, including laterality, menopausal status, Federation of Gynecology and Obstetrics (FIGO) stage between OCCC and HGSC patients were compared by Fisher’s exact test. The ultrasound features of tumors, including laterality, shape, configuration, color score, peritoneal implantation, and ascites, were evaluated and compared between the two groups. Results The average age at diagnosis in the OCCC group was 57.6±11.1 (range, 30–76) years, and 35.56% of patients were premenopausal. However, the average age at diagnosis was 63.4±12.2 (range, 42-81) years and 33.33% of patients were premenopausal in the HGSC group. There was a statistically significant difference in unilaterality (P< 0.001), clear boundaries (P<0.05), round or oval shape (P <0.05), and color score (P<0.05). Compared to HGSC patients, fewer OCCC patients had peritoneal implantation and ascites. There was a statistically significant difference in the mean size of papillary projections in OCCC and HGSC (P<0.05). The mean size of the papillary projections was significantly larger in OCCC than in HGSC. Conclusions OCCC commonly appeared as a large, round or oval mass with a clear boundary; the papillary projections of OCCC are larger and round. In contrast, HGSC was typically a large, irregular tumor with solid masses or mixed cystic-solid masses with small papillary projections.


Zygote ◽  
1995 ◽  
Vol 3 (2) ◽  
pp. 101-109 ◽  
Author(s):  
Jean Philippe Wolf ◽  
Sylvie Bulwa ◽  
Daniel Rodrigues ◽  
Pierre Jouannet

SummaryThe cytometry of 545 oocytes was evaluated during subzonal insemination (SUZI; 85 attempts), on day 0 (egg retrieval and SUZI), day 1 and day 2(embryo transfer). On day 0, the egg and oolemma diameters (mean ± SD) were 164.0 ± 19.6 μm and 114.2±16.8 μ5m respectively.The zona thickness was 17.8± 13.4 μm and correlated with the oolemma diameter(r = 0.24, p < 0.001). The fertilisation rate was significantly lower for the smaller oocytes (less than 108 μm diameter) compared with the larger oocytes (over 108μm) (9.8% vs 21.2% respectively; p < 0.05). These was little variation in oocyte diameter according to nuclear status. However, oocyte diameter increased significantly between day 0 and day 1 (p < 0.001) for both fertilised and unfertilised oocytes. Six different indications for SUZI were investigated in detail: three with non-specific (normal and subnormal sperm with in vitro fertilization failure, oligoasthenospermia) and three with specific sperm defects (flagellar dyskinesia, absence of outer dynein arms, antisperm antibodies). Oocytes from the non-specific defect groups had significantly smaller diameters than the others (p < 0.05). The mean fertilisation rate was related to the mean oolemma diameter for the groups with non-specific sperm defects and the group lacking dynein arms (LODA) (r = 0.91, p < 0.05). Eggs from the groups of patients with LODA and those with antisperm antibodies had thicker zona pellucida than others (p < 0.05). These findings suggest that in addition to nuclear criteria of maturity, the growth of oocytes is an important factor for fertilising ability. Insufficient development of the ooplasm may contribute to fertilisation failure, particularly when sperm with functional defects are used. In contrast, a thick zona pellucida may prevent sperm with specific anomalies such as LODA or antisperm antibodies from penetrating into the perivitelline space.


Zygote ◽  
2021 ◽  
pp. 1-5
Author(s):  
Nafiye Yılmaz ◽  
Şebnem Özyer ◽  
Derya Taş ◽  
Mehmet Caner Özer ◽  
Ayten Türkkanı ◽  
...  

Summary To determine the fertilization and embryonic potential of immature metaphase I (MI) oocytes in patients with low oocyte maturity rate in whom the percentage of mature oocytes obtained was less than 75% of the total retrieved ones. In vivo matured metaphase II (MII) oocytes (MII-ICSI, n = 244), and in vitro matured MI oocytes (MI-MII-ICSI, n = 202) underwent an intracytoplasmic sperm injection (ICSI) procedure. Maturation rate, fertilization rate and early embryonic development were compared in both groups. In total, 683 oocytes were collected from 117 ICSI cycles of 117 patients. Among them, 244 (35.7%) were mature MII and 259 (37.9%) were MI after the denudation process. Of those 259 MI oocytes, 202 (77.9%) progressed to MII oocytes after an incubation period of 18–24 h. The maturation rate was 77.9%. Fertilization rate was found to be significantly higher in the rescued in vitro matured MI oocyte group when compared with the in vivo matured MII oocyte group (41.6% vs 25.8%; P = 0.0006). However, no significant difference was observed in terms of cleavage rates on days 2 and 3 between the groups (P = 0.9126 and P = 0.5031, respectively). There may be unidentified in vivo factors on the oocyte maturation causing low developmental capacity in spite of high fertilization rates in the group of patients with low oocyte maturity rate. Furthermore, studies are needed to determine the appropriate culture characteristics as well as culture period and ICSI timing of these oocytes.


2017 ◽  
Vol 5 (1) ◽  
Author(s):  
M. Khoiruddin

The objectives of this research are (1) to find out the speaking ability of SMA Negeri 2 Rembang students before being taught using series pictures of storytelling, (2) to find out the speaking ability of SMA Negeri 2 Purwodadi students after being taught using series pictures of storytelling, and (3) to find out whether or not there is a significant difference of speaking ability of SMA Negeri 2 Purwodadi students before and after being taught using series pictures of storytelling.This study uses Experimental method. The number of populations in this research is30 students. The method of data collection is spoken test. First the writer gives pre-test to the students and scores. Second the writer gives a treatment for two meetings at this time, the writer implements the series pictures in classroom activities. At last, the writer gives the students a post test. The data analysis reveals that the mean of pre-test score is 61,03 ; it is at fair level. The mean of post-test score is 76,1 ; it is at good level. Then the result of t-test is9,71, and the t-table of 30 students is2,04. The result of t-test is higher than t-table. Based on research analysis, the writer takes conclusions: first the speaking ability of the students before being taught using series pictures of storytelling is fair, second the speaking ability of the students after being taught using series pictures of storytelling is good, and last there is significant improvement of English speaking ability of the students taught by using series pictures of storytelling. The writer also suggests to all of teachers of senior high school. It is better for them to use the media of series pictures of storytelling to improve the students’ speaking ability. It is for the simple reason that a series pictures are a media to improve the speaking ability at storytelling. The writer also suggests to the students that the study is as recommendation to motivate the students in improving the students’ speaking ability. The students are encouraged to give their opinions, ideas and imagination about the presented pictures. Definition of key terms (1) series picture A picture is an illustration of pictures that can be used as a two dimensional visual representation of percent, place, or things. (2) Media is any extension of man which allows him to affect other people who are not in face to face with him. (3) Story telling is In this research storytelling is used to improve the students’ speaking ability. It caused that storytelling can improve the ideas and imagines their story and express it by telling the story. It helps the students to speak and talk active.


2017 ◽  
Vol 15 (1) ◽  
pp. 20-23
Author(s):  
Morteza Qaribi ◽  
Ali Abdolrazaghnejad ◽  
Reza Shahmirzaei ◽  
Abdolghader Pakniyat

Abstract Introduction. Contrast-induced-nephropathy (CIN) is a common complication during angiography that may lead to long-term complications. This study was conducted to investigate the effect of melatonin administration on prevention of CIN in patients who underwent coronary angiography with intra-arterial contrast agents. Method. This is single-blind randomized clinical trial that was performed over 100 patients with indication for coronary angiography. Patients are randomly assigned to two equal groups. All patients in the 12 hours before and 12 hours after the procedure, were received adequate intravenous hydration with normal saline and for the intervention group in addition to hydration, the day before angiography and immediately after angiography 3 mg melatonin was administered. For all patients, serum level of creatinine (Cr), blood urea nitrogen (BUN) and glomerular filtration rate (GFR) before and 48 hours after the procedure were measured. Data were analyzed using SPSS 18 software. Results. Totally 100 participants with the mean age of 64.0±8.2 years were enrolled (63% male). There was no significant difference between intervention and control groups in baseline and demographic characteristics (P> 0.05). Although the mean serum Cr and BUN level increased in both groups, but the mean Cr, BUN and GFR before and after coronary angiography was not statistically significant. Based on the definition of CIN in the current study, 3(6%) patients from intervention group and 2(4%) patients from control group were affected by CIN (P = 0.243). Conclusion. It is likely that, melatonin administration has no significant effect on prevention of CIN following coronary angiography.


2013 ◽  
Vol 25 (1) ◽  
pp. 159
Author(s):  
F. Becker ◽  
W. Kanitz ◽  
G. Nurnberg ◽  
D. Rath

Different factors determine the effectiveness of the use of sires in AI. Most important factors are the number of inseminated spermatozoa, the quality of spermatozoa, and the time of insemination. Especially in superovulated animals, the insemination scheme plays in important role to cover the whole ovulation period. The influence of 3 different dosages of spermatozoa (15 × 106, 5 × 106, and 1 × 106) on fertilization rate was examined in experiment A. In experiment B, one dosage of female and male spermatozoa of 3 different bulls was used for timed AI in 31 heifers. Timed AI in normal-cycling cattle [13 h after gonadotropin-releasing hormone (GnRH) application] with detected corpus luteum (Days 8 to 13 of cycle) was carried out after induction of luteolysis and induction of ovulation [GnRH application 60 h after prostaglandin F2α (PGF2) application]. Embryos and oocytes were flushed from the oviduct of 116 hemicastrated or slaughtered heifers on Day 4 after insemination. The ovulation rate in heifers was 95.4%. Eighty percent of the oocytes or embryos were recovered. The influence of the factors sire, ejaculate, and dosage were tested by GLM analyses of SAS® (SAS Institute Inc., Cary, NC, USA). There was no significant difference in the fertilization rate (93.3, 96.2, and 78.8%) and in the proportion of normally developed embryos (84.6, 80.7, and 75.8%) between groups. Significant differences were found in the mean number of accessory sperms/embryo and in the proportion of embryos with >10 accessory sperms/embryo or without accessory sperms; however, the proportion of intact embryos was similar. Using sexed semen in experiment B, similar results were obtained after flushing of the oviducts on Day 4 after insemination of hemicastrated or slaughtered animals. In total, an ovulation rate of 91.7%, a recovery rate of 70%, and a fertilization rate of 86.8% were obtained. There were no differences between female- and male-sorted spermatozoa and the control group. In experiment C, altogether 13 heifers were treated 8 times with FSH for 4 days starting between Day 8 to 12 of estrous cycle. Prostaglandin F2α was given 48 and 60 h after the first FSH injection. Insemination with sexed semen (n = 5 heifers) and with unsorted semen (n = 8; 15 × 106 and 1 × 106) was done at 55 and 71 h after induction of luteolysis. Flushing of the uterus was performed on Day 7. Using the time-oriented insemination after superovulation of animals, fertilization rates varied between 65 and 85%. There was no difference between groups regarding the number of transferable embryos (5.5, 4.9, and 4.8). The results demonstrate that the application of an approved insemination schedule may accomplish high fertilization rates after insemination with sexed or reduced dosages of spermatozoa in normal-cycling as well as superovulated cattle.


2015 ◽  
Vol 49 (6) ◽  
Author(s):  
S. S. Deka ◽  
D. J. Kalita ◽  
S. Sarma ◽  
D. J. Dutta

Sixty healthy ovaries were collected from local slaughter house. Oocytes from small and medium sized follicles (2-8 mm in diameter) were selected for oocyte collection using aspiration followed by slicing . A and B category ( cumulus oocyte complexes (COCs) with more than 5 layers and 3-5 layers respectively of compact cumulus cells surrounding the zona pellucida) were selected for <italic>in vitro</italic> maturation. A total of 361 oocytes were found to be matured with overall mean maturation rate of 82.59 ± 0.02%. The mean number of grade A, B and C oocytes recovered per ovary was 4.26 ± 0.53, 3.05 ± 0.31 and 1.25 ± 0.19, respectively. The overall recovery of grade A, B and C oocytes were 256, 183 and 74 . Out of 439 oocytes 58.86 ± 0.05%, 33.23 ±0.04 % and 9.90 ± 0.04 % showed +++, ++ and + degrees of cumulus expansion, respectively. It was concluded that aspiration followed by slicing is an ideal method for maximum yield of oocytes and <italic>in vitro</italic> maturation in indigenous cow of Assam.


2013 ◽  
Vol 12 (1) ◽  
pp. 144
Author(s):  
S. Hadi

Several advantages have been suggested for producing sexed sperms including using fewer and genetically superior female animals for replacement.Four hundred active ovaries collected from the slaughter house of Al-shu'alah, the number and type of oocytes, ratios of maturation and fertilization shown that there was a significant difference in the numbers of oocytes (P<0.05) between right and left ovaries. A high recovery rate was obtained of good oocyte (Grade A) 42.35% (432/1020), fair oocyte (Grade B) 37.54% (383/1020) followed by and poor oocyte (Grade C) 17.84% (182/1020). There was a significant difference (P<0.05) between the 3 different grades. grades A and B oocytes, (815/1020) 79.9% of recovered oocytes were cultured. Maturation rate was 86.38% (704/815).Y- Bearing sperms separation applied by using procedure of the modified albumin technique; either one (8%) or two layers (8 and 16%) of BSA (M1, and M2) at 200, 300 or 400 xg, then used for in vitro fertilization.The in vitro fertilization rate observed was 21.8% (132/604) of matured oocytes by choosing universal primers from sequences that are highly conserved in the X and Y chromosomes, sex-specific sequences were successfully amplified in embryonic lysates. Bovine serum albumin sexed sperms result in more percentage of male embryos by using one layer of BSA ( 8%) at 200 × g (M1a) and 300× g (M1b) which were 72.7% and 54.5% respectively, and shows a deviation (p<0.05) from the 50% expected percentage for male and female embryos. While using two layer of BSA (16% and 8% BSA) at the 200 × g (M2a) and at 300× g (M2b) were 81.8% and 63.6% respectively. When we compare the rate of male embryos produced from IVF by sperms isolated by two layers of BSA (M2a, and M2b), moderate results obtained with M2b (63.6%) while the best results were with M2a separation protocol (81.8%).


2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Houdong Zuo

Objective. To investigate the clinical characteristics and CT findings of parotid and submandibular gland tumours. Materials and methods. From May 2017 to April 2020, all patients with clinically proven parotid and submandibular gland enlargement and palpable masses underwent CT examinations. All patients were confirmed by pathology after surgery. The clinical characteristics and CT features were observed and evaluated. The mean density values before and after enhancement were measured and analyzed. The chi-square test, one-way ANOVA, and Student's t-test were used. Results. Ninety-four patients with a total of 94 unilateral tumours in the parotid and submandibular glands were enrolled, including 38 pleomorphic adenomas (PAs), 27 Warthin’s tumours (WTs), and 29 malignant tumours (MTs). The majority of the PAs (28/38) and MTs (23/29) were located in the parotid gland; the others were located in the submandibular gland. All the WTs were in the parotid gland. The most common benign tumours of the parotid gland were PAs (28/38, 73.7%) and WTs (27/27, 100%), and the most common MTs were mucoepidermoid carcinoma, acinic cell carcinoma, and squamous cell carcinoma (4/29, 13.8%). The most common benign and malignant tumours in the submandibular gland were PAs (10/38, 26.3%) and ductal adenocarcinomas (3/4, 75%). The majority of PA patients (28/38) were female, compared with WT (2/27) ( P < 0.001 ) and malignant tumour patients (10/29) ( P < 0.01 ). A significant difference was also found between WTs and MTs in female patients ( P < 0.05 ). The mean age of PA patients was 43.4 ± 12.1 years, which was lower than that of WTs (62.1 ± 11.7) and MTs (58 ± 14.18) ( P < 0.001 , P < 0.001 , and P = 0.244 , respectively). On CT imaging, the mean diameter of the PAs and WTs was significantly smaller than that of the MTs ( P = 0.001 and P < 0.001 ), and no difference was observed between the PAs and WTs ( P = 0.275 ). In the parotid gland, the superficial lobe was more frequently involved than the deep lobe (PAs, 22 : 6; WTs, 17 : 10; and MTs, 15 : 8). The majority of PAs and WTs demonstrated round shapes (25/38, 19/27) and were well defined (30/38, 24/27); by contrast, most MTs were lobulated, irregular shapes (24/29), and ill defined (25/29). On plain CT, the PAs were usually homogeneous, while MTs were frequently heterogeneous, with more necrosis, larger cystic areas, and more haemorrhage or calcification. The mean CT values of PAs, WTs, and MTs were 39.2 ± 3.9 HU, 39.1 ± 3.0 HU, and 37.6 ± 3.1 HU ( P > 0.05 ), respectively. On contrast CT, the WTs were significantly enhanced compared with MTs and PAs, with mean CT values of 53.5 ± 4.0 HU, 84.4 ± 6.0 HU, and 65.2 ± 3.8 HU, respectively (all P < 0.001 ). The mean CT value changes for PAs, WTs, and MTs (∆) were 14.4 ± 3.0 HU, 45.3 ± 4.5 HU, and 27.7 ± 2.5 HU, respectively. Significant differences were observed between ∆PAs and ∆WTs, ∆PAs and ∆MTs, and ∆WTs and ∆MTs (all P < 0.001 ). Conclusion. Parotid and submandibular gland tumours have some typical clinical characteristics and CT findings, and plain and early contrast-phase CT combined with clinical parameters may be helpful for diagnosis.


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