scholarly journals An Automatic Method for Rice Seed Vigor Classification Via Radicle Emergence Testing Using Image-Processing, Curve-Fitting and Clustering Methods

2021 ◽  
Author(s):  
Damrongvudhi Onwimol ◽  
Parichat Sermwuthisarn ◽  
Wanwisa Phimcharoen ◽  
Tanapon Chaisan
Keyword(s):  
Image Processing ◽  
Curve Fitting ◽  
Direct Method ◽  
Indica Rice ◽  
Accelerated Aging ◽  
Dynamic Imaging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Clustering Methods ◽  
Radicle Emergence

Abstract Background: Rice seed vigor classification is important for seed storage management by seed producers and by farmers while planning their cultivation activities. Field emergence is a direct method of seed vigor testing but is laborious, time-consuming and subjective. The accelerated aging (AA) test is often used as an indirect method for rice seed vigor classification in the laboratory. However, the results from this method are often imprecise. This paper presents the SVRice package, a simple, cost-efficient and flexible procedure that utilizes computer image analysis for high-throughput, automatic rice seed vigor classification. SVRice consists of 4 steps: dynamic imaging, image processing, curve fitting and clustering. Seed vigor was classified based on radicle emergence indices, such as maximum radicle emergence (MaxRE), mean radicle emergence time (MRET), radicle emergence speed (t50), uniformity of radicle emergence (U7525), and area under the curve of the radicle emergence fitted curve (AUC).Results: Parameters used to classify rice seed vigor, such as MRET, U7525 and t50, were strongly negatively correlated with the saturated salt accelerated aging (SSAA) test. A germination time of 90 hours at 25°C was sufficient for effective classification based on SVRice, whereas the SSAA test took approximately 400 hours to complete. The SVRice software algorithm was created to be especially suitable for assessment after 6 months under controlled atmosphere storage (at 15°C and 37% RH in a hermetic bag). The study showed that SVRice could unambiguously classify 40 indica rice samples with different varieties, production years, production sites, storage times and storage conditions compared with the SSAA test.Conclusions: This paper confirmed the accuracy, reproducibility and flexibility of the SVRice package for automatic seed vigor classification of Oryza sativa seeds; moreover, it is also likely applicable to other species as a viable alternative to current methods that require more time and are less precise.

scholarly journals An Automatic Method for Rice Seed Vigor Classification Via Radicle Emergence Test Using Image Processing, Curve Fitting and Clustering Method

2021 ◽  
Author(s):  
Damrongvudhi Onwimol ◽  
Wanwisa Phimcharoen ◽  
Parichat Sermwuthisarn ◽  
Sujittra Tejakhod ◽  
Tanapon Chaisan
Keyword(s):  
Image Processing ◽  
Curve Fitting ◽  
Direct Method ◽  
Indica Rice ◽  
Area Under The Curve ◽  
Dynamic Imaging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Strong Negative Correlation ◽  
Radicle Emergence

Abstract Background: Rice seed vigor classification is important for seed storage management by seed producers and by farmers planning their cultivation activities. Field emergence is a direct method of seed vigor testing but is laborious, time-consuming and subjective. The saturated salt accelerated aging (SSAA) test is often used as an indirect method for rice seed vigor classification in the laboratory. However, the results from such a method are often imprecise. This paper presents the SV-RICE package, a simple, cost-efficient and flexible procedure that utilizes computer image analysis for high-throughput, automatic rice seed vigor classification. SV-RICE consists of 4 steps: dynamic imaging, image processing, curve fitting and clustering. Seed vigor has been classified based on radicle emergence indices, such as maximum radicle emergence (MRET), mean radicle emergence time (MaxRE), radicle emergence speed (t50), uniformity of radicle emergence (U7525), and area under the curve of the radicle emergence fitted curve (AUC).Results: Parameters used to classify rice seed vigor, such as MRET, U7525 and t50, were strong negative correlation with the SSAA test. The germination time of 90 hours, at 25°C, was sufficient for effective classification based on SV-RICE, whereas the SSAA test takes approximately 400 hours to complete. The SV-RICE software algorithm was set up to be especially suitable for assessment after 6 months under controlled atmosphere storage (at 15°C and 37%RH in hermetic bag). The study showed that SV-RICE could unambiguously classify 40 Indica rice samples with different varieties, production years, production sites, storage times and storage conditions compared to the SSAA test.Conclusions: This paper confirmed the accuracy, reproducibility and flexibility of the SV-RICE package for automatic seed vigor classification of Oryza sativa seeds; however, it is likely applicable to other species as a viable alternative to current methods that require more time and are less precise.

scholarly journals ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

2021 ◽  
Vol 34 (4) ◽  
pp. 791-798
Author(s):  
RAFAEL MARANI BARBOSA ◽  
MATHEUS ANDRÉ DE JESUS ◽  
RAFAELA ALVES PEREIRA ◽  
GEDEON ALMEIDA GOMES JUNIOR
Keyword(s):  
Electrical Conductivity ◽  
Efficient Method ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Seed Sample ◽  
Red Rice ◽  
Germination Test ◽  
Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

scholarly journals O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ

2021 ◽  
Vol 36 (1) ◽  
pp. 28-40
Author(s):  
Jaquelini Garcia ◽  
Cileide Maria Medeiros Coelho
Keyword(s):  
Moisture Content ◽  
Seed Viability ◽  
Mechanical Damage ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Randomized Design ◽  
Physiological Quality ◽  
Drying System

O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ   JAQUELINI GARCIA1, CILEIDE MARIA MEDEIROS COELHO1   1Programa de Pós-graduação em Produção vegetal, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000, Lages, SC, Brasil, [email protected], [email protected]   RESUMO: O grau de umidade na colheita e o sistema de secagem podem ser determinantes na qualidade das sementes produzidas. Neste trabalho foi avaliado se o sistema de secagem e o grau de umidade na colheita interferem na qualidade fisiológica das sementes de arroz. Foi realizado em delineamento inteiramente casualizado em esquema fatorial triplo. Foi utilizada seis cultivares produzidas no Alto Vale do Itajaí/SC na safra 2016/17 com grau de umidade na colheita igual ou superior a 17% (19,30 - 17,30%) e inferior a 17% (16,40 - 15,03%) (base úmida). As sementes foram submetidas aos sistemas de secagem estacionário e intermitente. Realizou-se testes de germinação, viabilidade, vigor pelo envelhecimento acelerado e frio. Sementes colhidas com grau de umidade ≥ 17% apresentaram maior vigor. A cultivar foi um fator de grande relevância na sensibilidade ao dano térmico e mecânico. O sistema de secagem não afetou a viabilidade das sementes, com exceção da cultivar SCS122 Miura que foi sensível a dano térmico e mecânico. A secagem estacionária comprometeu a qualidade fisiológica. Conclui-se que a secagem intermitente e a colheita com grau de umidade igual ou superior a 17%, mantém a qualidade fisiológica das cultivares SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru e SCS121 CL.   Palavras-chaves: Secagem estacionária, secagem intermitente, qualidade fisiológica.   THE DEGREE OF MOISTURE IN THE HARVEST AND THE DRYING SYSTEM ARE DETERMINANT FOR THE RICE SEED VIGOR   ABSTRACT: The degree of moisture at harvest and the drying system can determine the quality of the seeds produced. In this work, it was evaluated whether the drying system and the degree of moisture at harvest affect the physiological quality of rice seeds. It was conducted in a completely randomized design in a triple factorial scheme. Six cultivars produced in Alto Vale do Itajaí/SC in the 2016/17 crop were used, with moisture content at harvest equal or greater than 17% (19.30 – 17.30%) and less than 17% (16,40 - 15,03%) (wet basis). Seeds were submitted to stationary and intermittent drying systems. Tests of germination, viability, vigor by accelerated aging and cold were performed. Seeds harvested with moisture content ≥ 17% showed greater vigor. The cultivar was a factor of great relevance in the sensitivity to thermal and mechanical damage. The drying system did not affect seed viability, except for the cultivar SCS122 Miura, which was sensitive to thermal and mechanical damage. Stationary drying compromised physiological quality. It was concluded that intermittent drying and harvesting with moisture content equal or greater than 17% maintain the physiological quality of the SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru and SCS121 CL cultivars.   Keywords: Stationary drying, intermittent drying, physiological quality.

scholarly journals Electrical conductivity of the seed soaking solution and soybean seedling emergence

2004 ◽  
Vol 61 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Roberval Daiton Vieira ◽  
Angelo Scappa Neto ◽  
Sonia Regina Mudrovitsch de Bittencourt ◽  
Maristela Panobianco
Keyword(s):  
Electrical Conductivity ◽  
Water Content ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Soybean Seedling ◽  
Seed Soaking ◽  
Laboratory Conditions ◽  
Field And Laboratory Conditions

Vigor of soybean [Glycine max (L.) Merrill] seeds can be evaluated by measuring the electrical conductivity (EC) of the seed soaking solution, which has shown a satisfactory relationship with field seedling emergence, but has not had aproper definition of range yet. This work studies the relationship between EC and soybean seedling emergence both in the field and laboratory conditions, using twenty two seed lots. Seed water content, standard germination and vigor (EC, accelerated aging and cold tests) were evaluated under laboratory conditions using -0.03; -0.20; -0.40 and -0.60 MPa matric potentials, and field seedling emergence was also observed. There was direct relationship between EC and field seedling emergence (FE). Under laboratory conditions, a decreasing relationship was found between EC and FE as water content in the substrate decreased. Relationships between these two parameters were also found when -0.03; -0.20 and -0.40 MPa matric potentials were used. EC tests can be used successfully to evaluate soybean seed vigor and identify lots with higher or lower field emergence potential.

scholarly journals Survey of rice seed quality analysis for varietal purity estimation by using image processing techniques

2018 ◽  
Vol 7 (1.7) ◽  
pp. 34
Author(s):  
S. Durai ◽  
C. Mahesh ◽  
T. Sujithra ◽  
A. Suresh
Keyword(s):  
Image Processing ◽  
Computer Vision ◽  
South India ◽  
Seed Quality ◽  
Quality Analysis ◽  
Rice Seed ◽  
Two Factors ◽  
Computer Vision Technology ◽  
Processing Techniques ◽  
Varietal Purity

 In south India rice is the major food source and in agriculture, rice production covers more than 70 percentages of entire forming. But in recent the production only from south India not enough to satisfy the need of all, such a huge demand is there. The better production comes from the selection of good seeds. Up to now formers depend on two factors for selecting better seeds, One is the brand which is approved by some quality standards and second one is analyzed manually by experienced people. Both are risky one, we are not pretty much sure the accuracy of analyze. The second one is seeing and feeling. The inspection is not consistent also very time consuming. In the other way we can use computer vision technology to analyze the quality of the seeds. In recent years many of the big industries they are using computer vision technology with Digital Image Processing for many of the applications. In this Paper we are going to discuss the different seed quality analyzing methods and accuracy of result also. Moreover there are different factors and features are there for it, here we are going to study about varietal purity estimation by different methods.

scholarly journals Seedling imaging analysis and traditional tests to assess okra seed vigor

2013 ◽  
Vol 35 (4) ◽  
pp. 443-448 ◽  
Author(s):  
Ana Lúcia Pereira Kikuti ◽  
Júlio Marcos-Filho
Keyword(s):  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Seed Industry ◽  
Accelerated Aging Test ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Speed

Seed vigor testing is an important component of quality control programs adopted by seed industry. The software Seed Vigor Imaging System (SVIS) has been successfully used for seed vigor assessment in different species. The objective of this research was to verify the SVIS efficiency to assess okra seed vigor in comparison to other vigor tests used for this species. Five seed lots of 'Clemson Americano' and four of 'Santa Cruz' were submitted to germination (speed and percentage), cold germination (speed and percentage), traditional and saturated salt accelerated aging (41 ºC/72 and 96 h) and seedling emergence tests during 12 months storage. Vigor index, uniformity of growth and seedling length were determined by the software Seed Vigor Imaging System (SVIS). Results showed that the vigor index and seedling length determined by the SVIS analyses are efficient to determine okra seed vigor as well as the accelerated aging test (41 ºC/72 h).

scholarly journals Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho
Keyword(s):  
Electrical Conductivity ◽  
Growth Habit ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Pea Seeds ◽  
Cold Chamber ◽  
Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

scholarly journals Pola Penurunan Viabilitas dan Pengembangan Metode Pendugaan Vigor Daya Simpan Benih Pepaya (Carica Papaya L.)

2017 ◽  
Vol 7 (3) ◽  
pp. 146
Author(s):  
Astryani Rosyad ◽  
M. Rahmad Suhartanto ◽  
Abdul Qadir
Keyword(s):  
Moisture Content ◽  
Aging Time ◽  
Seed Quality ◽  
Initial Moisture Content ◽  
Accelerated Aging ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Vigor ◽  
Develop Model ◽  
Papaya Seed

<p>ABSTRACT<br />Information of seed quality during storage can be determined through the actual storage and storability vigor estimation. This study aimed at comparing effective accelerated aging method<br />between physical and chemical, and studying the seed deterioration during storage in ambient (T =28-30 0C, RH=75-78%) and AC (T =18-20 0C, RH =51-60%) condition with three levels of initial moisture content (8-10%, 10-12%, and 12-14%) for 20 weeks. The final objective of this research<br />was to develop model for storability vigor of papaya seed. Two experiments, accelerated aging and seed storage were conducted at Seed Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University from October 2015 to May 2016. A completely randomized design with nested factors and four replications was applied to both experiments. The results showed that physical accelerated aging using IPB 77-1 MMM machine was more effective than chemical accelerated aging using IPB 77-1 MM machine for papaya seed. The viability of seed stored in AC condition remained high until the end of the storage period, whereas it declined at 16 week storage period in the ambient condition. The viability of seed with initial moisture content of 12-14% declined faster than that of initial moisture content of 8-10% after 18 week storage periode. The model used to estimate the storability vigor of papaya seed accurately was the equation y = a + b expcx where y : storability vigor estimation, x : aging time and a,b,c : constant value. Simulation of storability vigor estimation with constant value of a, b, c and input of aging time can estimate storability seed vigor in actual storage.<br />Keywords: accelerated aging, IPB 77-1 MM machine, IPB 77-1 MMM machine, seed storage, simulation</p><p>ABSTRAK<br />Informasi mutu benih selama penyimpanan dapat diketahui melalui penyimpanan secara aktual dan pendugaan vigor daya simpan. Penelitian ini bertujuan untuk membandingkan metode<br />pengusangan cepat yang efektif antara fisik dengan kimia serta mempelajari pola penurunan viabilitas benih selama penyimpanan aktual pada kondisi simpan kamar (suhu =28-30 0C, RH =75-78%) dan AC (suhu =18-20 0C, RH =51-60%) dengan tiga tingkat kadar air awal (8-10%, 10-12%, dan 12-14%) selama 20 minggu. Tujuan akhirnya adalah membangun model vigor daya simpan benih pepaya. Penelitian pengusangan cepat dan penyimpanan dilakukan pada bulan Oktober 2015 sampai Mei 2016 di Laboratorium Benih, Departemen Agronomi dan Hortikultura, Institut Pertanian<br />Bogor. Kedua penelitian menggunakan rancangan acak lengkap tersarang dengan empat ulangan. Hasil penelitian menunjukkan bahwa pengusangan cepat secara fisik dengan alat IPB 77-1 MMM lebih efektif daripada pengusangan kimia dengan alat IPB 77-1 MM untuk benih pepaya. Viabilitas benih yang disimpan pada kondisi AC tetap tinggi hingga akhir periode simpan, sedangkan pada kondisi kamar penurunan viabilitas dimulai pada periode simpan 16 minggu. Benih yang disimpan dengan tingkat KA awal sebesar 12-14% lebih cepat mengalami penurunan viabilitas mulai periode simpan 18 minggu dibandingkan dengan benih dengan KA awal 8-10%. Hasil penelitian juga menunjukkan terdapat korelasi yang erat antara pola kemunduran benih pada pengusangan cepat dan penyimpanan aktual, sehingga model pendugaan vigor daya simpan (y) berdasarkan waktu pengusangan (x) dapat disusun dengan persamaan y = a + b expcx. Simulasi pendugaan vigor daya simpan dengan nilai konstanta a, b, dan c serta input waktu pengusangan dapat menduga vigor daya simpan benih selama penyimpanan aktual.<br />Kata kunci: alat IPB 77-1 MM, alat IPB 77-1 MMM, pengusangan cepat, penyimpanan benih,<br />simulasi</p>

scholarly journals Effect of seed vigor and sowing densities on the yield and physiological potential of wheat seeds

2021 ◽  
Vol 43 ◽  
Author(s):  
Carolina Pereira Cardoso ◽  
José Henrique Bizzarri Bazzo ◽  
Jéssica de Lucena Marinho ◽  
Claudemir Zucareli
Keyword(s):  
Seed Yield ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Crop ◽  
Seedling Length ◽  
Physiological Quality ◽  
Initial Seed ◽  
Wheat Seeds

Abstract: Initial seed vigor and sowing density interact in establishment of plants in the field, and can thus affect expression of the plasticity of the wheat crop and the yield and physiological quality of the seeds produced. The aim of this study was to evaluate the effect of seed vigor levels in combination with sowing densities on the yield and physiological potential of wheat seeds. The cultivars BRS Gralha-Azul and BRS Sabiá were used in a randomized block experimental design in a 2 × 4 factorial arrangement, with four replications. The treatments consisted of seeds of high and low vigor and four sowing densities (150, 250, 350, and 450 seeds.m-2). The following evaluations were made: seed yield, germination, first germination count, seedling length, seedling dry matter, accelerated aging, emergence speed index, and seedling emergence in sand. An increase in sowing density favors the seed yield of both cultivars; however, it reduces the vigor of the seeds produced by the cultivar BRS Gralha-Azul, especially of the seeds produced by plants originating from high vigor seeds. For the cultivar BRS Sabiá, an increase in sowing density decreases the germination performance of seeds produced by plants originating from low vigor seeds.

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