scholarly journals Sero-Positivity of Japanese Encephalitis Virus in Equine Population of India Using IgG ELISA: Unraveling The Need for Vaccination

2021 ◽  
Author(s):  
Aarti Kapdi ◽  
HIMANI DHANZE ◽  
Anamika Sahu ◽  
Vijayata Singh ◽  
M. Suman Kumar ◽  
...  
Keyword(s):  
Japanese Encephalitis ◽  
Validation Studies ◽  
Horse Serum ◽  
Good Reproducibility ◽  
Ns1 Protein ◽  
Serum Samples ◽  
Fatal Disease ◽  
Kappa Value ◽  
Diagnostic Sensitivity And Specificity ◽  
First Time

Abstract Japanese encephalitis (JE) is a mosquito borne flaviviral zoonoses, causing fatal disease in equines and humans. JE is endemic in most of the states of India with occurrence of human cases every year. The horses are not vaccinated against JE in India and thus they are at more risk of acquiring the disease. Due to non-availability of indigenously developed ELISA and high cost of imported kits, regular sero-surveillance is not being carried out to assess the true picture of JE virus in equine population of India. Therefore, a recombinant NS1 protein based indirect IgG ELISA was developed with the objective to assess the sero-positivity of JE virus in equine population of India. The diagnostic sensitivity and specificity of developed ELISA was 84.73 % and 86.70 %, respectively. The validation studies revealed good reproducibility of ELISA with kappa value ranging from 0.75 to 1 between the results of different laboratories. A total of 2069 horse serum samples were screened using the developed ELISA and 401 samples were positive for IgG against JEV with an overall sero-positivity of 19.38% in equine population of India. A sero-positivity of 25.90% and 12.22% was recorded in Himachal Pradesh and Jammu-Kashmir, both hill states of North zone of India for the first time, revealing the spread of virus to the non-endemic parts of the country. The high sero-positivity of JE virus recorded in equine population warrants the need for initiation of vaccination of horses in India to prevent the morbidity and mortality.

scholarly journals Occurrence of West Nile Virus Antibodies in Wild Birds, Horses, and Humans in Poland

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Jowita Samanta Niczyporuk ◽  
Elżbieta Samorek-Salamonowicz ◽  
Sylvie Lecollinet ◽  
Sławomir Andrzej Pancewicz ◽  
Wojciech Kozdruń ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Japanese Encephalitis ◽  
Wild Bird ◽  
Horse Serum ◽  
Wild Birds ◽  
Serum Samples ◽  
West Nile ◽  
Cross Reactions ◽  
Lymphocytic Meningitis ◽  
Positive Results

Serum samples of 474 wild birds, 378 horses, and 42 humans with meningitis and lymphocytic meningitis were collected between 2010 and 2014 from different areas of Poland. West Nile virus (WNV) antibodies were detected using competition enzyme linked immunosorbent assays: ELISA-1 ID Screen West Nile Competition, IDvet, ELISA-2 ID Screen West Nile IgM Capture, and ELISA-3 Ingezim West Nile Compac. The antibodies were found in 63 (13.29%) out of 474 wild bird serum samples and in one (0.26%) out of 378 horse serum samples. Fourteen (33.33%) out of 42 sera from patients were positive against WNV antigen and one serum was doubtful. Positive samples obtained in birds were next retested with virus microneutralisation test to confirm positive results and cross-reactions with other antigens of the Japanese encephalitis complex. We suspect that positive serological results in humans, birds, and horses indicate that WNV can be somehow closely related with the ecosystem in Poland.

scholarly journals Serum Neutralization Profiles of Straw-Colored Fruit Bats (Eidolon helvum) in Makurdi (Nigeria), against Four Lineages of Lagos Bat Lyssavirus

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2378
Author(s):  
Veronica Odinya Ameh ◽  
Guanghui Wu ◽  
Hooman Goharriz ◽  
Rebecca Shipley ◽  
Anthony R. Fooks ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Fluorescent Antibody ◽  
Virus Neutralization ◽  
Human Consumption ◽  
Fruit Bats ◽  
Serum Samples ◽  
Fatal Disease ◽  
Serum Neutralization ◽  
First Time ◽  
Eidolon Helvum

Lagos bat lyssavirus (LBV) comprising four lineages (A, B, C and D) can potentially cause the fatal disease rabies. Although LBV-B was initially isolated in Nigeria in 1956, there is no information on LBV lineages circulating in Nigeria. This study was undertaken for the first time to measure the neutralizing antibodies against four lineages of LBVs in straw-colored fruit bats (Eidolon helvum) in Makurdi, Nigeria. Serum samples (n = 180) collected during two periods (November 2017–March 2018 and November 2018–March 2019) from terminally bled bats captured for human consumption were tested using a modified fluorescent antibody virus neutralization (mFAVN) assay. A high proportion of bat sera (74%) neutralized at least one lineage of LBV (with reciprocal titers from 9 to >420.89) and most of them neutralized LBV-A (63%), followed by LBV-D (49%), LBV-C (45%) and LBV-B (24%). The majority of positive sera (75%, n = 100) neutralized multiple LBV lineages while the remaining 25% (n = 33) neutralized only a single lineage, i.e., LBV-A (n = 23), LBV-D (n = 8) and LBV-C (n = 2). None exclusively neutralized LBV-B. The results suggest that exposure to LBV is common in E. helvum and that LBV-A (but not LBV-B) is likely to be circulating in this region of Nigeria.

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

scholarly journals Recognition Pattern of the Fasciola hepatica Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 725
Author(s):  
David Becerro-Recio ◽  
Javier González-Miguel ◽  
Alberto Ucero ◽  
Javier Sotillo ◽  
Álvaro Martínez-Moreno ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Fasciola Hepatica ◽  
Cathepsin L ◽  
Helminth Parasites ◽  
Glutathione S Transferase ◽  
Serum Samples ◽  
Secretory Products ◽  
Immunogenic Proteins ◽  
Host Parasite ◽  
First Time

Excretory/secretory products released by helminth parasites have been widely studied for their diagnostic utility, immunomodulatory properties, as well as for their use as vaccines. Due to their location at the host/parasite interface, the characterization of parasite secretions is important to unravel the molecular interactions governing the relationships between helminth parasites and their hosts. In this study, the excretory/secretory products from adult worms of the trematode Fasciola hepatica (FhES) were employed in a combination of two-dimensional electrophoresis, immunoblot and mass spectrometry, to analyze the immune response elicited in sheep during the course of an experimental infection. Ten different immunogenic proteins from FhES recognized by serum samples from infected sheep at 4, 8, and/or 12 weeks post-infection were identified. Among these, different isoforms of cathepsin L and B, peroxiredoxin, calmodulin, or glutathione S-transferase were recognized from the beginning to the end of the experimental infection, suggesting their potential role as immunomodulatory antigens. Furthermore, four FhES proteins (C2H2-type domain-containing protein, ferritin, superoxide dismutase, and globin-3) were identified for the first time as non-immunogenic proteins. These results may help to further understand host/parasite relationships in fasciolosis, and to identify potential diagnostic molecules and drug target candidates of F. hepatica.

Protective immunity of E. coli-synthesized NS1 protein of Japanese encephalitis virus

2007 ◽  
Vol 30 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Cheng-Wen Lin ◽  
Kuang-Ting Liu ◽  
Hong-Da Huang ◽  
Wei-June Chen
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Protective Immunity ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
E Coli

scholarly journals Bovine ephemeral fever epidemics in Kingdom Saudi Arabia: clinical, epidemiological and molecular investigation

2017 ◽  
Vol 11 (11) ◽  
pp. 854-860 ◽  
Author(s):  
Ahmed A Zaghawa ◽  
Fadhel Housawi ◽  
Abdulmohsen Al-Naeem ◽  
Ahmed Elsify ◽  
Yamen Mohammed Hegazy
Keyword(s):  
Saudi Arabia ◽  
Water Buffalo ◽  
Virus Isolation ◽  
Clinical Symptoms ◽  
Rt Pcr ◽  
Serum Samples ◽  
Bovine Ephemeral Fever ◽  
Geographical Regions ◽  
Epidemiological Pattern ◽  
First Time

Introduction: Bovine ephemeral fever virus (BEFV) is an arthropod borne Rhabdovirus affects cattle and water buffalo causes acute febrile disease. Methodology: The clinical picture and epidemiological pattern of BEF were described among cattle in epidemics of 2007, 2009 and 2011 in four geographical regions of Kingdom Saudi Arabia (Eastern, Jizan, Qasim, and Riyadh). Serum samples were tested using VNT. Virus isolation and molecular characterization were carried out for the first time in KSA. Results: The main clinical symptoms were fever, stiffness, lameness, salivation and subcutaneous emphysema. The prevalence and the mortality rate of BEF have decreased from 70% and 4.6% in 2007 to 30% and 0.6% in 2011, respectively in the 4 studied areas. There was no region association with higher prevalence of BEF. The intracluster correlation (ICC) was estimated for the first time in KSA as 0.0034. BEFV had been isolated from 11 out of 20 samples (55%) and isolation was confirmed by VNT. The molecular detection of BEFV by RT-PCR and real- time RT-qPCR were found more sensitive for diagnosis of the disease than virus isolation; 80% and 90% for the former tests and 55% for the latter. Three isolates were sequenced, they showed 84.7% - 100% identities in between and shared 90.4%-96.5% sequence identity with a previously published sequence from Australia (KF679404). The generated sequences belonged to 3rd cluster of BEFV glycoprotein. Conclusions: BEF occurrence has cyclic nature and the efficacy of vaccines prepared from local strains has to be evaluated and considered in diseases control.

scholarly journals Botulism (type A) in a horse - case report

2016 ◽  
Vol 85 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Sevim Kasap ◽  
Hasan Batmaz ◽  
Meric Kocaturk ◽  
Frank Gessler ◽  
Serkan Catık ◽  
...  
Keyword(s):  
Botulinum Neurotoxin ◽  
Clinical Signs ◽  
Type A ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Thoroughbred Horse ◽  
Botulinum Neurotoxin Type A ◽  
Botulinum Neurotoxins ◽  
First Time ◽  
Colonic Content

This paper presents the case of a six year-old, male, thoroughbred horse with clinical signs of inappetence, weakness, and incoordination when walking. Clinical examination showed that the horse staggered and leaned to the left side. Feedstuff was present inside and around its mouth. Salivation was increased and there was no reflex at the palpebrae and tongue. The horse had difficulty swallowing and the tone of its tail was reduced. Botulism was diagnosed based on the clinical signs. Antibiotic (ceftiofur) and fluid-electrolyte treatment was commenced. Next day, neostigmin was added to the horse’s treatment, and it became recumbent. The horse’s palpebral, tongue and tail reflexes returned partially after neostigmine methylsulphate treatment on the same day and it stood up on day four. However, it could not swallow anything during the whole week, so after getting permission from the owner, the horse was euthanized on day 10. Samples of the colonic content and blood serum were sent by courier to the laboratory for toxin neutralization, however, botulinum neurotoxins could not be detected. After that, serum samples from days 6 and 10 were sent to another laboratory for testing for botulinum neurotoxin antibodies by ELISA. Specific antibodies against botulinum neurotoxin type A were measured, indicating a previous, immuno-relevant contact with the toxin. This seroconversion for type A supports the clinical botulism diagnosis. Type A botulism is rarely seen in Europe and has been detected in a horse in Turkey for the first time.

scholarly journals A STUDY ON THE PREVALENCE AND SEASONAL VARIATION OF HEPATITIS A IN PATIENTS ATTENDING JNIMS HOSPITAL

2018 ◽  
pp. 1-2
Author(s):  
Dr. Dayananda Ingudam
Keyword(s):  
Seasonal Variation ◽  
Viral Hepatitis ◽  
Liver Dysfunction ◽  
Rainy Season ◽  
Hepatitis A ◽  
Acute Viral Hepatitis ◽  
Developing Nations ◽  
Serum Samples ◽  
Fatal Disease ◽  
Common Cause

BACKGROUND: Hepatitis is the most common cause of acute viral hepatitis. It causes severe, often fatal disease in adults especially in those with underlying liver dysfunction but is mostly asymptomatic in children. The present study was conducted with the aimed to nd the prevalence and seasonal variation of HAVinfection in patients attending JNIMS hospital. METHODOLOGY:Across sectional hospital based study was conducted in the department of Microbiology, JNIMS, Imphal, Manipur. Rapid immunochromatographic assay was performed for the detection of IgM antibody to HAVin human serum. RESULTS: Out of the 84 serum samples 20(23.80%) was found to be positive for hepatitis IgM, of which 11 (55%) were found in males and 9(45%) were found in females. A seasonal increase in incidence of HAV infection was found during the rainy season of January – March (50%) and April – June (35%). CONCLUSION: The global seroepidemiology of hepatitis A is changing in many developing nations giving rise to unique non immune adolescent and adult populations emerging in endemic regions thereby conferring a risk of developing severe hepatitis A. Hence, nding the prevalence, seasonal variation and timely detection of HAVinfection will help in minimising the development of further complication

scholarly journals The Japanese Encephalitis Virus NS1′ Protein Inhibits Type I IFN Production by Targeting MAVS

2020 ◽  
Vol 204 (5) ◽  
pp. 1287-1298 ◽  
Author(s):  
Dengyuan Zhou ◽  
Qiuyan Li ◽  
Fan Jia ◽  
Luping Zhang ◽  
Shengfeng Wan ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Type I ◽  
Type I Ifn ◽  
Ns1 Protein
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