scholarly journals Differentially directional selection on grain size genes identified by RapMap during rice domestication

2020 ◽  

Abstract The authors have requested that this preprint be withdrawn due to erroneous posting.

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Juncheng Zhang ◽  
Dejian Zhang ◽  
Yawei Fan ◽  
Cuicui Li ◽  
Pengkun Xu ◽  
...  

AbstractCloning quantitative trait locus (QTL) is time consuming and laborious, which hinders the understanding of natural variation and genetic diversity. Here, we introduce RapMap, a method for rapid multi-QTL mapping by employing F2 gradient populations (F2GPs) constructed by minor-phenotypic-difference accessions. The co-segregation standard of the single-locus genetic models ensures simultaneous integration of a three-in-one framework in RapMap i.e. detecting a real QTL, confirming its effect, and obtaining its near-isogenic line-like line (NIL-LL). We demonstrate the feasibility of RapMap by cloning eight rice grain-size genes using 15 F2GPs in three years. These genes explain a total of 75% of grain shape variation. Allele frequency analysis of these genes using a large germplasm collection reveals directional selection of the slender and long grains in indica rice domestication. In addition, major grain-size genes have been strongly selected during rice domestication. We think application of RapMap in crops will accelerate gene discovery and genomic breeding.


2020 ◽  
Author(s):  
Yibo Li ◽  
Juncheng Zhang ◽  
Dejian Zhang ◽  
Yawei Fan ◽  
Cuicui Li ◽  
...  

Abstract The ability to identify quantitative trait locus (QTL) genes controlling natural variation of important traits would facilitate the construction of a bridge between gene function analyses and domestication investigations. Grain size in rice is an important yield, quality and domestication trait. The molecular architecture and domestication signatures of grain size at population level remain unknown. Here we first introduce RapMap, a method for rapid and high-throughput QTL mapping employing a series of F2 gradient populations (F2GPs) constructed by trait-adjacent accessions from diverse germplasms. A co-segregation standard is set for simultaneously integrating the three-in-one framework in RapMap: detecting a real QTL, confirming its effect and obtaining its near-isogenic line-like line (NIL-LL), which greatly enhances the efficiency and robustness of QTL identification. Using 15 gradient populations, eight genes including two novel genes (GL1 and GW5.1) with different effects for grain size in rice were simultaneously discovered by RapMap in three years, which make more than two thirds of genetic contributions to grain size and shape in a mini-core collection worldwide. In-depth analyses of the eight genes employing a large and geographically diverse population, including 446 wild, 2462 landrace and 784 cultivar accessions, revealed that directional artificial selection of slender- and long- grains and their alleles played an important role during indica rice domestication. The genetic effects of the eight grain-size genes positively correlate with the differential selection intensities and the nucleotide variation intensities. Major grain-size genes fixed before the completion of rice domestication have been heavily selected for human requirements and cultivation practices. Generalizable application of the method and mimicking of the domestication nature will accelerate breeding modern cultivars.


2008 ◽  
Vol 40 (8) ◽  
pp. 1023-1028 ◽  
Author(s):  
Ayahiko Shomura ◽  
Takeshi Izawa ◽  
Kaworu Ebana ◽  
Takeshi Ebitani ◽  
Hiromi Kanegae ◽  
...  

Author(s):  
K. P. Staudhammer ◽  
L. E. Murr

The effect of shock loading on a variety of steels has been reviewed recently by Leslie. It is generally observed that significant changes in microstructure and microhardness are produced by explosive shock deformation. While the effect of shock loading on austenitic, ferritic, martensitic, and pearlitic structures has been investigated, there have been no systematic studies of the shock-loading of microduplex structures.In the current investigation, the shock-loading response of millrolled and heat-treated Uniloy 326 (thickness 60 mil) having a residual grain size of 1 to 2μ before shock loading was studied. Uniloy 326 is a two phase (microduplex) alloy consisting of 30% austenite (γ) in a ferrite (α) matrix; with the composition.3% Ti, 1% Mn, .6% Si,.05% C, 6% Ni, 26% Cr, balance Fe.


Author(s):  
R. Sinclair ◽  
B.E. Jacobson

INTRODUCTIONThe prospect of performing chemical analysis of thin specimens at any desired level of resolution is particularly appealing to the materials scientist. Commercial TEM-based systems are now available which virtually provide this capability. The purpose of this contribution is to illustrate its application to problems which would have been intractable until recently, pointing out some current limitations.X-RAY ANALYSISIn an attempt to fabricate superconducting materials with high critical currents and temperature, thin Nb3Sn films have been prepared by electron beam vapor deposition [1]. Fine-grain size material is desirable which may be achieved by codeposition with small amounts of Al2O3 . Figure 1 shows the STEM microstructure, with large (∽ 200 Å dia) voids present at the grain boundaries. Higher quality TEM micrographs (e.g. fig. 2) reveal the presence of small voids within the grains which are absent in pure Nb3Sn prepared under identical conditions. The X-ray spectrum from large (∽ lμ dia) or small (∽100 Ǻ dia) areas within the grains indicates only small amounts of A1 (fig.3).


Author(s):  
Takao Suzuki ◽  
Hossein Nuri

For future high density magneto-optical recording materials, a Bi-substituted garnet film ((BiDy)3(FeGa)5O12) is an attractive candidate since it has strong magneto-optic effect at short wavelengths less than 600 nm. The signal in read back performance at 500 nm using a garnet film can be an order of magnitude higher than a current rare earth-transition metal amorphous film. However, the granularity and surface roughness of such crystalline garnet films are the key to control for minimizing media noise.We have demonstrated a new technique to fabricate a garnet film which has much smaller grain size and smoother surfaces than those annealed in a conventional oven. This method employs a high ramp-up rate annealing (Γ = 50 ~ 100 C/s) in nitrogen atmosphere. Fig.1 shows a typical microstruture of a Bi-susbtituted garnet film deposited by r.f. sputtering and then subsequently crystallized by a rapid thermal annealing technique at Γ = 50 C/s at 650 °C for 2 min. The structure is a single phase of garnet, and a grain size is about 300A.


Author(s):  
Ernest L. Hall ◽  
Shyh-Chin Huang

Addition of interstitial elements to γ-TiAl alloys is currently being explored as a method for improving the properties of these alloys. Previous work in which a number of interstitial elements were studied showed that boron was particularly effective in refining the grain size in castings, and led to enhanced strength while maintaining reasonable ductility. Other investigators have shown that B in γ-TiAl alloys tends to promote the formation of TiB2 as a second phase. In this study, the microstructure of Bcontaining TiAl alloys was examined in detail in order to describe the mechanism by which B alters the structure and properties of these alloys.


Author(s):  
Ernest L. Hall ◽  
Lee E. Rumaner ◽  
Mark G. Benz

The intermetallic compound Nb3Sn is a type-II superconductor of interest because it has high values of critical current density Jc in high magnetic fields. One method of forming this compound involves diffusion of Sn into Nb foil containing small amounts of Zr and O. In order to maintain high values of Jc, it is important to keep the grain size in the Nb3Sn as small as possible, since the grain boundaries act as flux-pinning sites. It has been known for many years that Zr and O were essential to grain size control in this process. In previous work, we have shown that (a) the Sn is transported to the Nb3Sn/Nb interface by liquid diffusion along grain boundaries; (b) the Zr and O form small ZrO2 particles in the Nb3Sn grains; and (c) many very small Nb3Sn grains nucleate from a single Nb grain at the reaction interface. In this paper we report the results of detailed studies of the Nb3Sn/Nb3Sn, Nb3Sn/Nb, and Nb3Sn/ZrO2 interfaces.


Author(s):  
J.M. Schwartz ◽  
L.F. Francis ◽  
L.D. Schmidt ◽  
P.S. Schabes-Retchkiman

Ceramic thin films and coatings are of interest for electrical, optical, magnetic and thermal barrier applications. Critical for improved properties in thin films is the development of specific microstructures during processing. To this end, the sol-gel method is advantageous as a versatile processing route. The sol-gel process involves depositing a solution containing metalorganic or colloidal ceramic precursors onto a substrate and heating the deposited layer to form a crystalline or non-crystalline ceramic coating. This route has several advantages, including the ability to create tailored microstructures and properties, to coat large or small areas, simple or complex shapes, and to more easily prepare multicomponent ceramics. Sol-gel derived coatings are amorphous in the as-deposited state and develop their crystalline structure and microstructure during heat-treatment. We are particularly interested in studying the amorphous to crystalline transformation, because many key features of the microstructure such as grain size and grain size distribution may be linked to this transformation.


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