Biochar enhanced the nitrogen removal performance of heterotrophic nitrification-aerobic denitrification strain pseudomonas fluorescent sp. Z03 for insufficient carbon source wastewater under low temperature

Abstract In this study, biochar BC400 and BC700 were prepared, characterized and coupled with heterotrophic nitrification-aerobic denitrification (HNAD) strain Z03 for nitrogen removal experiments. The characterization results showed that BC700 has a higher specific surface area and a more complex multilayered pore structure, with increased aromatic condensation and higher crystallinity. BC400 and BC700 both have good redox activity, while BC400 has stronger electron donor capacities and BC700 owns better electron transfer properties. In addition, both BC400 and BC700 contain relatively high levels of dissolved organic carbon (DOC), reaching at 62.95 and 51.617mg/g respectively. BC400/BC700 coupled with strain Z03 can significantly improve the NH4+-N removal performance of low-temperature and low C/N wastewater compared with the control group. At a dosage of 4.0 g/L, the removal rate of NH4+-N reached to 95.16% (BC400 + Z03) and 84.37% (BC700 + Z03) within 72h, respectively. Higher than the sum of adsorption by BC400/BC700 (16.19%/18.85%) and microbial degradation (41.03%). Besides, the BC400 + BC700 + Z03 NH4+-N removal systems provide higher nitrogen removal efficiencies than BC400/BC700 + Z03 nitrogen removal systems. When the dosage (BC400 + BC700, mass ratio 5:1) reaches 3.0g/L, it can achieve more than 90% NH4+-N removal rate within 48h. The reasons for the promotion of biochar on microbial denitrification were analyzed as follows: 1) DOC can provide an additional carbon source for microorganisms; 2) biochar, as a pH buffer, can neutralize the acidity due to nitrification; 3) BC400 and BC700, as materials with good redox activity, may play a role in promoting the activity of electron transfer system and enzyme activity.

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Nitrogen Removal Characteristics of Pseudomonas putida Y-9 Capable of Heterotrophic Nitrification and Aerobic Denitrification at Low Temperature

BioMed Research International ◽

10.1155/2017/1429018 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 11

Author(s):

Yi Xu ◽

Tengxia He ◽

Zhenlun Li ◽

Qing Ye ◽

Yanli Chen ◽

...

Keyword(s):

Low Temperature ◽

Pseudomonas Putida ◽

Nitrogen Removal ◽

Removal Rate ◽

Heterotrophic Nitrification ◽

Oxidation Products ◽

Aerobic Denitrification ◽

Nitrification Rate ◽

High Concentration ◽

Nitrate And Nitrite

The cold-adapted bacterium Pseudomonas putida Y-9 was investigated and exhibited excellent capability for nitrogen removal at 15°C. The strain capable of heterotrophic nitrification and aerobic denitrification could efficiently remove ammonium, nitrate, and nitrite at an average removal rate of 2.85 mg, 1.60 mg, and 1.83 mg NL−1 h−1, respectively. Strain Y-9 performed nitrification in preference to denitrification when ammonium and nitrate or ammonium and nitrite coexisted in the solution. Meantime, the presence of nitrate had no effect on the ammonium removal rate of strain Y-9, and yet the presence of high concentration of nitrite would inhibit the cell growth and decrease the nitrification rate. The experimental results indicate that P. putida Y-9 has potential application for the treatment of wastewater containing high concentrations of ammonium along with its oxidation products at low temperature.

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Identification and Characterization of Janthinobacterium svalbardensis F19, a Novel Low-C/N-Tolerant Denitrifying Bacterium

Applied Sciences ◽

10.3390/app9091937 ◽

2019 ◽

Vol 9 (9) ◽

pp. 1937 ◽

Cited By ~ 5

Author(s):

Yinyan Chen ◽

Peng Jin ◽

Zhiwen Cui ◽

Tao Xu ◽

Ruojin Zhao ◽

...

Keyword(s):

Nitrogen Removal ◽

Removal Rate ◽

Pcr Amplification ◽

Domestic Wastewater ◽

Heterotrophic Nitrification ◽

Aerobic Denitrification ◽

Nitrite Accumulation ◽

Specific Pcr ◽

Domestic Wastewater Treatment ◽

Initial Ph

Herein, we isolated Janthinobacterium svalbardensis F19 from sludge sediment. Strain F19 can simultaneously execute heterotrophic nitrification and aerobic denitrification under aerobic conditions. The organism exhibited efficient nitrogen removal at a C/N ratio of 2:1, with an average removal rate of 0.88 mg/L/h, without nitrite accumulation. At a C/N ratio of 2, an initial pH of 10.0, a culturing temperature of 25 °C, and sodium acetate as the carbon source, the removal efficiencies of ammonium, nitrate, nitrite, and hydroxylamine were 96.44%, 92.32%, 97.46%, and 96.69%, respectively. The maximum removal rates for domestic wastewater treatment for ammonia and total nitrogen were 98.22% and 92.49%, respectively. Gene-specific PCR amplification further confirmed the presence of napA, hao, and nirS genes, which may contribute to the heterotrophic nitrification and aerobic denitrification capacity of strain F19. These results indicate that this bacterium has potential for efficient nitrogen removal at low C/N ratios from domestic wastewater.

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Simultaneous heterotrophic nitrification and aerobic denitrification at high concentrations of NaCl and ammonia nitrogen by Halomonas bacteria

Water Science & Technology ◽

10.2166/wst.2017.214 ◽

2017 ◽

Vol 76 (2) ◽

pp. 386-395 ◽

Cited By ~ 9

Author(s):

Te Wang ◽

Jian Li ◽

Ling Hua Zhang ◽

Ying Yu ◽

Yi Min Zhu

Keyword(s):

Removal Rate ◽

Sodium Succinate ◽

Bacterial Genome ◽

Ammonia Nitrogen ◽

Compatible Solute ◽

Heterotrophic Nitrification ◽

Aerobic Denitrification ◽

Sequencing Analysis ◽

N Removal ◽

High Concentrations

To improve the efficiency of simultaneous heterotrophic nitrification and aerobic denitrification (SND) at high concentrations of NaCl and ammonia nitrogen (NH4+—N), we investigated the SND characteristics of Halomonas bacteria with the ability to synthesize the compatible solute ectoine. Halomonas sp. strain B01, which was isolated, screened and identified in this study, could simultaneously remove nitrogen (N) by SND and synthesize ectoine under high NaCl conditions. Gene cloning and sequencing analysis indicated that this bacterial genome contains ammonia monooxygenase (amoA) and nitrate reductase (narH) genes. Optimal conditions for N removal in a solution containing 600 mg/L NH4+–N were as follows: sodium succinate supplied as organic carbon (C) source at a C/N ratio of 5, pH 8 and shaking culture at 90 rpm. The N removal rate was 96.0% under these conditions. The SND by Halomonas sp. strain B01 was performed in N removal medium containing 60 g/L NaCl and 4,000 mg/L NH4+–N; after 180 h the residual total inorganic N concentration was 21.7 mg/L and the N removal rate was 99.2%. Halomonas sp. strain B01, with the ability to synthesize the compatible solute ectoine, could simultaneously tolerate high concentrations of NaCl and NH4+–N and efficiently perform N removal by SND.

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Isolation and Characteristics of Heterotrophic Nitrification-Aerobic Denitrification Bacterium, Bacillus cereus X7 at High Salinity

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.864-867.111 ◽

2013 ◽

Vol 864-867 ◽

pp. 111-114 ◽

Cited By ~ 2

Author(s):

Ru Lei Yao ◽

Li Na Qiu ◽

Wei Wei Zhang ◽

Ai Jun Gong ◽

Zi Yu Wang ◽

...

Keyword(s):

Bacillus Cereus ◽

Nitrogen Removal ◽

High Salinity ◽

Removal Rate ◽

Aerobic Denitrification ◽

16S Rdna Sequence ◽

16S Rdna Sequence Analysis ◽

N Removal ◽

Nacl Concentration ◽

Biological Nitrogen

Biological nitrogen removal has been focused on in wastewater treatment field recently. A strain X7 was isolated from the sediment of pickle foodstuff wastewater. Based on its 16S rDNA sequence analysis, X7 was identified as Bacillus cereus. At NaCl concentration of 20 g/L, NH4+-N removal rate achieved 99.18%, when NO2--N and NO3--N removal rates were 77.24% and 68.6%, respectively. When NaCl concentration ranged from 0 to 40 g/L, the removal rate of NH4+-N was more than 97.59%. Therefore, due to the high nitrogen removal rate and excellent salt tolerance, Bacillus cereus X7 had a broad application prospect in the biodenitrification of brine wastewater.

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A Study on the Start-up Period of Underground Infiltration System Treating Rural Domestic Sewage

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.774-776.552 ◽

2013 ◽

Vol 774-776 ◽

pp. 552-555

Author(s):

Ming Fen Niu ◽

Jian Wei ◽

Jian Ma ◽

Xin Chen

Keyword(s):

Low Temperature ◽

Carbon Source ◽

Nitrogen Removal ◽

Nitrate Nitrogen ◽

Removal Rate ◽

Nitrogen Concentration ◽

Domestic Sewage ◽

Nitrogen And Phosphorus ◽

Start Up ◽

Better Than

This paper studied the removal effect of domestic sewage nitrogen and phosphorus during the start-up period of underground infiltration system. The experiment is conducted under the conditions of influent temperature 9~10°C, hydraulic loading 0.04m3·m-2·d-1, COD concentration 112.31~143.49mg / L, NH4+-N 20.93~27.26mg / L and TP 2.12~2.86mg / L. The experimental results showed the removal rate of COD, NH4+-N and TP was 80%, 98% and 95% respectively. In the start-up period, TN removal was not obviously for 34% removal rate. After treated the effluent nitrate nitrogen concentration was high, showing during the start-up period the nitrification was better than the denitrification. Lacking of carbon source and low temperature may account for this. To improve the conditions of the start-up period, promoting denitrification was the key to improve nitrogen removal rate by underground infiltration system.

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Regulating Strategies of Strengthening Nitrogen Removal for Low-Carbon Wastewater in Reversed A2/O Process

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.243-249.4811 ◽

2011 ◽

Vol 243-249 ◽

pp. 4811-4816 ◽

Cited By ~ 1

Author(s):

Jie Yun Chen ◽

Zhi Zhang ◽

Li Hua Xie ◽

Ling Kong ◽

Xiao Jing Yin

Keyword(s):

Low Temperature ◽

Carbon Source ◽

Nitrogen Removal ◽

Removal Rate ◽

Experimental Studies ◽

Reflux Ratio ◽

Low Carbon ◽

Efficient Operation ◽

Anoxic Environment ◽

Aerobic Zone

According to the ineffective denitrification and the initial controlling technique flaws for low-carbon source in reversed A2/O process, and full scale experimental studies were conducted to strengthen denitrification at normal and low temperature days in 2008~2010, to ensure the best conditions of biological denitrification and promote the stable and efficient operation of the system. The results showed that in normal temperature seasons of 2008, after shortening the HRT of the primary sedimentation tank to 1/3 of design value, improving the MLSS to 4500mg/L, setting the first section of the aerobic zone as the denitrification transition section and improving the reflux ratio, available carbon source was increased by 15%, the effluent NH3-N was 2.5mg / L and the removal rate was 90%, the effluent TN was 17 mg/L and the removal rate was increased to 54% and unit power consumption was reduced by 15% to 0.22Kw•h/m3.At low temperature seasons of 2008~2009,by improving the MLSS to 6000mg/L, extending the sludge age to enrich a large number of nitrification and denitrification bacteria, controlling DO at about 1.2mg / L in the aerobic zone and so on, the effluent NH3-N was 3 mg / L and the removal rate was 88%, the effluent TN was 15.5 mg/L and the removal rate was 62%. In low temperature of 2009 ~2010, besides the same measures as before, adding a blender in the first section of the aerobic zone to provide a better anoxic environment for denitrification and to improve nitrogen removal, the effluent NH3-N was 3 mg / L and the removal rate was 87%, the effluent TN was 13.5 mg/L and the removal rate was 66%.

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