scholarly journals Synergistic Cytotoxic Effect of Honey bee venom and Cisplatin on Tongue Squamous Cell Carcinoma

2021 ◽  
Author(s):  
Sabreen Gamal Khalil ◽  
Amr Helmy Mustafa El-Bolok ◽  
Sherif Farouk El-Gayar ◽  
Maii Ibrahim Sholkamy
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Honey Bee ◽  
Mtt Assay ◽  
Microscopic Examination ◽  
Cytotoxic Effect ◽  
Caspase 3 ◽  
Rt Pcr ◽  
Anti Cancer ◽  
Synergistic Cytotoxic Effect

Abstract Background: Cancer is a serious issue that has a significant effect on the health of all human communities. Tongue cancer is one of the most common head and neck cancers in the world. In the medical world, cancer therapy is a major challenge. Nowadays, natural compounds are important resources of many anti-cancer medications. Venom from honey bees possesses potent anti-cancer effects. Cisplatin is a chemotherapeutic drug that has been used for decades to treat cancer cells. Recently, Combination therapy has been a popular treatment choice for cancer patients.This study aimed to investigate the synergistic cytotoxic effect of honey bee venom (BV) and cisplatin on tongue squamous cell carcinoma cell line (SCC-25).Methods: The cytotoxic effect was determined using Methyl Thiazol Tetrazolium (MTT) assay, microscopic examination, P53 and caspase-3 were quantified by Real-Time Polymerase chain reaction (RT-PCR) and statistical analysis.Results: The findings revealed that the tested drugs' cytotoxic potential against SCC-25 cells is dose dependent. The half-maximal inhibitory concentration (IC50) value of MTT assay of BV/cisplatin mix decreased significantly compared to IC50 values of BV and cisplatin in sole formulation. Microscopic examination showed that BV and cisplatin alone and in combination mainly produced apoptotic cell death. Regarding RT-PCR results, P53 and caspase-3 expression were significantly increased in SCC-25-treated cells (P= 0.0001).Conclusions: The combined use of BV and cisplatin induced marked synergistic cytotoxic effect on SCC-25 cell line.

scholarly journals Synergistic Cytotoxic Effect of Honey Bee Venom and Cisplatin on Tongue Squamous Cell Carcinoma Cell Line

2021 ◽  
Vol 9 (B) ◽  
pp. 1739-1744
Author(s):  
Sabreen Amar ◽  
Amr Helmy Mustafa El-Bolok ◽  
Sherif Farouk El-Gayar ◽  
Maii Ibrahim Sholkamy
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Honey Bee ◽  
Squamous Cell ◽  
Microscopic Examination ◽  
Cytotoxic Effect ◽  
Rt Pcr ◽  
Anti Cancer ◽  
Synergistic Cytotoxic Effect

BACKGROUND: Tongue cancer is one of the most common head and neck cancers in the world. Nowadays, natural compounds are important resources of many anti-cancer drugs. Venom from honey bees possesses potent anti-cancer activities. Cisplatin is a chemotherapeutic drug that has been used for decades to treat cancer cells. Recently, combination therapy has been a popular treatment choice for cancer patients. AIM: This study was conducted to evaluate the synergistic cytotoxic effect of honey bee venom (BV) and cisplatin on tongue squamous cell carcinoma 25 (SCC-25) cell lines. METHODS: The cytotoxic effect was determined using methyl thiazol tetrazolium assay, microscopic examination, real-time polymerase chain reaction (RT-PCR), and statistical analysis. RESULTS: The findings revealed that the cytotoxic potential of the tested drugs on SCC-25 cells was dose-dependent. Microscopic examination showed that BV and cisplatin alone and in combination mainly produced apoptotic cell death. Regarding RT-PCR results, P53 and caspase-3 expression levels were significantly increased in SCC-25-treated cells (p = 0.0001). CONCLUSION: The combined use of BV and cisplatin induced a marked synergistic cytotoxic effect on SCC-25 cell line.

scholarly journals Anti-Cancer Activity Of Luvunga Scandens Extract Against Squamous Cell Carcinoma

2017 ◽  
Vol 16 (2) ◽  
Author(s):  
Sama Naziyah Shaban ◽  
Solachuddin Icwan ◽  
Muhamamd Taher Bakhtiar
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Caspase 3 ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Cell Cycle Analysis ◽  
Squamous Carcinoma Cells ◽  
Anti Cancer

Introduction: Squamous cell carcinoma is reported as one of the most common types of cancer with increasing numbers of occurrence. Luvunga scandens is a plant possessing many bioactivities and general health effects, yet its anti-proliferative effect is under reported and need to be scientifically evaluated. Materials and Methods: MTT assay was used to assess the cytotoxicity of the plant against human squamous carcinoma cells in addition to the safety assessment for human dermal fibroblast cell line (HDF). The morphological changes of L. scandens treated squamous carcinoma cells has been confirmed by SEM, the apoptosis of the plant against squamous carcinoma cells has been tested using caspase 3/7 assay, followed by cell cycle analysis done using a flowcytometer on squamous carcinoma cells treated with the IC50 dose of L. scandens plant. Results: The plant's extract possesses cytotoxic effect against squamous carcinoma cells with IC50 readings; (methanol= 37.5 mg/mL, dichloromethane= 38 mg/mL, hexane= 37.5 mg/mL), and safe on HDF cells. The SEM results demonstrate that L. scandens treated cells showed an overall change in the cell shape, alteration of surface morphology, absence of microvilli and appearance of blebs. Caspase 3/7 assay results show that L. scandens dichloromethane extract produces the highest level of apoptosis against squamous carcinoma cells. For cell cycle analysis, all the L. scandens treated squamous carcinoma cells show high readings in the sub-G1 phase. Conclusion(s): This in vitro study has proved that L. scandens plant exhibit anti-proliferative effects against Squamous carcinoma cells, hence, it can be considered as a new promising potential anti-cancer therapy.

scholarly journals DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATR/p53/Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Jiechao Yang ◽  
Liang Zhou ◽  
Yanping Zhang ◽  
Juan Zheng ◽  
Jian Zhou ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Western Blot ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Molecular Mechanisms ◽  
Caspase 3 ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Poor Overall Survival

Cancer bioinformatics has been used to screen possible key cancer genes and pathways. Here, through bioinformatics analysis, we found that high expression of diaphanous related formin 1 (DIAPH1) was associated with poor overall survival in head and neck squamous cell carcinoma and laryngeal squamous cell carcinoma (LSCC). The effect of DIAPH1 in LSCC has not been previously investigated. Therefore, we evaluated the expression, function, and molecular mechanisms of DIAPH1 in LSCC. Immunohistochemistry and western blot analysis confirmed the significant upregulation of DIAPH1 in LSCC. We used DIAPH1 RNA interference to construct two DIAPH1-knockdown LSCC cell lines, AMC-HN-8 and FD-LSC-1, and validated the knockdown efficiency. Flow cytometry data showed that DIAPH1 inhibited apoptosis. Further, western blot analysis revealed that DIAPH1 knockdown increased the protein levels of ATR, p-p53, Bax, and cleaved caspase-3, -8, and -9. Thus, DIAPH1 is upregulated in LSCC and may act as an oncogene by inhibiting apoptosis through the ATR/p53/caspase-3 pathway in LSCC cells.

scholarly journals Arsenic trioxide enhances the cytotoxic effect of cisplatin in head and neck squamous cell carcinoma cell lines

2012 ◽  
Vol 3 (6) ◽  
pp. 1326-1330 ◽  
Author(s):  
ULANA KOTOWSKI ◽  
GREGOR HEIDUSCHKA ◽  
MARKUS BRUNNER ◽  
BOBAN M. EROVIC ◽  
HELGA MARTINEK ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Arsenic Trioxide ◽  
Cell Lines ◽  
Squamous Cell ◽  
Cytotoxic Effect ◽  
Carcinoma Cell ◽  
Squamous Cell Carcinoma Cell ◽  
Carcinoma Cell Lines

scholarly journals Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0180620 ◽  
Author(s):  
Pei-Feng Liu ◽  
Yu-Chang Hu ◽  
Bor-Hwang Kang ◽  
Yu-Kai Tseng ◽  
Pi-Chuang Wu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Caspase 3 ◽  
Tongue Squamous Cell Carcinoma ◽  
Oral Tongue ◽  
Expression Levels

scholarly journals Anti-mutagenic and synergistic cytotoxic effect of cisplatin and Honey Bee venom on 4T1 invasive mammary carcinoma cell line

2017 ◽  
Author(s):  
Faranak Shiassi Arani ◽  
Latifeh Karimzadeh ◽  
Seyed Mohammad Ghafoori ◽  
Mohammad Nabiuni
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Honey Bee ◽  
Mtt Assay ◽  
Sodium Azide ◽  
Mammary Carcinoma ◽  
Ames Test ◽  
Cytotoxic Effect ◽  
Mutagenic Activity ◽  
Bee Venom

ABSTRACTHoney Bee Venom has various biological activities such as inhibitory effect on several types of cancer. Cisplatin is an old and potent drug to treat the most of cancer. Our aims in this study were determination of the anti-mutagenic and cytotoxic effects of HBV on mammary carcinoma, lonely and in combination with cisplatin. In this study 4T1 cell line were cultured and incubated at 37 C in humidified CO2-incubator. The cell viabilities were examined by MTT assay. Also HBV was screened for its anti-mutagenic activity against sodium azide by Ames test. The result showed that 6μg/ml HBV, 20μg/ml cisplatin and 6μg/ml HBV with 10μg/ml cisplatin can induce an approximately 50% 4T1 cell death. 7mg/ml HBV with the inhibition of 62.76% sodium azide showed high potential in decreasing the mutagenic agents. MTT assay demonstrated that HBV and cisplatin can cause cell death in a dose-dependent manner. The cytotoxic effect of cisplatin is also promoted by HBV. Ames test results indicated that HBV can inhibit sodium azide as a mutagenic agent. Anti-mutagenic activity of HBV was increased significantly in presence of S9 mix. Hence, our findings reveal that HBV can enhance the cytotoxic effect of cisplatin drug and it has cancer preventing effects.

Sign in / Sign up

Export Citation Format

Share Document