Evaluation of the chemical composition of aquatic environments with leaf litter decomposition of Eucalyptus urophylla S.T. Blake (Myrtaceae) and their toxicity in Allium cepa L. (Amaryllidaceae)

Abstract Allelochemicals from eucalyptus released into the environment, mainly by leaves, can have a toxic effect on local biota, including in aquatic environments. Therefore, the present study evaluates the toxic activity of the water containing leaves of Eucalyptus urophylla S.T. Blake (Myrtaceae) in decomposition using Allium cepa L. (Amaryllidaceae) as a test organism. The toxicity and the cytotoxicity evaluation were performed using onion bulbs (A. cepa). The toxicity was assessed by inhibiting root growth. The cytotoxicity was evaluated by using a comparison of the mitotic index (MI) and the negative control. The extraction of eucalyptus metabolites from water of the decomposition tests and creek water was performed by solid-phase microextraction (SPME). The chemical characterization was done by gas chromatography coupled to mass spectrometry (GC-MS). There was inhibition of the root growth of A. cepa, indicating toxicity of the compounds released in the water during the decomposition. The cytotoxicity tests did not indicate a toxic effect. However, there were identified some mutations, cell death, and morphological changes in the roots. 26 compounds were identified on samples of water acquired from decomposition tests.Fenchone, 2-ethyl-1-hexanol, cis-dihydrocarvone, and trans-dihydrocarvone were identified in all samples. The results highlight the importance of studies and monitoring of aquatic environments near eucalyptus.

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Cytotoxic and Genotoxic Effects of Clopyralid Herbicide on Allium Cepa Roots

10.21203/rs.3.rs-223484/v1 ◽

2021 ◽

Author(s):

Eslem Amaç ◽

Recep Liman

Keyword(s):

Dna Damage ◽

Root Growth ◽

Growth Inhibition ◽

Allium Cepa ◽

Negative Control ◽

Root Growth Inhibition ◽

Genotoxic Effects ◽

Anaphase Bridges ◽

Auxin Herbicides ◽

Dose Dependent

Abstract Clopyralid is a one of the synthetic pyridine-carboxylate auxin herbicides and used to control perennial and annual broadleaf weeds in wheat, sugar beets and canola etc. In this study, dose dependent cytotoxicity and genotoxicity of clopyralid at different concentrations (25, 50, and 100 µg/mL) on the Allium cepa roots were evaluated at macroscopic (root growth) and microscopic levels (Mitotic index (MI), chromosome aberrations (CAs) in ana-telophase cells and DNA damage) using root growth inhibition, Allium ana-telophase and comet tests. The percentage root growth inhibition and concentration reducing root growth by 50% (EC50) of clopyralid in relation to the negative control were determined by using various concentrations of clopyralid (6.25–1000 µg/L). The 96 h EC50 of clopyralid was recorded as 50 µg/L. The gradual decrease in root growth and the MI reveals the cytotoxic effects of clopyralid. All the tested concentrations of clopyralid induced total CAs (polyploidy, stickiness, anaphase bridges, chromosome laggards, and disturbed ana-telophase) and DNA damage dose and time dependently. This study confirmed cytotoxic and genotoxic effects of clopyralid on non-target organism.

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Evaluation of an antimalarial herbal mixture and each extract for DNA and chromosomal mutations in Swiss albino mice and Allium cepa cells

Asian Journal of Medical and Biological Research ◽

10.3329/ajmbr.v7i3.56135 ◽

2021 ◽

Vol 7 (3) ◽

pp. 249-259

Author(s):

Akinboro Akeem ◽

Rufai Muhamed Akinlabi ◽

Ogunbameru Aanuoluwapo Dorcas ◽

Oladosu Bolaji Esther ◽

Taiwo Modinat Opeyemi

Keyword(s):

Root Growth ◽

Allium Cepa ◽

Sodium Azide ◽

Antioxidant Properties ◽

Water Extract ◽

Positive Control ◽

Negative Control ◽

Toxicological Evaluation ◽

Significant Difference ◽

The Individual

Toxicological evaluation of herbal medicines is necessary because of possible adverse effects that may be associated with their consumption. This study screened antimalarial herbal recipe (containing leaves of Azadirachta indica and stem-bark of Alstonia boonei) and its individual plant’s extract for DNA and chromosomes mutation potentials following the DNA fragmentation and Allium cepa assays. Superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) activity of the recipe and each extract was determined. The kinds of phytochemicals present in them were determined using the FTIR technique. Water extracts of A. indica, and A.boonei at all the tested doses caused significantly lower DNA fragmentations than those of the controls. However, at 25.0% and 50.0% recipe, there was no significant difference in the percentage fragmented DNA compared to the positive control (0.05% sodium azide). Cell division was significantly inhibited by the extracts and recipe, chromosomal aberrations were not dose dependently induced and were significantly lowered than that caused by sodium azide (positive control). The individual extracts and their recipe significantly inhibited Root growth. However, 12.5% recipe promoted root growth that was not significantly different from that of distilled water (negative control). SOD and CAT activities of each of the extracts and their recipe were dose dependent and significantly higher than those of the controls. Water extract of A. indica significantly suppressed generation of malondialdehyde compared to water extract of A. boonei and recipe as well as the control. The individual extracts and their recipe contained phenolic phytochemicals. The obtained results show that extract of A. indica, A. boonei and their recipe have good antioxidant properties with strong mitodepressive and root growth inhibitory effects except at 12.5% recipe. However, A. indica extract seems to have least cyto-muta-genotoxic effects than water extract of A. boonei and the recipe in mice and A. cepa cells. Asian J. Med. Biol. Res. 2021, 7 (3), 249-259

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Ability of PknA, a mycobacterial eukaryotic-type serine/threonine kinase, to transphosphorylate MurD, a ligase involved in the process of peptidoglycan biosynthesis

Biochemical Journal ◽

10.1042/bj20080234 ◽

2008 ◽

Vol 415 (1) ◽

pp. 27-33 ◽

Cited By ~ 45

Author(s):

Meghna Thakur ◽

Pradip K. Chakraborti

Keyword(s):

Protein Kinases ◽

Solid Phase ◽

Morphological Changes ◽

Binding Assays ◽

Serine Threonine Kinase ◽

Threonine Kinase ◽

Peptidoglycan Biosynthesis ◽

Vitro Binding ◽

Solid Phase Binding

Eukaryotic-type serine/threonine protein kinases in bacteria have been implicated in controlling a host of cellular activities. PknA is one of eleven such protein kinases from Mycobacterium tuberculosis which regulates morphological changes associated with cell division. In the present study we provide the evidence for the ability of PknA to transphosphorylate mMurD (mycobacterial UDP-N-acetylmuramoyl-L-alanine:D-glutamate-ligase), the enzyme involved in peptidoglycan biosynthesis. Its co-expression in Escherichia coli along with PknA resulted in phosphorylation of mMurD. Consistent with these observations, results of the solid-phase binding assays revealed a high-affinity in vitro binding between the two proteins. Furthermore, overexpression of m-murD in Mycobacterium smegmatis yielded a phosphorylated protein. The results of the present study therefore point towards the possibility of mMurD being a substrate of PknA.

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Protection against cisplatin-induced nephrotoxicity by a carbon monoxide-releasing molecule

AJP Renal Physiology ◽

10.1152/ajprenal.00363.2005 ◽

2006 ◽

Vol 290 (4) ◽

pp. F789-F794 ◽

Cited By ~ 89

Author(s):

Yasin Tayem ◽

Tony R. Johnson ◽

Brian E. Mann ◽

Colin J. Green ◽

Roberto Motterlini

Keyword(s):

Carbon Monoxide ◽

Caspase 3 ◽

Morphological Changes ◽

Antineoplastic Agent ◽

Negative Control ◽

Water Soluble ◽

Plasma Urea ◽

Therapeutic Benefits ◽

Corticomedullary Junction

Nephrotoxicity is one of the main side effects caused by cisplatin (CP), a widely used antineoplastic agent. Here, we examined the effect of a novel water-soluble carbon monoxide-releasing molecule (CORM-3) on CP-mediated cytotoxicity in renal epithelial cells and explored the potential therapeutic benefits of carbon monoxide in CP-induced nephrotoxicity in vivo. Exposure of LLC-PK1 cells to CP (50 μM) caused significant apoptosis as evidenced by caspase-3 activation and an increased number of floating cells. Treatment with CORM-3 (1–50 μM) resulted in a remarkable and concentration-dependent decrease in CP-induced caspase-3 activity and cell detachment. This effect involved activation of the cGMP pathway as 1H-oxadiazole [4, 3-a] quinoxaline-1-ore (ODQ), a guanylate cyclase inhibitor, completely abolished the protection elicited by CORM-3. Using a rat model of CP-induced renal failure, we found that treatment with CP (7.5 mg/kg) caused a significant elevation in plasma urea (6.6-fold) and creatinine (3.1-fold) levels, which was accompanied by severe morphological changes and marked apoptosis in tubules at the corticomedullary junction. A daily administration of CORM-3 (10 mg/kg ip), starting 1 day before CP treatment and continuing for 3 days thereafter, resulted in amelioration of renal function as shown by reduction of urea and creatinine levels to basal values, a decreased number of apoptotic tubular cells, and an improved histological profile. A negative control (iCORM-3) that is incapable of liberating CO failed to prevent renal dysfunction mediated by CP, indicating that CO is directly involved in renoprotection. Our data demonstrate that CORM-3 can be used as an effective therapeutic adjuvant in the treatment of CP-induced nephrotoxicity.

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Evaluation of Toxic, Cytotoxic, Mutagenic, and Antimutagenic Activities of Natural and Technical Cashew Nut Shell Liquids Using theAllium cepaandArtemia salinaBioassays

BioMed Research International ◽

10.1155/2015/626835 ◽

2015 ◽

Vol 2015 ◽

pp. 1-16 ◽

Cited By ~ 11

Author(s):

Aracelli de Sousa Leite ◽

Alisson Ferreira Dantas ◽

George Laylson da Silva Oliveira ◽

Antonio L. Gomes Júnior ◽

Sidney Gonçalo de Lima ◽

...

Keyword(s):

Allium Cepa ◽

Copper Sulfate ◽

Chromosomal Aberrations ◽

Artemia Salina ◽

Negative Control ◽

Anacardic Acid ◽

Toxic Effects ◽

Cashew Nut Shell Liquid ◽

Cashew Nut ◽

Cashew Nut Shell

The cashew nut releases a substance that is known as cashew nut shell liquid (CNSL). There are both natural (iCNSL) and technical (tCNSL) cashew nut shell liquids. This study used anArtemia salinabioassay to evaluate the toxic effects of iCNSL and tCNSL cashew nut shell liquids. It also evaluated the toxicity, cytotoxicity, and mutagenicity of CNSL and its effects on the damage induced by copper sulfate (CuSO4·5H2O) on the meristems’ root ofAllium cepa. Effects of the damage induced by CuSO4·5H2O were evaluated before (pre-), during (co-), and after (post-) treatments. The iCNSL contained 94.5% anacardic acid, and the tCNSL contained 91.3% cardanol. The liquids were toxic toA. salina. Toxicity, cytotoxicity, and mutagenicity were observed with iCNSL compared with the negative control. Similarly, iCNSL failed to inhibit the toxicity and cytotoxicity of CuSO4·5H2O. The tCNSL was not toxic, cytotoxic, or mutagenic in any of the concentrations. However, the lowest iCNSL concentrations and all of the tCNSL concentrations had preventive, antimutagenic, and reparative effects on micronuclei and on chromosomal aberrations in theA. cepa. Therefore, protective, modulating, and reparative effects may be observed in theA. cepa, depending on the concentration and type of CNSL used.

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Coal extraction causes sediment toxicity in aquatic environments in Santa Catarina, Brazil

Ambiente e Agua - An Interdisciplinary Journal of Applied Science ◽

10.4136/ambi-agua.2036 ◽

2017 ◽

Vol 12 (4) ◽

pp. 591 ◽

Cited By ~ 1

Author(s):

Lucimaira Amaral de Freitas ◽

Cassiano Lazarotto Rambo (in memoriam) ◽

Francini Franscescon ◽

Antônio Felipe Primon de Barros ◽

Guilherme Dos Santos De Lucca ◽

...

Keyword(s):

Heavy Metals ◽

Dissolved Oxygen ◽

Allium Cepa ◽

Chromosomal Aberrations ◽

Synergistic Effects ◽

Sediment Toxicity ◽

Negative Control ◽

Coal Extraction ◽

Santa Catarina ◽

Sediment Samples

This study evaluated water parameters in ponds affected by coal extraction. Allium cepa assay was used to measure genotoxicity/mutagenicity of the sediment. Samples were collected from four ponds in the southern state of Santa Catarina. Water temperature, pH, dissolved oxygen, conductivity and turbidity were measured. Sediments were analyzed for heavy metals. Elutriate samples were prepared at a ratio of 1:4 sediment:water. Allium cepa bulbs were placed in samples prepared from each pond, with ultrapure water used as negative control and methyl methane sulfonate as positive control. Root length, mitotic index, chromosomal aberrations, micronuclei, and nuclear abnormalities were measured. The pH of two ponds, as well as electrical conductivity and dissolved oxygen of all ponds were below the minimum limits set by Brazilian regulation. All heavy metals analyzed were found in all sediment samples, but only Cd concentration was above the legal limit set by Brazilian law. Allium cepa root growth for samples from Ponds 1, 2, and 4 was significantly lower than the negative control. Meristematic cells exposed to elutriate samples showed no significant changes in cell division. There was a significant increase in total chromosomal aberrations in all treated samples in comparison with the negative control. This study demonstrates that even low concentrations of heavy metals can damage exposed biota, possibly due to synergistic effects. We also found the A. cepa bioassay to be a simple and useful tool for genotoxicity/mutagenicity analyses, and recommend its use for environmental monitoring and management in areas influenced by mining activities.

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The effect of pH on copper toxicity to hydroponically grown maize.

Netherlands Journal of Agricultural Science ◽

10.18174/njas.v29i3.17008 ◽

1981 ◽

Vol 29 (3) ◽

pp. 217-238 ◽

Cited By ~ 6

Author(s):

T.M. Lexmond ◽

P.D.J. van der Vorm

Keyword(s):

Root Growth ◽

Toxic Effect ◽

Copper Toxicity ◽

Effect Of Ph ◽

Cu Toxicity ◽

Hydroponically Grown

The effect of pH on Cu toxicity in maize cv. Capella was studied in 3 sol. culture experiments of different design. Raising the pH intensified the toxic effect of Cu which reduced root growth and enhanced association of Cu2+ ions with physiologically essential sites in the roots when competition from protons was lowered. (Abstract retrieved from CAB Abstracts by CABI’s permission)

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Phyto-toxic Influence of an Atrazine-based Selective Herbicide – Arda-force® on Onions (Allium cepa L)

Chemical Science International Journal ◽

10.9734/csji/2020/v29i330169 ◽

2020 ◽

pp. 40-46

Author(s):

Doris Fovwe Ogeleka ◽

Esther Obasi

Keyword(s):

Root Growth ◽

Growth Inhibition ◽

Allium Cepa ◽

Environmental Protection Agency ◽

Toxic Effects ◽

Root Growth Inhibition ◽

Allium Cepa L ◽

Test Concentration ◽

Maximum Root ◽

Selective Herbicide

Introduction: The constant impact on the environment occasioned by pollution, indiscriminate application of agricultural chemicals, security challenges and crisis in the Niger Delta ecological area of Nigeria has caused severe damage to plants, soil organisms and humans. Aim and Methodology: In this research, onions (Allium cepa L) was exposed to varying concentrations of an atrazine-based selective herbicide Arda-force® to estimate the phyto-toxic effects on the plant species using the Organization for Economic Co-operation and Development, (OECD) protocol #208. Results: The mean effective concentration (EC50) using root growth inhibition produced indications of phyto-toxicity to the exposed species at a concentration of 0.55 ± 0.06 mg/L. Similarly, the maximum root growth inhibition efficiency relative to the control was 65% as recorded in the highest test concentration of 1.25 mg/L. Discussion: The study indicated that constant application / indiscriminate use of the herbicide Arda-force® could cause deleterious influence on these plant and vegetable species, daily consumed by humans as a rich source of anti-oxidants. Conclusion: This study concluded that atrazine-based herbicide Arda-force® used in this assessment resulted in phyto-toxic effects to Allium cepa L. At the exposed concentrations of the herbicide to non-target specie – Allium cepa L. that are integral parts of the ecosystems, the ‘‘harmless’’ status of atrazine acclaimed by the United State Environmental Protection Agency (USEPA) is still very much in doubt.

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