scholarly journals Listeria Species Occurrence and Associated Risk Factors and Antibiogram of Listeria Monocytogenes in Milk and Milk Products in Ambo, Holeta, and Bako Towns, Oromia Regional State, Ethiopia

2021 ◽  
Author(s):  
Bizunesh Mideksa Borena ◽  
Lemma Dilgasa ◽  
Endrias Zewdu Gebremedhin ◽  
Edilu Jorga Sarba ◽  
Lencho Megersa Marami ◽  
...  
Keyword(s):  
Risk Factors ◽  
Logistic Regression ◽  
Listeria Monocytogenes ◽  
Management System ◽  
Raw Milk ◽  
Farm Size ◽  
Isolation And Identification ◽  
Milk Products ◽  
Regional State ◽  
Associated Risk Factors

Abstract Background Listeriosis is a severe foodborne disease of significant burden to public health caused by consuming food contaminated with Listeria species. The current study was aimed to estimate the prevalence and associated risk factors of Listeria species and assess the antibiogram of Listeria monocytogenes (L. monocytogenes) isolated from milk and milk products from Holeta, Ambo, and Bako towns, Ethiopia. Methods A cross sectional study was employed and a systematic random sampling method was used to collect raw milk and milk products samples. A total of 482 samples (384 milk, 35 cottage cheeses, 30 bulk tank milk, and 33 curdle milk) were collected and isolation and identification of Listeria species were done using standard microbiological techniques. Antimicrobial susceptibility test for L. monocytogenes was performed using the Kirby-Bauer disk diffusion technique. Descriptive statistics was used to summarize the prevalence of Listeria while Chi-square test and logistic regression were used to determine the association between the prevalence of Listeria and the risk factors and the magnitude of association, respectively Results The isolation rate of Listeria species was 7.67% (37/482; 95% confidence interval (CI):5.46, 10.42). The overall prevalence of L. monocytogenes was 2.28%, (11/482; 95% CI: 1.14 - 4.04). The other species isolated were L. welshimeri 0.62% (3/482; 95% CI:0.13 - 1.81), L. seeligeri 1.04% (5/482; 95% CI:0.33 - 2.40), L. ivanovi 1.24%, (6/482;95% CI:0.45 - 2.68) and L. grayi 2.49% (12/482; 95% CI:5.46 - 10.42). Univariable logistic regression showed that study town, herd size, farm size, number of lactating cows, and management system were the factors significantly associated with for the isolation of Listeria species at farm level while intensive management system was independent predictor at cow level in the multivariable model (adjusted odds ratio = 3.38, P=0.046). L. monocytogenes isolates showed the highest resistance against oxacillin (100%), amoxicillin (90.91%), and vancomycine (81.82%). L. monocytogenes showed very high multi-drug resistance (MDR) [81.82%]. Conclusion The current study showed widespread of Listeria species MDR Listeria monocytogenes isolates in cow raw milk and milk products from Ambo, Holeta, and Bako towns, Oromia Regional State, Ethiopia.

scholarly journals Brucellosis in camel and human: Seroprevalence and associated risk factors in Amibara district of Afar region, Ethiopia

2021 ◽  
Author(s):  
Fekadu Gutema ◽  
Prof Kebede Amenu ◽  
Adugna Chalchisa ◽  
Prof Gezahegn Mamo
Keyword(s):  
Risk Factors ◽  
Close Contact ◽  
Mammalian Species ◽  
Raw Milk ◽  
Cross Sectional Study ◽  
Public Health Importance ◽  
Cross Sectional ◽  
North East ◽  
Afar Region ◽  
Associated Risk Factors

Abstract Background: Brucellosis is an important neglected zoonotic disease caused by infection with bacteria of the genus Brucella affecting different mammalian species including man. A cross-sectional study was conducted to estimate the seroprevalence of brucellosis in camels and human and its associated risk factors in Amibara district of Afar region, North east Ethiopia from October 2019 to May 2020Result: A total of 250 camel and 120 human sera were serially tested using the Rose Bengal Plate Test (RBPT), and Complement Fixation Test (CFT). The overall seroprevalence of camel brucellosis in the current study was 7.6% (95% CI: 4.9-11.56) using RBPT and 3.2% (95% CI: 1.63-6.2) by combined RBPT and CFT. In Human twelve (10%) of the collected sera were positive by RBPT among which only four of them (3.33%) were positive by CFT. The risk factors analysis indicated that, age, body condition, number of parity and abortion history were significantly associated with brucella seropositivity in camel(P≤0.05). In human, occupation and non-protective handling of dystocia cases showed apparent association with brucella seropositivity.Conclusion: The results of the present study indicated that, brucellosis is a common health problem in camel and human in Amibara district of Afar region. The public health importance of this disease is associated with raw milk consumption and close contact with the animals having history of recent abortion. Therefore, controlling the risk factors, establishing brucella diagnostic service in human clinics and hospitals, continuous social training with feedback assessments and overall implementing of One Health approach framework to attain optimal health for people and domestic animals in area are recommended to safeguard the health of society.

scholarly journals Seroprevalence of human Brucellosis and associated risk factors among high-risk occupations in Mbeya Region of Tanzania

2020 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Frederick D. Sagamiko ◽  
Ruth L. Mfune ◽  
Bernard M. Hang’ombe ◽  
Esron D. Karimuribo ◽  
Alfred M. Mwanza ◽  
...  
Keyword(s):  
Public Health ◽  
Risk Factors ◽  
Statistical Significance ◽  
Raw Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Brucellosis ◽  
P Value ◽  
Cross Sectional ◽  
Occupationally Exposed ◽  
Associated Risk Factors

Background: Brucellosis is a bacterial zoonotic disease of public health and economic importance. A cross-sectional study was conducted in Mbeya region between November 2015 and January 2016 to investigate the seroprevalence of human brucellosis and identify associated risk factors among individuals in risky occupations. Methods: A total of 425 humans from six occupational categories were serially tested for Brucella antibodies using the Rose Bengal Plate Test (RBPT) and competitive Enzyme Linked Immunosorbent Assay (c-ELISA), for screening and confirmation, respectively. A questionnaire survey was also administered to participants to collect epidemiological data. Results: The overall seroprevalence among the occupationally exposed individuals was 1.41% (95% CI: 00.64-3.12). Seroprevalence was higher among butcher men 5.6% (95% CI: 1.68-5.26), herds men 1.35% (95% CI: 0.18-9.02); and abattoir workers 1.1% (95% CI: 0.26-4.22) although there was no statistical significance. (P value = .18). Seroprevalence was also higher among men (1.8%) compared to females (0%) (P value = .19). and also, among those aged < 11 years (2.5%). Individuals who consumed raw milk had a higher seroprevalence (1.56%) compared to those who drunk boiled milk while seropositivity was 0.88% among those who assisted animals during parturition (P value = .49). Butcher men were at higher risk of exposure compared to other occupational categories. Our findings show the presence of brucellosis in occupationally exposed individuals in Mbeya region.Conclusion: There is need to sensitize the concerned professions in order to reduce the risk of acquiring Brucella infections from animals and animal products This also calls for public health awareness about the disease, and implementation of measures to prevent further spread of brucellosis within and outside the study area.

scholarly journals Risk Factors Associated with Contamination of Raw Milk by Listeria monocytogenes in Dairy Farms

1993 ◽  
Vol 76 (10) ◽  
pp. 2891-2898 ◽  
Author(s):  
M. Sanaa ◽  
B. Poutrel ◽  
J.L. Menard ◽  
F. Serieys
Keyword(s):  
Risk Factors ◽  
Listeria Monocytogenes ◽  
Dairy Farms ◽  
Raw Milk ◽  
Factors Associated

scholarly journals High HIV prevalence and associated risk factors among female sex workers in Rwanda

2017 ◽  
Vol 28 (11) ◽  
pp. 1082-1089 ◽  
Author(s):  
Mwumvaneza Mutagoma ◽  
Malamba S Samuel ◽  
Catherine Kayitesi ◽  
Antoine R Gasasira ◽  
Bassirou Chitou ◽  
...  
Keyword(s):  
Risk Factors ◽  
Logistic Regression ◽  
Hiv Infection ◽  
Sex Workers ◽  
Female Sex Workers ◽  
Hiv Prevalence ◽  
Consistent Condom ◽  
Prevention Strategies ◽  
Consistent Condom Use ◽  
Associated Risk Factors

Human immunodeficiency virus (HIV) prevalence is often high among female sex workers (FSWs) in sub-Saharan Africa. Understanding the dynamics of HIV infection in this key population is critical to developing appropriate prevention strategies. We aimed to describe the prevalence and associated risk factors among a sample of FSWs in Rwanda from a survey conducted in 2010. A cross-sectional biological and behavioral survey was conducted among FSWs in Rwanda. Time–location sampling was used for participant recruitment from 4 to 18 February 2010. HIV testing was done using HIV rapid diagnostic tests (RDT) as per Rwandan national guidelines at the time of the survey. Elisa tests were simultaneously done on all samples tested HIV-positive on RDT. Proportions were used for sample description; multivariable logistic regression model was performed to analyze factors associated with HIV infection. Of 1338 women included in the study, 1112 consented to HIV testing, and the overall HIV prevalence was 51.0%. Sixty percent had been engaged in sex work for less than five years and 80% were street based. In multivariable logistic regression, HIV prevalence was higher in FSWs 25 years or older (adjusted odds ratio [aOR] = 1.83, 95% [confidence interval (CI): 1.42–2.37]), FSWs with consistent condom use in the last 30 days (aOR = 1.39, [95% CI: 1.05–1.82]), and FSWs experiencing at least one STI symptom in the last 12 months (aOR = 1.74 [95% CI: 1.34–2.26]). There was an inverse relationship between HIV prevalence and comprehensive HIV knowledge (aOR = 0.65, [95% CI: 0.48–0.88]). HIV prevalence was high among a sample of FSWs in Rwanda, and successful prevention strategies should focus on HIV education, treatment of sexually transmitted infections, and proper and consistent condom use using an outreach approach.

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