scholarly journals STUDY ON OCCURRENCE OF LISTERIA MONOCYTOGENES IN SOIL OF PUNJAB PROVINCE AND ITS ASSOCIATED RISK FACTORS

2021 ◽  
Author(s):  
Bizunesh Mideksa Borena ◽  
Lemma Dilgasa ◽  
Endrias Zewdu Gebremedhin ◽  
Edilu Jorga Sarba ◽  
Lencho Megersa Marami ◽  
...  

Abstract Background Listeriosis is a severe foodborne disease of significant burden to public health caused by consuming food contaminated with Listeria species. The current study was aimed to estimate the prevalence and associated risk factors of Listeria species and assess the antibiogram of Listeria monocytogenes (L. monocytogenes) isolated from milk and milk products from Holeta, Ambo, and Bako towns, Ethiopia. Methods A cross sectional study was employed and a systematic random sampling method was used to collect raw milk and milk products samples. A total of 482 samples (384 milk, 35 cottage cheeses, 30 bulk tank milk, and 33 curdle milk) were collected and isolation and identification of Listeria species were done using standard microbiological techniques. Antimicrobial susceptibility test for L. monocytogenes was performed using the Kirby-Bauer disk diffusion technique. Descriptive statistics was used to summarize the prevalence of Listeria while Chi-square test and logistic regression were used to determine the association between the prevalence of Listeria and the risk factors and the magnitude of association, respectively Results The isolation rate of Listeria species was 7.67% (37/482; 95% confidence interval (CI):5.46, 10.42). The overall prevalence of L. monocytogenes was 2.28%, (11/482; 95% CI: 1.14 - 4.04). The other species isolated were L. welshimeri 0.62% (3/482; 95% CI:0.13 - 1.81), L. seeligeri 1.04% (5/482; 95% CI:0.33 - 2.40), L. ivanovi 1.24%, (6/482;95% CI:0.45 - 2.68) and L. grayi 2.49% (12/482; 95% CI:5.46 - 10.42). Univariable logistic regression showed that study town, herd size, farm size, number of lactating cows, and management system were the factors significantly associated with for the isolation of Listeria species at farm level while intensive management system was independent predictor at cow level in the multivariable model (adjusted odds ratio = 3.38, P=0.046). L. monocytogenes isolates showed the highest resistance against oxacillin (100%), amoxicillin (90.91%), and vancomycine (81.82%). L. monocytogenes showed very high multi-drug resistance (MDR) [81.82%]. Conclusion The current study showed widespread of Listeria species MDR Listeria monocytogenes isolates in cow raw milk and milk products from Ambo, Holeta, and Bako towns, Oromia Regional State, Ethiopia.


2016 ◽  
Author(s):  
Swati Waghdhare ◽  
Neelam Kaushal ◽  
Rajinder K Jalali ◽  
Divya Vohora ◽  
Sujeet Jha

Author(s):  
Md Sheikh ◽  
Manahel Alotaibi ◽  
Nouf Almutairi ◽  
Eid Aljohani ◽  
Omar Alruwaili ◽  
...  

2021 ◽  
Author(s):  
Ekaterina Mosolova ◽  
Dmitry Sosin ◽  
Sergey Mosolov

During the COVID-19 pandemic, healthcare workers (HCWs) have been subject to increased workload while also exposed to many psychosocial stressors. In a systematic review we analyze the impact that the pandemic has had on HCWs mental state and associated risk factors. Most studies reported high levels of depression and anxiety among HCWs worldwide, however, due to a wide range of assessment tools, cut-off scores, and number of frontline participants in the studies, results were difficult to compare. Our study is based on two online surveys of 2195 HCWs from different regions of Russia during spring and autumn epidemic outbreaks revealed the rates of anxiety, stress, depression, emotional exhaustion and depersonalization and perceived stress as 32.3%, 31.1%, 45.5%, 74.2%, 37.7% ,67.8%, respectively. Moreover, 2.4% of HCWs reported suicidal thoughts. The most common risk factors include: female gender, nurse as an occupation, younger age, working for over 6 months, chronic diseases, smoking, high working demands, lack of personal protective equipment, low salary, lack of social support, isolation from families, the fear of relatives getting infected. These results demonstrate the need for urgent supportive programs for HCWs fighting COVID-19 that fall into higher risk factors groups.


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