scholarly journals In Vitro, Evaluation of Anticancer and Anti-dermatophytes Activities of Some Egyptian Medicinal Plants 

2020 ◽  
Author(s):  
Esraa Ahmed Mohamed El-Bondkly ◽  
Mervat Morsy El-Gendy ◽  
Aya Ahmed Mohamed El-Bondkly ◽  
Fareed Shawky El-Shenawy ◽  
Alaa Ahmed Mohamed El-Bondkly
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Methanolic Extract ◽  
Acetone Extract ◽  
Microsporum Canis ◽  
Microsporum Gypseum ◽  
Hela Cell Lines ◽  
Trichophyton Soudanense ◽  
Hct 116 ◽  
Mcf 7

Abstract In this investigation three ancient Egyptian medical plants; Plantago albicans L., Thymelaea hirsuta (L.) Endl. and Urginea maritima (L.) were chosen to explore their biochemical properties, anticancer and antimycotic activities against clinical dermatophytes. Growing of Trichophyton soudanense, Trichophyton erinacei, Microsporum audouinii, Microsporum gypseum, Microsporum gallinae, Microsporum ferrogenium, Microsporum cookie, Microsporum racemosum, Microsporum persicolor and Microsporum canis were totally inhibited by the 100 µg/mL of P. albicans methanolic extract. Whereas the growth of T. soudanense, T. erinacei, Trichophyton rubrum, Trichophyton tonsurance and Trichophyton mentagrophytes and Epidermophyton floccosum were 100% inhibited by the methanolic extract of T. hirsiuta at 100 µg/mL concentration, however its acetone extract was more active against Microsporum species. The highest fungi toxicity against all dermatophytic fungi was detected in both methanol and acetone extract of U. maritima at a concentration ranged between 50 to 100 µg/mL. Methanolic extract of P. albicans inhibited the viability of HCT-116, HepG-2, MCF-7 and HeLa cell lines by (41%, 40%, 80% and 81%); (30%, 20%, 66% and 70%); (19%, 0%, 49% and 57%); (5%, 0%, 35% and 45%) and (0%, 0%, 21% and 40%) at 25, 50, 100, 200 and 300 µg/mL, respectively. Moreover, the cells death after treatment with the same concentrations of T. hirsuta methanol extract were (10%, 20%, 23% and 60%); (16%, 40%, 58% and 80%); (40%, 50%, 80% and 100%); (51%, 65%, 100% and 100%) and (49%, 82%, 100% and 100%) in HCT-116, HepG-2, MCF-7 and HeLa cells, respectively. Viability of HCT-116, MCF-7 and HepG-2 cell lines was totally suppressed with 100 µg/mL whereas the HeLa cell growth was reduced to 10% and totally killed at 100 and 200 µg/mL, respectively of U. maritima methanolic extract. All data approve the significance of Egyptian ethnomedical plants as potent source of diverse bioactive pharmaceutical metabolites.

scholarly journals Structures, Chemical Conversions, and Cytotoxicity of Tricholopardins C and D, Two Tricholoma Triterpenoids from the Wild Mushroom Tricholoma pardinum

2021 ◽  
Author(s):  
Chen Shi ◽  
Yue-Ling Peng ◽  
Juan He ◽  
Zheng-Hui Li ◽  
Ji-Kai Liu ◽  
...  
Keyword(s):  
Hela Cell ◽  
Single Crystal ◽  
Cell Lines ◽  
Cell Apoptosis ◽  
Spectroscopic Methods ◽  
Wild Mushroom ◽  
X Ray Diffraction ◽  
X Ray ◽  
Hela Cell Lines ◽  
Mcf 7

AbstractTwo undescribed Tricholoma triterpenoids, namely tricholopardins C (1) and D (2), were isolated from the wild mushroom Tricholoma pardinum. Their structures with absolute configurations were elucidated by spectroscopic methods, as well as the single crystal X-ray diffraction. Compounds 1 and 2 were further obtained by chemical conversions from the known analogues. Compound 1 showed significant cytotoxicity to MCF-7 and Hela cell lines with IC50 values of 4.7 μM and 9.7 μM, respectively. Its mechanism of inducing MCF-7 cell apoptosis was studied briefly. Graphical Abstract

Radiation dose rate affects the radiosensitization of MCF-7 and HeLa cell lines to X-rays induced by dextran-coated iron oxide nanoparticles

2017 ◽  
Vol 93 (8) ◽  
pp. 757-763 ◽  
Author(s):  
Karim Khoshgard ◽  
Parvaneh Kiani ◽  
Abbas Haghparast ◽  
Leila Hosseinzadeh ◽  
Mohammad Taghi Eivazi
Keyword(s):  
Iron Oxide ◽  
Radiation Dose ◽  
Hela Cell ◽  
Dose Rate ◽  
Cell Lines ◽  
Oxide Nanoparticles ◽  
X Rays ◽  
Radiation Dose Rate ◽  
Hela Cell Lines ◽  
Mcf 7

scholarly journals Dipterostilbenosides A and B, Oligostilbene Glycosides from Dipterocarpus tuberculatus

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Serm Surapinit ◽  
Jonkolnee Jong-aramruang ◽  
Pongpun Siripong ◽  
Suttira Khumkratok ◽  
Santi Tip-pyang
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Spectroscopic Data ◽  
Methanolic Extract ◽  
Chemical Investigation ◽  
Hela Cell Lines

From the chemical investigation of the methanolic extract of the roots of the Thai dipterocarp, Dipterocarpus tuberculatus, two new oligostilbene glycosides, dipterostilbenes A (1) and B (2), were isolated together with four known stilbenes. Their structures and relative configurations were determined on the basis of spectroscopic data. From an evaluation of cytotoxic activity against KB and HeLa cell lines, α-viniferin (5) and (-)-hopeaphenol (6) showed potent activity, but less than that of doxorubicin.

scholarly journals Synthesis, Anticancer Evaluation and Structure-Activity Analysis of Novel (E)- 5-(2-Arylvinyl)-1,3,4-oxadiazol-2-yl)benzenesulfonamides

2020 ◽  
Vol 21 (6) ◽  
pp. 2235
Author(s):  
Krzysztof Szafrański ◽  
Jarosław Sławiński ◽  
Łukasz Tomorowicz ◽  
Anna Kawiak
Keyword(s):  
Anticancer Activity ◽  
Cell Lines ◽  
Human Tumor ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Structure Activity ◽  
Hela Cell Lines ◽  
Hct 116 ◽  
Mcf 7

To learn more about the structure–activity relationships of (E)-3-(5-styryl-1,3,4-oxadiazol-2-yl)benzenesulfonamide derivatives, which in our previous research displayed promising in vitro anticancer activity, we have synthesized a group of novel (E)-5-[(5-(2-arylvinyl)-1,3,4-oxadiazol-2-yl)]-4-chloro-2-R1-benzenesulfonamides 7–36 as well as (E)-4-[5-styryl1,3,4-oxadiazol-2-yl]benzenesulfonamides 47–50 and (E)-2-(2,4-dichlorophenyl)-5-(2-arylvinyl)-1,3,4-oxadiazols 51–55. All target derivatives were evaluated for their anticancer activity on HeLa, HCT-116, and MCF-7 human tumor cell lines. The obtained results were analyzed in order to explain the influence of a structure of the 2-aryl-vinyl substituent and benzenesulfonamide scaffold on the anti-tumor activity. Compound 31, bearing 5-nitrothiophene moiety, exhibited the most potent anticancer activity against the HCT-116, MCF-7, and HeLa cell lines, with IC50 values of 0.5, 4, and 4.5 µM, respectively. Analysis of structure-activity relationship showed significant differences in activity depending on the substituent in position 3 of the benzenesulfonamide ring and indicated as the optimal meta position of the sulfonamide moiety relative to the oxadizole ring. In the next stage, chemometric analysis was performed basing on a set of computed molecular descriptors. Hierarchical cluster analysis was used to examine the internal structure of the obtained data and the quantitative structure–activity relationship (QSAR) analysis with multiple linear regression (MLR) method allowed for finding statistically significant models for predicting activity towards all three cancer cell lines.

Anticancer properties of methanolic extract of Piper crocatum leaf using BST and cytotoxicity on HeLa cell lines

2020 ◽  
Vol 23 (03) ◽  
pp. 01-09
Author(s):  
Afrian Rosyadi ◽  
Renny Nurul Faizah ◽  
Nuri Nuri ◽  
Endah Puspitasari
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Methanolic Extract ◽  
Anticancer Properties ◽  
Hela Cell Lines

Unpredicted formation of copper(ii) complexes containing 2-thiophen-2-yl-1-thiophen-2-ylmethyl-1H-benzoimidazole and their most promising in vitro cytotoxicity in MCF-7 and HeLa cell lines over cisplatin

2017 ◽  
Vol 41 (17) ◽  
pp. 8885-8898 ◽  
Author(s):  
P. Naveen ◽  
Ruchi Jain ◽  
P. Kalaivani ◽  
R. Shankar ◽  
F. Dallemer ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Sodium Azide ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Lines ◽  
Mcf 7

An in situ reaction of CuCl2·2H2O, o-phenylenediamine, thiophene-2-carbaldehyde and sodium azide in methanol afforded complex 1a.

scholarly journals Evaluation of cytotoxic and anticancer effect of Orobanche crenata methanolic extract on cancer cell lines

Tumor Biology ◽  
2020 ◽  
Vol 42 (5) ◽  
pp. 101042832091868 ◽  
Author(s):  
Marwa GA Hegazy ◽  
Amal M Imam ◽  
Bassem E Abdelghany
Keyword(s):  
Antioxidant Activity ◽  
Lactate Dehydrogenase ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Caspase 3 ◽  
Methanolic Extract ◽  
Orobanche Crenata ◽  
Hct 116 ◽  
Dose Dependent ◽  
Mcf 7

We aimed to assess the antitumor activity of Orobanche crenata methanolic extract and evaluate its cytotoxic effect on different cancer cell lines to develop an effective natural anticancer drug. Components of O. crenata methanolic extract were analyzed using gas chromatography–mass spectrometry. The extract’s antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power procedures and cytotoxicity of the extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase assays. Caspase-3 activity was also estimated. O. crenata methanolic extract shows powerful antioxidant activity. The extract inhibited the propagation of human hepatocellular carcinoma (HepG2), human prostate cancer (PC3), human breast adenocarcinoma (MCF-7), and human colon carcinoma (HCT-116) in a dose-dependent manner. O. crenata–treated cells displayed obvious morphological structures distinctive of apoptosis. MTT assay exposed that the extract presented prevention of cell persistence in a dose-dependent means and revealed extremely cytotoxic activity against HepG2, PC3, MCF-7, and HCT-116 with 50% inhibitory concentration values 30.3, 111, 89.6, and 28.6 µg/mL, respectively, after 24 h of incubation. In addition, treatment of HCT-116 with various concentrations of the extract caused the release of lactate dehydrogenase and induction of caspase-3 activity in a dose-dependent way. In conclusion, our findings suggested that the O. crenata extract possesses potent antioxidant, cytotoxic activity, and anticancer properties which are possibly due to the principal bioactive phytochemical composites existing in this plant. These results can be used to develop new drugs for cancer treatment.

Anticancer effect of A-ring or/and C-ring modified oleanolic acid derivatives on KB, MCF-7 and HeLa cell lines

2012 ◽  
Vol 10 (11) ◽  
pp. 2201 ◽  
Author(s):  
Barbara Bednarczyk–Cwynar ◽  
Lucjusz Zaprutko ◽  
Piotr Ruszkowski ◽  
Bogusław Hładoń
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Oleanolic Acid ◽  
Anticancer Effect ◽  
Hela Cell Lines ◽  
Mcf 7
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