scholarly journals 230 MOLECULAR MARKERS FOR EARLY DETERMINATION OF SEX OF PISTACHIO SEEDLINGS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 462d-462
Author(s):  
J.I. Hormaza ◽  
L. Dollo ◽  
V.S. Polito
Keyword(s):  
Molecular Markers ◽  
Random Amplified Polymorphic Dna ◽  
Dna Amplification ◽  
Sex Expression ◽  
Pistacia Vera ◽  
Dioecious Species ◽  
Reliable Marker ◽  
The Relationship ◽  
Made In

The Random Amplified Polymorphic DNA (RAPD) technique was used to develop molecular markers linked to sex expression in Pistacia vera, a dioecious species. Progenies from two female parents (`Lassen' and `Kerman') pollinated by a common male parent (`Peters') were studied. Two bulks of DNA were made in each cross, one from males and one from females. DNA was extracted from each bulked sample as well as from each of the contributing individuals and from 14 additional P. vera cultivars. Twelve hundred decamer oligonucleotide primers were used to perform DNA amplification on the bulk DNA. This analysis led to the identification of one primer (OPO08) that produces a 945 bp. amplification band present only in females and absent in males. The relationship between band presence and female sex expression was conserved in every individual obtained from the two crosses and in 14 cultivars unrelated to the crosses. This band, which we propose is tightly linked to the gene(s) controlling sex determination, provides a reliable marker for sex of pistachio seedlings and should be a useful tool in pistachio breeding.

Determination of Underwater Plate Dynamics Using Laser Beams

1974 ◽  
Vol 96 (3) ◽  
pp. 722-728
Author(s):  
Rudolph E. Croteau ◽  
Herman E. Sheets
Keyword(s):  
Near Field ◽  
Surface Displacement ◽  
Coupled System ◽  
Green Laser ◽  
Testing Facility ◽  
Acoustic Testing ◽  
Propeller Blades ◽  
The Relationship ◽  
Made In

Underwater plate vibration and its associated noise are of interest for the analysis of ship structures, propeller blades, and other areas of underwater acoustics. In order to analyze the relationship between a plate vibrating underwater and the acoustic pressure in the near-field, optical interferometric holography, using a blue-green laser beam, was used to determine surface displacement for the vibrating plate, which was excited through a fluid-coupled system. Acoustic measurements of the same source were made in a water tower concurrently with the holography and later at a precision acoustic testing facility. This method permits prediction of underwater plate modal frequencies and shapes with high accuracy.

scholarly journals Characterization of Mexican Isolates of Colletotrichum lindemuthianum by Using Differential Cultivars and Molecular Markers

1998 ◽  
Vol 88 (4) ◽  
pp. 292-299 ◽  
Author(s):  
Mario González ◽  
Raul Rodríguez ◽  
Maria Elena Zavala ◽  
Juan L. Jacobo ◽  
Fernando Hernández ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Location ◽  
Genetic Distances ◽  
Colletotrichum Lindemuthianum ◽  
Aflp Data ◽  
Bootstrap Analysis ◽  
Differential Cultivars ◽  
Bean Anthracnose

Differential cultivars and molecular markers were used to analyze 59 isolates of the bean anthracnose pathogen, Colletotrichum lindemuthianum, from different regions of Mexico. Ten distinct races were determined, three of which had not been reported previously in Mexico. Isolates were found to infect only a narrow range of the differential cultivars used and were restricted to cultivars of Middle American origin. A comparison of random amplified polymorphic DNA and amplified fragment length polymorphism (AFLP) analyses was carried out on a subset of the fungal isolates. Determination of genetic distances based on AFLP data and production of a dendrogram demonstrated two levels of association: i) isolates classified into two major groups according to the type of cultivar or system of cultivation from which they originated, and ii) isolates could be classified into smaller subgroups generally associated with the geographic location from which they were obtained. Bootstrap analysis and determination of confidence intervals showed these geographic groupings to be extremely robust.

Determination of -carotene content in Musa AA pulp (Kluai Khai) at different ripening stage and harvest period in Thailand

2020 ◽  
pp. 443
Author(s):  
Kongaphisith Tongpoolsomjit ◽  
François Grandmottet ◽  
Ratri Boonruangrod ◽  
Akharapong Krueajan, Jarupa Viyoch
Keyword(s):  
Flow Cytometry ◽  
Molecular Markers ◽  
Climate Variability ◽  
Soluble Solids ◽  
Ripening Stage ◽  
Fresh Weight ◽  
Carotene Content ◽  
Total Soluble Solids ◽  
The Relationship

Musa AA ‘Kluai Khai’ banana, is an important economic crop in Thailand. It was evaluated according to its morphology, total soluble solids (TSS), and pulp b-carotene content over different months according to its ripening state. The climate variability of Thailand in 2019 resulted in smaller bananas in May than in other months (March and July). TSS was found to be a directly proportional correlation to the ripening stage. The genotype and ploidy of Musa AA ‘Kluai Khai’ were confirmed using molecular markers and flow cytometry. The yellowish pigment of carotenoid was extracted from banana pulp using THF:MeOH (1:1, v/v). The utilization of HPLC displayed b-carotene content in Musa AA ‘Kluai Khai’ pulp as high as 5222.6 ± 83.8 µg / 100 g of fresh weight (gfw) in the 4th state of banana development in March, which was higher than those in July (4072.8 µg/ 100 gfw) and May (3121.8 µg/ 100 gfw) for the same ripening state. The relationship between b-carotene content and the ripening state was calculated to be a logarithmic correlation.

scholarly journals Genetic variations of strawberry cultivars of Fragaria x ananassa and Fragaria vesca based on RAPD

2019 ◽  
Vol 20 (3) ◽  
pp. 770-775
Author(s):  
GANIES RIZA ARISTYA ◽  
RINA SRI KASIAMDARI ◽  
RACHMI SETYONINGRUM ◽  
BENING LARASATI
Keyword(s):  
Genetic Variation ◽  
Molecular Markers ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Index ◽  
Dna Amplification ◽  
Genetic Variations ◽  
Fragaria X Ananassa ◽  
Fragaria Vesca ◽  
Market Demand ◽  
Strawberry Cultivars

Abstract. Aristya GR, Kasiamdari RS, Setyoningrum R, Larasati B. 2019. Genetic variations of strawberry cultivars of Fragaria x ananassa and Fragaria vesca based on RAPD. Biodiversitas 20: 770-775. In Indonesia, the increasing market demand for strawberries (Fragaria spp.) is not comparable to increased strawberry productivity. One of the efforts made to increase strawberry productivity with superior quality is plant breeding. The purpose of this research was to determine the genetic variation, lineage, and similarity index in some strawberry cultivars using molecular markers of Random Amplified Polymorphic DNA (RAPD). Eleven strawberry cultivar samples were taken from Indonesian Citrus and Subtropical Fruits Research Institute (Balitjestro), Batu City, East Java, Indonesia and Strawberry Agritourism in Banyuroto Village, Magelang District, Central Java, Indonesia. DNA isolation using modified CTAB buffer method. DNA amplification using PCR-RAPD method with 5 primers, namely UBC-516, UBC-594, OPA 10, OPA 16, and OPG 11. Strawberry lineage dendrogram construction was analyzed with clustering of Unweight Pair-Group Using Arithmetic Average (UPGMA) software Multi-Variate Statistical Average (MVSP). The research results showed that the 5 RAPD primers used in 11 strawberry cultivars produced 30 polymorphic DNA bands and 20 monomorphic DNA bands so it can be concluded that the genetic variation among 11 strawberry cultivars can be detected using RAPD molecular markers. The lineage of 11 strawberry cultivars that have the highest similarity index is found in Earlibrite and Rosalinda II cultivars of 98.85%.

scholarly journals GENETIC CHARACTERIZATION OF GALOBA DURIAN (AMONUM SPP.) IN AMBON ISLAND BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

2018 ◽  
Vol 3 (1) ◽  
pp. 27-33
Author(s):  
Fuadiska Salamena ◽  
Cecilia Anna Seumahu
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Characterization ◽  
Kidney Diseases ◽  
Random Amplified Polymorphic Dna ◽  
Quantitative Result ◽  
Dna Amplification ◽  
Polymorphic Band ◽  
Qualitative And Quantitative

Galoba durian is one of the endemic floras from Maluku. Galoba durian is a species belonging to the Amomum genera Zingiberaceae family. It is also used as a medicinal plant for waist and kidney diseases. Based on the color of the fruit, galoba durian is divided into two nmely red galoba durian and green galoba durian. Distribution of this plant in Ambon can found in a few places such as highland and coastal area. Different locations influence phenotypic of plants, but may not show different genetic characteristic. Genetic diversity can detected by molecular markers. Genetic characterization from galoba durian using RAPD markers has not been done before. This study aimed to analyze genetic diversity from galoba durian using molecular markers RAPD. Samples of plants are used red galoba durian from Hatu and green galoba durian from Hatalae. The result of the first study, characterization of the morphology of the galoba durian, showed that both galoba have almost similar characteristics. Further DNA was tested by qualitative and quantitative. Result shows good qualitative and quantitative of DNA genomic. The second study was amplification by PCR-RAPD. DNA amplifications were performed using 3 primers out of 9 screened random primers. The primers selection was based on hight polymorphism. DNA amplification has 36 bands which were 100% polymorphic. The size of each bands from visualization of agarose was determined by linear regression. Number of band amplified was range from 120 to 1612 bp. Polymorphic band of RAPD showed the highest  genetic diversity. It can be concluded that the two plants of galoba durian are different species

scholarly journals OPTIMASI SUHU ANNEALING UNTUK EMPAT PRIMER RAPD PADA KACANG HIJAU (Vigna radiata L.)

2019 ◽  
Vol 34 (1) ◽  
pp. 41-46
Author(s):  
Herman Herman ◽  
Martupa Nainggolan ◽  
Dewi Indriyani Roslim
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Annealing Temperature ◽  
Random Amplified Polymorphic Dna ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Temperature Optimization ◽  
Total Dna

Determination of annealing temperature of the primer is the first step for genetic diversity analysis using molecular markers such as RAPD (Random Amplified Polymorphic DNA). This study aims to determine annealing temperature (Ta) of RAPD primers on Kampar Mungbean. Methods included total DNA extraction, electrophoresis, and annealing temperature optimization of four RAPD markers namely OPD-20, OPI-06, OPI-13, dan OPX-13. Optimization was conducted by reducing the Tm value (Time melting) of each primer with 3 (Tm-3) and 5 (Tm-5). The results showed that the optimization using OPD-20 and OPX-13 produced bands at Tm-3 and Tm-5. Meanwhile, optimization using OPI-06 and OPI-13 resulted in bands at Tm-3. The next step was to choose the exact Ta based on the clear and bright band. In conclusion, exact Ta for OPD-20, OPI-06, OPI-13, and OPX-13 were 36,1°C, 38,1°C, 35,4°C, and 32,5°C respectively.

scholarly journals El uso de los RAPDs como marcadores moleculares en plantas

2017 ◽  
pp. 85
Author(s):  
Adriana Otero-Arnaiz ◽  
Marlene De la Cruz ◽  
Ken Oyama
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Structure ◽  
Conservation Biology ◽  
Population Genetic ◽  
Mapping Population ◽  
Random Amplified Polymorphic Dna ◽  
Interspecific Variation ◽  
Genetic Identification

In this paper a review of RAPDs (Random Amplified Polymorphic DNA) as molecular markers is presented. This molecular marker has been used to genetic mapping, population genetic structure, genetic identification, intra and interspecific variation, reconstructing phylogenies and conservation biology. In general, RAPDs have been useful to estimate genetic diversity, genetic identification of taxa, clones, varieties and hybrids. However, their use in Systematic has been questioned due to the difficulties in the determination of identity, homologies and independence of characters. Methods of analyses used to estimate genetic diversity are briefly described. Finally, some recent advances and new technics based on PCR are presented.

scholarly journals RAPD Marker for Sex Determination of Dioecious Plants

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 878F-878 ◽  
Author(s):  
Deepak K. Khandka ◽  
Ali Nejidat ◽  
Moshe Tal ◽  
Avi Golan-Goldhirsh
Keyword(s):  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Sex Differentiation ◽  
Rapd Marker ◽  
Specific Marker ◽  
Dioecious Species ◽  
Important Species ◽  
Male Specific

Several horticulturally important species are dioecious (e.g., pistachio, date palm, poplar, and others). It would be advantageous if the gender of a seedling could be determined at the vegetative stage. In this report, we present results of our search for molecular markers for sex differentiation in dioecious species. The method used was bulked segregant analysis of random amplified polymorphic DNA (RAPD) for sex. A male-specific marker fragment OPB01-1470 was obtained in Mercurialis annua. The sex linkage and characterization of this marker will be discussed.

scholarly journals HYPERGLYCEMIA AND INCIDENCE OF FRAILTY IN OLDER MEXICAN ADULTS LIVING IN RURAL AREAS

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S680-S680
Author(s):  
Betty Manrique Espinoza ◽  
Ana Rivera Almaraz ◽  
Aaron Salinas Rodriguez
Keyword(s):  
Older Adults ◽  
Rural Areas ◽  
Glycosylated Hemoglobin ◽  
High Prevalence ◽  
Mexican Adults ◽  
The Relationship ◽  
Ordinal Logistic Regression Model ◽  
Made In ◽  
Rural Older Adults

Abstract Hyperglycemia is the main characteristic of diabetes and is the result of an absolute or partial deficit in the production or action of insulin. Recent evidence suggests that hyperglycemia increases the risk of frailty. This issue is of great importance for the Mexican population given the high prevalence of diabetes, particularly in older adults. Our objective was to analyze the association between hyperglycemia and the incidence of frailty in a cohort of rural older adults in Mexico. Prospective cohort study with 600 rural older adults, with measurements made in 2009, 2013 and 2018. Fragility was defined using the proposal of Fried and colleagues. The determination of glycosylated hemoglobin was performed through the A1CNow® device, with capillary blood; hyperglycemia was defined considering the recommendation of the American Diabetes Association; where values greater than 6.5% (140 mg / dL) of glycosylated hemoglobin were considered hyperglycemia. We used an ordinal logistic regression model to analyze the relationship between hyperglycemia and incidence of frailty. In the baseline measurement (2009), 8.6% of older adults presented frailty. The incidence of frailty was 6.9%. After adjusting for health and sociodemographic characteristics, hyperglycemia was significantly associated with the incidence of frailty (RR = 2.24 P = 0.018). These findings allow us to determine that hyperglycemia is a prognostic factor of the incidence of frailty. Because frailty is preventable, future interventions for the prevention of frailty should consider the presence of hyperglycemia.

The quantitative comparison of experimental and simulated diffraction contrast images

1995 ◽  
Vol 53 ◽  
pp. 102-103
Author(s):  
Stuart McKernan
Keyword(s):  
Image Processing ◽  
Personal Computer ◽  
Close Agreement ◽  
Quantitative Comparison ◽  
Image Simulation ◽  
Computing Power ◽  
Diffraction Contrast ◽  
Simulated Images ◽  
Made In

For many years the concept of quantitative diffraction contrast experiments might have consisted of the determination of dislocation Burgers vectors using a g.b = 0 criterion from several different 2-beam images. Since the advent of the personal computer revolution, the available computing power for performing image-processing and image-simulation calculations is enormous and ubiquitous. Several programs now exist to perform simulations of diffraction contrast images using various approximations. The most common approximations are the use of only 2-beams or a single systematic row to calculate the image contrast, or calculating the image using a column approximation. The increasing amount of literature showing comparisons of experimental and simulated images shows that it is possible to obtain very close agreement between the two images; although the choice of parameters used, and the assumptions made, in performing the calculation must be properly dealt with. The simulation of the images of defects in materials has, in many cases, therefore become a tractable problem.

Sign in / Sign up

Export Citation Format

Share Document