scholarly journals An Inexpensive, Whole-plant, Open Gas-exchange System for the Measurement of Photosynthesis in Potted Woody Plants

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 779E-779
Author(s):  
David P. Miller ◽  
G. Stanley Howell ◽  
James A. Flore
Keyword(s):  
Gas Exchange ◽  
Abiotic Factors ◽  
Incident Radiation ◽  
Single Leaf ◽  
Whole Plant ◽  
Plant Assimilation ◽  
Gas Exchange System ◽  
Source Sink ◽  
Short Time

The measurement of whole-plant CO2 uptake integrates leaf-to-leaf variability, which arises from such sources as angle of incident radiation, source/sink relationships, age, and biotic or abiotic factors. Respiration of above-ground vegetative and reproductive sinks is also integrated into the final determination of whole-plant CO2 assimilation. While estimates of whole-plant CO2 uptake based on single-leaf determinations have been used, they do not accurately reflect actual whole-plant assimilation. Chambers were constructed to measure gas exchange of entire potted grapevines. The design and construction are simple, inexpensive, and easy to use, allowing for the measurement of many plants in a relatively short time. This enables the researcher to make replicated comparisons of the whole-plant CO2 assimilation of various treatments throughout the growing season. While CO2 measurement was the focus of this project, it is also possible to measure whole-plant transpiration with this system.

scholarly journals A whole canopy gas exchange system for the targeted manipulation of grapevine source-sink relations using sub-ambient CO2

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Jason P. Smith ◽  
Everard J. Edwards ◽  
Amanda R. Walker ◽  
Julia C. Gouot ◽  
Celia Barril ◽  
...  
Keyword(s):  
Gas Exchange ◽  
Leaf Area ◽  
Canopy Structure ◽  
Experimental System ◽  
Soda Lime ◽  
Sugar Accumulation ◽  
Wine Quality ◽  
Gas Exchange System ◽  
Berry Composition ◽  
Source Sink

Abstract Background Elucidating the effect of source-sink relations on berry composition is of interest for wine grape production as it represents a mechanistic link between yield, photosynthetic capacity and wine quality. However, the specific effects of carbohydrate supply on berry composition are difficult to study in isolation as leaf area or crop adjustments can also change fruit exposure, or lead to compensatory growth or photosynthetic responses. A new experimental system was therefore devised to slow berry sugar accumulation without changing canopy structure or yield. This consisted of six transparent 1.2 m3 chambers to enclose large pot-grown grapevines, and large soda-lime filled scrubbers that reduced carbon dioxide (CO2) concentration of day-time supply air by approximately 200 ppm below ambient. Results In the first full scale test of the system, the chambers were installed on mature Shiraz grapevines for 14 days from the onset of berry sugar accumulation. Three chambers were run at sub-ambient CO2 for 10 days before returning to ambient. Canopy gas exchange, and juice hexose concentrations were determined. Net CO2 exchange was reduced from 65.2 to 30 g vine− 1 day− 1, or 54%, by the sub-ambient treatment. At the end of the 10 day period, total sugar concentration was reduced from 95 to 77 g L− 1 from an average starting value of 23 g L− 1, representing a 25% reduction. Scaling to a per vine basis, it was estimated that 223 g of berry sugars accumulated under ambient supply compared to 166 g under sub-ambient, an amount equivalent to 50 and 72% of total C assimilated. Conclusions Through supply of sub-ambient CO2 using whole canopy gas exchange chambers system, an effective method was developed for reducing photosynthesis and slowing the rate of berry sugar accumulation without modifying yield or leaf area. While in this case developed for further investigations of grape and wine composition, the system has broader applications for the manipulation and of study of grapevine source-sink relations.

scholarly journals A Whole-plant, Open, Gas-exchange System for Measuring Net Photosynthesis of Potted Woody Plants

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 944-946 ◽  
Author(s):  
D.P. Miller ◽  
G.S. Howell ◽  
J.A. Flore
Keyword(s):  
Gas Exchange ◽  
Photosynthetically Active Radiation ◽  
Woody Plants ◽  
Net Photosynthesis ◽  
Growing Season ◽  
Exchange System ◽  
Active Radiation ◽  
Whole Plant ◽  
Short Period ◽  
Gas Exchange System

Chambers were constructed to measure gas exchange of entire potted grapevines (Vitis vinifera L.). The plant enclosures were constructed from Mylar film, which is nearly transparent to photosynthetically active radiation. Maintaining a slight, positive, internal pressure allowed the Mylar chambers to inflate like balloons and required no other means of support. The whole-plant, gas-exchange chamber design and construction were simple and inexpensive. They were assembled easily, equilibrated quickly, and did not require cooling. They allowed for the measurement of many plants in a relatively short period. This system would enable the researcher to make replicated comparisons of treatment influences on whole-plant CO2 assimilation throughout the growing season. While CO2 measurement was the focus of this project, it would be possible to measure whole-plant transpiration with this system.

Determination of factors involved in the rejection of bananas (Musa acuminata) intended for international commercialization

2020 ◽  
Keyword(s):  
Management Practices ◽  
Abiotic Factors ◽  
Quality Standards ◽  
Musa Acuminata ◽  
P Value ◽  
Biotic Factors ◽  
Significance Level ◽  
Export Sector ◽  
Export System

The banana agro-export sector in Ecuador provides millions of dollars in income for this concept, but with this development, a series of quality standards have been established that must be met to enter the export system. This has contributed to establishing good post-harvest production and management practices that guarantee the optimal production of bananas and plantains. The objective of this study was to determine the factors involved in the rejection of bananas (Musa acuminata) destined for international commercialization. The methodology considered the design modality of non-experimental transactional research, with a quantitative approach. The methodological design was developed in three phases at Finca 6 Hermanas located in the Barraganete sector of the San Juan parish in the Puebloviejo canton of the Los Ríos Province, Ecuador. The results highlight that the main causes for which banana rejection is generated are due to abiotic factors (damage, dry latex, scar, insect damage, broken neck, overgrowth) in a higher percentage of 79.55 % and biotic factors ( twins, diseases, short finger) by 20.45 %. The average rejection was 6 361 fingers and1 269 Kilograms (K) over the 6-week study duration. The analysis of variance turned out to be significant for variable 1 (biotic and abiotic). Ho is rejected; with the criterion of p-value < 0.0001 and F (9; 45) = 2.10., F = 13.17> F critic. In the case of variable (2) “work weeks”, Ho is accepted with the criteria obtained of p-value of 0.7694 and F (5; 45) = 2.4., As F = 0.51 < F critic, it is concludes, that with a significance level of 5% the null hypothesis is accepted. It is concluded that these figures lead to the elaboration of strategies that systemically mitigate the damages, by correcting each one of the causes that cause the deterioration of the banana and increasing the economic gains of the commercialization process.

scholarly journals Expression Level of Mature miR172 in Wild Type and StSUT4-Silenced Plants of Solanum tuberosum Is Sucrose-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn
Keyword(s):  
Solanum Tuberosum ◽  
Wild Type ◽  
Potato Plants ◽  
Mature Leaves ◽  
In Vitro Plantlets ◽  
Long Time ◽  
Short Time ◽  
Cut Leaves

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.

A new automated stem CO2 efflux chamber based on industrial ultra-low-cost sensors

2019 ◽  
Author(s):  
Johannes Brändle ◽  
Norbert Kunert
Keyword(s):  
Gas Exchange ◽  
Low Cost ◽  
Climatic Conditions ◽  
Co2 Efflux ◽  
Diurnal Changes ◽  
Lowland Forest ◽  
Stem Respiration ◽  
Stem Co2 Efflux ◽  
Gas Exchange System ◽  
Controlling Processes

Abstract Tree autotrophic respiratory processes, especially stem respiration or stem CO2 efflux (Estem), are important components of the forest carbon budget. Despite the efforts to investigate the controlling processes of Estem in the last years a considerable lack in our knowledge remains on the abiotic and biotic drivers affecting Estem dynamics. It has been strongly advocated that long-term measurements would shed light into those processes. The expensive scientific instruments needed to measure gas exchange has prevented from applying Estem measurements on a larger temporal and spatial scale. Here, we present an automated closed dynamic chamber system based on inexpensive and industrially broadly applied CO2 sensors reducing the costs for the sensing system to a minimum. The CO2 sensor was cross-calibrated with a commonly used gas exchange system in the laboratory and in the field, and we found very good accordance of these sensors. We tested the system under harsh tropical climatic conditions, characterized by heavy tropical rainfall events, extreme humidity, and temperatures, in a moist lowland forest in Malaysia. We recorded Estem of three Dyera costulata trees with our prototype over various days. The variation of Estem was large among the three tree individuals and varied by 7.5-fold. However, clear diurnal changes in Estem were present in all three tree individuals. One tree showed high diurnal variation in Estem and the relationship between Estem and temperature was characterized by a strong hysteresis. The large variations found within one single tree species highlights the importance of continuous measurement to quantify ecosystem carbon fluxes.

scholarly journals Normal Cyclic Variation in CO2 Concentration in Indoor Chambers Decreases Leaf Gas Exchange and Plant Growth

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 663
Author(s):  
James Bunce
Keyword(s):  
Stomatal Conductance ◽  
Gas Exchange ◽  
Leaf Gas Exchange ◽  
Genetic Material ◽  
Co2 Concentration ◽  
Cyclic Variation ◽  
Indoor Exposure ◽  
Elevated Atmospheric Co2 ◽  
Gas Exchange System ◽  
Opening And Closing

Attempts to identify crop genetic material with larger growth stimulation at projected elevated atmospheric CO2 concentrations are becoming more common. The probability of reductions in photosynthesis and yield caused by short-term variation in CO2 concentration within elevated CO2 treatments in the free-air CO2 enrichment plots raises the question of whether similar effects occur in glasshouse or indoor chamber experiments. These experiments were designed to test whether even the normal, modest, cyclic variation in CO2 concentration typical of indoor exposure systems have persistent impacts on photosynthesis and growth, and to explore mechanisms underlying the responses observed. Wheat, cotton, soybeans, and rice were grown from seed in indoor chambers at a mean CO2 concentration of 560 μmol mol−1, with “triangular” cyclic variation with standard deviations of either 4.5 or 18.0 μmol mol−1 measured with 0.1 s sampling periods with an open path analyzer. Photosynthesis, stomatal conductance, and above ground biomass at 20 to 23 days were reduced in all four species by the larger variation in CO2 concentration. Tests of rates of stomatal opening and closing with step changes in light and CO2, and tests of responses to square-wave cycling of CO2 were also conducted on individual leaves of these and three other species, using a leaf gas exchange system. Reduced stomatal conductance due to larger amplitude cycling of CO2 during growth occurred even in soybeans and rice, which had equal rates of opening and closing in response to step changes in CO2. The gas exchange results further indicated that reduced mean stomatal conductance was not the only cause of reduced photosynthesis in variable CO2 conditions.

scholarly journals Delayed Senescence in Soybean: Terminology, Research Update, and Survey Results from Growers

2016 ◽  
Vol 17 (2) ◽  
pp. 76-83 ◽  
Author(s):  
C. J. Harbach ◽  
T. W. Allen ◽  
C. R. Bowen ◽  
J. A. Davis ◽  
C. B. Hill ◽  
...  
Keyword(s):  
Abiotic Factors ◽  
Biotic Factors ◽  
Delayed Senescence ◽  
Plant Parts ◽  
Whole Plant ◽  
Research Findings ◽  
Survey Results ◽  
Soybean Plants ◽  
Grower Survey ◽  
Specific Symptoms

The terms used to describe symptoms of delayed senescence in soybean often are used inconsistently or interchangeably and do not adequately distinguish the observed symptoms in the field. Various causes have been proposed to explain the development of delayed senescence symptoms. In this article, we review published reports on delayed senescence symptoms in soybean, summarize current research findings, provide examples of terms related to specific symptoms, and present an overview of the results of a multi-state survey directed to soybean growers to understand their concerns about delayed soybean senescence. Some of these terms, such as green bean syndrome and green stem syndrome, describe symptoms induced by biotic factors, while other terms describe symptoms associated with abiotic factors. Some delayed senescence terms involve the whole plant remaining green while other terms include just the stem and other plant parts such as pods. In the grower survey, 77% reported observing soybean plants or plant parts that remained green after most plants in the field were fully mature with ripe seed. Most respondents attributed these symptoms to changes in breeding and choice of cultivars. At the end of this article, we standardized the terms used to describe delayed senescence in soybean. Accepted for publication 23 March 2016. Published 15 April 2016.

scholarly journals Dual 2-Hydroxypropyl-β-Cyclodextrin and 5,10,15,20-Tetrakis (4-Hydroxyphenyl) Porphyrin System as a Novel Chiral-Achiral Selector Complex for Enantioseparation of Aminoalkanol Derivatives with Anticancer Activity in Capillary Electrophoresis

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 993
Author(s):  
Błażej Grodner ◽  
Mariola Napiórkowska
Keyword(s):  
Capillary Electrophoresis ◽  
Anticancer Agents ◽  
Chiral Discrimination ◽  
Combined Effects ◽  
Pilot Studies ◽  
Repeatability And Reproducibility ◽  
The Stability ◽  
Short Time ◽  
High Separation

In this study, a complex consisting of 2-hydroxypropyl-β-cyclodextrin and 5,10,15,20-tetrakis (4-hydroxyphenyl) porphyrin, (named dual chiral-achiral selector complex) was used for the determination of two novel potential anticancer agents of (I) and (II) aminoalkanol derivatives. This work aimed at developing an effective method that can be utilized for the determination of I (S), I (R), and II (S) and II (R) enantiomers of (I) and (II) compounds through the use of a dual chiral-achiral selector complex consisting of hydroxypropyl-β-cyclodextrin and 5,10,15,20-tetrakis (4-hydroxyphenyl) porphyrin system by applying capillary electrophoresis. This combination proved to be beneficial in achieving high separation selectivity due to the combined effects of different modes of chiral discrimination. The enantiomers of (I) and (II) compounds were separated within a very short time of 3.6–7.2 min, in pH 2.5 phosphate buffer containing 2-hydroxypropyl-β-cyclodextrin and 5,10,15,20-tetrakis (4-hydroxyphenyl) porphyrin system at a concentration of 5 and 10 mM, respectively, at 25 °C and +10 kV. The detection wavelength of the detector was set at 200 nm. The LOD for I (S), I (R), II (S), and II (R) was 65.2, 65.6, 65.1, and 65.7 ng/mL, respectively. LOQ for I (S), I (R), II (S), and II (R) was 216.5, 217.8, 217.1, and 218.1 ng/mL, respectively. Recovery was 94.9–99.9%. The repeatability and reproducibility of the method based on the values of the migration time, and the area under the peak was 0.3–2.9% RSD. The stability of the method was determined at 0.1–4.9% RSD. The developed method was used in the pilot studies for determining the enantiomers I (S), I (R), II (S), and II (R) in the blood serum.

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