scholarly journals Timing and K-IBA Treatments Affect Rooting of Stem Cuttings of Cephalotaxus harringtonia

HortScience ◽  
1996 ◽  
Vol 31 (2) ◽  
pp. 222-223 ◽  
Author(s):  
Andrea L. Southworth ◽  
Michael A. Dirr

Stem cuttings from a prostrate clone of Cephalotaxus harringtonia (Forbes) K. Koch (Japanese plum yew) were taken monthly from Sept. 1994 through Aug. 1995, treated with K-IBA at 0 or 10,000 mg·liter–1, placed in a greenhouse under intermittent mist, and evaluated after 16 weeks. Cuttings taken from December to February and treated with K-IBA averaged 85% rooting, 10 roots per cutting, and a total root length of 35 cm. The next highest rooting percentages were for cuttings taken from March to May; poorest rooting occurred for cuttings taken from June to August and September to November, regardless of K-IBA application. Chemical name used: K-indole-3-butyric acid (K-IBA).

2012 ◽  
Vol 22 (4) ◽  
pp. 476-478
Author(s):  
Eugene K. Blythe

Confederate rose (Hibiscus mutabilis), a native of southeastern China, is an old-fashioned, ornamental plant often found in older gardens in the southern United States. Current breeding programs aim at developing selections with improved garden performance, thus providing new cultivars for nursery production. Hardy in U.S. Department of Agriculture (USDA) zones 7 to 9, plants grow as large shrubs or small trees in warmer areas, but generally die back to a woody base or short trunk in colder areas of their range. Stems from the past growing season that remain on plants during the winter in the warmer regions may be used to prepare hardwood stem cuttings. The current study examined hardwood cutting propagation of confederate rose in response to a 1-second basal quick-dip in auxin [1000 ppm indole-3-butyric acid (IBA), 3000 ppm IBA, 1000 ppm IBA + 500 ppm 1-naphthaleneacetic acid (NAA), and 3000 ppm IBA + 1500 ppm NAA] and a basal wound (along with 1000 ppm IBA only). Cuttings were rooted in a warm, high-humidity environment within a greenhouse. Auxin treatments improved overall rooting percentage and total root length, with 1000 ppm IBA (without and with a basal wound) providing the highest rooting percentages (about 70%) and nontreated cuttings the lowest (44%). A significant increase in total root length on rooted cuttings resulted with the use of 3000 ppm IBA (211 cm) and use of a basal wound plus 1000 ppm IBA (193 cm) compared with nontreated cuttings (87 cm). Auxin and wounding treatments did not have any significant inhibitory effects on budbreak and growth of new shoots on rooted cuttings.


2004 ◽  
Vol 14 (4) ◽  
pp. 479-483 ◽  
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
Ken M. Tilt ◽  
John M. Ruter

In five experiments, singlenode cuttings of `Red Cascade' miniature rose (Rosa) were treated with a basal quick-dip (prior to insertion into the rooting substrate) or sprayed to the drip point with a single foliar application (after insertion) of Dip `N Grow [indole-3-butyric acid (IBA) + 1-naphthaleneacetic acid (NAA)], the potassium salt of indole-3-butyric acid (K-IBA), or the potassium salt of 1-naphthaleneacetic acid (K-NAA); a single foliar spray application of Dip `N Grow with and without Kinetic surfactant; or multiple foliar spray applications of Dip `N Grow. Spray treatments were compared with their respective basal quick-dip controls {4920.4 μm [1000 mg·L-1 (ppm)] IBA + 2685.2 μm (500 mg·L-1) NAA, 4144.2 μm (1000 mg·L-1) K-IBA, or 4458.3 μm (1000 mg·L-1) K-NAA}. Cuttings sprayed with 0 to 246.0 μm (50 mg·L-1) IBA + 134.3 μm (25 mg·L-1) NAA, 0 to 207.2 μm (50 mg·L-1) K-IBA, or 0 to 222.9 μm (50 mg·L-1) K-NAA resulted in rooting percentages, total root length, percent rooted cuttings with shoots, and shoot length similar to or less than control cuttings. Exceptions were cuttings sprayed with 0 to 2.23 μm(0.5 mg·L-1) K-NAA, which exhibited shoot length greater than the control cuttings. Addition of 1.0 mL·L-1 (1000 ppm) Kinetic organosilicone surfactant to spray treatments resulted in greater total root length and shoot length. Repeated sprays (daily up to seven consecutive days) had no or negative effects on root and shoot development.


1989 ◽  
Vol 67 (7) ◽  
pp. 2168-2174 ◽  
Author(s):  
T. J. Tschaplinski ◽  
T. J. Blake

The relationship between the rooting patterns of five poplar hybrids and stem carbohydrate dynamics was investigated to determine if the availability or metabolism of carbohydrates was limiting early root production of cuttings. Differences in early root production (i.e., number, length, and dry weight) after 17 days paralleled the subsequent differences in accumulation of aboveground biomass that were observed when two Populus balsamifera L. × deltoides Bartr. (Jackii 4 and Jackii 7) and three Populus deltoides Bartr. × nigra L. (DN 2, DN 22, and DN 15) hybrids were compared. Total root length and dry weight of Jackii 4 was four times that of Jackii 7. Both DN 2 and DN 22 had a total root length twice that of DN 15. Rooting characteristics may be useful criteria for selection of productive clones. Carbohydrate analysis of stem cuttings suggested that hybrids differing in biomass production displayed different patterns of carbohydrate utilization. The concentrations of glucose and myoinositol declined more in faster growing hybrids (Jackii 4, DN 2, DN 22) than in slower growing hybrids (Jackii 7, DN 15). The results suggest that faster growing hybrids consume monosaccharides in growth processes, whereas the slower growing hybrids convert monosaccharides into alternative storage forms and use less in growth processes. The initial myoinositol concentration in stem cuttings of poplar hybrids was correlated (r2 = 0.95) with root dry weight produced after 17 days, but the initial starch concentration was unrelated to later root biomass production. The results suggest that initial myoinositol concentration in cuttings may be used in early selection for rooting performance.


2021 ◽  
Vol 5 (47) ◽  
pp. 22-22
Author(s):  
Maxim Simakhin ◽  
Yulia Dotsenko ◽  
Tatiana Aniskina ◽  
Vitaly Donskikh ◽  
Inna Zubik ◽  
...  

In this work, the results of evaluating the differences in the variants of the three-factor experiment on rooting of varieties of Barberry Thunberg (Berberis thunbergii DC.) By factors: varietal characteristics, the number of nodes and the use of a root formation regulator are considered. The study involved cuttings of the following varieties: ‘Dart’s Red Lädy’, ‘Kobold’, ‘Golden Ring’, ‘Harlequin’, ‘Rosetta’, ‘Red Chief’ and ‘Aurea’. 4- (indol-3-yl) butyric acid (trademark "Kornevin") was used as a root formation stimulator by the method of dusting. The green cuttings had 2 and 3 knots. The results of analysis of variance on the variability of the average total root length in green cuttings depending on varietal characteristics, dusting with a root formation stimulator and the number of nodes made it possible to establish that the ‘Red Chief’ variety had the maximum average total root length and was significantly different from all other varieties. The average total length of roots when using a root formation stimulator is significantly higher than in the control variant. Cuttings with three nodes reliably had a large average total root length. Based on the results obtained, it is assumed that the use of a root formation stimulator and rooting of cuttings with three nodes has a positive effect on the rooting processes of barberry cuttings. Keywords: BERBERIS THUNBERGII, THUNBERG BARBERRY, GREEN CUTTINGS, 4- (INDOL-3-YL) BUTYRIC ACID, KORNEVIN, KNOTS


2000 ◽  
Vol 10 (4) ◽  
pp. 780-784
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.


2017 ◽  
Vol 27 (1) ◽  
pp. 69-72
Author(s):  
Justin A. Schulze ◽  
Ryan N. Contreras ◽  
Carolyn F. Scagel

‘Schipkaensis’ common cherrylaurel (Prunus laurocerasus) is an important nursery crop across the United States. In our breeding efforts to reduce shot-hole symptoms and weediness, we have created chromosome doubled forms of this cultivar. Vegetative propagation is an important factor in nursery production, and we have found no studies that have looked at comparative adventitious rooting of stem cuttings using induced polyploids. The objective of this research was to determine if rooting ability varied between these two ploidy levels. Semihardwood stem cuttings from wild-type (22x) and polyploid (44x) ploidy levels were taken at the end of July 2015 and the beginning of July 2016. Cuttings were dipped in 1030 ppm (0.10%) indole-3-butyric acid (IBA) and 660 ppm (0.066%) 1-naphthaleneacetic acid (NAA) before being set in rooting substrate. After 1 month, cuttings were removed from substrate and data collected. Data included; rooting percentage, root number per rooted cutting, average root length, and total root length. In 2015, 88% of the cuttings from the 44x plants and 63% of the cuttings from the 22x plants rooted. In 2016, 100% of cuttings from both ploidy levels rooted. In both years, average root length and total root length were similar between ploidy levels; however, cuttings from 22x plants generally had more roots than those from 44x. Chromosome-doubled ‘Schipkaensis’ common cherrylaurel rooted effectively, and produce transplantable cuttings similar to the standard ploidy.


2017 ◽  
Vol 14 (2) ◽  
pp. 124-129 ◽  
Author(s):  
Mohammed Enamul Hoque

A field experiment was conducted at the central nursery of tea extension center of Bangladesh Tea Board in Bandarban during September to November, 2015 to determine the precise concentration of Indole Butyric Acid (IBA) on propagation through stem cuttings of tea. The experiment was laid out in a randomized block design with four treatments of IBA concentrations: 0 ppm (Control), 2000 ppm, 4000 ppm and 6000 ppm and each treatment was replicated four times. Basal part of tea (BT-2 variety) cuttings were dipped in different IBA concentrations for five seconds. Then these cuttings were dibbled into prepared soil mixture as rooting media. Cuttings were exposed to the rooting media for 90 days and then they all were removed carefully to assess the variation of establishment of cuttings. IBA treated cuttings showed better effect on the rooting rate, root length, root number, shoot length and survival rate compared to the control. It was noted that cuttings treated with 4000 ppm IBA produced the highest rooting rate 79.85%, the highest survival rate 57.60% and the highest root length of 4.85 cm followed by treatment 6000 ppm IBA. In case of all parameters, effects of the treatments 4000 ppm and 6000 ppm of IBA were statistically similar. Positive and strong correlation was observed both in rooting rate versus survival rate and root length versus shoot length in IBA treated cuttings. Thus, IBA at 4000 ppm concentration was recommended for establishment of stem cuttings of tea (BT-2 variety) in the nursery.The Agriculturists 2016; 14(2) 124-129


2009 ◽  
Vol 19 (1) ◽  
pp. 130-132 ◽  
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley

‘Dwarf Burford’ holly (Ilex cornuta ‘Dwarf Burford’) is a significant nursery crop and is widely used in landscapes in U.S. Department of Agriculture hardiness zones 7 to 9. Stem cuttings can be rooted at multiple times during the year, provided cutting wood is sufficiently mature, with auxin treatments traditionally used to encourage rooting. This study was conducted to determine if auxin treatment could be eliminated, thus reducing labor and chemical requirements in the cutting propagation process. In three experiments, terminal stem cuttings of ‘Dwarf Burford’ holly were taken in winter, prepared with and without use of a basal quick-dip in an auxin solution, and rooted in a warm, high-humidity environment. Rooting percentages for nontreated cuttings and cuttings treated with 2500 ppm indole-3-butyric acid (IBA) + 1250 ppm 1-naphthaleneacetic acid (NAA) were similar, while treatment of cuttings with 5000 ppm IBA + 2500 ppm NAA resulted in a decrease in rooting percentage. The number of primary roots and total root length were similar among the three treatments, except in one experiment where total root length was greater with auxin-treated cuttings than with nontreated cuttings. Initial shoot growth responses were variable among the three experiments. The treatment of cuttings with auxin was not required for successful rooting and can be eliminated from the process for winter stem cutting propagation of ‘Dwarf Burford’ holly.


HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 771-777 ◽  
Author(s):  
Christopher L. Rosier ◽  
John Frampton ◽  
Barry Goldfarb ◽  
Farrell C. Wise ◽  
Frank A. Blazich

Two experiments were conducted to develop a protocol for rooting stem cuttings from 3-, 5-, and 7-year-old fraser fir [Abies fraseri (Pursh) Poir.] Christmas trees. The first experiment tested the effect of stumping treatments and tree age on shoot production and subsequent adventitious rooting. One auxin concentration [4 mm indole-3-butyric acid (IBA)] and a nonauxin control were tested. Stock plants were stumped to the first whorl (trees in the field 3 and 5 years) or the first, third, and fifth whorls (trees in the field 7 years). Intact (nonstumped) controls were also included for each age. The second experiment was designed to create a quantitative description of the effects that crown (foliage and above ground branches of a tree) position have on the rooting of stem cuttings collected from stumped and nonstumped trees. The exact position was determined by measuring the distance from the stem, height from the ground, and the degrees from north. Crown positions were recorded as cuttings were collected and then cuttings were tested for rooting response. The rooting traits assessed in both experiments included rooting percentage, percent mortality, number of primary roots, total root length, root symmetry, and root angle. In the first experiment, rooting percentage, primary root production, and total root length increased as the age of the stock plant decreased and the severity of the stumping treatment increased. Auxin treatment significantly increased rooting percentage, root production, root lengths, and root symmetry while decreasing mortality. Overall, the highest rooting percentages (51%) and the greatest number of primary roots (8.1) occurred when 3-year-old stock plants were stumped to the first whorl and treated the cuttings with 4 mm IBA. The greatest total root lengths (335 mm) occurred in cuttings from the 3-year-old stock plants. In the second experiment, rooting percentage was significantly affected by the position from which the cuttings were collected. Cuttings collected lower in the crown and closer to the main stem rooted more frequently than cuttings collected from the outer and upper crown.


2020 ◽  
Vol 12 (3) ◽  
pp. 260-263
Author(s):  
A. Saeed ◽  
T. Amin

Abstract. The research was conducted in 2018 in order to investigate the effect of location, tree gender and indole butyric acid (IBA) 4000ppm on rooting of semi-hardwood stem cuttings. Two locations (Al-Samrah and Al-Nabaeen) in Latakia province, Syria, different in latitude and above sea level (500 m and 827 m, respectively) were studied. The results showed significant effect (p<0.05) of the location and tree gender on rooting capacity. No rooting was observed in control (without IBA treatment). Location also affected the number of roots/cuttings. No effect of location or tree gender on root length and diameter was established. The highest rooting rates (%) were for cuttings taken from female trees from Al-Samrah location when treated with IBA 4000 ppm (82.2%).


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