355 Effect of Season on Nitrate Accumulation in Hydroponic Lettuce
Serial plantings of hydroponic lettuce were grown throughout the year in the northeast United States to determine how sunlight intensity and solution nitrate affect nitrate in leaf tissue. Two nutrient solutions were used. All essential elements were supplied at the same concentration, except nitric acid was added to the high-N treatment to increase nitrate to 5.7 mm (352 ppm), compared to 4.0 mm (248 ppm) in the low-N treatment. A feedback control system maintained a constant conductivity and volume in the recirculating nutrient solution. The actual nitrate concentration in solution was higher in winter than in summer. In winter, it rose to 800 ppm in the high-N solution, while it remained below 200 ppm in the low-N solution. In summer, nitrate was 200 to 400 ppm in the high-N solution, compared to 40 to 120 ppm in the low-N solution. Concentration of other mineral elements remained at levels similar to the original formulation. Nitrate concentration in leaf tissue when the lettuce plants reached a marketable size was sensitive to sunlight and nitrate supply. In spring and summer, tissue nitrate was as low as 1100 ppm. It increased to about 4000 ppm in lettuce grown in mid-winter in a shaded greenhouse and fed high-N solution, while low-N plants had less than 3000 ppm nitrate. Tissue nitrate was related to solution nitrate. Tissue nitrate increased in proportion to solution nitrate, up to about 400 ppm nitrate in solution, then leveled off at a concentration of about 4000 ppm in the leaves, a relation that was the same under all sunlight intensities. The accumulation of nitrate in the nutrient solution was one cause of the high concentration of nitrate in lettuce leaves.