Genotypic Variation in Senescence and Water Relations in Cut Flowers of Anthurium andraeanum (Hort.)

The role of water status in determining vase life was investigated in three Anthurium andraeanum Hort. cultivars: Spirit, Success, and Honduras. The cultivars were selected based on their vase life in previous studies and designated as ‘Vshort’, ‘Vmed’ and ‘Vlong’ respectively. A timeline experiment observing spadix necrosis (bloom degradation) under controlled conditions determined end of vase life as 15, 18, and 36 days for Vshort, Vmed and Vlong respectively. Spathe relative water content (RWC) was closely associated with bloom degradation with all three cultivars reaching end of vase life at ≈75% spathe RWC. Membrane integrity of the spathe showed no association with bloom degradation before the end of vase life with increased ion leakage found only after spadix necrosis was visible, indicating that bloom degradation was driven by spathe water status rather than senescence induced by other factors. RWC of the peduncle base and apex showed no association with bloom degradation in any of the cultivars. In fact, base and apex RWC remained high throughout the experiment despite the consistent loss of spathe RWC. This suggests that the reduced water content of the spathe was not induced by reduced hydraulic conductance of the peduncle. Stomatal conductance (gS) was highest in Vshort (approximately twice that of Vlong) and likely contributed to the rapid loss of spathe RWC in this cultivar. However, Vmed and Vlong had similar gS rates and water uptake rates despite large differences in spathe RWC. Thus, the two cultivars differed in their ability to retain water within the spathe tissue. In all three cultivars, end of vase life was determined by spathe water status. Genotypic variation in vase life was not driven by differences in the hydraulic conductance of the peduncle as previously thought. Differences in spathe water status were partially explained by differences in gS but other factors were also involved.

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Hydraulic Conductance in Tepal Growth and Extension of Vase Life with Trehalose in Cut Tulip Flowers

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.130.2.275 ◽

2005 ◽

Vol 130 (2) ◽

pp. 275-286 ◽

Cited By ~ 6

Author(s):

Hiroshi Wada ◽

Mari Iwaya-Inoue ◽

Mitsuru Akita ◽

Hiroshi Nonami

Keyword(s):

Hydraulic Conductivity ◽

Growth Rates ◽

Cell Elongation ◽

Room Temperature ◽

Water Status ◽

Hydraulic Conductance ◽

Vase Life ◽

Cut Flowers ◽

Tulipa Gesneriana ◽

Intact Plants

Two cultivars of tulip (Tulipa gesneriana L.) were used to check the effect of trehalose-feeding on longevity of vase life. `Oxford' plants were grown from bulbs, and trehalose-fed cut flowers were compared with the intact plants grown in pots. `Pink Diamond' flowers were obtained commercially as cut flowers from the market, and trehalose-feeding was examined by using only flower parts. In both cultivars of plants, it was confirmed that trehalose-feeding enhanced longevity of the vase life significantly at room temperature. Additionally, mechanisms of prolonging the vase life with trehalose-fed flowers were studied by comparing the water status in the zone of elongation of tulip tepals when their growth rates were modified with different treatments. In the elongating region of tulip tepals, cell elongation rates were linearly correlated to sizes of the growth-induced water potential regardless of treatments. It was found that trehalose-feeding reduced the hydraulic conductance, resulting in a decrease in cell elongation rates. Also, trehalose helped to maintain turgor of tepal cells for longer periods. Furthermore, trehalose enhanced pigmentation in tepals, and thus, trehalose is believed to have had a role in altering the metabolism in elongating cells and in reducing hydraulic conductivity in membranes.

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Involvement of Ethylene Synthetic Inhibitors in Regulating the Senescence of Cut Carnations through Membrane Integrity Maintenance

Journal of Horticultural Research ◽

10.2478/johr-2020-0010 ◽

2020 ◽

Vol 28 (1) ◽

pp. 39-48

Author(s):

Fahmy Hassan ◽

Esmat Ali ◽

Ragia Mazrou

Keyword(s):

Nitric Oxide ◽

Membrane Integrity ◽

Stability Index ◽

Vase Life ◽

Cut Flowers ◽

Relative Water ◽

Mda Content ◽

Biochemical Mechanisms ◽

Oxide Synthase ◽

Marketing Chain

AbstractPostharvest senescence is a critical problem of carnation cut flowers, limiting their transportation and subsequent marketing chain. This study was designed to assess whether the application of 1-methylcyclopropene (1-MCP), silver nanoparticles (AgNPs), and nitric oxide (NO) released from donor sodium nitroprusside (SNP) could prolong the vase life of cut carnations through an influence on the physiological and biochemical mechanisms involved in aging process. 1-MCP was used in the concentrations of 0, 100, 200, and 300 mg·m−3; AgNPs in the concentrations of 0, 50, 100, and 150 mg·dm−3; and SNP in the concentrations of 0, 0.1, 0.2, 0.3, and 0.4 mM. All treatments significantly extended the cut flower life compared with untreated flowers, more so with 300 mg·m−3 of 1-MCP, 100 mg·dm−3 of AgNPs, or 0.3 mM of SNP. All these chemical compounds were able to considerably improve the relative water content (RWC), reduce the malondialdehyde (MDA) content and increase the membrane stability index (MSI) in petals and, therefore, maintain the membrane integrity. In addition, they decreased the activities of acetyl-CoA synthetase (ACS) and nitric oxide synthase (NOS) and, hence, depressed the production of ethylene in carnation cut flowers through downregulating the ethylene production, what prolonged the vase life. Altogether, the application of exogenous 1-MCP, AgNPs, or SNP may provide a promising avenue to improve the postharvest performance of carnation cut flowers.

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The Method of Plant Water Status Measurement in Sweet Potato (Ipomoea Batatas (L) Lam.)

Beccariana Botanical Research Bulletin ◽

10.30862/bbrp.v7i1.274 ◽

2010 ◽

Vol 7 (1) ◽

Author(s):

Saraswati Prabawardani

Keyword(s):

Water Content ◽

Sweet Potato ◽

Relative Water Content ◽

Water Status ◽

Plant Water Status ◽

Equilibration Time ◽

Plant Water ◽

Font Size ◽

Potato Leaf ◽

Relative Water

The measurement of plant water status such as leaf water potential (LWP) and leaf relative water content (RWC) is important part of understanding plant physiology and biomass production. Preliminary study was made to determine the optimum amount of leaf abrasion and equilibration time of sweet potato leaf inside the thermocouple psychrometer chambers. Based on the trial, the standard equilibration time curve of a Peltier thermocouple for sweet potato leaf was between 2 and 3 hours. To increase the water vapour conductance across the leaf epidermis the waxy leaf cuticle should be removed or broken by abrasion. The result showed that 4 times leaf rubbings was accepted as the most effective way to increase leaf vapour conductance of sweet potato in the psychrometer chambers. In calculating the leaf relative water content, unstressed water of sweet potato leaves require 4 hours imbibition, whereas water stressed of sweet potato leaves require 5 to 6 hours to reach the saturation time. Either leaf water potential or relative water content can be used as a parameter for plant water status in sweet potato.

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Vase life studies in cut flowers of anthurium (Anthurium andraeanum Lind.) CV. tropical as influenced by organic preservatives

Agricultural Research Journal ◽

10.5958/2395-146x.2021.00042.9 ◽

2021 ◽

Vol 58 (2) ◽

pp. 291-296

Author(s):

R A Roopa ◽

S Y Chandrashekar ◽

K Harishkumar

Keyword(s):

Vase Life ◽

Cut Flowers ◽

Anthurium Andraeanum

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DEHYDRIN PROFILES OF SOME IRANIAN MELON VARIETIES (Cucumis melo L. Merr) UNDER DROUGHT STRE SS CONDITIONS

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2019.6.8 ◽

2019 ◽

Vol 18 (6) ◽

pp. 75-84

Author(s):

Alireza Motallebi-Azar ◽

István Papp ◽

Anita Szegő

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Water Content ◽

Water Status ◽

Field Capacity ◽

Total Protein Content ◽

Severe Drought ◽

Molecular Weights ◽

Potential Marker ◽

Relative Water

Dehydrins are proteins that play a role in the mechanism of drought tolerance. This study aimed at establishing dehydrin profile and accumulation in four local melon varieties of Iran: Mino, Dargazi, Saveii, and Semsori, as well as in a commercial variety Honeydew. Plants were treated with drought stress by adjusting the soil water content to 75, 50, 40, 30 and 20% of field capacity (FC) by withholding water. Water status of plants was monitored based on the seedling fresh weight (FW) and relative water content of leaves (RWC). Total protein content was extracted, then heat-stable protein (HSP) fraction was isolated for each variety and water stress treatment. After SDS-PAGE of HSP, Western blotting analysis was carried out with Anti-dehydrin rabbit (primary) and Goat anti rabbit (secondary) antibodies. ANOVA results showed that with decreasing FC below 75%, FW and RWC decreased, but these changes significantly varied among genotypes. On the basis of FW and RWC data under different drought stress treatments, the following drought-tolerant ranking was established: Mino > Dargazi > Saveii and Honeydew > Semsori, from tolerant to sensitive order. Results of Western blot analysis showed that expression of some proteins with molecular weights of 19–52 kDa was induced in the studied varieties under drought stress (% FC). Expression level of the dehydrin proteins in different varieties was variable and also depending on the drought stress level applied. However, dehydrin proteins (45 and 50 kDa) showed strong expression levels in all varieties under severe drought stress (20% FC). The abundance of dehydrin proteins was higher in tolerant varieties (Mino and Dargazi) than in moderate and drought sensitive genotypes. Consequently, dehydrins represent a potential marker for selection of genotypes with enhanced drought tolerance.

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Relative water content consistently predicts drought mortality risk in seedling populations with different morphology, physiology, and times to death

10.1101/2020.12.08.416917 ◽

2020 ◽

Author(s):

Gerard Sapes ◽

Anna Sala

Keyword(s):

Water Content ◽

Real Time ◽

Pinus Ponderosa ◽

Mortality Risk ◽

Relative Water Content ◽

Large Scale ◽

Water Status ◽

Physiological Indicators ◽

Relative Water ◽

Global Impacts

AbstractPredicted increases in forest drought mortality highlight the need for predictors of incipient drought-induced mortality (DIM) risk that enable proactive large-scale management. Such predictors should be consistent across plants with varying morphology and physiology. Because of their integrative nature, indicators of water status are promising candidates for real time monitoring of DIM, particularly if they standardize morphological differences among plants. We assessed the extent to which differences in morphology and physiology between Pinus ponderosa populations influence time to mortality and the predictive power of key indicators of DIM risk. Time to incipient mortality differed between populations but occurred at the same relative water content (RWC) and water potential (WP). RWC and WP were more accurate predictors of drought mortality risk than other physiological indicators, including non-structural carbohydrate (NSC) content and percent loss of conductivity (PLC). These results highlight that variables related to water status capture critical thresholds during DIM and the associated dehydration processes. Both WP and RWC are promising candidates for large-scale assessments of DIM risk. RWC is of special interest because it allows comparisons across different morphologies and can be remotely sensed. Our results offer promise for real time landscape-level monitoring of DIM and its global impacts.

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Cultivar differences in the deterioration of vase-life in cut-flowers of Anthurium andraeanum is determined by mechanisms that regulate water uptake

Scientia Horticulturae ◽

10.1016/j.scienta.2009.12.005 ◽

2010 ◽

Vol 124 (1) ◽

pp. 102-108 ◽

Cited By ~ 15

Author(s):

W. Elibox ◽

P. Umaharan

Keyword(s):

Water Uptake ◽

Cultivar Differences ◽

Vase Life ◽

Cut Flowers ◽

Anthurium Andraeanum

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EFFECTIVENESS OF POSTHARVEST SOLUTIONS FOR THE CONSERVATION OF CUT Oncidium varicosum (ORCHIDACEAE) INFLORESCENCES

Ciência e Agrotecnologia ◽

10.1590/s1413-70542015000400001 ◽

2015 ◽

Vol 39 (4) ◽

pp. 315-322 ◽

Cited By ~ 2

Author(s):

Claudia Fabrino Machado Mattiuz ◽

Ben-Hur Mattiuz ◽

Teresinha de Jesus Deléo Rodrigues ◽

Kelly Magalhães Marques ◽

Ramilo Nogueira Martins

Keyword(s):

Water Content ◽

Soluble Sugars ◽

Chemical Compounds ◽

Carotenoid Content ◽

Distilled Water ◽

Cut Flowers ◽

Color Intensity ◽

Tropical Plant ◽

Relative Water ◽

Postharvest Life

The orchid Oncidium varicosum is a tropical plant with significant commercial value due to the beauty of its yellow inflorescences. Several authors have observed a reduction in the water content of cut flowers, consequently, wilting and senescence of petals are the main reasons for the termination of the decorative life of cut flowers. In this research, we studied the effects of several chemical compounds on the postharvest life of cut inflorescences of Oncidium varicosum 'Samurai'. These chemicals are as follows: sucrose (5%) plus 8-hidroxiquinoline citrate (8-HQC) (100 mg L-1) plus silver nitrate (AgNO3) (50 mg L-1); sucrose (5%) plus citric acid (75 mg L-1); quaternary ammonia at 30% (50 mg L-1); and sucrose (5%) plus quaternary ammonia at 30% (50 mg L-1). The highest values for average of relative water content, reducing carbohydrates and soluble sugars were recorded in the flowers treated with the sucrose (5%) plus 8-HQC (100 mg L-1) plus AgNO3 (50 mg L-1) solution. A reduction in color intensity occurred at 12 days postharvest in all treatments and the carotenoid content was reduced to the greatest extent in flowers maintained in distilled water. The lowest numbers of open flowers were observed at 12 and 15 days in the distilled water and quaternary ammonium treatments, being that the others treatments favored the anthesis. The Oncidium inflorescences treated with sucrose plus HQC plus AgNO3 demonstrated a higher number of open flowers and reached the longest longevity (22 days).

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Effects of Daily Harvest Time on Postharvest Longevity, Water Relations, and Carbohydrate Status of Selected Specialty Cut Flowers

HortScience ◽

10.21273/hortsci.49.3.297 ◽

2014 ◽

Vol 49 (3) ◽

pp. 297-305

Author(s):

Iftikhar Ahmad ◽

John M. Dole ◽

Frank A. Blazich

Keyword(s):

Vase Life ◽

Harvest Time ◽

Cut Flowers ◽

Weight Changes ◽

Eustoma Grandiflorum ◽

Relative Water ◽

Leaf Relative Water Content ◽

Postharvest Longevity ◽

Carbohydrate Status ◽

Time Of Harvest

Effects of harvest time (morning, noon, or afternoon) on water uptake, fresh weight changes, termination symptoms, leaf relative water content (LRWC), carbohydrate status, and vase life of cut ‘ABC Purple’ lisianthus (Eustoma grandiflorum Salisb.), ‘Double Eagle’ African Gold Coin Series marigold (Tagetes erecta L.), and ‘Deep Red’ Benary’s Giant Series zinnia (Zinnia elegans Jacq.) were studied. For stems of lisianthus harvested and then stored in the dark with the basal ends in water for 2 weeks at 3 ± 1 °C, those harvested at noon (1200 hr to 1300 hr) or in the afternoon (1700 hr to 1800 hr) had longer vase life compared with stems harvested in the morning (0700 hr to 0800 hr). However, stems of lisianthus evaluated without storage had no differences in vase life. Stems of marigold harvested in the afternoon had longer vase life than morning- or noon-harvested stems. Time of harvest had no effect on cut flower longevity of zinnia. However, vase life was considerably shorter for stems of all species when tested after 2 weeks storage compared with freshly harvested stems. Stems of zinnia harvested at noon had lower LRWC than morning- or afternoon-harvested stems. Marigold stems harvested in the afternoon and evaluated without storage had lowest LRWC on Day 7 of vase life. Harvest time or storage did not influence LRWC of lisianthus. Stems of marigold and lisianthus harvested at noon or in the afternoon had higher levels of carbohydrates compared with morning-harvested stems, whereas freshly harvested stems had higher concentrations of glucose and sucrose, which decreased during storage or the vase period. Sucrose concentrations varied more significantly among various tissues than other sugars presumably as a result of translocation during vase life. In summary, carbohydrate status of stems harvested at different times of the day varied greatly and affected postharvest longevity of cut marigold and lisianthus, but not zinnia.

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Capillary Mats Alter the Water Content in Medium during Mist Propagation of Dendranthema

HortScience ◽

10.21273/hortsci.39.3.584 ◽

2004 ◽

Vol 39 (3) ◽

pp. 584-587 ◽

Cited By ~ 1

Author(s):

R.L. Geneve ◽

S.T. Kester ◽

J.W. Buxton

Keyword(s):

Water Content ◽

Water Table ◽

Relative Water Content ◽

Water Status ◽

Internal Water ◽

Relative Water ◽

Reduced Water ◽

Water Contents

A capillary mat-mist system was developed to provide near constant media water contents at differing quantities of mist. Media water contents were reduced by increasing the capillary mat height above a constant water table maintained at bench level. Increased tensions from 0 to 10 cm above the water table reduced water content in Oasis, rockwool, and peat-perlite by 35.4%, 27.6%, and 17.4%, respectively. There was no difference in water content for each medium when the mist quantity ranged between 600 and 1800 mL·m-2·h-1, except when the capillary mat was at 9 cm above the water table and mist volume was 300 mL·m-2·h-1. Chrysanthemum cuttings rooted best when water content was highest regardless of media. Using the peat-perlite medium, water content had the greatest impact on rooting when the mist volume was low (600 mL·m-2·h-1). Relative water content of cuttings was lowest during the first 5 days of sticking and both reduced media water content and mist quantity resulted in the lowest internal water status for the cuttings.

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