Evaluation of Platelet counts and its Indices in Various Clinical Conditions using Automated Hematology Analyzer in a Tertiary care Hospital

ABSTRACT Background and Objective: With the advent of automated hematology analyzer, the use of traditional microscopy of blood film has become limited. The objective of our study was to determine the percentage of peripheral blood smear review in our institution in the era of automation and to identify reasons of manual review. Materials and Methods: This was a prospective audit from January 1, 2015, to January 15, 2015. Consecutive complete blood count (CBC) samples and peripheral smear requests made up the sample size. All age groups and genders were included. CBCs were performed on Sysmex XE-5000. The variables to be analyzed included inpatient and outpatient samples, frequency of peripheral film review, identifying reasons of smear review, and addition of information missed by the automated analyzer. Results: We analyzed 1200 consecutive CBC samples. Peripheral smear was reviewed in 500 (42%) of the cases of which, 241 were inpatient, and 259 were outpatient samples. In 384/500, the findings of hematology analyzer correlated with peripheral smear review. Flags identified included nucleated red blood cells (NRBCs) in 155 (40%), immature white blood cell (WBC) 129 (34%), and atypical lymphocytes 100 (26%). In 23% of the cases, the analyzer missed important findings. The sensitivity of abnormal histogram in our study was 91.3%, while the sensitivity of abnormal parameters was 100%. Conclusion: Peripheral smear review was performed in 42% of the cases. The analyzer identified NRBC, immature WBC precursors, and atypical lymphocytes as the most common abnormality. The information correlated in 77% of the cases.

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Prevalence of Bartonella henselae infection and its diagnosis in diverse clinical conditions in a tertiary care hospital in North India

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2016.11.309 ◽

2016 ◽

Vol 53 ◽

pp. 124-125

Author(s):

R. Chaudhry ◽

P. Kokkayil ◽

A. Gosh ◽

T. Bahadur ◽

K. Kant ◽

...

Keyword(s):

Tertiary Care Hospital ◽

Bartonella Henselae ◽

Tertiary Care ◽

North India ◽

Care Hospital ◽

Clinical Conditions

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Bartonella henselae infection in diverse clinical conditions in a tertiary care hospital in north India

The Indian Journal of Medical Research ◽

10.4103/ijmr.ijmr_1932_16 ◽

2018 ◽

Vol 147 (2) ◽

pp. 189 ◽

Cited By ~ 1

Author(s):

Rama Chaudhry ◽

Prathyusha Kokkayil ◽

Arnab Ghosh ◽

Tej Bahadur ◽

Kamala Kant ◽

...

Keyword(s):

Tertiary Care Hospital ◽

Bartonella Henselae ◽

Tertiary Care ◽

North India ◽

Care Hospital ◽

Clinical Conditions

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Comparison of haemoglobin assessment by HemoCue 301 and automated haematology analyser using flowcytometry among school going children: a one year study at a tertiary care hospital

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20195631 ◽

2019 ◽

Vol 8 (1) ◽

pp. 15

Author(s):

Sarvepalli Vijaya Kumar ◽

Krishnan Ramalingam

Keyword(s):

Venous Blood ◽

Tertiary Care ◽

Mean Value ◽

Screening Methods ◽

Care Hospital ◽

Cross Sectional ◽

Hematology Analyzer ◽

The Mean ◽

Automated Hematology Analyzer ◽

One Year

Background: Anaemia defined as reduction in the concentration in Haemoglobin is one of the key health indicators of health care system of the country. Accurate screening methods are required to estimate the levels of haemoglobin for diagnosing the cause of anaemia. Objectives of the study was to analyze and compare the results of haemoglobin concentrations estimated with automated haematology analyzer and point of care device HemoCue Hb301.Methods: It is a prospective cross-sectional study was conducted for one year after ethical approval. Non fasting capillary and venous blood samples were collected from the selected cases of children and Haemoglobin concentrations were estimated by automated analyzer and HemoCue Hb301 system and the values were noted. Quality control checks were performed for both. Statistical analysis was done using IBM SPSS Version 24.0.Results: Mean Hb% concentration was estimated in 108 children with 44 female and 64 males. The mean value of Automated hematology analyzer (11.965±1.012) was significantly higher when compared with the mean value of HemoCue Hb301 (11.697±1.312) (p=0.002). There was a significantly strong correlation between HemoCue Hb301and Automated hematology analyzer (r-value = 0.732, p <0.0001).Conclusions: The HemoCue is useful in many different settings and remains a widely used method in field settings as it has several advantages and is relatively inexpensive compared with automated haematology analysers. Further studies are needed to better understand potential sources of error in the Hb assessment by HemoCue with the aim to better train phlebotomists and implement appropriate standardised procedures.

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Evaluation of Platelet Counts in the Normal Pregnancy and other Gestational Conditions at Tertiary Care Hospital of Sindh

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i38b32104 ◽

2021 ◽

pp. 106-111

Author(s):

. Palwasha ◽

Kanwal Abbas Bhatti ◽

Fahmida Gul ◽

Sameena Gul Memon ◽

Pashmina Shaikh

Keyword(s):

Platelet Count ◽

Tertiary Care Hospital ◽

Gestational Hypertension ◽

Tertiary Care ◽

Normal Pregnancy ◽

Major Theme ◽

Care Hospital ◽

Hepatic Disorder ◽

Platelet Counts ◽

Proper Management

Background: During gestational period, the most common disorder is hypertension that directly affects the gestation. The frequency of gestational hypertension is increasing day by day and ultimately the pressure is developed on the endothelial wall. Gestational hypertension mostly reduces the platelet counts. Aim of Study: The major theme of this research is evaluating the count of platelet during pregnancy and other gestational conditions. Methodology: A Retrospective research was carried out for the period of 06 months at Gynae and Obstetrics ward at tertiary care hospital of Sindh, Pakistan. Total 104 females were selected with different gestational age and trimester. A questionnaire was filled by all participants that were comprised of demographic data and gestational conditions such as preeclampsia, eclampsia, parity and seizure episodes. Females with highest risk factors diseases such as Diabetes, Hepatic disorder, Anemia, renal disorder and cardio vascular disease were not included in our research. Blood samples were collected from all selected participants for proper platelet count and data was compared with normal ranges of platelet count among the pregnant females. Data was analyzed by using statistical software 24.00 versions. Results: It was observed that 49 patients were having normal pregnancy, 32 had preeclampsia and 23 had eclampsia. 38 participants were first timer & 52 were having second time parity. 58 participants had 2nd trimester of pregnancy. According to condition of anemia, 27 had severe anemic condition whereas 43 had moderate anemic condition. 49 participants had reduced level of platelet count and 17 had very low platelet count. Severity of gestation can be managed through proper management and physician instructions. Hypertension was measured through severity scale, 29 patients had moderate level of hypertension and 22 had severe level of hypertension. 19 participants had very abnormal level of blood count. Conclusion: It was concluded that proper diagnostic test should be conducted on time for proper management of reduced platelet count and there should be proper mass counseling should be conducted in order to overcome the deficiency of platelet count. There should be proper diet and exercise during pregnancy that can manage the condition of hypertension. Severity of gestation can be managed through proper management and physician instructions.

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