<p><em>Burkholderia cepacia</em> (<em>B. cepacia</em>) is one of nine species the <em>Burkholderia cepacia</em> complex, a group of gram-negative, motile, non-spore-forming and rod-shaped bacteria. Contamination by <em>B. cepacia</em> is found in different industrial issues. <em>B. cepacia</em> affect manufacturing process chains by contaminating the working fluids with planktonic cells and biofilms. Because of the opportunistic pathogenicity to plants, animals, humans and and the multi-drug resistance, <em>B. cepacia</em> is difficult to treat. An alternative treatment method could be the use of herbal raw materials, such as essential oils and their active ingredients. This study aims: (i) to identify the antimicrobial potential of essential oils on the growth of four <em>B. cepacia</em> isolates, (ii) to analyse the influence of active ingredients, on planktonic growth and biofilm formation, (iii) to better understand the impact of commercial and naturally biocides to cell agglomeration as a precursor to mature biofilms. Starting with agar dilution method to evaluate the antimicrobial potential of twenty-three essential oils against <em>B. cepacia</em> (<em>Burk_09</em>,<em> Burk_23</em>,<em> Burk_52</em> and <em>Burk_309</em>) isolated from cathodic dip coating systems and the wild type (<em>DSM_7288</em>), it was all ready possible to identify eight essential oils that inhibit the growth of <em>B. cepacia</em>. Serial microdilution was used to determine the minimal inhibitory concentration (MIC) of the essential oils for growth and biofilm formation inhibition of <em>B. cepacia</em>. The MIC of <em>Melaleuca alternifolia</em> and <em>Citrus aurantium dulcis</em> essential oils were tested equally for all strains. Essential oils contain active ingredients against the growth of multi-drug resistant and pathogenic bacteria. From twelve active substances among others, Terpinen-4-ol and Geraniol were identified that inhibited growth and biofilm formation. It is concluded that essential oils and active ingredients have a good antimicrobial potential, demonstrating a possible more environmental-friendly alternative to commercial biocides applying in industrial fluids.</p>