scholarly journals Effects of dietary fish oil on cytochrome P450 3A expression in the liver of SHR/NDmcr-cp (cp/cp) rats, an animal model for metabolic syndrome

2015 ◽  
Vol 2 (3) ◽  
pp. 127-135 ◽  
Author(s):  
Tohru Yamazaki ◽  
Takashi Ohki ◽  
Hiroki Taguchi ◽  
Asami Yamamoto ◽  
Mari Okazaki ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Animal Model ◽  
Cytochrome P450 ◽  
Fish Oil ◽  
Cytochrome P450 3A ◽  
Dietary Fish

scholarly journals Fish and Dietary Fish Oil Are Associated with a Risk of Metabolic Syndrome in Korean Adults (P08-024-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Eunhee Choi ◽  
Seoeun Ahn ◽  
Kyungho Ha ◽  
Hyojee Joung
Keyword(s):  
Metabolic Syndrome ◽  
Fish Oil ◽  
Science Research ◽  
Epidemiological Studies ◽  
Dietary Intakes ◽  
Dietary Recall ◽  
Fisheries Science ◽  
Funding Sources ◽  
Dietary Fish ◽  
Using Data

Abstract Objectives Several epidemiological studies have examined the association between fish and dietary fish oil intake and metabolic syndrome in a population. However, few studies have investigated fish and dietary fish oil intake and its association with the risk of metabolic syndrome in the Korean population. Methods Using data from the 2013–2016 Korea National Health and Nutrition Examination Survey, a total of 14,519 adults (6,135 men and 8,384 women) aged ≥ 19 years were involved in this study. Dietary intakes of fish and fish oil including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were estimated by a 24-h dietary recall. Metabolic syndrome was diagnosed using the National Cholesterol Education Program Adult Treatment Panel III criteria. The odds ratios (ORs) for the presence of metabolic syndrome according to the tertile of fish and fish oil intakes were calculated using a multiple logistic regression model. Results The prevalence of metabolic syndrome among the participants was 9.18%. The mean intake (standard error, SE) of fish, EPA and DHA was 123.35 (2.67) g/day, 103.43 (2.48) mg/day and 179.21 (4.75) mg/day for the study participants, respectively. Dietary intakes of fish and fish oil were not associated with the prevalence of metabolic syndrome, however, they were associated with the prevalence of several metabolic abnormalities. Men in the highest tertile of energy adjusted DHA intake from fish showed a 20% decreased risk of hypertriglyceridemia (OR, 0.80; 95% CI, 0.67–0.96, p for trend, 0.0274), compared with those in the lowest tertile. However, Women in the highest tertile of fish intake showed a higher prevalence of impaired fasting glucose (OR, 1.70; 95% CI, 1.24–2.33) than those in the lowest tertile. Conclusions Our findings suggest that a higher intake of dietary fish oil might be associated with a lower risk of hypertriglyceridemia in Korean men. Thus, further prospective studies are needed to examine the association of fish and fish oil with metabolic syndrome. Funding Sources This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2018R1A2B6007070) and the National Institute of Fisheries Science (R2019051).

scholarly journals Dietary fish oil reduces glomerular injury and elevated renal hydroxyeicosatetraenoic acid levels in the JCR:LA-cp rat, a model of the metabolic syndrome

2012 ◽  
Vol 110 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Harold M. Aukema ◽  
Jing Lu ◽  
Faye Borthwick ◽  
Spencer D. Proctor
Keyword(s):  
Metabolic Syndrome ◽  
Fish Oil ◽  
Potential Role ◽  
Nutritional Intervention ◽  
Glomerular Injury ◽  
Balanced Diet ◽  
The Metabolic Syndrome ◽  
Eicosanoid Metabolism ◽  
Dietary Fish ◽  
Total Fat

We have previously shown nutritional intervention with fish oil (n-3 PUFA) to reduce numerous complications associated with the metabolic syndrome (MetS) in the JCR:LA-corpulent (cp) rat. In the present study, we sought to explore the potential role of fish oil to prevent glomerulosclerosis in JCR:LA-cp rats via renal eicosanoid metabolism and lipidomic analysis. Male lean and MetS JCR:LA-cp rats were fed a lipid-balanced diet supplemented with fish oil (5 or 10 % of total fat). After 16 weeks of feeding, albuminuria was significantly reduced in MetS rats supplemented with 5 or 10 % fish oil ( − 53 and − 70 %, respectively, compared with the untreated MetS rats). The 5 % fish oil diet resulted in markedly lower glomerulosclerosis ( − 43 %) in MetS rats and to a lesser extent in those supplemented with 10 % fish oil. Interestingly, untreated MetS rats had higher levels of 11- and 12-hydroxyeicosatetraenoic acids (HETE) v. lean rats. Dietary fish oil reduced these levels, as well as other (5-, 9- and 15-) HETE. Whilst genotype did not alter prostanoid levels, fish oil reduced endogenous renal levels of 6-keto PGF1α (PGI2 metabolite), thromboxane B2 (TxB2), PGF2α and PGD2 by approximately 60 % in rats fed 10 % fish oil, and TxB2 ( − 50 %) and PGF2α ( − 41 %) in rats fed 5 % fish oil. In conclusion, dietary fish oil prevented glomerular damage in MetS rats and mitigated the elevation in renal HETE levels. These results suggest a potential role for dietary fish oil to improve dysfunctional renal eicosanoid metabolism associated with kidney damage during conditions of the MetS.

scholarly journals Combined Therapy of Dietary Fish Oil and Stearoyl-CoA Desaturase 1 Inhibition Prevents the Metabolic Syndrome and Atherosclerosis

2010 ◽  
Vol 30 (1) ◽  
pp. 24-30 ◽  
Author(s):  
J. Mark Brown ◽  
Soonkyu Chung ◽  
Janet K. Sawyer ◽  
Chiara Degirolamo ◽  
Heather M. Alger ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Fish Oil ◽  
Combined Therapy ◽  
The Metabolic Syndrome ◽  
Dietary Fish ◽  
Stearoyl Coa Desaturase

scholarly journals Proteomic profiling of murine biliary-derived hepatic organoids and their capacity for drug disposition, bioactivation and detoxification

2021 ◽  
Author(s):  
Lawrence Howell ◽  
Rosalind E. Jenkins ◽  
Stephen Lynch ◽  
Carrie Duckworth ◽  
B. Kevin Park ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Drug Metabolism ◽  
Rna Polymerase Ii ◽  
Liver Tissue ◽  
Drug Disposition ◽  
Multiple Drug ◽  
Proteomic Profiling ◽  
Cytochrome P450 3A ◽  
Drug Induced

AbstractHepatic organoids are a recent innovation in in vitro modeling. Initial studies suggest that organoids better recapitulate the liver phenotype in vitro compared to pre-existing proliferative cell models. However, their potential for drug metabolism and detoxification remains poorly characterized, and their global proteome has yet to be compared to their tissue of origin. This analysis is urgently needed to determine what gain-of-function this new model may represent for modeling the physiological and toxicological response of the liver to xenobiotics. Global proteomic profiling of undifferentiated and differentiated hepatic murine organoids and donor-matched livers was, therefore, performed to assess both their similarity to liver tissue, and the expression of drug-metabolizing enzymes and transporters. This analysis quantified 4405 proteins across all sample types. Data are available via ProteomeXchange (PXD017986). Differentiation of organoids significantly increased the expression of multiple cytochrome P450, phase II enzymes, liver biomarkers and hepatic transporters. While the final phenotype of differentiated organoids is distinct from liver tissue, the organoids contain multiple drug metabolizing and transporter proteins necessary for liver function and drug metabolism, such as cytochrome P450 3A, glutathione-S-transferase alpha and multidrug resistance protein 1A. Indeed, the differentiated organoids were shown to exhibit increased sensitivity to midazolam (10–1000 µM) and irinotecan (1–100 µM), when compared to the undifferentiated organoids. The predicted reduced activity of HNF4A and a resulting dysregulation of RNA polymerase II may explain the partial differentiation of the organoids. Although further experimentation, optimization and characterization is needed relative to pre-existing models to fully contextualize their use as an in vitro model of drug-induced liver injury, hepatic organoids represent an attractive novel model of the response of the liver to xenobiotics. The current study also highlights the utility of global proteomic analyses for rapid and accurate evaluation of organoid-based test systems.

Dietary fish oil modulation of macrophagetumoricidal activity

Nutrition ◽  
1996 ◽  
Vol 12 (1) ◽  
pp. S34-S38
Author(s):  
Kent L. Erickson ◽  
Neil E. Hubbard
Keyword(s):  
Fish Oil ◽  
Dietary Fish
Sign in / Sign up

Export Citation Format

Share Document