Low-dose Nicotine Exposure Induced the Oxidative Damage of Reproductive Organs and Altered the Sperm Characteristics of Adolescent Male Rats

he present work was designed to investigate the reproductive toxicity induced by oral administration of chlorpyrifos (CPF), cypermethrin (CYP) and their combination in adult male albino rats. Forty mature male albino rats were separated into four groups (10 each), the first group was used as control, while second, third and fourth groups received orally 1/20 LD50 of CPF (10 mg/kg b.wt), 1/20 LD50 of CYP (17.22 mg/kg b.wt) and 1/40 LD50 of CPF plus 1/40 LD50 of CYP (5 mg/kg b.wt CPF plus 8.61 mg/kg b.wt CYP) respectively for 26 days. The results revealed that exposure to CPF and/or CYP induced a significant decrease in the reproductive organs weight. Moreover, a significant decrease in spermatic picture (sperm cell concentration and viability) was observed with high percent of sperm abnormalities. Serum levels of testosterone and pituitary gonadotropins (FSH and LH) have been declined significantly in all treated groups. Significant elevations were observed in malondialdehyde and nitric oxide concentrations, while antioxidant enzymes superoxide dismutase and glutathione-S-transferase activities were decreased significantly as a result of induced oxidative stress. A significant drop in prostatic acid phosphatase activity was observed. Additionally, the results showed some histopathological alterations in the reproductive organs as well as neurological lesions in brain and pituitary glands. In conclusion, CPF and CYP induce deleterious effects on reproductive efficiency of male rats which reflect more obvious impacts when both combined

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Vulnerability factors for mephedrone-induced conditioned place preference in rats—the impact of sex differences, social-conditioning and stress

Psychopharmacology ◽

10.1007/s00213-021-05910-y ◽

2021 ◽

Author(s):

Olga Wronikowska ◽

Maria Zykubek ◽

Łukasz Kurach ◽

Agnieszka Michalak ◽

Anna Boguszewska-Czubara ◽

...

Keyword(s):

Sex Differences ◽

Conditioned Place Preference ◽

Low Dose ◽

Social Factor ◽

Place Preference ◽

Female Rats ◽

Male Rats ◽

Social Conditioning ◽

Male And Female Rats ◽

The Impact

Abstract Rationale Mephedrone is a frequently overused drug of abuse that belongs to the group of novel psychoactive substances. Although its mechanism of action, as well as toxic and psychoactive effects, has been widely studied, the role of different factors that could contribute to the increased vulnerability to mephedrone abuse is still poorly understood. Objectives The aim of the presented study was to assess the impact of several factors (sex differences, social-conditioning, and chronic mild unpredictable stress — CMUS) on the liability to mephedrone-induced reward in Wistar rats. Methods The rewarding effects of mephedrone in male and female rats were assessed using the conditioned place preference (CPP) procedure. Furthermore, the impact of social factor and stress was evaluated in male rats using social-CPP and CMUS-dependent CPP, respectively. Results Mephedrone induced classic-CPP in female (10 mg/kg), as well as in male (10 and 20 mg/kg) rats. However, the impact of mephedrone treatment during social-CPP was highly dose-dependent as the rewarding effects of low dose of mephedrone (5 mg/kg; non-active in classic-CPP) were potentiated when administered during social-conditioning. Interestingly, social-conditioning with a higher dose of 20 mg/kg (that induced classic-CPP) was able to reverse these effects. Finally, CMUS potentiated rewarding effects of a low dose of mephedrone (5 mg/kg) and increased the level of corticosterone in rats’ prefrontal cortex and hippocampus. Conclusions Altogether, the presented results give new insight into possible factors underlying the vulnerability to mephedrone abuse and can serve as a basis for further studies assessing mechanisms underlying observed effects.

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Long-term exposure of male rats to low-dose ethinylestradiol (EE2) in drinking water: Effects on ponderal growth and on litter size of their progeny

Reproductive Toxicology ◽

10.1016/j.reprotox.2007.12.002 ◽

2008 ◽

Vol 25 (2) ◽

pp. 161-168 ◽

Cited By ~ 18

Author(s):

Mélanie Vosges ◽

Jean-Claude Braguer ◽

Yves Combarnous

Keyword(s):

Drinking Water ◽

Litter Size ◽

Low Dose ◽

Male Rats ◽

Water Effects

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Adolescent male rats exposed to social defeat exhibit altered anxiety behavior and limbic monoamines as adults.

Behavioral Neuroscience ◽

10.1037/a0015752 ◽

2009 ◽

Vol 123 (3) ◽

pp. 564-576 ◽

Cited By ~ 124

Author(s):

Michael J. Watt ◽

Andrew R. Burke ◽

Kenneth J. Renner ◽

Gina L. Forster

Keyword(s):

Social Defeat ◽

Male Rats ◽

Adolescent Male ◽

Anxiety Behavior

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Neurobehavioral effects of chronic low-dose vanadium administration in young male rats

Behavioural Brain Research ◽

10.1016/j.bbr.2021.113701 ◽

2021 ◽

pp. 113701

Author(s):

Amanda Dyer ◽

Maxine De Butte

Keyword(s):

Low Dose ◽

Young Male ◽

Male Rats ◽

Neurobehavioral Effects

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Effect of gallic acid on the reproduction of adolescent male Brandt’s vole (Lasiopodomys brandtii)

Canadian Journal of Zoology ◽

10.1139/cjz-2020-0293 ◽

2021 ◽

Author(s):

Xin Dai ◽

Xiao-Feng Sun ◽

Ai-Qin Wang ◽

Wanhong Wei ◽

Sheng-Mei Yang

Keyword(s):

Gallic Acid ◽

Low Dose ◽

Regulatory Protein ◽

Antioxidant Properties ◽

Adolescent Male ◽

Seminiferous Tubules ◽

High Dose ◽

Mrna Levels ◽

Dependent Manner ◽

Lasiopodomys Brandtii

Gallic acid (GA), a phenol that is present in various plants, potentially contains antioxidant properties. This study aimed to investigate the effects of GA on the reproduction of adolescent male Brandt’s voles (Lasiopodomys brandtii (Radde, 1861)). Antioxidant levels and apoptosis in the testis, as well as reproductive physiology, were evaluated in adolescent males treated with GA. The results showed that a low dose of GA enhanced relative epididymis weight and the sperm density in the epididymis, increased the mRNA levels of steroidogenic acute regulatory protein in the testis, and reduced the percentages of abnormal and dead sperm. In addition, a low dose of GA significantly increased the levels of superoxide dismutase, catalase, and glutathione peroxidase, and decreased the level of malondialdehyde in the testis, as well as the mRNA and protein levels of the apoptosis related gene, caspase-3. However, a high dose of GA sharply reduced the average diameter of the seminiferous tubules compared to a low dose. Collectively, these findings demonstrate that GA treatment during puberty affects the reproductive responses of male Brandt’s voles in a dose-dependent manner by regulating antioxidant levels and apoptosis.

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