Ultrastructural Techniques to Investigate Cell Wall Degradation and Antiquality Factors in Two Bermudagrass Cultivars

Improved methods are needed to extend the shelf life of strawberry fruits. The objective of this study was to determine the postharvest physiological mechanism of high-CO2 treatment in strawberries. Harvested strawberries were stored at 10 °C after 3 h of exposure to a treatment with 30% CO2 or air. Pectin and gene expression levels related to cell wall degradation were measured to assess the high-CO2 effects on the cell wall and lipid metabolism. Strawberries subjected to high-CO2 treatment presented higher pectin content and firmness and lower decay than those of control fruits. Genes encoding cell wall-degrading enzymes (pectin methylesterase, polygalacturonase, and pectate lyase) were downregulated after high-CO2 treatment. High-CO2 induced the expression of oligogalacturonides, thereby conferring defense against Botrytis cinerea in strawberry fruits, and lowering the decay incidence at seven days after its inoculation. Our findings suggest that high-CO2 treatment can maintain strawberry quality by reducing decay and cell wall degradation.

Download Full-text

A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening

Horticultural Plant Journal ◽

10.1016/j.hpj.2017.08.003 ◽

2017 ◽

Vol 3 (5) ◽

pp. 190-198 ◽

Cited By ~ 2

Author(s):

Wei WEI ◽

Zhongqi FAN ◽

Jianye CHEN ◽

Jianfei KUANG ◽

Wangjin LU ◽

...

Keyword(s):

Cell Wall ◽

Transcription Factor ◽

Fruit Ripening ◽

Cell Wall Degradation ◽

A Cell

Download Full-text

Synergistic effect of gum Arabic and carboxymethyl cellulose as biocomposite coating delays senescence in stored tomatoes by regulating antioxidants and cell wall degradation

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2022.01.073 ◽

2022 ◽

Author(s):

Muhammad Saleem Shakir ◽

Shaghef Ejaz ◽

Sajjad Hussain ◽

Sajid Ali ◽

Hasan Sardar ◽

...

Keyword(s):

Cell Wall ◽

Synergistic Effect ◽

Carboxymethyl Cellulose ◽

Gum Arabic ◽

Cell Wall Degradation

Download Full-text

Effects of Drying and Ensiling on In situ Cell Wall Degradation Kinetics of Tomato Pomace in Ruminant

Asian Journal of Animal Sciences ◽

10.3923/ajas.2012.196.202 ◽

2012 ◽

Vol 6 (4) ◽

pp. 196-202 ◽

Cited By ~ 1

Author(s):

Naser Maheri-Sis ◽

Mohammad Chamani ◽

Ali Asghar Sadeghi ◽

Ali Mirzaaghaz ◽

Kambiz Nazeradl ◽

...

Keyword(s):

Cell Wall ◽

Degradation Kinetics ◽

Cell Wall Degradation ◽

Tomato Pomace ◽

Kinetics Of

Download Full-text

Random Mutagenesis of Clostridium cellulolyticum by Using a Tn1545 Derivative

Applied and Environmental Microbiology ◽

10.1128/aem.02417-09 ◽

2010 ◽

Vol 76 (13) ◽

pp. 4546-4549 ◽

Cited By ~ 9

Author(s):

Jean-Charles Blouzard ◽

Odile Valette ◽

Chantal Tardif ◽

Pascale de Philip

Keyword(s):

Cell Wall ◽

Metabolic Engineering ◽

Plant Cell ◽

Plant Cell Wall ◽

Random Mutagenesis ◽

Cell Wall Degradation ◽

Clostridium Cellulolyticum ◽

Plant Cell Wall Degradation ◽

Random Insertion

ABSTRACT Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.

Download Full-text

Aminoethoxyvinylglycine Inhibits Fruit Abscission Induced by Naphthaleneacetic Acid and Associated Relationships with Expression of Genes for Ethylene Biosynthesis, Perception, and Cell Wall Degradation in ‘Delicious’ Apples

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.133.6.727 ◽

2008 ◽

Vol 133 (6) ◽

pp. 727-734 ◽

Cited By ~ 7

Author(s):

Hong Zhu ◽

Eric P. Beers ◽

Rongcai Yuan

Keyword(s):

Cell Wall ◽

Ethylene Production ◽

Acc Oxidase ◽

Ethylene Biosynthesis ◽

Naphthaleneacetic Acid ◽

Cell Wall Degradation ◽

Oxidase Gene ◽

Young Fruit ◽

Fruit Abscission ◽

Expression Of Genes

Effects of naphthaleneacetic acid (NAA) and aminoethoxyvinylglycine (AVG) on young fruit abscission, leaf and fruit ethylene production, and expression of genes related to ethylene biosynthesis and cell wall degradation were examined in ‘Delicious’ apples (Malus ×domestica Borkh.). NAA at 15 mg·L−1 increased fruit abscission and ethylene production of leaves and fruit when applied at the 11-mm stage of fruit development, whereas AVG, an inhibitor of ethylene biosynthesis, at 250 mg·L−1 reduced NAA-induced fruit abscission and ethylene production of leaves and fruit. NAA also increased expression of 1-aminocyclopropane-1-carboxylate (ACC) synthase genes (MdACS5A and MdACS5B), ACC oxidase gene (MdACO1), and ethylene receptor genes (MdETR1a, MdETR1b, MdETR2, MdERS1, and MdERS2) in fruit cortex and fruit abscission zones. However, AVG reduced NAA-induced expression of these genes except for MdERS2 in fruit abscission zones. NAA increased expression of the polygalacturonase gene MdPG2 in fruit abscission zones but not in fruit cortex, whereas AVG reduced NAA-enhanced expression of MdPG2 in fruit abscission zones. The expression of β-1,4-glucanase gene MdCel1 in fruit abscission zones was decreased by NAA but was unaffected by AVG. Our results suggest that ethylene biosynthesis, ethylene perception, and the MdPG2 gene are involved in young fruit abscission caused by NAA.

Download Full-text

Feruloyl and p-coumaroyl esterase from anaerobic fungi in relation to plant cell wall degradation

Applied Microbiology and Biotechnology ◽

10.1007/bf00164534 ◽

1990 ◽

Vol 33 (3) ◽

pp. 345-351 ◽

Cited By ~ 127

Author(s):

William S. Borneman ◽

Roy D. Hartley ◽

W. Herbert Morrison ◽

Danny E. Akin ◽

Lars G. Ljungdahl

Keyword(s):

Cell Wall ◽

Plant Cell ◽

Plant Cell Wall ◽

Anaerobic Fungi ◽

Cell Wall Degradation ◽

Plant Cell Wall Degradation

Download Full-text

Requirement of Autolytic Activity for Bacteriocin-Induced Lysis

Applied and Environmental Microbiology ◽

10.1128/aem.66.8.3174-3179.2000 ◽

2000 ◽

Vol 66 (8) ◽

pp. 3174-3179 ◽

Cited By ~ 45

Author(s):

M. Carmen Martínez-Cuesta ◽

Jan Kok ◽

Elisabet Herranz ◽

Carmen Peláez ◽

Teresa Requena ◽

...

Keyword(s):

Cell Wall ◽

Sodium Dodecyl Sulfate ◽

Growth Phase ◽

Cell Walls ◽

Cell Damage ◽

Sodium Dodecyl ◽

Exponential Growth Phase ◽

Cell Wall Degradation ◽

Intracellular Proteins ◽

Autolytic Activity

ABSTRACT The bacteriocin produced by Lactococcus lactis IFPL105 is bactericidal against several Lactococcus andLactobacillus strains. Addition of the bacteriocin to exponential-growth-phase cells resulted in all cases in bacteriolysis. The bacteriolytic response of the strains was not related to differences in sensitivity to the bacteriocin and was strongly reduced in the presence of autolysin inhibitors (Co2+ and sodium dodecyl sulfate). When L. lactis MG1363 and its derivative deficient in the production of the major autolysin AcmA (MG1363acmAΔ1) were incubated with the bacteriocin, the latter did not lyse and no intracellular proteins were released into the medium. Incubation of cell wall fragments of L. lactisMG1363, or of L. lactis MG1363acmAΔ1 to which extracellular AcmA was added, in the presence or absence of the bacteriocin had no effect on the speed of cell wall degradation. This result indicates that the bacteriocin does not degrade cell walls, nor does it directly activate the autolysin AcmA. The autolysin was also responsible for the observed lysis of L. lactis MG1363 cells during incubation with nisin or the mixture of lactococcins A, B, and M. The results presented here show that lysis of L. lactis after addition of the bacteriocins is caused by the resulting cell damage, which promotes uncontrolled degradation of the cell walls by AcmA.

Download Full-text