Synergistic effect of gum Arabic and carboxymethyl cellulose as biocomposite coating delays senescence in stored tomatoes by regulating antioxidants and cell wall degradation

2022 ◽  
Author(s):  
Muhammad Saleem Shakir ◽  
Shaghef Ejaz ◽  
Sajjad Hussain ◽  
Sajid Ali ◽  
Hasan Sardar ◽  
...  
Keyword(s):  
Cell Wall ◽  
Synergistic Effect ◽  
Carboxymethyl Cellulose ◽  
Gum Arabic ◽  
Cell Wall Degradation

scholarly journals Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation

2014 ◽  
Vol 111 (17) ◽  
pp. 6287-6292 ◽  
Author(s):  
J. W. Agger ◽  
T. Isaksen ◽  
A. Varnai ◽  
S. Vidal-Melgosa ◽  
W. G. T. Willats ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Degradation ◽  
Oxidative Processes ◽  
Plant Cell Wall Degradation

scholarly journals High-CO2 Treatment Prolongs the Postharvest Shelf Life of Strawberry Fruits by Reducing Decay and Cell Wall Degradation

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1649
Author(s):  
Hyang-Lan Eum ◽  
Seung-Hyun Han ◽  
Eun-Jin Lee
Keyword(s):  
Cell Wall ◽  
Shelf Life ◽  
Physiological Mechanism ◽  
Pectate Lyase ◽  
Pectin Methylesterase ◽  
Cell Wall Degradation ◽  
Cell Wall Degrading Enzymes ◽  
High Co2 ◽  
Genes Encoding ◽  
Co2 Treatment

Improved methods are needed to extend the shelf life of strawberry fruits. The objective of this study was to determine the postharvest physiological mechanism of high-CO2 treatment in strawberries. Harvested strawberries were stored at 10 °C after 3 h of exposure to a treatment with 30% CO2 or air. Pectin and gene expression levels related to cell wall degradation were measured to assess the high-CO2 effects on the cell wall and lipid metabolism. Strawberries subjected to high-CO2 treatment presented higher pectin content and firmness and lower decay than those of control fruits. Genes encoding cell wall-degrading enzymes (pectin methylesterase, polygalacturonase, and pectate lyase) were downregulated after high-CO2 treatment. High-CO2 induced the expression of oligogalacturonides, thereby conferring defense against Botrytis cinerea in strawberry fruits, and lowering the decay incidence at seven days after its inoculation. Our findings suggest that high-CO2 treatment can maintain strawberry quality by reducing decay and cell wall degradation.

scholarly journals A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening

2017 ◽  
Vol 3 (5) ◽  
pp. 190-198 ◽  
Author(s):  
Wei WEI ◽  
Zhongqi FAN ◽  
Jianye CHEN ◽  
Jianfei KUANG ◽  
Wangjin LU ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Fruit Ripening ◽  
Cell Wall Degradation ◽  
A Cell

Effects of Drying and Ensiling on In situ Cell Wall Degradation Kinetics of Tomato Pomace in Ruminant

2012 ◽  
Vol 6 (4) ◽  
pp. 196-202 ◽  
Author(s):  
Naser Maheri-Sis ◽  
Mohammad Chamani ◽  
Ali Asghar Sadeghi ◽  
Ali Mirzaaghaz ◽  
Kambiz Nazeradl ◽  
...  
Keyword(s):  
Cell Wall ◽  
Degradation Kinetics ◽  
Cell Wall Degradation ◽  
Tomato Pomace ◽  
Kinetics Of

scholarly journals Random Mutagenesis of Clostridium cellulolyticum by Using a Tn1545 Derivative

2010 ◽  
Vol 76 (13) ◽  
pp. 4546-4549 ◽  
Author(s):  
Jean-Charles Blouzard ◽  
Odile Valette ◽  
Chantal Tardif ◽  
Pascale de Philip
Keyword(s):  
Cell Wall ◽  
Metabolic Engineering ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Random Mutagenesis ◽  
Cell Wall Degradation ◽  
Clostridium Cellulolyticum ◽  
Plant Cell Wall Degradation ◽  
Random Insertion

ABSTRACT Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.

scholarly journals Golgi-localized exo-β1,3-galactosidases involved in AGP modification and root cell expansion in Arabidopsis

2020 ◽  
Author(s):  
Pieter Nibbering ◽  
Bent L. Petersen ◽  
Mohammed Saddik Motawia ◽  
Bodil Jørgensen ◽  
Peter Ulvskov ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Expansion ◽  
Glycosyl Hydrolase ◽  
Functional Characterization ◽  
Gum Arabic ◽  
Root Cell ◽  
Growth Media ◽  
Galactosidase Activity ◽  
Loss Of Function ◽  
Yariv Phenylglycoside

AbstractPlant arabinogalactan proteins (AGPs) are a diverse group of cell surface- and wall-associated glycoproteins. Functionally important AGP glycans are synthesized in the Golgi apparatus, but the relationships between their glycosylation, processing, and functionality are poorly understood. Here we report the identification and functional characterization of two Golgi-localized exo-β-1,3-galactosidases from the glycosyl hydrolase 43 (GH43) family in Arabidopsis thaliana. GH43 loss of function mutants exhibit root cell expansion defects in sugar-containing growth media. This root phenotype is associated with an increase in the extent of AGP cell wall association, as demonstrated by Yariv phenylglycoside dye quantification and comprehensive microarray polymer profiling of sequentially extracted cell walls. Recombinant GH43 characterization showed that the exo-β-1,3-galactosidase activity of GH43s is hindered by β-1,6 branches on β-1,3-galactans. In line with this steric hindrance, the recombinant GH43s did not release galactose from cell wall extracted glycoproteins or AGP rich gum arabic. These results show that Arabidopsis GH43s are involved in AGP glycan biosynthesis in the Golgi, and suggest their exo-β-1,3-galactosidase activity influences AGP and cell wall matrix interactions, thereby adjusting cell wall extensibility.

scholarly journals Aminoethoxyvinylglycine Inhibits Fruit Abscission Induced by Naphthaleneacetic Acid and Associated Relationships with Expression of Genes for Ethylene Biosynthesis, Perception, and Cell Wall Degradation in ‘Delicious’ Apples

2008 ◽  
Vol 133 (6) ◽  
pp. 727-734 ◽  
Author(s):  
Hong Zhu ◽  
Eric P. Beers ◽  
Rongcai Yuan
Keyword(s):  
Cell Wall ◽  
Ethylene Production ◽  
Acc Oxidase ◽  
Ethylene Biosynthesis ◽  
Naphthaleneacetic Acid ◽  
Cell Wall Degradation ◽  
Oxidase Gene ◽  
Young Fruit ◽  
Fruit Abscission ◽  
Expression Of Genes

Effects of naphthaleneacetic acid (NAA) and aminoethoxyvinylglycine (AVG) on young fruit abscission, leaf and fruit ethylene production, and expression of genes related to ethylene biosynthesis and cell wall degradation were examined in ‘Delicious’ apples (Malus ×domestica Borkh.). NAA at 15 mg·L−1 increased fruit abscission and ethylene production of leaves and fruit when applied at the 11-mm stage of fruit development, whereas AVG, an inhibitor of ethylene biosynthesis, at 250 mg·L−1 reduced NAA-induced fruit abscission and ethylene production of leaves and fruit. NAA also increased expression of 1-aminocyclopropane-1-carboxylate (ACC) synthase genes (MdACS5A and MdACS5B), ACC oxidase gene (MdACO1), and ethylene receptor genes (MdETR1a, MdETR1b, MdETR2, MdERS1, and MdERS2) in fruit cortex and fruit abscission zones. However, AVG reduced NAA-induced expression of these genes except for MdERS2 in fruit abscission zones. NAA increased expression of the polygalacturonase gene MdPG2 in fruit abscission zones but not in fruit cortex, whereas AVG reduced NAA-enhanced expression of MdPG2 in fruit abscission zones. The expression of β-1,4-glucanase gene MdCel1 in fruit abscission zones was decreased by NAA but was unaffected by AVG. Our results suggest that ethylene biosynthesis, ethylene perception, and the MdPG2 gene are involved in young fruit abscission caused by NAA.

scholarly journals The impact of enological products for tartaric stabilization on wine filterability

2021 ◽  
pp. 3-3
Author(s):  
Vladimir Puskas ◽  
Uros Miljic ◽  
Vesna Vucurovic
Keyword(s):  
Carboxymethyl Cellulose ◽  
Red Wine ◽  
Gum Arabic ◽  
White Rose ◽  
The Impact ◽  
Rosé Wine

The aim of this research was to evaluate the impact of most commonly used agents for tartaric stabilization of wine, such as metatartaric acid, carboxymethyl cellulose (CMC), mannoproteins and gum arabic, on the filterability of white, rose and red wine previously pre-filtered and prepared for the final filtration and bottling. Wine filterability after sweetening with rectified concentrated must was also assessed. The experiments were carried out using Quality Filterability Test and the obtained results were expressed through filterability index (FI), and maximum filterable volume (Vmax). The results confirmed that used enological agents for the inhibition of tartaric instabilities generally did not worsen the filterability of white and rose wine (FI<20). However, it was evident that products containing mannoproteins and metatartaric acid considerably reduced red wine filterability (FI>500). The correlation between white and rose wine turbidity and filterability was recorded in the trials but the same trend was not registered for the red wine. The results of this study are important since membrane (final) filtration of improperly prepared wine characterized by low filterability can both increase the costs and lead to hold up on the bottling line.

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