Performance of Silica-Gel as a Geochemical Barrier Under CO2 Storage Conditions

2019 ◽  
Author(s):  
Jay R. Black ◽  
César Castañeda-Herrera ◽  
Ella Llanos ◽  
Geoff Stevens ◽  
Ralf Haese
2018 ◽  
Vol 78 ◽  
pp. 27-36 ◽  
Author(s):  
C.A. Castañeda-Herrera ◽  
J.R. Black ◽  
E.M. Llanos ◽  
G.W. Stevens ◽  
R.R. Haese

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Michael J. Allison ◽  
Jessica M. Round ◽  
Lauren C. Bergman ◽  
Ali Mirabzadeh ◽  
Heather Allen ◽  
...  

Abstract Objective Silica gel beads have promise as a non-toxic, cost-effective, portable method for storing environmental DNA (eDNA) immobilized on filter membranes. Consequently, many ecological surveys are turning to silica bead filter desiccation rather than ethanol preservation. However, no systematic evaluation of silica bead storage conditions or duration past 1 week has been published. The present study evaluates the quality of filter-immobilized eDNA desiccated with silica gel under different storage conditions for over a year using targeted quantitative real-time polymerase chain reaction (qPCR)-based assays. Results While the detection of relatively abundant eDNA target was stable over 15 months from either ethanol- or silica gel-preserved filters at − 20 and 4 °C, silica gel out-performed ethanol preservation at 23 °C by preventing a progressive decrease in eDNA sample quality. Silica gel filter desiccation preserved low abundance eDNA equally well up to 1 month regardless of storage temperature (18, 4, or − 20 °C). However only storage at − 20 °C prevented a noticeable decrease in detectability at 5 and 12 months. The results indicate that brief storage of eDNA filters with silica gel beads up to 1 month can be successfully accomplished at a range of temperatures. However, longer-term storage should be at − 20 °C to maximize sample integrity.


2015 ◽  
Vol 399 ◽  
pp. 134-144 ◽  
Author(s):  
Jay R. Black ◽  
Susan A. Carroll ◽  
Ralf R. Haese

Author(s):  
L.J. Wasch ◽  
M. Koenen ◽  
J. Wollenweber ◽  
J.H. ter Heege ◽  
T.J. Tambach

2016 ◽  
Vol 72 ◽  
pp. 59-76 ◽  
Author(s):  
Julie K. Pearce ◽  
Grant K.W. Dawson ◽  
Alison C.K. Law ◽  
Dean Biddle ◽  
Suzanne D. Golding

2013 ◽  
Vol 13 (2) ◽  
pp. 151 ◽  
Author(s):  
Alejandro Jaramillo ◽  
Mauricio Martínez ◽  
Carolina Cardozo ◽  
Jesús Burgos

<p>Uno de los inconvenientes en la producción agrícola es el suministro adecuado y oportuno de semillas. La ausencia de métodos de almacenamiento controlado de semillas de cítricos en los viveros se convierte en la principal limitante para la producción de material de siembra, pues cerca del 40% de este material se pierde por mala calidad fisiológica de las semillas. El objetivo del trabajo fue determinar las condiciones para almacenar semillas de Citrumelo CPB 4475 [<em>Citrus paradisi </em>Macfad. × <em>Poncirus trifoliate </em>(L.) Raf.], Sunki × English [<em>Citrus sunki </em>Hort. ex Tan. × <em>Poncirustri foliata </em>(L.) Raf.] y limón ‘Volkameriana’ (<em>Citrus volkameriana </em>Ten. y Pasq.), comúnmente utilizados como portainjertos de lima ácida ‘Tahití’ (<em>Citrus latifolia </em>Tanaka). Las semillas fueron empacadas en bolsas y frascos de polietileno con tres niveles de humedad en base fresco del 10% a 9%, 8% a 7% y 6% a 5%, bajo dos condiciones de almacenamiento (10°C, 70 % HR y 24°C, 80% HR), durante 30, 90 y 180 días. El efecto de la desecación, el tiempo y el empaque de almacenamiento se midió en porcentaje de germinación. Las semillas de los tres portainjertos alcanzaron un nivel de humedad entre el 10% y 9% después de 3 días de secado a temperatura ambiente y niveles del 8% a 7% y 6% a 5% después de 2 y 14 horas de secado en sílica gel. Los mayores valores de germinación estuvieron entre los 0 y 30 días. El mayor porcentaje de germinación se presentó con el nivel de humedad entre el 10% y 9%, para todas las semillas de los cítricos evaluados. El experimento mostró que para las semillas de los tres materiales estudiados hubo mayor germinación a una temperatura promedio de 10°C con un 70% de HR. El tipo de empaque no presentó diferencias significativas. </p><p> </p><p><strong>Determination of safe storage under controlled for rootstock seed of ‘Tahiti’ limes </strong></p><p>One of the disadvantages in agricultural production is the adequate and timely supply of seeds. The absence of controlled storage methods of citrus seeds in nurseries is the main limiting factor for the production of planting material, because 40% of this material is lost through poor physiological quality of the seeds. The objective of this study was to determine the best conditions for storing seeds of CPB Citrumelo 4475 [<em>Citrus paradise </em>Macfad. × <em>Poncirustrifoliata </em>(L.) Raf.], Sunki × English [<em>Citrus sunki </em>Hort. ex Tan × <em>Poncirus trifoliata </em>(L.)Raf.] and ‘Volkameriana’ lemon (<em>C. volkameriana </em>Ten. and Pasq.), commonly used as rootstocks for ‘Tahiti’ limes (<em>C. latifolia </em>Tanaka). The seeds were packed in polyethylene bags and flasks with three levels of moisture on a fresh base: 10%-9%, 8%-7% and 6%-5%, under two storage conditions (10°C, 70% RH and 24°C, 80% RH) for 30, 90 and 180 days. The viability of the seeds was determined based on seed germination. Seeds from all evaluated plants reached a moisture level of 10%-9% after 3 days of drying at room temperature and levels of 8% to 7% and 6% to 5% after 2 hours and 14 hours when dried with silica gel. The highest germination values were between 0 and 30 days. The highest percentage of germination occurred with a moisture level of 10% and 9% for all tested citrus seeds. The experiment showed that the seeds from the three rootstocks had higher germination at an average temperature of 10°C with 70% RH. The type of packaging did not show significant differences. </p>


2021 ◽  
Vol 50 (8) ◽  
pp. 2229-2240
Author(s):  
Abdullah Al Mamun ◽  
Payap Masniyom ◽  
Jaruwan Maneesri

Low-temperature drying was applied as simple and low cost drying technique for the production of dried Lactobacillus plantarum TISTR 2083, which was isolated from traditional starter of Ka-nom Tuay-fu. To improve the survival of L. plantarum TISTR 2083 during drying and storage, five different protectants and two carrier materials were investigated. These include sucrose, trehalose, maltodextrin, skim milk, and L-glutamate sodium salt (L-GSS) as protectant and rice starch and rice flour as carrier material. Whereas, skim milk as protectant with rice starch resulted in higher viable cell (8.71 log CFU/g) after drying by hot air oven at 40 °C. Different concentration 5, 10, and 15% (w/v) skim milk also investigated to check the effect of skim milk concentration on cell viability. L. plantarum TISTR 2083 starter powder was in different storage conditions to check the storage stability. After 90 days of storage, starter powder stored at 4 °C with silica gel and without silica gel had more than 80% survival rate, while there was no viable cell that stored in room temperature with silica gel. The result showed the production and storage conditions of high viability L. plantarum TISTR 2083, which can be used as starter culture for further fermented product development and as probiotic.


2009 ◽  
Vol 1 (1) ◽  
pp. 3603-3607 ◽  
Author(s):  
Brian Strazisar ◽  
Barbara Kutchko ◽  
Nicolas Huerta

2019 ◽  
pp. 1335-1342
Author(s):  
Stefânia Vilas Boas Coelho ◽  
Sttela Dellyzete Veiga Franco da Rosa ◽  
Tatiana Botelho Fantazzini ◽  
Luciano Coutinho Silva

Seeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzymes catalase, esterase, glutamic oxaloacetic transaminase, and polyphenol oxidase are indicators of seed quality for Coffea canephora seeds subjected to cryopreservation.


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