scholarly journals Cryopreservation of Coffea canephora Pierre ex A. Froehner seeds: importance of drying rate and moisture content

2019 ◽  
pp. 1335-1342
Author(s):  
Stefânia Vilas Boas Coelho ◽  
Sttela Dellyzete Veiga Franco da Rosa ◽  
Tatiana Botelho Fantazzini ◽  
Luciano Coutinho Silva

Seeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzymes catalase, esterase, glutamic oxaloacetic transaminase, and polyphenol oxidase are indicators of seed quality for Coffea canephora seeds subjected to cryopreservation.

2016 ◽  
Vol 38 (3) ◽  
pp. 241-247 ◽  
Author(s):  
Heloisa Oliveira dos Santos ◽  
Maria Laene Moreira de Carvalho ◽  
Carla Massimo Caldeira ◽  
Stefânia Vilas Boas Coelho ◽  
Edila Vilela de Resende Von Pinho ◽  
...  

Abstract: The present study aimed to evaluate effects of different storage conditions on the castor bean seed cultivar IAC-226 quality, stored for 12 months. For this purpose, seeds were stored in different environment and packaging conditions: in a cold chamber and conventional storage, using multiwall Kraft paper and in no vacuum and vacuum plastic packages at 1 atm; as well as under cryopreservation storage (-196 °C). Seed quality was evaluated before and after 4, 8 and 12 months of storage by germination tests, first count of germination, emergence percentage, emergence speed index and determination of changes in catalase (CAT) and superoxide dismutase (SOD) enzyme systems. Cryopreservation (-196 °C) is efficient in maintaining the physiological quality of castor bean cultivar IAC-226 for 12 months. The enzyme catalase stands out as a marker of castor seed deterioration during storage.


2005 ◽  
Vol 17 (2) ◽  
pp. 199-205 ◽  
Author(s):  
Sttela Dellyzete Veiga Franco da Rosa ◽  
Delacyr da Silva Brandão Júnior ◽  
Édila Vilela de Resende Von Pinho ◽  
André Delly Veiga ◽  
Luiz Hildebrando de Castro e Silva

Desiccation tolerance in seeds depends on the species, development stage and drying conditions, especially the water removal rate. Coffea seeds are considered of intermediate performance, because they tolerate relative dehydration compared to orthodox seeds and are sensitive to low temperatures. The objective of this study was to verify the effect of different drying rates on the viability and storability of Coffea canephora seeds. A complete randomized experimental design was used, in a factorial 3 x 5 x 2 design, with three drying rates (fast, intermediate and slow), five final mean water contents after drying (51, 42, 33, 22 and 15 %) and two storage temperatures (10 and 20°C). The germination and seed vigor assessments, using radicle protrusion, cotyledon leaf opening, seedling emergence and emergence speed index, were performed shortly after drying and after two and four months storage. It was observed that with reduction in the water content there was reduction in the germination values and seed vigor, for all the drying rates. The greatest reductions in physiological quality occurred when the seeds were dried quickly and the best results were obtained at the intermediate drying rate. There was an effect of drying rate and storage temperature on the physiological quality of the seeds, and lower germination and vigor values were observed in seeds with lower water content stored at 20°C. C. canephora seeds were tolerant to desiccation down to 15 % water content and can be stored for four months at 10°C. A temperature of 20ºC can be used to store C. canephora seeds, as long as the water content is not reduced to values below 22 % water content.


2017 ◽  
Vol 38 (1) ◽  
pp. 57
Author(s):  
Daniele Piano Rosa ◽  
Danúbia Aparecida Costa Nobre ◽  
Diego Santos Oliveira ◽  
Francisco Charles dos Santos Silva ◽  
André Ricardo Gomes Bezerra ◽  
...  

This study aimed to assess the effect of genetic diversity on physiological quality of soybean seeds stored in cold chamber and under environmental conditions. Ten cultivars were assessed in a randomized factorial design (2x10). Factor 1 corresponded to two storage conditions and factor 2 to ten soybean cultivars, with four replications. The evaluated variables were total germination (G%), first count of germination (F%), percentage of abnormal seedlings (AS%), germination speed index (GSI), water content (WC), electrical conductivity (EC), dry matter of seedlings (DMS) and length of seedlings (LS). Data underwent ANOVA, followed by Scott Knott test, as well as multivariate analysis of genetic diversity. The results showed a higher physiological quality for seeds under cold storage. Half of the cultivars (FPS Júpiter, FPS Urano, FPS Antares, FPS Netuno and CD 250) presented high germination rates and seed vigor, being thus indicated as high-standard materials for further breeding programs. Besides that, storage environment had influence on the clustering of soybean cultivars. Moreover, cultivars had genetic dissimilarity for almost all assessed traits as G%, GSI, F%, AS%, EC, DMS and LS.


Author(s):  
Emilly R. Alkimim ◽  
Andréia M. S. de S. David ◽  
Tiago V. Sousa ◽  
Cleiton G. Rodrigues ◽  
Hugo T. R. Amaro

ABSTRACT The season of harvest is one of the most important factors influencing seed quality. This study evaluated the effect of different harvesting times on the physiological quality of Coriandrum sativum L. seeds, cv. Palmeira. The experimental design was completely randomized, with six treatments (harvest times) and four replications. The experiment was conducted during the months of April to October 2010. The first moment to harvest seeds was carried out 15 days after full bloom, when approximately 50% of plants were in flowering phase. Other harvests (22, 29, 36, 43 and, 50 days after bloom) were carried out at 7 days intervals until the seeds reached 16.0% of moisture, which occurred in the 6th harvest. Immediately after each harvest, the seeds were analysed for water content, germination and vigor (first count, seedling emergence and emergence rate index). The harvest season most suitable for C. sativum seeds cv. Palmeira occurred between 46 and 50 days after flowering, when the seeds have the highest physiological quality and moisture content between 20 and 16%.


2018 ◽  
Vol 14 (3) ◽  
pp. 132-143
Author(s):  
Lizandro Ciciliano Tavares ◽  
Sandro de Oliveira ◽  
Bruno Moncks Silva ◽  
Elisa Souza Lemes ◽  
Francisco Amaral Villela ◽  
...  

In the wheat crop,pre-harvest desiccation with herbicides caninfluencethe productivity and physiological seed quality.The study aimed to quantify the yield, physiological seed quality,andresidue in wheat seeds.The experimental design was a randomized block designina 2x2x6 factorial, being thefactor A constituting of wheat cultivars (BRS 177 e BRS Umbu),the factor B application stages (before and after physiological maturation) and, factor C desiccation with different herbicides (2,4-D, paraquat, glyphosate, ammonium glufosinate, metsulfuron-methyl, and control) with four replications.The variables analyzed were thethousand seedsweight, hectoliter weight, number of antheciumper spike, seed yield, physiological seed quality by the vigortestsand standard germination, and residue in wheat seeds after the application of herbicides in pre-harvest.The desiccation in pre-harvest herbicide held at a later stage after physiological maturity, in general, does not affect the physiological quality and seed yield of BRS Umbu and BRS 177 cultivars. The application of methyl-metsulfuronand paraquat does not show residual effects on the seed independent of the application stage, while the application of ammonium glufosinate, glyphosate, and 2,4-D,when applied before the physiological maturity result residue levels in wheat seed.


2018 ◽  
Vol 40 (1) ◽  
pp. 52-59
Author(s):  
Solange Carvalho Barrios Roveri Jose ◽  
Antonieta Nassif Salomão ◽  
Luis Alberto Martins Palhares de Melo ◽  
Izulmé Rita Imaculada Santos ◽  
Bruno Galvêas Laviola

Abstract: Jatropha seeds are classified as orthodox. However, since it is an oil seed species, adequate storage conditions are required to ensure their longevity. The objective of this work was to evaluate the physiological quality of jatropha seeds stored in different environments and packaging, for periods of 3, 9 and 15 months. Three types of seed packaging bags (high density plastic bag, aluminized envelope and multiwall paper bag) were used, and the storage environments were cold and dry chamber (20 °C and 15% RH, constant), refrigerator (7 ± 3 °C, 48 ± 8% RH) and laboratory conditions (25 ± 3 °C, 51 ± 7% RH). The initial moisture content and seed germination were 7.1% and 89%, respectively. During storage, the physiological quality (germination and vigor) and moisture content of the seeds were evaluated. Seed water content ranged from 3.3 to 7.7%, depending on the permeability of the packaging and the storage environment. The highest longevity (15 months) without loss of viability was observed for jatropha seeds with initial moisture of 7.1%, packed in semipermeable plastic. Seed vigor was maintained, regardless of the environment and the type of packaging used, for up to nine months of storage.


2013 ◽  
Vol 13 (2) ◽  
pp. 151 ◽  
Author(s):  
Alejandro Jaramillo ◽  
Mauricio Martínez ◽  
Carolina Cardozo ◽  
Jesús Burgos

<p>Uno de los inconvenientes en la producción agrícola es el suministro adecuado y oportuno de semillas. La ausencia de métodos de almacenamiento controlado de semillas de cítricos en los viveros se convierte en la principal limitante para la producción de material de siembra, pues cerca del 40% de este material se pierde por mala calidad fisiológica de las semillas. El objetivo del trabajo fue determinar las condiciones para almacenar semillas de Citrumelo CPB 4475 [<em>Citrus paradisi </em>Macfad. × <em>Poncirus trifoliate </em>(L.) Raf.], Sunki × English [<em>Citrus sunki </em>Hort. ex Tan. × <em>Poncirustri foliata </em>(L.) Raf.] y limón ‘Volkameriana’ (<em>Citrus volkameriana </em>Ten. y Pasq.), comúnmente utilizados como portainjertos de lima ácida ‘Tahití’ (<em>Citrus latifolia </em>Tanaka). Las semillas fueron empacadas en bolsas y frascos de polietileno con tres niveles de humedad en base fresco del 10% a 9%, 8% a 7% y 6% a 5%, bajo dos condiciones de almacenamiento (10°C, 70 % HR y 24°C, 80% HR), durante 30, 90 y 180 días. El efecto de la desecación, el tiempo y el empaque de almacenamiento se midió en porcentaje de germinación. Las semillas de los tres portainjertos alcanzaron un nivel de humedad entre el 10% y 9% después de 3 días de secado a temperatura ambiente y niveles del 8% a 7% y 6% a 5% después de 2 y 14 horas de secado en sílica gel. Los mayores valores de germinación estuvieron entre los 0 y 30 días. El mayor porcentaje de germinación se presentó con el nivel de humedad entre el 10% y 9%, para todas las semillas de los cítricos evaluados. El experimento mostró que para las semillas de los tres materiales estudiados hubo mayor germinación a una temperatura promedio de 10°C con un 70% de HR. El tipo de empaque no presentó diferencias significativas. </p><p> </p><p><strong>Determination of safe storage under controlled for rootstock seed of ‘Tahiti’ limes </strong></p><p>One of the disadvantages in agricultural production is the adequate and timely supply of seeds. The absence of controlled storage methods of citrus seeds in nurseries is the main limiting factor for the production of planting material, because 40% of this material is lost through poor physiological quality of the seeds. The objective of this study was to determine the best conditions for storing seeds of CPB Citrumelo 4475 [<em>Citrus paradise </em>Macfad. × <em>Poncirustrifoliata </em>(L.) Raf.], Sunki × English [<em>Citrus sunki </em>Hort. ex Tan × <em>Poncirus trifoliata </em>(L.)Raf.] and ‘Volkameriana’ lemon (<em>C. volkameriana </em>Ten. and Pasq.), commonly used as rootstocks for ‘Tahiti’ limes (<em>C. latifolia </em>Tanaka). The seeds were packed in polyethylene bags and flasks with three levels of moisture on a fresh base: 10%-9%, 8%-7% and 6%-5%, under two storage conditions (10°C, 70% RH and 24°C, 80% RH) for 30, 90 and 180 days. The viability of the seeds was determined based on seed germination. Seeds from all evaluated plants reached a moisture level of 10%-9% after 3 days of drying at room temperature and levels of 8% to 7% and 6% to 5% after 2 hours and 14 hours when dried with silica gel. The highest germination values were between 0 and 30 days. The highest percentage of germination occurred with a moisture level of 10% and 9% for all tested citrus seeds. The experiment showed that the seeds from the three rootstocks had higher germination at an average temperature of 10°C with 70% RH. The type of packaging did not show significant differences. </p>


ForScience ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. e00663
Author(s):  
Carlos Manoel Oliveira

Os setores de produção e de conservação de sementes dependem diretamente de trabalhos que determinem as melhores condições de armazenamento e que, consequentemente, garantam o maior período possível de manutenção da qualidade fisiológica da semente. Em regiões tropicais, há uma maior limitação no que tange à manutenção da qualidade fisiológica durante o período de armazenamento, principalmente em ambiente não controlado, cuja variação das condições ambientais pode influenciar sobremaneira a qualidade final de um lote de sementes. Objetivou-se, com o presente trabalho, avaliar a qualidade de sementes de duas cultivares de jiló (Solanum gilo Raddi), armazenadas em condições ambientais não controladas. O delineamento experimental utilizado foi o de blocos casualizados com 5 repetições, em esquema fatorial 2x5, sendo duas cultivares de jiló (Morro Grande e Português) e 5 períodos de armazenamento (0, 30, 60, 90 e 120 dias de armazenamento). Avaliou-se a porcentagem de germinação, a porcentagem de emergência, o Índice de Velocidade de Emergência, o Coeficiente de Variação no Tempo, o Tempo Médio da Emergência, os Índices de Incerteza e de Sincronia da Emergência e a Frequência Relativa da Emergência de plântulas. A cultivar Português se apresentou com os melhores índices de porcentagem de germinação, porcentagem de emergência, índice de velocidade de emergência, tempo médio da emergência e coeficiente de variação do tempo da emergência, quando as sementes foram armazenadas por até dias em ambiente não controlado. A cultivar Português é indicada aos produtores de jiló visando produção de mudas, pois se mostrou superior a cultivar Morro Grande quando armazenadas por até 30 dias em ambiente não controlado. Após 30 dias de armazenamento, a utilização de sementes das duas cultivares não é indicado, pois a porcentagem de germinação cai consideravelmente. Palavras-Chave: Fitotecnia. Produção de sementes. Armazenamento.Viabilidade.   Seed quality of scarlet eggplant stored in environmental conditions not controlled Abstract The sectors of production and conservation of seeds depend directly from studies that determine the best storage conditions and that consequently guarantee the longest possible period of maintenance of physiological quality of seed. In tropical regions, there is a major limitation regarding the maintenance of physiological quality during storage period mainly in uncontrolled environment, whose variation of environmental conditions can greatly influence the final quality of a seed lot. The objective of this final paper was to evaluate the quality of seeds of two cultivars of scarlet eggplant (Solanun gilo Raddi), stored in environmental conditions not controlled. The experimental design used was randomized blocks with 05 replications in a factorial 2 x 5, being two cultivars of scarlet eggplant (Morro Grande and Portuguese) and 05 storage periods (0, 30, 60, 90 and 120 days of storage). Was evaluated the Percentage of Germination, Percentage of Emergence, Emergence Speed Index, Coefficient of Variation of the Time, Average Time of Emergency, Indexes of Uncertainty and Sync the Emergence and Relative Frequency of Seedling Emergence in the time. The Portuguese cultivar presented the best rates of Germination Percentage, Emergency Percentage, Emergency Speed Index, Average Emergence Time and Emergence Time Variation Coefficient, when seeds were stored for up to 30 days in an uncontrolled environment. The Portugues cultivar is indicated to jiló producers aiming seedling production because it was superior to Morro Grande cultivar when stored for up to 30 days in an uncontrolled environment. After 30 days of storage, the use of seeds from both cultivars is not indicated because the germination percentage drops considerably.  Keywords: Phytotechny. Seed production. Storage. Viability.


2016 ◽  
Vol 91 (1) ◽  
pp. 40
Author(s):  
Pastora Josefina Querales ◽  
Victor Augusto Forti ◽  
Vanessa Cristina Frare ◽  
Taís Leite Ferreira Pinto ◽  
Annelise Roberta Tremocoldi ◽  
...  

The objective of this study was to verify the health and physiological potential of soybean seeds submitted to different storage conditions. Three batches of soybean seeds were first evaluated for their health and physiological quality with sanitary, germination, seedling emergence in a greenhouse and accelerated aging tests; moisture content was also determined. The seeds were stored for six months under cold chamber (temperature of 10°C and 85% RH), dry chamber (temperature of 20°C and 50% RH) and laboratory (uncontrolled) conditions. Seed samples were taken every three months and submitted to the tests mentioned previously. The harmful effect of fungi on seed quality before storage was observed. Generally, there was a reduction in fungal occurrence with storage, principally for the conditions of the cold chamber and the uncontrolled laboratory environment, resulting in the increased of germination and seedling emergence. 


Author(s):  
Zirvaldo Z. Virgolino ◽  
Osvaldo Resende ◽  
Douglas N. Gonçalves ◽  
Kaique A. F. Marçal ◽  
Juliana de F. Sales

ABSTRACT The aeration of seeds with artificially cooled air and their storage in big-bag packages aim to lengthen the shelf life while maintaining the quality. Thus, the objective was to study the effects of cooling before storage on germination and vigor of soybean seeds stored in trifoliate kraft paper bag and big bag in non-air-conditioned environment. Seeds of soybean cultivar NA 7337 RR were mechanically harvested in March 2013, holding an average moisture content of 18% w.b. In June, 16,000 kg of seeds were processed and cooled to 18 °C. Equal amount of non-cooled seeds was used as a control. Equally divided in kraft paper and in big bags, and combining cooling and packaging, the seeds were stored for three months and analyzed for moisture content, germination and vigor. Cooling to room temperature and different packaging types had similar effects on seed quality. Big bags packing showed better efficiency in retaining the moisture content of cooled seeds. No direct effects of cooling could be identified prior to storage on the germination and vigor of soybean seeds.


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