scholarly journals Mode of analysis of fatty acid molecule binding to the N-terminal 8 kDa domain of DNA polymeraseβ

1999 ◽  
Vol 39 (supplement) ◽  
pp. S176
Author(s):  
Y. Mizushina ◽  
T. Ohkubo ◽  
T. Date ◽  
T. Yamaguchi ◽  
M. Saneyoshi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Molecule ◽  
Fatty Acid Molecule

An optical method of measuring the thickness of adsorbed monolayers

1952 ◽  
Vol 212 (1111) ◽  
pp. 505-508 ◽  
Keyword(s):  
Fatty Acid ◽  
Solid Surface ◽  
Optical Method ◽  
Solid Surfaces ◽  
Acid Molecule ◽  
Fatty Acid Molecule ◽  
Monomolecular Layer ◽  
Independent Evidence ◽  
X Ray

The interferometric techniques developed by Tolansky have been used to study films adsorbed on solid surfaces. A monomolecular layer of fatty acid was spread by the retraction technique over part of a selected facet of a piece of mica. A highly reflecting layer of silver was then deposited on both sides of the mica specimen, and the thickness of the acid layer determined by multiple reflexion interferometry. The values so obtained were in agreement with X-ray data on the length of the fatty-acid molecule. Examination showed that the layers were uniform in thickness; polymolecular layers were absent. The method provides direct and independent evidence that molecules of a fatty acid, spread by the retraction technique, are adsorbed on a solid surface as a uniform monomolecular layer.

scholarly journals Mode Analysis of a Fatty Acid Molecule Binding to the N-terminal 8-kDa Domain of DNA Polymerase β

1999 ◽  
Vol 274 (36) ◽  
pp. 25599-25607 ◽  
Author(s):  
Yoshiyuki Mizushina ◽  
Tadayasu Ohkubo ◽  
Takayasu Date ◽  
Toyofumi Yamaguchi ◽  
Mineo Saneyoshi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Dna Polymerase ◽  
Mode Analysis ◽  
Acid Molecule ◽  
Fatty Acid Molecule ◽  
Dna Polymerase Β

The fatty acid molecule

1924 ◽  
Vol 43 (31) ◽  
pp. 782-784
Author(s):  
T. W. Jones
Keyword(s):  
Fatty Acid ◽  
Acid Molecule ◽  
Fatty Acid Molecule

Inhibition of Phytophthora cactorum by a bacterial antagonist

1983 ◽  
Vol 61 (12) ◽  
pp. 3343-3348 ◽  
Author(s):  
R. S. Utkhede ◽  
A. P. Gaunce
Keyword(s):  
Molecular Weight ◽  
Low Ph ◽  
Low Molecular Weight ◽  
Ion Exchange Resins ◽  
Field Soil ◽  
Acid Molecule ◽  
Phytophthora Cactorum ◽  
Fatty Acid Molecule ◽  
Cornmeal Agar ◽  
Exchange Resins

Bacterial iolate B8 inhibited the growth of six isolates of Phytophthora cactorum on cornmeal agar. Under greenhouse conditions, B8 reduced infections by three isolates of P. cactorum in sterile field soil. Growth of P. cactorum on cornmeal agar was completely inhibited with 40% autoclaved B8 extract. The growth was significantly reduced by low pH alone (pH 4.5 or less) but even when the pH of B8 extract was raised to 6, P. cactorum growth was completely inhibited. Cornmeal agar buffered with 15 mM citrate, pH 5, was suitable for assaying extracts for antagonism to P. cactorum. Acetic acid and other fatty acids (possible metabolites of B8) were also inhibitory, with degree of antibiosis increasing with the size of the fatty acid molecule. The antibiotic produced by B8 was characterized as neutral (not absorbed by ion exchange resins) and of low molecular weight.

scholarly journals Stereochemical requirements for the modulation by retinoic acid of thyroid hormone activation of Ca2+-ATPase and binding at the human erythrocyte membrane

1992 ◽  
Vol 284 (2) ◽  
pp. 583-587 ◽  
Author(s):  
T J Smith ◽  
F B Davis ◽  
P J Davis
Keyword(s):  
Retinoic Acid ◽  
Fatty Acid ◽  
Thyroid Hormone ◽  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Binding Sites ◽  
Erythrocyte Membranes ◽  
Side Chain ◽  
Acid Molecule

Physiological concentrations of retinoic acid can block the activation of human erythrocyte Ca(2+)-ATPase in vitro by thyroid hormone [Smith, Davis & Davis (1989) J. Biol. Chem. 264, 687-689]. The present studies were undertaken to ascertain the nature of this blockade. Two binding sites for L-thyroxine (T4) were demonstrated on washed erythrocyte membranes; the high-affinity site had a Kd value of 2.7 x 10(-10)M and a Bmax. of 76 fmol/mg of protein. The lower-affinity site possessed a Kd of 1 x 10(-8) M. Retinoic acid was as potent a displacer of radiolabelled T4 as was the unlabelled hormone. Certain retinoic acid analogues with either ring or fatty acid side chain modifications retained some ability to displace [125I]T4 binding and to block iodothyronine activation of Ca(2+)-ATPase. The side chain terminal carboxyl group was essential for full activity of the retinoic acid molecule. Its absence or replacement with an ethylsulphone group rendered the molecule considerably less active in the ATPase model. Retinol, 13-cis-retinoic acid, benzene-substituted all-trans-retinoic acid and polyprenoic acid all failed to influence iodothyronine binding or to block activation of Ca(2+)-ATPase by T4. There was good agreement between the ability of an analogue to displace [125I]iodothyronine binding and its ability to inhibit the T4-dependent activation of the Ca(2+)-ATPase. It would appear from these observations that retinoic acid can modulate the activation of erythrocyte membrane Ca(2+)-ATPase by thyroid hormone through a mechanism which involves displacement of iodothyronine from binding sites. These activities apparently derive from both the ring structure and the fatty acid side chain of the retinoic acid molecule.

scholarly journals Uptake of N-(9-Anthroyloxy) Fatty Acid Fluorescent Probes Into Lipid Bilayers

1978 ◽  
Vol 31 (5) ◽  
pp. 447 ◽  
Author(s):  
Elizabeth A Haigh ◽  
Keith R Thulborn ◽  
Lawrence W Nichol ◽  
William H Sawyer
Keyword(s):  
Fatty Acid ◽  
Lipid Bilayers ◽  
Binding Sites ◽  
Electrostatic Interactions ◽  
Fluorescence Enhancement ◽  
Phospholipid Bilayers ◽  
Fatty Acid Molecule ◽  
Membrane Interaction ◽  
Free Energy Of Binding ◽  
Partition Process

A description in thermodynamic terms is given of ligand-membrane interaction which may occur by either or both a binding and a partition process. Results obtained by fluorescence enhancement and polarization techniques on the uptake of n-(9-anthroyloxy) fatty acids by phospholipid bilayers are analysed to show that binding rather than partition effects primarily determine the extent of probe uptake. Liposome concentration-dependence effects are also reported which required that binding results obtained with different probes be compared at fixed lipid concentrations. On this basis it is concluded that as the separation of the anthracene and carboxyl groups within the fatty acid molecule is increased, and hence as the anthracene group moves deeper into the bilayer, the fluorescent probe is bound to the bilayer with greater affinity but is accepted by fewer binding sites within the membrane. Studies on probe uptake at high ionic strength and into negatively charged bilayers indicate that hydrophobic rather than electrostatic interactions make the dominant contribution to the free energy of binding.

Temperature Dependence of the Critical Electron Dose for Fatty Acid Monolayers

1982 ◽  
Vol 40 ◽  
pp. 78-79
Author(s):  
Kenneth H. Downing ◽  
Robert M. Glaeser
Keyword(s):  
Electron Beam ◽  
Fatty Acid ◽  
Inelastic Scattering ◽  
Temperature Dependence ◽  
Structural Damage ◽  
Direct Result ◽  
Second Step ◽  
Strong Temperature Dependence ◽  
Electron Dose ◽  
Covalent Bonds

The structural damage of molecules irradiated by electrons is generally considered to occur in two steps. The direct result of inelastic scattering events is the disruption of covalent bonds. Following changes in bond structure, movement of the constituent atoms produces permanent distortions of the molecules. Since at least the second step should show a strong temperature dependence, it was to be expected that cooling a specimen should extend its lifetime in the electron beam. This result has been found in a large number of experiments, but the degree to which cooling the specimen enhances its resistance to radiation damage has been found to vary widely with specimen types.

Effect of light intensity on the ultrastructure of the filamentous cyanobacterium, Anabaena variabilis

1988 ◽  
Vol 46 ◽  
pp. 300-301
Author(s):  
C. S. Bricker ◽  
S. R. Barnum ◽  
B. Huang ◽  
J. G. Jaworskl
Keyword(s):  
Fatty Acid ◽  
Light Intensity ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Anabaena Variabilis ◽  
Chloroplast Envelope ◽  
Major Constituent ◽  
Filamentous Cyanobacterium ◽  
Photosynthetic Membrane ◽  
Effect Of Light

Cyanobacteria are Gram negative prokaryotes that are capable of oxygenic photosynthesis. Although there are many similarities between eukaryotes and cyanobacteria in electron transfer and phosphorylation during photosynthesis, there are two features of the photosynthetic apparatus in cyanobacteria which distinguishes them from plants. Cyanobacteria contain phycobiliproteins organized in phycobilisomes on the surface of photosynthetic membrane. Another difference is in the organization of the photosynthetic membranes. Instead of stacked thylakolds within a chloroplast envelope membrane, as seen In eukaryotes, IntracytopIasmlc membranes generally are arranged in three to six concentric layers. Environmental factors such as temperature, nutrition and light fluency can significantly affect the physiology and morphology of cells. The effect of light Intensity shifts on the ultrastructure of Internal membrane in Anabaena variabilis grown under controlled environmental conditions was examined. Since a major constituent of cyanobacterial thylakolds are lipids, the fatty acid content also was measured and correlated with uItrastructural changes. The regulation of fatty acid synthesis in cyanobacteria ultimately can be studied if the fatty acid content can be manipulated.

The effects of low-ratio n-6/n-3 PUFA on biomarkers of inflammation: a systematic review and meta-analysis

2021 ◽  
Author(s):  
Yali Wei ◽  
Yan Meng ◽  
Na Li ◽  
Qian Wang ◽  
Liyong Chen
Keyword(s):  
Systematic Review ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Meta Analysis ◽  
Chain Polyunsaturated Fatty Acid ◽  
Inflammation Markers ◽  
Pufa Supplementation ◽  
Long Chain

The purpose of the systematic review and meta-analysis was to determine if low-ratio n-6/n-3 long-chain polyunsaturated fatty acid (PUFA) supplementation affects serum inflammation markers based on current studies.

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