scholarly journals Observation of interaction between alphalactalbumin and calcium ion by mechanochemical method

2003 ◽  
Vol 43 (supplement) ◽  
pp. S93
Author(s):  
Y. Yamagata ◽  
K. Mogami ◽  
H. Aoki ◽  
B.H. Lee ◽  
J. Kim ◽  
...  
Author(s):  
J.R. Walton

In electron microscopy, lead is the metal most widely used for enhancing specimen contrast. Lead citrate requires a pH of 12 to stain thin sections of epoxy-embedded material rapidly and intensively. However, this high alkalinity tends to leach out enzyme reaction products, making lead citrate unsuitable for many cytochemical studies. Substitution of the chelator aspartate for citrate allows staining to be carried out at pH 6 or 7 without apparent effect on cytochemical products. Moreover, due to the low, controlled level of free lead ions, contamination-free staining can be carried out en bloc, prior to dehydration and embedding. En bloc use of lead aspartate permits the grid-staining step to be bypassed, allowing samples to be examined immediately after thin-sectioning.Procedures. To prevent precipitation of lead salts, double- or glass-distilled H20 used in the stain and rinses should be boiled to drive off carbon dioxide and glassware should be carefully rinsed to remove any persisting traces of calcium ion.


2020 ◽  
pp. 59-64
Author(s):  
N. I. Kurbanova ◽  
◽  
T. M. Gulieva ◽  
N. Ya. Ischenko ◽  
◽  
...  

The effect of additives of nanofillers (NF) containing nanoparticles (NP) of copper oxide, stabilized by a polymer matrix of maleized polyethylene (MPE), obtained by the mechanochemical method, on the properties of composites based on isotactic polypropylene (PP) and high-pressure polyethylene (PE) was studied by X-ray phase (XRD) and thermogravimetric (TGA) analyzes. The enhancement of strength, deformation, and rheological parameters, as well as the thermo-oxidative stability of the obtained nanocomposites was revealed, which, apparently, is due to the synergistic effect of the interaction of copper-containing nanoparticles with anhydride groups of MPE. It is shown that nanocomposites based on PP/PE/NF can be processed both by pressing and injection molding and extrusion, which expands the scope of its application.


2020 ◽  
Vol 23 (05) ◽  
pp. 07-10
Author(s):  
Zahraa Ch. Hameed ◽  
Aiyat Alshugary ◽  
Naseer Abdul Ameer
Keyword(s):  

2004 ◽  
Vol 4 (5-6) ◽  
pp. 223-231
Author(s):  
H.-H. Yeh ◽  
W.-H. Wang

The utilization of membrane processes for drinking water treatment has become more popular. However, fouling by source water probably is the major factor prohibits its widespread application. In this research, the fouling phenomena of a microfiltration (MF) membrane were studied. The interactions among colloidal particles, calcium ion, and dissolved organics, such as salicylic acid, humic acid, and alginic acid, on MF fouling were focused. A lab-scale single hollow fiber MF membrane, made of polyvinylidenefluoride (PVDF), module was used. The results show that, for single organic compound, the extent of fouling caused by humic acid was higher that of alginic acid. For the latter, the permeate flux decrease at lower pH was more significant than those at higher pH. For low MW salicylic acid, both rejection and flux decrease were minor. It seems that solubility have strong correlation with fouling rate. The higher the solubility is, the lower the fouling rate. For sole colloidal particle system, latex beads with diameter close to the pore size of MF membrane showed severe fouling. Adding Ca can aggregate the latex beads, and alleviate fouling. However, calcium ion also found to increase fouling of alginic acid on membrane under neutral or alkali pH condition, probably via charge neutralization and/or bridging. In conclusion, MF fouling seems to be strongly related to the type of organics, size of colloidal particles, and the existence of divalent ions, in the feed water.


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