PERCENTAGE VALUE OF MOTILITY AND VIABILITY SPERMATOZOA IN BOER GOAT FROZEN SEMEN WERE ADDED STREPTOMICIN AND SWEET ORANGE ESSENTIAL OIL

One of factors that cause a bad quality of Boer Goat frozen semen is the growth of bacterial. This can be overcome by adding antibiotics such as streptomycin. To further suppress the growth of bacteria can be added other ingredients that contain antibacterials such as sweet orange essential oil. The purpose of this research is to know the percentage value of Membrane Integrity and Acrosome Integrity on Boer Goat frozen semen with addition sweet orange essential oil and streptomycin. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment in this research is addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and streptomycin extender. The results showed the best treatment addition combination streptomycin and sweet orange essential oil to percentage Membrane Integrity and Acrosome Integrity is increase 1% sweet orange essential oil.Keywords: Boer Goat, essential oil, frozen semen, streptomycin, sweet orange.

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Spermatozoa Abnormality Test of Boer Goats Frozen Semen with the Addition of Sweet Orange Essential Oil and Streptomycin in Tris Yolk Extender

Asian Journal of Advances in Agricultural Research ◽

10.9734/ajaar/2020/v12i230076 ◽

2020 ◽

pp. 20-23

Author(s):

Sukma Aditya Sitepu

Keyword(s):

Essential Oil ◽

Sweet Orange ◽

Egg Yolk ◽

Boer Goat ◽

Average Value ◽

Frozen Semen ◽

Before And After ◽

Boer Goats ◽

Fresh Semen ◽

Orange Essential Oil

This research aims to to determine the percentage value of spermatozoa abnormalities in Boer Goat frozen semen by adding a combination of streptomycin with sweet orange essential oil to tris yolk extender. The ingredients used in this study were fresh semen from Boer Goat, tris yolk extender, streptomycin, and sweet orange essential oil. Tris yolk extender is made using Tris (hydroxymethylaminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquabides (100 ml). The study design was a non factorial completely treatments given were the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1%. The results showed that the addition of a combination of streptomycin and sweet orange essential oil into the tris yolk extender had a highly significant effect (P <0.01) on the percentage value of spermatozoa abnormalities of Boer Goats before and after freezing. The best average value of spermatozoa abnormalities is the addition of sweet orange essential oil at 1% (P4) with the percentage of spermatozoa abnormalities before freezing at 3% and after freezing at 6%.

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Improved quality of frozen boer goat semen with the addition of sweet orange essential oil on tris yolk and gentamicin extender

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/122/1/012125 ◽

2018 ◽

Vol 122 ◽

pp. 012125 ◽

Cited By ~ 1

Author(s):

S A Sitepu ◽

U Zaituni ◽

Jaswandi ◽

Hendri

Keyword(s):

Essential Oil ◽

Sweet Orange ◽

Boer Goat ◽

Orange Essential Oil

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Utilization Of Frozen Goat Semen With Addition Sweet Orange Essential Oil To Improve Genetic Quality in Ujung Teran Village

Journal of Saintech Transfer ◽

10.32734/jst.v1i2.839 ◽

2019 ◽

Vol 1 (2) ◽

pp. 170-174

Author(s):

Sukma Aditya Sitepu ◽

Julia Marisa

Keyword(s):

Essential Oil ◽

Community Service ◽

Artificial Insemination ◽

Sweet Orange ◽

Genetic Quality ◽

Know How ◽

Frozen Semen ◽

And Training ◽

Orange Essential Oil

One of the causes of underdeveloped goats is low genetic quality. The way that can be done to improve the genetic quality of goats is to do cross-breeding with superior goats through Artificial Insemination. The community service aims to increase the knowledge of goat farmers in Ujung Teran Village, Langkat Regency on how to make goat frozen semen using tris yolk extender plus sweet orange essential oil for Artificial Insemination program along with the benefits that can be obtained. The method of service is by giving lectures and training to goat farmers. The results obtained from the implementation of this program are that farmers know how to make frozen semen of goat using sweet orange essential oil on the tris yolk extender and are willing to make Artificial Insemination in their goat cattle.

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Total Bacteria of Post-Thawing Boer Buck Semen with Addition of Sweet Orange Essential Oil to Tris Yolk Extender

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2020/v5i330129 ◽

2020 ◽

pp. 1-5

Author(s):

Sukma Aditya Sitepu ◽

Zaituni Udin ◽

. Jaswandi ◽

. Hendri

Keyword(s):

Essential Oil ◽

Sweet Orange ◽

Block Design ◽

Randomized Block Design ◽

Frozen Semen ◽

Semen Storage ◽

Research Procedure ◽

Semen Extender ◽

Total Bacteria ◽

Orange Essential Oil

Aims: To determine the effect of the addition of sweet orange essential oil to the extender (tris yolk extender without the addition of antibiotics/antibacterial) to total bacteria Boer Buck frozen semen. Study Design: Randomized Block Design. Place and Duration of Study: Sample: Laboratory Reproduction of Loka Penelitian Kambing Potong Sei Putih Indonesia, between January and March 2019. Methodology: The research procedure starts with the preparation of semen extender, collection of fresh semen, dilution of semen, equilibration, freezing of semen, and thawing. This research obtained conducted using a Randomized Block Design consisting of 5 treatment levels and five replications. Semen storage using 3 Boer buck, which done for three days. As a treatment is the addition of sweet orange essential oil as much as (P0) 0%, (P1) 0,25%, (P2) 0,5%, (P3) 0,75% and (P4) 1% on the tris yolk extender. The observed variables was total bacteria evaluated before freezing and after freezing (Post-Thawing). Results: The results showed that the addition of sweet orange essential oil had a very significant effect (P <0.01). The results of adding sweet orange essential oil to the extender (Post-Thawing) were 78.6 x 102 CFU (P0), 76 x 102 CFU (P1), 73,2 x 102 CFU (P2), 71 x 102 CFU (P3) and 68.6 x 102 CFU (P4). Conclusion: The best values the total bacteria 68.6 x 102 CFU (P4). It can conclude that the best result of adding sweet orange essential oil to tris yolk extender is (P4) 1%.

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QUALITY DIFFERENCES OF BOER LIQUID SEMEN DURING STORAGE WITH ADDITION SWEETORANGEESSENTIAL OIL IN TRIS YOLK AND GENTAMICIN EXTENDER

Journal of Community Research and Service ◽

10.24114/jcrs.v1i2.9341 ◽

2018 ◽

Vol 1 (2) ◽

pp. 78 ◽

Cited By ~ 2

Author(s):

Sukma Aditya Sitepu ◽

Zaituni Udin ◽

Jaswandi Jaswandi ◽

Hendri Hendri

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Room Temperature ◽

Semen Quality ◽

Sweet Orange ◽

Orange Peel ◽

Boer Goat ◽

Closed Tube ◽

Growth Of Bacteria

AbstractThe purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange

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