scholarly journals STUDIES ON VARIABILITY OF MORPHOLOGICAL CHARACTERS ON JAMUN (SYZYGIUM CUMINII SKEELS) GENOTYPES GROWING IN NORTHERN BASTAR PLATEAU REGION OF CHHATTISGARH

2020 ◽  
Vol 8 (10) ◽  
pp. 45-49
Author(s):  
Chongtham Allaylay Devi ◽  
◽  
G.L. Sharma ◽  
Prabhakar Singh ◽  
H.K. Panigrahi ◽  
...  

Surveys were undertaken for characterization and evaluation of genetic diversity of Jamun (SyzygiumcuminiiSkeels) of seedling origin for different morphological characters of tree characters and leaf characters during 2016-17 and 2017-18 in the Jamun growing in Northern Bastar Plateau region of Chhattisgarh areas. Investigations were undertaken during the entire vegetative phase to assess the distribution, range and to record the range of genetic variability of different morphological traits on the selected sixty trees. Mature leaves were plucked and analyzed for different parameters such as leaf surface and leaf glands. Results of evaluated Jamun genotypes showed wide variability for studied characteristic.

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
R.A. PATIL ◽  
S.G. BHARAD ◽  
S.N. SAWANT

Assessment of genetic diversity in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In the view of this Forty-eight genotypes of seedling origin guava along with 1 check (L-49/Sardar) collected and conserved at germplasm block, Main Garden, Department of Horticulture, Dr. P. D. A. University, Akola were evaluated for genetic variability and diversity based on the qualitative characteristics. The genotypes were evaluated for sixteen morphological traitsviz. tree, leaf, floral and fruit traits. Results Show considerable extent of variability amongst the 49 genotypes in each traits. A sizeable amount of intrapopulation diversity recorded can be used to identify diverse parents which can be utilized in hybridization programmes.


2017 ◽  
pp. 45-58
Author(s):  
ZAKIR ULLAH

A total of 60 genotypes of Brassica campestris (L) germplasm were acquired from National Research Centre (NARC) Islamabad, Pakistan for the evaluation of genetic diversity using the morphological characters and SDS-PAGE analysis. These genotypes were grown-up in Plant Garden Department of plant sciences, University of Malakand, Khyber Pukhtunkhwa, Pakistan. For the assessment of genetic diversity total of 18 morphological traits were scored and their phylogenetic relationship were plot through cluster diagram. For the estimation of genetic diversity, we used the most influential technique SDS PAGE. We observed six loci (bands) from the collected genotypes; locus-1 (band-1) contained 100% bands of protein and obvious as monomorphic locus while loci i.e. 2,3,4,5 and 6 showed 28%, 42%, 36%, 56% and 25%, respectively variation and were polymorphic. The inter species locus influence toward genetic assortment (LCTGA) was 83.33% in the attained genotypes. Profiling through SDS PAGE based on two-way cluster plottingresolved effectively the collected genotype into 4 cluster. We consider that this work provides a baseline for the understanding genetic diversity in the common crops used in Pakistan and warrants further investigation in this newly evolving field of study.


Author(s):  
Narendra Singh Rajpoot ◽  
M. K. Tripathi ◽  
Sushma Tiwari ◽  
R. S. Tomar ◽  
V. S. Kandalkar

The genus Brassica is one of the most important oil seed crops in India with high degree of genetic diversity. In present study, genetic diversity was studied in forty germplasm lines and eight cultivars of Indian mustard using morphological traits and SSR markers. Morphological characters were taken for days to 50% flowering, days to maturity, plant height (cm), length of main raceme (cm), number of primary branches/plant, number of secondary branches/plant, number of silique per plant, number of seeds per silique, 1000 seed weight (g) and seed yield per plant (g). Total 50 SSR markers were used for characterization of these lines, out of which 7 SSR markers were highly polymorphic between all the germplasms of mustard. An UPGMA phonogram was constructed for all 48 Germplasms and the similarity coefficient ranged from 0.00 to 0.91. Number of alleles ranged from 3 to 4, genetic diversity ranged from 71% to 65% with average value of 67%, heterozygosity raged from 20 to 10% with average of 12% and PIC value for markers ranged from 0.65 to 0.59 with mean PIC value 0.61. All seven SSR primers showed PIC value above 0.5 (50%) indicating high genetic diversity in the studied plant material.


2021 ◽  
Vol 948 (1) ◽  
pp. 012004
Author(s):  
I W Mulsanti ◽  
A Risliawati ◽  
N Yunani

Abstract The present study was carried out to characterize 103 Indonesian local rice germplasm on the basis of 20 agro-morphological traits. The local rice germplasm originated from Riau and Jambi province in Sumatera island. Principal Component Analysis (PCA) was used to analyzed 11 quantitative data. PCA explained the genetic diversity of the rice germplasm accession. Most of the morphological characters showed variation in different accession. PC1 and PC2 explained about 32.5% and 22.1% of the variability, respectively. PCI and PC2 mostly related with traits such as productive tiller number (PTN), vegetative tiller number (VTN), plant height (PH), and culm length (CL). PCA-Biplot showed accession from Jambi and Riau manage to be separated, even though the PCA percent is only 54.6%. Accession originated from Riau marked by number of tillers (PTN, VTN), and grain weight (GW) traits. Based on cluster analysis, rice germplasm grouped in to 4 main clusters. Most of the accession from Jambi and Riau grouped in the same cluster, which is cluster I. Whereas eight local accessions from Riau grouped in the same cluster separately (cluster II). Two accession which are Padi Jarum (Acc 2711) and Ketan Hitam (Acc 9300) are individually, separated from others.


2014 ◽  
Vol 50 (No. 2) ◽  
pp. 177-184 ◽  
Author(s):  
B. Gixhari ◽  
M. Pavelková ◽  
H. Ismaili ◽  
H. Vrapi ◽  
A. Jaupi ◽  
...  

In order to investigate the genetic diversity present in the pea germplasm stored in the Albanian genebank, we analyzed 28 local pea genotypes of Albanian origins for 23 quantitative morphological traits, as well as 14 retrotransposon-based insertion polymorphism (RBIP) molecular markers. The study of morphological characters carried out during three growing seasons (2010, 2011 and 2012) had the objective of characterization of traits useful in breeding programs. RBIP marker analysis revealed the genetic similarity in range from 0.06 to 0.45. ANOVA, principal component analysis (PCA) and cluster analysis was used to visualize the association among different traits. Most of the quantitative morphological traits showed significant differences. PCA and cluster analysis (Ward’s method) carried out for morphological traits divided the local pea genotypes into three clusters. Finally, the study identified the agronomicaly important traits which will facilitate the maintenance and agronomic evaluation of the collections.


Genetika ◽  
2017 ◽  
Vol 49 (2) ◽  
pp. 543-557 ◽  
Author(s):  
Somayeh Bozchaloyi ◽  
Masoud Sheidai ◽  
Maryam Keshavarzi ◽  
Zahra Noormohammadi

Genetic variability and populations, structure were studied in 15 geographical populations of Geranium purpureum Vill. (Geraniaceae). Genetic diversity parameters were determined in these populations. AMOVA and Gst analyses revealed the presence of genetic variability within populations and significant molecular difference among the studied populations. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. STRUCTURE and K-Means clustering revealed populations, genetic stratification. UPGMA dendrogram of populations based on morphological characters was in agreement with NJ tree of molecular data. These results indicated that geographical populations of G. purpureum are well differentiated both in genetic content as well as morphological characteristics. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of ecotypes within this species.


2021 ◽  
Author(s):  
Luis Augusto Becerra Lopez-Lavalle ◽  
Adriana Bohorquez-Chaux ◽  
Xiaofei Zhang

The identification of cassava cultivars is important for understanding the crop’s production system, enabling crop improvement practitioners to design and deliver tailored solutions with which farmers can secure high yields and sustainable production. Across the lowland tropics today, a large number improved varieties and landraces of cassava are under cultivation, making it inefficient for breeders and geneticists to set improvement goals for the crop. The identification and characterization of cassava genotypes is currently based on either morphological characters or molecular features. The major aim of cultivar identification is to catalog the crop’s genetic diversity, but a consensus approach has still not been established. Of the two approaches to the identification of variety, morphological characters seem to account for most of the genetic variability reported in cassava. However, these characters must be treated with caution, as phenotypic changes can be due to environmental and climatic conditions as well as to the segregation of new highly heterozygous populations, thus, making the accurate identification of varieties difficult. The use of molecular markers has allowed researchers to establish accurate relationships between genotypes, and to measure and track their heterozygous status. Since the early 1990’s, molecular geneticists working with cassava have been developing and deploying DNA-based tools for the identification and characterization of landraces or improved varieties. Hence, in the last five years, economists and social scientists have adopted DNA-based variety identification to measure the adoption rates of varieties, and to support the legal protection of breeder’s rights. Despite the advances made in the deployment of molecular markers for cassava, multiple platform adoption, as well as their costs and variable throughput, has limited their use by practitioners of crop improvement of cassava. The post-genomic era has produced a large number of genome and transcriptome sequencing tools, and has increased our capacity to develop and deploy genome-based tools to account for the crop’s genetic variability by accurately measuring and tracking allele diversity. These technologies allow the creation of haplotype catalogs that can be widely shared across the cassava crop improvement community. Low-density genome-wide SNP markers might be the solution for the wide adoption of molecular tools for the identification of cultivars or varieties of cassava. In this review we survey the efforts made in the past 30 years to establish the tools for cultivar identification of cassava in farmer’s fields and gene banks. We also emphasize the need for a global picture of the genetic diversity of this crop, at its center of origin in South America.


2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Md. Mia Mukul ◽  
Nargis Akter ◽  
Md. Golam Mostofa ◽  
Md. Sohanur Rahman ◽  
Md. Al-Emran Hossain ◽  
...  

An investigation was done to assess the genetic variability, character associations, and genetic diversity of eight jute genotypes for seven morphological traits in a randomised complete block design at Bangladesh Jute Research Institute during 15 March, 2018 to 31 December, 2019. Analyses results revealed significant differences (P<0.01) among all genotypes for studied traits indicating the presence of variability. All the lines performed better than one control (JRO-524), and the line (O-0412-9-4) provided good results for desired traits than all controls. Jute fibre yield showed the highest broad sense heritability (98.54%). The studied jute morphological traits i.e. Plant population, the plant height, green weight, dry fibre yield and dry stick yield gave high heritability along with high genotypic and phenotypic variances, genetic advances in percent of the mean, highly significant and positive correlations. It indicates the possibility of crop improvement through phenotypic selection and maximum genetic gain, simultaneously at the genotypic-phenotypic level. Clustering analysis grouped all genotypes into three distinct clusters. The cluster II showed the highest mean values for all traits followed by cluster I and III. The first two principal components with higher Eigen values (>1.0) accounted for 90.88% of the total variation in the principal component analysis. PCA and cluster analyses indicated that the advanced breeding line O-0412-9-4 made its individual cluster II with higher inter-cluster distance and higher fibre yield (3.12 t ha-1). The investigation was done to select the genotype(s) with good fibre yield and distinct features in respect of developing high yielding Tossa jute variety for cultivation in the farmers’ field. This genotype O-0412-9-4 was selected based on higher plant height, base diameter, fibre yield content. It will be developed as a high yielding variety considering its’ higher genetic variability, heritability, genetic advance, significant associations for desirable characters.


2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Kurnia Makmur ◽  
TATIK CHIKMAWATI ◽  
SOBIR SOBIR

Abstract. Makmur K, Chikmawati T, Sobir. 2020. Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesia based on morphological characters and ISSR markers. Biodiversitas 21: 5948-5953. Lemon basil (Ocimum × africanum Lour.) or kemangi is a highly beneficial plant, yet still underutilized. Therefore, providing necessary information on genetic diversity of this species is essential for further utilization. The aim of this study was to elucidate genetic diversity of 33 accessions of O. × africanum collected from four Islands of Indonesia along with three accessions of Ocimum basilicum L. Morphological observation was conducted on 37 morphological characters following the International Union for the Protection of New Varieties of Plants descriptor guidelines; subsequently, 13 ISSR primers were employed in molecular analysis. Both morphological and molecular data were analyzed based on simple matching similarity index using UPGMA method. Morphologically, O. × africanum and O. basilicum were clearly separated at the similarity index of 0.52, and among two species were divided into two groups according to two either character. ISSR analysis using 13 ISSR primers produced 111 DNA bands, and 108 of them (97.29%) were polymorphic. Cluster analysis based on ISSR data could not explicitly separate O. × africanum and O. basilicum accessions. Besides, Ocimum accessions collected from the same area did not always cluster into one group.


2019 ◽  
Vol 94 (3) ◽  
pp. 166
Author(s):  
Pablo Roberto da Silva Ozorio ◽  
André Luiz Atroch ◽  
Firmino José do Nascimento Filho

Based on agro-morphological traits, this study aimed to describe and quantify the genetic diversity between eighteen guarana cultivars from the guarana breeding program, to identify cultivars for use in multiple-purpose in breeding programs to obtain segregating populations with great variability and diversity. Dis-similarity between cultivars was calculated using Gower’s algorithm and a dendrogram was obtained using the UPGMA algorithm. High genetic variability and diversity was identified between guarana cultivars via 20 morpho-agronomic traits. Two groups and six subgroups of genetic diversity were identified. Crosses between clones of different subgroups may produce high variability and diversity in segregating populations, increasing the capacity to select for superior genotypes in these populations.


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