Estimation of genetic diversity in the germplasm of Brassica Campestris (L.) Using morphological and biochemical markers

JOURNAL OF BOTANY ◽

10.33195/uochjb-v1i1332017 ◽

2017 ◽

pp. 45-58

Author(s):

ZAKIR ULLAH

Keyword(s):

Genetic Diversity ◽

Phylogenetic Relationship ◽

Biochemical Markers ◽

Morphological Traits ◽

Brassica Campestris ◽

Morphological Characters ◽

Research Centre ◽

Sds Page ◽

Plant Sciences ◽

The Common

A total of 60 genotypes of Brassica campestris (L) germplasm were acquired from National Research Centre (NARC) Islamabad, Pakistan for the evaluation of genetic diversity using the morphological characters and SDS-PAGE analysis. These genotypes were grown-up in Plant Garden Department of plant sciences, University of Malakand, Khyber Pukhtunkhwa, Pakistan. For the assessment of genetic diversity total of 18 morphological traits were scored and their phylogenetic relationship were plot through cluster diagram. For the estimation of genetic diversity, we used the most influential technique SDS PAGE. We observed six loci (bands) from the collected genotypes; locus-1 (band-1) contained 100% bands of protein and obvious as monomorphic locus while loci i.e. 2,3,4,5 and 6 showed 28%, 42%, 36%, 56% and 25%, respectively variation and were polymorphic. The inter species locus influence toward genetic assortment (LCTGA) was 83.33% in the attained genotypes. Profiling through SDS PAGE based on two-way cluster plottingresolved effectively the collected genotype into 4 cluster. We consider that this work provides a baseline for the understanding genetic diversity in the common crops used in Pakistan and warrants further investigation in this newly evolving field of study.

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Characterization of Indian Mustard Germplasm on the Basis of Morphological Traits and SSR Markers

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2020/v39i4831234 ◽

2020 ◽

pp. 300-311

Author(s):

Narendra Singh Rajpoot ◽

M. K. Tripathi ◽

Sushma Tiwari ◽

R. S. Tomar ◽

V. S. Kandalkar

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Morphological Traits ◽

Indian Mustard ◽

Morphological Characters ◽

High Genetic Diversity ◽

Average Value ◽

Seed Crops ◽

High Degree

The genus Brassica is one of the most important oil seed crops in India with high degree of genetic diversity. In present study, genetic diversity was studied in forty germplasm lines and eight cultivars of Indian mustard using morphological traits and SSR markers. Morphological characters were taken for days to 50% flowering, days to maturity, plant height (cm), length of main raceme (cm), number of primary branches/plant, number of secondary branches/plant, number of silique per plant, number of seeds per silique, 1000 seed weight (g) and seed yield per plant (g). Total 50 SSR markers were used for characterization of these lines, out of which 7 SSR markers were highly polymorphic between all the germplasms of mustard. An UPGMA phonogram was constructed for all 48 Germplasms and the similarity coefficient ranged from 0.00 to 0.91. Number of alleles ranged from 3 to 4, genetic diversity ranged from 71% to 65% with average value of 67%, heterozygosity raged from 20 to 10% with average of 12% and PIC value for markers ranged from 0.65 to 0.59 with mean PIC value 0.61. All seven SSR primers showed PIC value above 0.5 (50%) indicating high genetic diversity in the studied plant material.

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Agro-morphological characterization based genetic diversity of Indonesian local rice germplasm

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/948/1/012004 ◽

2021 ◽

Vol 948 (1) ◽

pp. 012004

Author(s):

I W Mulsanti ◽

A Risliawati ◽

N Yunani

Keyword(s):

Genetic Diversity ◽

Principal Component Analysis ◽

Quantitative Data ◽

Morphological Traits ◽

Principal Component ◽

Morphological Characters ◽

Morphological Characterization ◽

Tiller Number ◽

Rice Germplasm ◽

Productive Tiller

Abstract The present study was carried out to characterize 103 Indonesian local rice germplasm on the basis of 20 agro-morphological traits. The local rice germplasm originated from Riau and Jambi province in Sumatera island. Principal Component Analysis (PCA) was used to analyzed 11 quantitative data. PCA explained the genetic diversity of the rice germplasm accession. Most of the morphological characters showed variation in different accession. PC1 and PC2 explained about 32.5% and 22.1% of the variability, respectively. PCI and PC2 mostly related with traits such as productive tiller number (PTN), vegetative tiller number (VTN), plant height (PH), and culm length (CL). PCA-Biplot showed accession from Jambi and Riau manage to be separated, even though the PCA percent is only 54.6%. Accession originated from Riau marked by number of tillers (PTN, VTN), and grain weight (GW) traits. Based on cluster analysis, rice germplasm grouped in to 4 main clusters. Most of the accession from Jambi and Riau grouped in the same cluster, which is cluster I. Whereas eight local accessions from Riau grouped in the same cluster separately (cluster II). Two accession which are Padi Jarum (Acc 2711) and Ketan Hitam (Acc 9300) are individually, separated from others.

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Genetic diversity of Albanian pea (Pisum sativum L.) landraces assessed by morphological traits and molecular markers

Czech Journal of Genetics and Plant Breeding ◽

10.17221/227/2013-cjgpb ◽

2014 ◽

Vol 50 (No. 2) ◽

pp. 177-184 ◽

Cited By ~ 7

Author(s):

B. Gixhari ◽

M. Pavelková ◽

H. Ismaili ◽

H. Vrapi ◽

A. Jaupi ◽

...

Keyword(s):

Genetic Diversity ◽

Cluster Analysis ◽

Molecular Markers ◽

Morphological Traits ◽

Principal Component ◽

Morphological Characters ◽

Insertion Polymorphism ◽

Growing Seasons ◽

And Cluster Analysis

In order to investigate the genetic diversity present in the pea germplasm stored in the Albanian genebank, we analyzed 28 local pea genotypes of Albanian origins for 23 quantitative morphological traits, as well as 14 retrotransposon-based insertion polymorphism (RBIP) molecular markers. The study of morphological characters carried out during three growing seasons (2010, 2011 and 2012) had the objective of characterization of traits useful in breeding programs. RBIP marker analysis revealed the genetic similarity in range from 0.06 to 0.45. ANOVA, principal component analysis (PCA) and cluster analysis was used to visualize the association among different traits. Most of the quantitative morphological traits showed significant differences. PCA and cluster analysis (Ward’s method) carried out for morphological traits divided the local pea genotypes into three clusters. Finally, the study identified the agronomicaly important traits which will facilitate the maintenance and agronomic evaluation of the collections.

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Genetic diversity and phylogenetic relationship among Anabantoidei fish (Anabantiformes) in South Kalimantan, Indonesia based on SDS-PAGE analysis

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d200913 ◽

2019 ◽

Vol 20 (9) ◽

Author(s):

Rani Sasmita ◽

Mabrur Mabrur ◽

UMMY SHALIHA AULIA RAHMY ◽

Badruzsaufari Badruzsaufari

Keyword(s):

Genetic Diversity ◽

Ammonium Sulfate ◽

Phylogenetic Relationship ◽

Soluble Protein ◽

Phylogenetic Trees ◽

Protein Profile ◽

Phylogenetic Reconstruction ◽

Fish Muscle ◽

Freshwater Ecosystem ◽

Sds Page

Abstract. Sasmita R, Mabrur, Rahmy USA, Badruzsaufari. 2019. Genetic diversity and phylogenetic relationship among Anabantoidei fish (Anabantiformes) in South Kalimantan, Indonesia based on SDS-PAGE analysis. Biodiversitas 20: 2519-2527. Protein profile is a molecular marker for diversity and phylogenetic analysis of germplasm, including Anabantoidei fish which is abundant in freshwater ecosystem of South Kalimantan. The aim of this research was to analyze genetic diversity and construct phylogenetic relationship among Anabantoidei fish based on SDS PAGE analysis. Protein was extracted from muscle tissue of six species of Anabantoidei fish and precipitated using Ammonium sulfate salt. Soluble protein content was determined using Bradford assay and then separated based on SDS-PAGE method. Genetic diversity and phylogenetic relationship were constructed using PAST software based on UPGMA method. The results showed that the soluble protein of fish muscle can be precipitated optimally in different ammonium sulfate concentration. Based on SDS-PAGE analysis, 59 different protein bands have been separated from gels with molecular weight ranging from 28.15 to 181.61 kDa. On the protein level, the Anabantoidei fish showed high genetic polymorphism (greater than 90%) with 3, 2 and 5 monomorphic bands on non-precipitated, AS-precipitated and combination between non- and AS-precipitated proteins, respectively. The phylogenetic reconstruction also exhibited that the Anabantoidei fish has the unique phylogenetic trees, especially for the combined protein datasets. This information would be useful for freshwater fish conservation and breeding programs.

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STUDIES ON VARIABILITY OF MORPHOLOGICAL CHARACTERS ON JAMUN (SYZYGIUM CUMINII SKEELS) GENOTYPES GROWING IN NORTHERN BASTAR PLATEAU REGION OF CHHATTISGARH

International Journal of Advanced Research ◽

10.21474/ijar01/11821 ◽

2020 ◽

Vol 8 (10) ◽

pp. 45-49

Author(s):

Chongtham Allaylay Devi ◽

◽

G.L. Sharma ◽

Prabhakar Singh ◽

H.K. Panigrahi ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Morphological Traits ◽

Leaf Surface ◽

Morphological Characters ◽

Plateau Region ◽

Vegetative Phase ◽

Mature Leaves ◽

Wide Variability ◽

Syzygium Cuminii

Surveys were undertaken for characterization and evaluation of genetic diversity of Jamun (SyzygiumcuminiiSkeels) of seedling origin for different morphological characters of tree characters and leaf characters during 2016-17 and 2017-18 in the Jamun growing in Northern Bastar Plateau region of Chhattisgarh areas. Investigations were undertaken during the entire vegetative phase to assess the distribution, range and to record the range of genetic variability of different morphological traits on the selected sixty trees. Mature leaves were plucked and analyzed for different parameters such as leaf surface and leaf glands. Results of evaluated Jamun genotypes showed wide variability for studied characteristic.

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Evaluation of Genetic Diversity in Ocimum spp. using Agro-morphological Traits and RAPD Marker

Bhartiya Krishi Anusandhan Patrika ◽

10.18805/bkap348 ◽

2021 ◽

Author(s):

Sachin Kumar ◽

Ajay Kumar ◽

Manoj Kumar Sharma ◽

Manoj Kumar Sharma

Keyword(s):

Genetic Diversity ◽

Leaf Area ◽

Mitotic Index ◽

Morphological Traits ◽

Rapd Markers ◽

Rapd Marker ◽

Morphological Characters ◽

Leaf Length ◽

Number Of Leaves ◽

High Degree

In the present study, morphological and genetic diversity is revealed among the four species of Ocimum. Among the morphological characters plant height, the number of leaves, leaf area, leaf width, leaf length, stem circumference and mitotic index studied and contributed a greater proportion of variations. RAPD markers revealed a high degree of polymorphism (88.89%) among the species of Ocimum. Dendrogram study revealed that the species O. gratissimum and O. basilicum are very closely related whereas O. kilimandscharicum and O. sanctum are distantly related in the phylogenetic history.

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Genetic Diversity and Breeding Potential from Disruptive Selection in Brassica campestris CV Brown Sarson

Experimental Agriculture ◽

10.1017/s001447970000836x ◽

1978 ◽

Vol 14 (1) ◽

pp. 73-79 ◽

Cited By ~ 2

Author(s):

V. Arunachalam ◽

R. K. Katiyar

Keyword(s):

Genetic Diversity ◽

Large Scale ◽

Brassica Campestris ◽

Morphological Characters ◽

Directional Selection ◽

Yield Potential ◽

Disruptive Selection ◽

Breeding Potential ◽

Brown Sarson

SUMMARYNew productive variants (PV) with physiological and morphological characters distinctly different from their original counterparts (OR) were isolated under disruptive selection in Brassica campestris cv Brown Sarson. Two new breeding approaches were tested—mating (PV × OR) F1 lines with selected cultivars, and mating disruptive with two-directional selection lines. The resulting superior three- and two-way hybrid populations, when advanced through large-scale inter-mating, produced double the yield potential of the existing controls. The genetic principles behind these breeding approaches are discussed.

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PROFILING OF GUAVA GENOTYPES GROWN IN VIDARBHA CONDITIONS ON THE BASIS OF MORPHOLOGICAL TRAITS

FLORA AND FAUNA ◽

10.33451/florafauna.v23i1pp37-44 ◽

2017 ◽

Vol 23 (1) ◽

Author(s):

R.A. PATIL ◽

S.G. BHARAD ◽

S.N. SAWANT

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Morphological Traits ◽

Considerable Extent ◽

Fruit Traits ◽

Breeding Programmes ◽

Tree Leaf ◽

Qualitative Characteristics ◽

Intrapopulation Diversity

Assessment of genetic diversity in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In the view of this Forty-eight genotypes of seedling origin guava along with 1 check (L-49/Sardar) collected and conserved at germplasm block, Main Garden, Department of Horticulture, Dr. P. D. A. University, Akola were evaluated for genetic variability and diversity based on the qualitative characteristics. The genotypes were evaluated for sixteen morphological traitsviz. tree, leaf, floral and fruit traits. Results Show considerable extent of variability amongst the 49 genotypes in each traits. A sizeable amount of intrapopulation diversity recorded can be used to identify diverse parents which can be utilized in hybridization programmes.

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