scholarly journals Biodegradation potential of bacterial isolates from dye wastewater at Marina, Sokoto metropolis

2020 ◽  
Vol 36 (2) ◽  
pp. 87-94
Author(s):  
M.A. Adegbite ◽  
K. Ibrahim ◽  
S.A. Yusif
Keyword(s):  
Waste Water ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacterial Species ◽  
Waste Water Treatment ◽  
Dye Wastewater ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Promising Tool ◽  
Degradation Ability

This study was carried out to determine the biodegradation potential of bacterial isolates from dye wastewater at Marina Area, Sokoto. Three (3) of the bacteria were identified; Bacillus subtilis, Luteimonasaestuarrii and Bacillus cereus and were used for further studies based on their degradation ability of green, red, yellow and blue dyes. Bacillus subtilis, Luteimonas aestuarii and Bacillus cereus have the potential to degrade all the four dyes used during the present investigation. However, efficacy of the various bacterial strains was found to vary at different concentration levels in degradation of a particular dye. Thus, by this study it can be concluded that Bacillus cereus, Bacillus subtilis and Luteimonas aestuarii can be used as a good microbial source for dye waste water treatment. The selected bacterial species represent a promising tool for application in biodegradation of dye waste water and the potential observed would allow for the application of the bacterial isolates for treatment of dye effluents before disposal. Keywords: effluents, isolates, biodegradation, dye and bacterial.

scholarly journals Biodegradation of used Engine Oil Contaminated Site using Individual and Mixed bacterial isolates

2020 ◽  
pp. 38-45
Author(s):  
Muh'd A. J. ◽  
Sa'adatu A.Y. ◽  
Surayya M. M. ◽  
Sa'adatu M. I. ◽  
Nafisa B. ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Contaminated Soil ◽  
Bacterial Species ◽  
Soil Samples ◽  
Engine Oil ◽  
Contaminated Site ◽  
Bacterial Isolates ◽  
The World ◽  
Degradation Ability

Oil is most widely distributed source of energy in the world and large-scaled environmental pollutant. Oil, oil products, and oil containing industrial waste pollution is ranked second place after radioactive pollution on account of their harmful action to ecosystems. Contamination of soil by organic chemicals (mostly hydrocarbons) is prevalent in oil producing and industrialized countries of the world. Biodegradation, a strategy that uses biological means (i.e microbes) to degrade, stabilize and remove soil contaminants is an alternative green technology remediation of hydrocarbon contaminated soil. This study was carried out to isolate and screen Bacteria capable of degrading used engine oil from oil contaminated site. Soil samples were collected from oil contaminated site. Bacterial species were isolated from the collected soil samples by serial dilution and agar methods. Different bacterial species were isolated but only four were oil degrading isolates. The identity of the various genera of bacterial contaminants were determined by a combination of cultural, morphological as well as preliminary biochemical characteristics of the isolates. The four oil degrading bacteria (AJ1, AJ2, AJ3 and AJ4) were preliminarily identified as Bacillus cereus, Pseudomonas aeruginosa, Bacillus subtillis and Micrococcus spp respectively. The degradation ability of the bacterial isolates was screened and maximum degradation was recorded by AJ 5 (Mixed culture) with 66.9 %, followed by Bacillus cereus (50.3 %), Bacillus subtilis (44 %), Pseudomonas aeruginosa (37.9 %) and the least was seen in Micrococcus spp (35.3 %). These findings revealed that some bacteria species are capable of utilizing the oil and used it as sole source of carbon and energy and the mixed consortia of the bacteria have rapid degradation ability. Biological degradation of hydrocarbon contaminated soil offers a better and more environmentally friendly technique that if properly explored can bring our environment into a better place for both plant and animal.

scholarly journals RHIZODEGRADATION OF PHENANTHRENE, ANTHRACENE AND PYRENE BY AUGMENTING BACILLUS CEREUS AND BACILLUS SUBTILIS STRAINS

2021 ◽  
Vol 9 (02) ◽  
pp. 89-96
Author(s):  
M. Poornachander Rao ◽  
◽  
Anitha Yerra ◽  
K. Satyaprasad ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Contaminated Soils ◽  
Rhizosphere Soil ◽  
Culture Method ◽  
Experimental Period ◽  
Bacterial Strains ◽  
Bacterial Populations ◽  
Metabolic Activities ◽  
Pot Culture

Rhizodegradation is one of the best methods for the effective removal of dangerous polycyclic aromatic hydrocarbons pollutants from soil. This is operative due to the high persistent, non-bioavailability nature of PAHs and combined, sequential reactions of bacteria present in rhizosphere of plants. We have conducted pot-culture method to study the degradation of three PAHs compounds namely phenanthrene, anthracene and pyrene in artificially contaminated soils of rhizosphere and non-rhizosphere soil treatments of blackgram(Vignamungo L.) that augmented by two potential PAHs degraders namely Bacillus cereus CPOU13 and Bacillus subtilis SPC14 isolated from naturally contaminated soils for 90days. HPLC studies revealed that degradation percentages of the three PAHs in treatments were more where selected strains augmented to the soil treatments over the non-augmented soils. The rhizosphere treatments that have augmented strains recorded more degradation percentages of phenanthrene, anthracene and pyrene over the rhizosphere treatments that were non-augmented. Pyrene, a high molecular weight PAHs degraded maximum to 96.24% in rhizosphere soil treatment that is augmented with the strains while moderate degradation of pyrene recorded in non-autoclaved soil treatments that contain natural microbial communities. The study of counting of bacterial populations during the experimental period revealed that the populations of the selected and other natural bacteria were gradually increased from the first day, reached maximum by 60days and became almost consistent in 90days in all the treatments. It was also observed that the populations of bacteria were high in rhizosphere treatments compared to the non-rhizosphere soil treatments. With these results it has been predicted that degradation of PAHs in rhizosphere soil treatments is closely associated with the increasing PAHs degrading bacterial populations of selected bacterial strains that may consume more quantity of PAHs for their metabolic activities in rhizosphere soils. Key words: Rhizodegradation, PAHs, HPLC, pot culture.

scholarly journals Wastewater Collection and Transportation through Drainage Network- a Potential Threat to Biodiversity & Local Eco-system in Zanzibar Stone-town & Surroundings

2018 ◽  
pp. 1-13
Author(s):  
Z. M. Abubakar ◽  
A. R. Rabia ◽  
A. M. Ussi ◽  
M. A. Bakar ◽  
R. A. Ali
Keyword(s):  
Waste Water ◽  
Urban Areas ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Bacterial Load ◽  
Waste Water Treatment ◽  
Potential Threat ◽  
Cross Sectional ◽  
Health Crisis ◽  
High Turbidity

Waste water effluents voided in the coastal areas of Zanzibar are highly polluted posing a public health crisis. This cross-sectional study, carried out in September 2017, investigated bacterial load and physicochemical characteristics of the effluents discharged into coastal marine waters of the Zanzibar stone town and peri-urban areas. Ten drains, seven of which were impervious and three pervious, were sampled twice per day in mornings and afternoons. Each drain was sampled at three sites; the out-pour site close to the sea and two sites upstream 100 meters apart. Physicochemical parameters including Dissolved Oxygen (DO), pH and turbidity were determined immediately after arrival in the laboratory. Colony forming units/ ml were also determined.  Bacterial counts were higher in impervious drains, in out-pour sites and in the afternoons. Conversely counts were lower in the out-pour sites compared to upstream sites in the pervious drains. DO was significantly higher in the pervious compared to impervious drains (p = 0.004). Turbidity was high in the mornings, but one drain had significantly high turbidity (p=0.0009). In all drains pH was high in the afternoon than mornings. Thirteen bacterial species, mostly gram-negative Enterobacteriaceae were isolated. Vibrio cholerae was isolated from 5 drains. Ground water close to pervious drains had coliforms beyond WHO guidelines. Waste water drains in Zanzibar urban and peri-urban areas are vastly contaminated with organic matter and pathogenic bacteria leading to high turbidity and oxygen depletion. It is recommended to increase awareness to the public and introduce waste water treatment strategies both physically and chemically.

scholarly journals Isolation of bacteria from diesel contaminated soil for diesel remediation

2019 ◽  
Vol 28 ◽  
pp. 33-41 ◽  
Author(s):  
OA Oyewole ◽  
SS Leh Togi Zobeashia ◽  
OE Oladoja ◽  
IO Musa ◽  
IT Terhemba
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Large Scale ◽  
Bacterial Species ◽  
Plastic Container ◽  
Hydrocarbon Concentration ◽  
Optimum Ph

This study is aimed at isolating bacterial species that inhabit diesel contaminated soil and also screened these isolates for the ability to be used for remediating diesel contaminated environment using their potential to degrade diesel as carbon and energy source. Top soil sample was collected from an ancient diesel-powered generator house in Minna, Nigeria, in a sterilized plastic container while diesel oil was obtained from local petrol bunk. Four bacterial isolates were isolated from the diesel contaminated soil sample and were screened for their ability to degrade diesel using mineral salt medium (MSM). The isolates with highest biodegradation potential were identified as Bacillus subtilis and Bacillus cereus. The optimum pH (5, 6, 7 and 8) and hydrocarbon concentration (1%, 2%, 5% and 10%) of the isolate was determined by spectrophotometry and the result revealed that the optimum pH for biodegradation of diesel by Bacillus subtilis and Bacillus cereus, was 7 (1.170) and 8 (1.745) respectively while the optimum hydrocarbon concentration degradation for both isolates was 5% (2.22) and 1% (2.37) respectively. The results of this study showed that these isolates were able to degrade diesel and can be useful for large scale bioremediation of diesel contaminated soils. J. bio-sci. 28: 33-41, 2020

scholarly journals Isolation and Screening of Water Microbes for Decolourisation of Textile Dye Waste

2016 ◽  
Vol 11 (1) ◽  
pp. 296-300
Author(s):  
J. K Singh ◽  
R Ranjan ◽  
Pranay Pankaj
Keyword(s):  
Waste Water ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Azo Dyes ◽  
Bacterial Species ◽  
Textile Dye ◽  
Human Beings ◽  
Degrading Bacteria ◽  
Vis Spectroscopy ◽  
Pre Treatment

Azo dyes are widely used in textile industry. Unused dyes, consisting mainly non biodegradable released along with waste water streams without any proper pre-treatment which cause nuisance for environment and accumulate in flora as well as fauna. These also exhibit allergic, carcinogenic and mutagenic properties for human beings. Isolation and screening of azo dye degrading bacteria are economic in biodegradation and detoxification. In the present study, 200 waste water samples were collected from dye-contaminated sites of textile industries and bacterial species such as Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida were isolated and identified. Evaluation of decolorizing properties of these bacteriae were done by UV-Vis spectroscopy (Amax 596 nm) in different concentrations using different carbon sources such as Hans’s medium and GYP medium. Maximum decolourisation of 0.1% azo dyes were recorded to be 89.0%, 91% and 86% in Hans medium containing charcoal source by Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida respectively at 24 hrs. These bacterial isolates may be utilized in large scale for pre-treatment for ecological balance by avoiding water pollution.

scholarly journals Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia

2021 ◽  
Vol 9 (10) ◽  
pp. 2109
Author(s):  
Diogo Alexandrino ◽  
Ana Mucha ◽  
Maria Paola Tomasino ◽  
C. Marisa R. Almeida ◽  
Maria Carvalho
Keyword(s):  
Agricultural Soil ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
The Other ◽  
Microbial Consortia ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
First Time ◽  
Culture Dependent

Epoxiconazole (EPO) and fludioxonil (FLU) are two widely used fluorinated pesticides known to be highly persistent and with high ecotoxicological potential, turning them into pollutants of concern. This work aimed to optimize two degrading bacterial consortia, previously obtained from an agricultural soil through enrichment with EPO and FLU, by characterizing the contribution of their corresponding bacterial isolates to the biodegradation of these pesticides using both culture-dependent and independent methodologies. Results showed that a co-culture of the strains Hydrogenophaga eletricum 5AE and Methylobacillus sp. 8AE was the most efficient in biodegrading EPO, being able to defluorinate ca. 80% of this pesticide in 28 days. This catabolic performance is likely the result of a commensalistic cooperation, in which H. eletricum may be the defluorinating strain and Methylobacillus sp. may assume an accessory, yet pivotal, catabolic role. Furthermore, 16S rRNA metabarcoding analysis revealed that these strains represent a minority in their original consortium, showing that the biodegradation of EPO can be driven by less abundant phylotypes in the community. On the other hand, none of the tested combinations of bacterial strains showed potential to biodegrade FLU, indicating that the key degrading strains were not successfully isolated from the original enrichment culture. Overall, this work shows, for the first time, the direct involvement of two bacterial species, namely H. eletricum and Methylobacillus sp., in the biodegradation of EPO, while also offering insight on how they might cooperate to accomplish this process. Moreover, the importance of adequate culture-dependent approaches in the engineering of microbial consortia for bioremediation purposes is also emphasized.

scholarly journals Cadmium and lead tolerant bacteria isolated from industrial waste water

2017 ◽  
Vol 26 (1) ◽  
pp. 29-38
Author(s):  
Md Arifur Rahman Bhuiyan ◽  
Tazeen Fatima Khan ◽  
Shahjahan Choudhury ◽  
SM Imamul Huq
Keyword(s):  
Waste Water ◽  
Heavy Metals ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Industrial Waste ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Industrial Waste Water ◽  
Acinetobacter Baumanii ◽  
Cadmium And Lead

An in vitro study was conducted to isolate, identify and characterize heavy metal resistant bacteria from industrial waste water and to determine their tolerance capacity to cadmium and lead. Different morphological, physiological and biochemical tests were carried out to identify the bacterial isolates in the waste water. A total of eight bacterial isolates viz., Staphyllococcus intermedius, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Escherichia coli, Acinetobacter baumanii, Pseudomonas flavescens and Acinetobacter lwofii were identified from metal polluted tannery and steel industrial areas. Bacterial response to cadmium tolerance was determined by treating them with CdCl2 solution at a rate of 1, 2, 2.5 and 5 μg/ml. In case of lead tolerant bacteria, PbCl2 solution was applied at a rate of 0.05, 0.125, 0.2 and 0.5 μg/ml. Pseudomonas aeruginosa isolated from waste water of steel industries and Bacillus cereus from tannery area was found to be the most tolerant species to the different doses of cadmium and lead. The study indicated that Pseudomonas aeruginosa and Bacillus cereus could be good candidates for the treatment and elimination of heavy metals from industrial waste water. The present study may be helpful to the bioremediation of heavy metals in the contaminated environment. Dhaka Univ. J. Biol. Sci. 26(1): 29-38, 2017 (January)

scholarly journals Study the antibiotics sensitivity and beta- lactamase productivity of some Staphylococcus spp. Isolates from different sources of the Al Jamhoree Teaching Hospital in Mosul City

2019 ◽  
Vol 24 (7) ◽  
pp. 27
Author(s):  
Ayman Mohammed Khaleel1 ◽  
Adeeba Younis Shareef ◽  
, Shakeeba Younis Shareef
Keyword(s):  
Staphylococcus Aureus ◽  
Teaching Hospital ◽  
Clinical Laboratory ◽  
Bacterial Species ◽  
Age Groups ◽  
National Committee ◽  
Beta Lactamase ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Staphylococcus Spp

The study includes the isolation of certain types of Gram-positive bacteria Staphylococcus spp..The sample materials (blood, wounds, burns) were collected from both genders of all age groups of inpatients in Al Jamhoree Teaching Hospital in the Mosul during June till end of November 2018. Staphylococcus aureus was the most common among the isolates with 7 isolates(38.9%) followed by Staphylococcus epidermidis, Staphylococcus scriuri Staphylococcus lentus (Staphylococcus simulans) with 2 isolates each with 11.1% followed by Staphylococcus chromogenase, Staphylococcu scapilis and  Staphylococcus xylosus with one isolate for each one 5.5%. The number of isolates was 9 isolates and 50%, followed by wound samples. The isolates were 6 isolated by 33% and the samples of burns were 3 isolates. 17% were isolated and Staphylococcus aureus was the most dominant species. The sensitivity of bacterial isolates was  studied for 15 antibiotics for different antibiotic groups. The results showed that a difference in the resistance ratio of the isolates to these antagonists, as they were resistant to the Ceftrixone, Cloxacillin 100%. The Ciprofloxacillin, Gentamycin, Pipracillin, and Amikacin resistance were  reduced. The Ciprofloxacillin antagonist was the most affected on the bacterial isolates studied, followed by the Rifampin. The results showed that the beta-lactamase enzyme was not produced by any of the Iodic methods by 4 bacterial strains, while the rest of the species varied in the susceptibility of production to the enzyme. The Iodine tube is one of the best methods to detect the production of these enzymes. The susceptibility of bacterial species to the production of large-spectrum beta-lactase enzymes was also tested using the National Committee for Clinical Laboratory Standards (NCCL) and the double-disc method. Staphylococcus lentus, Staphylococcus capilis, Staphylococcus chromanogenes The first NCCL has shown a single isolation of Staphylococcus capilis ability to produce it.   http://dx.doi.org/10.25130/tjps.24.2019.125

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