AN ASSESSMENTOF PHTHALATES IN COSMETIC PRODUCTS BY DISPERSIVE LIQUID-LIQUID EXTRACTION METHOD USING HPLCAND LC-MS/MS

A Dispersive liquid-liquid microextraction (DLLME) based analytical method was developed and validated using HPLC-PDA and LC-MS/MS for quantitative determination of phthalates (Dimethyl phthalate (DMP), Diethyl phthalate (DEP), Benzyl Butyl Phthalate (BzBP), Dibutylphthalate(DBP), Diethylhexyl phthalate(DEHP), Di-n-octyl phthalate (DOP) in different cosmetic products (After Shave Lotion, Deodorants, Perfume and Liquid Body Lotion). The DLLME based developed and validated analytical method was found specific, sensitive, accurate and precise. The accuracy (% recovery) of the method at a spiking level of 0.1, 0.5 and 1.0 mg L-1 in the different cosmetic product was found in the range of 92-108. The interday and intraday precision (%RSD) of the method was found less than 15. Out of six analyzed phthalates, only four phthalates were detected in different cosmetic products. Dimethyl phthalate (DMP) and Di-n-octyl phthalate (DOP) were detected in aftershave lotion. Diethyl phthalate (DEP) was detected in deodorants. Dibutyl phthalate (DBP) and Diethyl phthalate (DEP) were detected in perfumes. None of the phthalates were detected in liquid body lotion.

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Determination of Selected Phthalates in Some Commercial Cosmetic Products by HPLC-UV

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207323666200630113850 ◽

2020 ◽

Vol 23 (10) ◽

pp. 1010-1022

Author(s):

Emrah Dural

Keyword(s):

High Performance ◽

Phthalate Esters ◽

High Sensitivity ◽

Hplc Method ◽

Cosmetic Products ◽

Limit Of Quantification ◽

Nail Polish ◽

Accuracy And Precision ◽

Butyl Phthalate

Aim and scope: Due to the serious toxicological risks and their widespread use, quantitative determination of phthalates in cosmetic products have importance for public health. The aim of this study was to develop a validated simple, rapid and reliable high-performance liquid chromatography (HPLC) method for the determination of phthalates which are; dimethyl phthalate (DMP), diethyl phthalate (DEP), benzyl butyl phthalate (BBP), di-n-butyl phthalate (DBP), di(2- ethylhexyl) phthalate (DEHP), in cosmetic products and to investigate these phthalate (PHT) levels in 48 cosmetic products marketing in Sivas, Turkey. Materials and Methods: Separation was achieved by a reverse-phase ACE-5 C18 column (4.6 x 250 mm, 5.0 μm). As the mobile phase, 5 mM KH2PO4 and acetonitrile were used gradiently at 1.5 ml min-1. All PHT esters were detected at 230 nm and the run time was taking 21 minutes. Results: This method showed the high sensitivity value the limit of quantification (LOQ) values for which are below 0.64 μg mL-1 of all phthalates. Method linearity was ≥0.999 (r2). Accuracy and precision values of all phthalates were calculated between (-6.5) and 6.6 (RE%) and ≤6.2 (RSD%), respectively. Average recovery was between 94.8% and 99.6%. Forty-eight samples used for both babies and adults were successfully analyzed by the developed method. Results have shown that, DMP (340.7 μg mL-1 ±323.7), DEP (1852.1 μg mL-1 ± 2192.0), and DBP (691.3 μg mL-1 ± 1378.5) were used highly in nail polish, fragrance and cream products, respectively. Conclusion: Phthalate esters, which are mostly detected in the content of fragrance, cream and nail polish products and our research in general, are DEP (1852.1 μg mL-1 ± 2192.0), DBP (691.3 μg mL-1 ± 1378.5) and DMP (340.7 μg mL-1 ±323.7), respectively. Phthalates were found in the content of all 48 cosmetic products examined, and the most detected phthalates in general average were DEP (581.7 μg mL-1 + 1405.2) with a rate of 79.2%. The unexpectedly high phthalate content in the examined cosmetic products revealed a great risk of these products on human health. The developed method is a simple, sensitive, reliable and economical alternative for the determination of phthalates in the content of cosmetic products, it can be used to identify phthalate esters in different products after some modifications.

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Biodegradation of Endocrine Disrupting Chemicals Benzyl Butyl Phthalate and Dimethyl Phthalate by Bacillus marisflavi RR014

Journal of Applied Microbiology ◽

10.1111/jam.15045 ◽

2021 ◽

Author(s):

Rajanbir Kaur ◽

Arpna Kumari ◽

Gauri Sharma ◽

Drishtant Singh ◽

Rajinder Kaur

Keyword(s):

Endocrine Disrupting Chemicals ◽

Dimethyl Phthalate ◽

Benzyl Butyl Phthalate ◽

Endocrine Disrupting ◽

Butyl Phthalate

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Validation of analytical method for the quantitative determination of preservative acids allowed in Eco label cosmetic products

MOJ Toxicology ◽

10.15406/mojt.2018.04.00117 ◽

2018 ◽

Vol 4 (4) ◽

Author(s):

Stephane Pirnay

Keyword(s):

Quantitative Determination ◽

Analytical Method ◽

Cosmetic Products ◽

Validation Of Analytical Method

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Evaluation of method for phthalate extraction from milk related to milk dilution ratio

Journal of the Serbian Chemical Society ◽

10.2298/jsc141204028m ◽

2015 ◽

Vol 80 (8) ◽

pp. 983-996 ◽

Cited By ~ 1

Author(s):

Danica Milojkovic ◽

Darko Andjelkovic ◽

Gordana Kocic ◽

Tatjana Andjelkovic

Keyword(s):

Salt Effect ◽

Gas Chromatography Mass Spectrometry ◽

Extraction Techniques ◽

Selected Ion Monitoring ◽

Benzyl Butyl Phthalate ◽

Liquid Liquid Extraction ◽

Solvent Type ◽

Butyl Phthalate ◽

Ethylhexyl Phthalate

Liquid-liquid extraction techniques were compared coupled with gas chromatography-mass spectrometry (GC-MS), for the extraction and the determination of four phthalates: dimethyl phthalate (DMP), di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP) and di-(2-ethylhexyl) phthalate (DEHP) in six different kinds of milk-based samples. Extraction factors: sample preparation, organic solvent type and volume, salt effect, agitation and the extraction time were optimized. The ion of base peaks (m/z 149 for DBP, BBP and DEHP and m/z 163 for DMP) for investigated phthalates were selected for the screening studies. The acquisition was performed at the selected ion monitoring mode. The MSD response for GC-MS phthalate calibration standards was linear between 0.25 and 2.50 ?g mL-1 with calculated LODs between 0.01 ?g mL-1 to 0.04 ?g mL-1 and LOQs of 0.05 ?g mL-1 to 0.12 ?g mL-1, while repeatability was between 1.7 % to 4.9 % RSD. The study demonstrated an increase of the recovery of less polar phthalates in matrix milk standards by matrix dilution. Recovery for hydrophilic phthalates, like DMP, was not changed by matrix dilution and it was continuously low for the investigated method. Two spiking levels were tested for influence of matrix dilution on phthalate recovery, showed the same trend.

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