scholarly journals PHYTOCHEMICAL STUDY AND ANTIBACTERIAL ACTIVITY OF ETHANOLIC AND AQUEOUS EXTRACTS OF BARKS OF MYRIANTHUS HOLSTII ENGL. (CECROPIACEAE)

2021 ◽  
Vol 9 (11) ◽  
pp. 1143-1150
Author(s):  
Yeo Sounta Oumar ◽  
◽  
Monyn Ebalah Delphine ◽  
Silue Kalamourou ◽  
Mawa Traore ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Extraction Yield ◽  
Bacterial Strains ◽  
Phytochemical Study ◽  
Chemical Screening ◽  
E Coli ◽  
Variable Sensitivity ◽  
Dose Dependent

The aim of this work is to study phytochemicals and evaluate the antibacterial activity of aqueous and ethanolic extracts of Myrianthus holstii bark on three reference and five clinical strains derived from biological products. The results obtained show that the gives the best extraction yield. Chemical screening revealed the presence of polyphenols, alkaloids, flavonoids, saponosides, quinones, anthocyanins, tannins, terpenoids and sterols in both extracts. The results obtained show that the strains tested have a variable sensitivity for the two extracts and their concentrations. Diffusion and dilution methods on Muller-Hinton were used to evaluate the antibacterial activity of the extracts. The diameters of the inhibition zones are between 8 and 16 mm for the ethanolic extract starting from 25 mg/mL and between 8 and 12 mm for the aqueous extract at 50 mg/mL. The results revealed that these extracts have a dose-dependent antibacterial activity on the bacterial strains used. However, the 70% ethanolic extract has a better antibacterial potential on the strains compared with the aqueous extract, namely on S. aureus (CMI=3.12 mg/mL), E. coli ATCC (CMI=12.5 mg/mL) and S. aureus Meti-R (CMI=12.5 mg/mL). Also, this extract is bactericidal on all strains studied and its MIC ranges from 3.12 to 100 mg/mL after 24 and 48 hours of incubation. This study showed that extracts of Myrianthus holstii could be used in the treatment of infectious diseases.

scholarly journals Phytochemical screening and synergistic interactions between aminoglycosides, selected antibiotics and extracts from the bryophyte Octoblepharum albidum Hedw (Calymperaceae)

2012 ◽  
Vol 64 (2) ◽  
pp. 465-470 ◽  
Author(s):  
C.A.S. Vidal ◽  
E.O. Sousa ◽  
Fabíola Rodrigues ◽  
Adriana Campos ◽  
S.R. Lacerda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Synergistic Effect ◽  
Ethanolic Extract ◽  
Antibiotic Activity ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
E Coli ◽  
Synergistic Interactions

This work is the first to describe the modulation of antibiotic activity of the bryophyte Octoblepharum albidum Hedw extract. The antibacterial activity of ethanolic extract of O. albidum (EEOa), alone and in association with aminoglycosides, was determined against six bacterial strains by a microdilution test. The results showed a similar inhibitory activity of EEOa against Escherichia coli ATCC 25922 and Klebsiella pneumoniae ATCC 33018 (MICs 512 ?g/mL). The synergistic effect of the extracts and aminoglycosides was also verified. The most pronounced effects were obtained with EEOa + gentamicin against E. coli and EEOa + kanamycin against K. pneumoniae with MICs reduction (128 to 32 ?g/mL). The data from this study are indicative of the antibacterial activity of the bryophyte O. albidum extracts and its potential in modifying the resistance of aminoglycosides analyzed.

scholarly journals Investigation of the Phyto-constituents and the Antibacterial Activity of Burkea africana against P. aeruginosa, E. coli, S. aureus and E. faecalis

2021 ◽  
pp. 33-40
Author(s):  
S. Zengeni ◽  
J. Chifamba ◽  
I. Mutingwende ◽  
T. Manyarara
Keyword(s):  
Antibacterial Activity ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Antibacterial Efficacy ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
E Coli

Bacterial skin infections are common in the tropics and the emergence of resistant bacterial strains has complicated treatment outcomes especially in immune compromised patients. This study focused on determining the antibacterial activity and preliminary phytochemical screening of the plant, Burkea Africana, which has been used for decades to treat various ailments in Southern Africa. Phyto-constituent determination and antibacterial assay was carried out on the hydro-ethanolic and aqueous leaf extracts of Burkea Africana. The Agar well diffusion method was used to investigate the antibacterial efficacy of both extracts. Activity was measured against P. aeruginosa, E. coli, S. aureus and E. faecalis using ciprofloxacin as the standard. For both extracts, phytochemical analysis revealed the presence of steroids, tannins, saponins and carbohydrates. The 70% hydro-ethanolic extract however revealed more phyto-constituents including flavonoids and alkaloids. For both extracts antibacterial activity was comparable to the standard. The highest zone of inhibition recorded for the water extract was 22±0.4 mm at 500 mg/ml for E. coli while the standard, Ciprofloxacin had a zone of inhibition of 25±0.33 mm. The ethanolic extract showed higher antibacterial efficacy when compared to ciprofloxacin against S. aureus. The zone of inhibition for the 70% ethanolic extract was 25.4±0.50 mm and that of the standard was 27±0.48 mm. Antibacterial activity was generally higher in the ethanolic extract than the water extract. It is therefore concluded that B. africana exhibits antibacterial activity.

scholarly journals Antibacterial Activity of Lyophilized Aqueous Extract of Coriaria Myrtifolia from Northern Morocco

2013 ◽  
Vol 3 (3) ◽  
pp. 280-285
Author(s):  
Karima Hezbri ◽  
Maha Hafse ◽  
Abdellah Farah ◽  
Imed Sbissi ◽  
Maher Gtari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Aqueous Extract ◽  
The Other ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Streptomyces Sp ◽  
E Coli ◽  
Other Hand

This work aims to evaluate the antibacterial activity of lyophilized aqueous extract of Coriaria myrtifolia against twentythree bacterial strains belonging to twelve main genera, known to be involved in some pathologies and / or in the foodspoilage process: Bacillus sp., E. coli, E. hafnia, Enterococcus sp., Klebsiella sp,. Listeria monocytogenes, Pseudomonassp., Salmonella sp., Serratia sp., Shigella sp., S. aureus and Streptomyces sp. To achieve this goal, the disk diffusionmethod was used.The study revealed that C. myrtifolia’s aqueous extract is active against most of the tested strains and that activity isproportional to its concentration. The intensity of inhibition depends on the extract’s concentration on one hand and on thebacterial species tested on the other hand.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

scholarly journals Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects

Antibiotics ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 98 ◽  
Author(s):  
Eunice Mgbeahuruike ◽  
Pia Fyhrquist ◽  
Heikki Vuorela ◽  
Riitta Julkunen-Tiitto ◽  
Yvonne Holm
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Hot Water ◽  
Bacterial Strains ◽  
Inhibitory Effects ◽  
Piper Guineense ◽  
E Coli ◽  
Growth Inhibitory ◽  
Derivatives Of

Piper guineense is a food and medicinal plant commonly used to treat infectious diseases in West-African traditional medicine. In a bid to identify new antibacterial compounds due to bacterial resistance to antibiotics, twelve extracts of P. guineense fruits and leaves, obtained by sequential extraction, as well as the piperine and piperlongumine commercial compounds were evaluated for antibacterial activity against human pathogenic bacteria. HPLC-DAD and UHPLC/Q-TOF MS analysis were conducted to characterize and identify the compounds present in the extracts with promising antibacterial activity. The extracts, with the exception of the hot water decoctions and macerations, contained piperamide alkaloids as their main constituents. Piperine, dihydropiperine, piperylin, dihydropiperylin or piperlonguminine, dihydropiperlonguminine, wisanine, dihydrowisanine and derivatives of piperine and piperidine were identified in a hexane extract of the leaf. In addition, some new piperamide alkaloids were identified, such as a piperine and a piperidine alkaloid derivative and two unknown piperamide alkaloids. To the best of our knowledge, there are no piperamides reported in the literature with similar UVλ absorption maxima and masses. A piperamide alkaloid-rich hexane leaf extract recorded the lowest MIC of 19 µg/mL against Sarcina sp. and gave promising growth inhibitory effects against S. aureus and E. aerogenes as well, inhibiting the growth of both bacteria with a MIC of 78 µg/mL. Moreover, this is the first report of the antibacterial activity of P. guineense extracts against Sarcina sp. and E. aerogenes. Marked growth inhibition was also obtained for chloroform extracts of the leaves and fruits against P. aeruginosa with a MIC value of 78 µg/mL. Piperine and piperlongumine were active against E. aerogenes, S. aureus, E. coli, S. enterica, P. mirabilis and B. cereus with MIC values ranging from 39–1250 µg/mL. Notably, the water extracts, which were almost devoid of piperamide alkaloids, were not active against the bacterial strains. Our results demonstrate that P. guineense contains antibacterial alkaloids that could be relevant for the discovery of new natural antibiotics.

scholarly journals Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of Punica granatum (Pomegranate) Leaves

Scientifica ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Amine Trabelsi ◽  
Mohamed Amine El Kaibi ◽  
Aïmen Abbassi ◽  
Amira Horchani ◽  
Leila Chekir-Ghedira ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Phytochemical Study ◽  
E Coli ◽  
Microdilution Method ◽  
Resistant Strains

This study aimed to determine phytochemical contents, antibacterial properties, and antibiotic modulating potential of Punica granatum leaf extracts: hexane, chloroform, ethyl acetate, ethanol, and aqueous extracts as well as an extract enriched with total oligomer flavonoids (TOFs). The TOF extract contained the highest value of phenols and flavonoids. Rutin, luteolin, gallic acid, and ellagic acid were determined by HPLC analysis of this extract. The antibacterial activity was assayed by the disc diffusion method and microdilution method against Staphylococcus aureus and Escherichia coli standard ATCC strains and clinical isolates resistant strains. The TOF extract was the most active against all tested strains. The checkerboard method was used for the determination of synergy between two antibiotics (amoxicillin and cefotaxime) and P. granatum leaf extracts. The best synergistic interaction was found with TOF extract combined with amoxicillin for penicillin-resistant E. coli and penicillin-resistant S. aureus. These results can be assigned to tannins, flavonoids, and phenolic acids found in P. granatum leaf extracts. Pomegranate leaf extracts or active compounds isolated from these extracts could be used to fight the emergence and spread of resistant bacterial strains.

scholarly journals Methylkarranjic acid and Pongamol from Derris indica Seeds and their Antibacterial Activity

1970 ◽  
Vol 6 (1) ◽  
pp. 9-13 ◽  
Author(s):  
M Abdullahil Baki ◽  
Golam Sadik ◽  
KAM Shahadat Hossain Mondal ◽  
M Ashilk Mosaddik ◽  
M Mukhlesur Rahman
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Soluble Fraction ◽  
Ethanolic Extract ◽  
2D Nmr ◽  
Shigella Dysenteriae ◽  
E Coli ◽  
Nmr Data ◽  
Test Organisms ◽  
Derris Indica

Two phenolic compounds, methylkarranjic (1) acid and pongamol (2), were isolated from the petroleum ether soluble fraction of an ethanolic extract of Derris indica seeds. The structures of these compounds were confirmed by LC-MS and a series of 1D and 2D NMR data. The solvent solvent partionates of the ethanolic extract and compounds (1 and 2) exhibited moderate antibacterial activity against several test organisms. The minimum inhibitory concentrations (MICs) of 1 and 2 were found to be in the range of 32-128 μg/ml against Bacillus megatorium, Streptococcus β-haemolyticus, Shigella dysenteriae and E. coli. Key words: Derris indica; Leguminosae; Methylkarranjic acid; Pongamol; Antibacterial activity Dhaka Univ. J. Pharm. Sci. 6(1): 9-13, 2007 (June) The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Design, Synthesis and Antibacterial Activity of Coumarin-1,2,3-triazole Hybrids Obtained from Natural Furocoumarin Peucedanin

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2126 ◽  
Author(s):  
Alla V. Lipeeva ◽  
Danila O. Zakharov ◽  
Liubov G. Burova ◽  
Tatyana S. Frolova ◽  
Dmitry S. Baev ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Cycloaddition Reaction ◽  
Bacterial Strains ◽  
Design Synthesis ◽  
Docking Simulations ◽  
E Coli ◽  
Antibiotic Drugs ◽  
In Vitro Antibacterial Activity ◽  
Substituted Coumarins

Synthesis of 1,2,3-triazole-substituted coumarins and also 1,2,3-triazolyl or 1,2,3-triazolylalk-1-inyl-linked coumarin-2,3-furocoumarin hybrids was performed by employing the cross-coupling and copper catalyzed azide-alkyne cycloaddition reaction approaches. The synthesized compounds were evaluated for their in vitro antibacterial activity against Staphylococcus aureus, Bacillius subtilis, Actinomyces viscosus and Escherichia coli bacterial strains. Coumarin-benzoic acid hybrids 4с, 42с and 3-((4-acetylamino-3-(methoxycarbonyl)phenyl)ethynyl)coumarin (29) showed promising activity against S. aureus strains, and the 1,2,3-triazolyloct-1-inyl linked coumarin-2,3-furocoumarin hybrid 37c was endowed with high selectivity against B. subtilis and E. coli species. The in vitro antibacterial activity of 4с, 29, 37c and 42с can potentially be compared with that of a number of modern antibiotic drugs used in the clinic, suggesting promising prospects for further research. A detailed study of the molecular interactions with the targeted protein MurB was performed using docking simulations and the obtained results are quite promising.

scholarly journals Antibacterial Activity of Tannic Acid and Tannic Acid/Amphiphilic Cationic Polymer Mixtures

2020 ◽  
Vol 32 (6) ◽  
pp. 1491-1496
Author(s):  
Fatimah M. Alzahrani ◽  
Stephen G. Yeates ◽  
Michelle Webb ◽  
Hind Ali Alghamdi
Keyword(s):  
Antibacterial Activity ◽  
Tannic Acid ◽  
Cationic Polymer ◽  
Polymer Mixture ◽  
Klebsiella Pneumonia ◽  
Cationic Polymers ◽  
Polymer Mixtures ◽  
Bacterial Strains ◽  
Gram Positive ◽  
E Coli

In this study, the antibacterial activity of tannic acid/amphiphilic cationic polymer (poly{2-[(methacryloyloxy)ethyl]trimethyl-ammonium chloride}, PMADQUAT) and tannic acid mixtures was examined on the strains of Gram-positive (S. aureus) and Gram-negative (E. coli CI2, E. coli K12, Klebsiella pneumonia and P. aeruginosa) bacteria. Tannic acid exhibited the antibacterial activity against all the studied bacterial strains. The ester linkage between glucose and gallic acid is vital for the antimicrobial activity of tannic acid. Tannic acid inhibited the growth of S. aureus and E. coli K12 (1 wt%) and reduced the growth of P. aeruginosa to 23%. Mixing cationic polymers having different structures (statistical copolymer, homopolymer and diblock polymer) with tannic acid lead to an increase in antibacterial activity of tannic acid and the stability and clarity of mixtures was higher than that of a pure tannic acid solution. Tannic acid/diblock polymer and tannic acid/homopolymer mixtures (0.1 wt%) were excellent for inhibiting the growth of planktonic E. coli K12 bacteria, and a low concentration (0.0001 wt%) of tannic acid/diblock polymer reduced its growth to 19%. By contrast, the tannic acid/statistical polymer mixture (0.0001 wt%) was excellent for inhibiting the growth of Gram-positive S. aureus bacteria.

scholarly journals Photon-Induced Superior Antibacterial Activity of Palladium-Decorated, Magnetically Separable Fe3O4/Pd/mpg-C3N4 Nanocomposites

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3888 ◽  
Author(s):  
Deepika Thakur ◽  
Qui Thanh Hoai Ta ◽  
Jin-Seo Noh
Keyword(s):  
Antibacterial Activity ◽  
Solution Method ◽  
Pd Nanoparticles ◽  
Bacterial Degradation ◽  
Photoluminescence Intensity ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
E Coli ◽  
Carrier Recombination ◽  
Solar Light Irradiation

Three-component nanocomposites (Fe3O4/Pd/mpg-C3N4) have been systematically synthesized using a three-step solution method for the photocatalytic bacterial decontamination. The mesoporous g-C3N4 nanosheets (mpg-C3N4), which were prepared by the acid treatment, showed a great improvement in photocatalytic performance. The photoluminescence intensity of the mpg-C3N4 nanosheets was disclosed to drop about 60% from the value of normal g-C3N4 nanosheets. Decoration of mpg-C3N4 with palladium (Pd) nanoparticles led to the effective suppression of carrier recombination and the carrier migration to Fe3O4 nanoparticles. It was revealed that the three-component nanocomposites degraded 99.9% of E. coli and 99.8% of S. aureus bacterial strains within 2 h of solar light irradiation at a 100 μg/mL concentration, demonstrating their superb photocatalytic antibacterial activity. In addition, the nanocomposites could be easily separated from the bacterial cells and repeatedly used for photocatalytic bacterial degradation with good recyclability. The strong photon-induced antibacterial activity and good recyclability of the three-component nanocomposites may represent their potential as a promising antibacterial photocatalyst.

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