scholarly journals Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of Punica granatum (Pomegranate) Leaves

Scientifica ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Amine Trabelsi ◽  
Mohamed Amine El Kaibi ◽  
Aïmen Abbassi ◽  
Amira Horchani ◽  
Leila Chekir-Ghedira ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Phytochemical Study ◽  
E Coli ◽  
Microdilution Method ◽  
Resistant Strains

This study aimed to determine phytochemical contents, antibacterial properties, and antibiotic modulating potential of Punica granatum leaf extracts: hexane, chloroform, ethyl acetate, ethanol, and aqueous extracts as well as an extract enriched with total oligomer flavonoids (TOFs). The TOF extract contained the highest value of phenols and flavonoids. Rutin, luteolin, gallic acid, and ellagic acid were determined by HPLC analysis of this extract. The antibacterial activity was assayed by the disc diffusion method and microdilution method against Staphylococcus aureus and Escherichia coli standard ATCC strains and clinical isolates resistant strains. The TOF extract was the most active against all tested strains. The checkerboard method was used for the determination of synergy between two antibiotics (amoxicillin and cefotaxime) and P. granatum leaf extracts. The best synergistic interaction was found with TOF extract combined with amoxicillin for penicillin-resistant E. coli and penicillin-resistant S. aureus. These results can be assigned to tannins, flavonoids, and phenolic acids found in P. granatum leaf extracts. Pomegranate leaf extracts or active compounds isolated from these extracts could be used to fight the emergence and spread of resistant bacterial strains.

scholarly journals Morphology, microscopic anatomy and bacterial inhibition of extracts of Ludwigia adscendens (L.) H. Hara plants growing in Lang Sen Wetland Reserve, Long An province, Vietnam

2021 ◽  
Vol 17 (3) ◽  
pp. 160-170
Author(s):  
Van Tien Dung ◽  
Huynh Nguyen Van Anh ◽  
Pham Van Ngot ◽  
Dang Thi Ngoc Thanh
Keyword(s):  
Folk Medicine ◽  
Agar Plate ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Microscopic Anatomy ◽  
E Coli ◽  
Root Extracts ◽  
Stem Leaf ◽  
Ludwigia Adscendens

The study aimed to supplement data on a plant that was considered a folk medicine of Vietnam. Morphological and microscopic anatomical characteristics of this species which were adapted to the frequently flooded habitat of the reserve was studied and analyzed. Alcoholic extracts of each stem, leaf and root part were recovered using a rotary evaporator. The inhibitory ability on bacterial strains including Bacillus cereus, B. subtilis and Escherichia coli was tested through agar plate diffusion method. The result showed that the stem and leaf extracts were effective against all three bacterial strains, while the root extracts had no effect against E. coli.

scholarly journals Evaluation of Antibacterial Properties of Different Leaf Extracts of Hyptis suaveolens (L) Poit

2021 ◽  
pp. 40-44
Author(s):  
B. A. Erinle ◽  
A. O. Ajayi ◽  
O. T. Osuntokun
Keyword(s):  
Antimicrobial Activity ◽  
Folk Medicine ◽  
Antibacterial Properties ◽  
Extraction Process ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Hyptis Suaveolens

Aim: Hyptis suaveolens is well known for its immense medicinal properties and are beneficial as folk medicine. The objective of the study is to investigate the efficacy of the leaves for it antimicrobial activity. Material and Methods: The leaves of Hyptis suaveolens was  subjected to extraction process using n-hexane, ethanol and distilled water as solvents and the antimicrobial activity was analyzed against different bacterial strains viz.; Escherichia coli, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Salmonella typhi, Enterococcus faecalis by agar well diffusion method. Results: Aqueous extracts showed inhibitory effect against the different tested bacteria organisms with variable zone of inhibitory range 6 -21 mm. Conclusion: The aqueous extracts of Hyptis suaveolens exhibited the presence of highly effective bio-active compounds in these extracts. These can further be evaluated and characterize to improve upon what has been done to create a novel compound that can be useful for various medicinal purposes.

scholarly journals Invitro antibacterial activity of Cassia fistula Linn methanolic leaf extracts

2019 ◽  
Vol 8 (2) ◽  
pp. 270
Author(s):  
Bindhu R. Kamath ◽  
Sabeena Kizhedath
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cassia Fistula ◽  
Leaf Extracts ◽  
E Coli

Background: Cassia fistula Linn is a plant which is widely grown in India and is used for medicinal purposes. The study was carried out with an objective to demonstrate the antimicrobial activity of leaves of Cassia fistula Linn. The aim of the study is to assess antibacterial and antifungal activity of methanolic leaf extract of Cassia fistula Linn against selected clinical isolates.Methods: The antimicrobial activity of methanolic extract of Cassia fistula was evaluated using agar well diffusion method and to zone of inhibition of extract was determined. Clinical isolates of Staphyloccocus aureus, MRSA, Pseudomonas aeruginosa, E. coli and Proteus were screened.Results: The methanolic extracts exhibited antibacterial activity against Staphylococcus aureus. The extract was not active against E. coli, Proteus, MRSA, Pseudomonas aeruginosa. The extract also failed to demonstrate antifungal activity against Candida albicans and Aspergillus niger.Conclusions: The global emergence of multidrug resistant bacterial strains is increasing, limiting the effectiveness of current drugs and treatment failure of infections. A novel approach to the prevention of antibiotic resistance of pathogenic species is the use of new compounds that are not based on existing synthetic antimicrobial agents.

scholarly journals Investigation of the Phyto-constituents and the Antibacterial Activity of Burkea africana against P. aeruginosa, E. coli, S. aureus and E. faecalis

2021 ◽  
pp. 33-40
Author(s):  
S. Zengeni ◽  
J. Chifamba ◽  
I. Mutingwende ◽  
T. Manyarara
Keyword(s):  
Antibacterial Activity ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Antibacterial Efficacy ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
E Coli

Bacterial skin infections are common in the tropics and the emergence of resistant bacterial strains has complicated treatment outcomes especially in immune compromised patients. This study focused on determining the antibacterial activity and preliminary phytochemical screening of the plant, Burkea Africana, which has been used for decades to treat various ailments in Southern Africa. Phyto-constituent determination and antibacterial assay was carried out on the hydro-ethanolic and aqueous leaf extracts of Burkea Africana. The Agar well diffusion method was used to investigate the antibacterial efficacy of both extracts. Activity was measured against P. aeruginosa, E. coli, S. aureus and E. faecalis using ciprofloxacin as the standard. For both extracts, phytochemical analysis revealed the presence of steroids, tannins, saponins and carbohydrates. The 70% hydro-ethanolic extract however revealed more phyto-constituents including flavonoids and alkaloids. For both extracts antibacterial activity was comparable to the standard. The highest zone of inhibition recorded for the water extract was 22±0.4 mm at 500 mg/ml for E. coli while the standard, Ciprofloxacin had a zone of inhibition of 25±0.33 mm. The ethanolic extract showed higher antibacterial efficacy when compared to ciprofloxacin against S. aureus. The zone of inhibition for the 70% ethanolic extract was 25.4±0.50 mm and that of the standard was 27±0.48 mm. Antibacterial activity was generally higher in the ethanolic extract than the water extract. It is therefore concluded that B. africana exhibits antibacterial activity.

scholarly journals STUDY OF VARIOUS FIVE EXTRACTS OF TRACHYSPERMUM AMMI SEEDS ON SELECTED FOUR GRAM-NEGATIVE COOKED FOOD SPOILAGE BACTERIAL STRAINS BY DISK DIFFUSION METHOD

2015 ◽  
Vol 3 (9SE) ◽  
pp. 1-3
Author(s):  
Usha Masih
Keyword(s):  
Antimicrobial Activity ◽  
Cold Water ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
E Coli ◽  
Trachyspermum Ammi

Bacterial foodborne diseases are caused by consumption of foods contaminated with bacteria or their toxins. This study evaluated antibacterial properties of Trachyspermum ammi Acetone, ethanol, methanol, cold water and hard water extracts of spice (Ajowan) seeds against four gram negative strains of pathogenic foodborne bacteria, E. coli O157:H7   ATCC 43888, ATCC 25922, ATCC 8739 and ATCC 43895 that cause infection and intoxication. E. coli ATCC 43888 was observed to be highly susceptible to all extracts of ajowan with absolute zones of inhibition in the range of 16mm – 23mm in diameter. This study demonstrated that spice extracts have antimicrobial activity against food-borne bacterial species and should be considered as potential antibacterial agents for addition to ready meals. The spice contain high amount of secondary metabolites due to these metabolites they have high antimicrobial activity and it can be used as good bio- preserver and it can also use for medicinal purpose.

scholarly journals ANTIMICROBIAL ACTIVITY OF SOME FOLK MEDICINAL PLANTS USED IN RAJASTHAN AGAINST SELECTED PATHOGENIC MICROORGANISMS

2017 ◽  
Vol 9 (3) ◽  
pp. 84
Author(s):  
Maya Verma ◽  
Ashwani Kumar
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Phyllanthus Niruri ◽  
E Coli ◽  
Methanol Extracts ◽  
Highly Active

Objective: To investigate the antibacterial efficacy of methanol extracts of leaves and roots of B. diffusa, Eclipta alba, Phyllanthus niruri and Ricinus communis.Methods: The antimicrobial efficacy of methanol extracts of some medicinal plants was evaluated by agar well diffusion method against selected pathogenic bacterial strains. Gram+ve strains (S. aureus, B. subtilis) were tested and Gram-ve strains tested were (E. coli, S. typhii and K. pneumoniae). Antifungal activity against was tested.Results: B. diffusa and P. niruri leaf extract showed highest antibacterial activity against S. aureus and S. typhii. Leaf extract of P. niruri and R. communis showed highest antifungal activity against A. niger and C. albicans respectivelyConclusion: The methanolic leaf extracts of B. diffusa and P. niruri were highly active against S. aureus and S. typhii.

scholarly journals ANTIMICROBIAL AND ANTIOXIDANT EFFECT OF NATURAL EXTRACTS FROM LEAVES, ROOT, STEM AND FLOWERS OF BACCHARIS LATIFOLIA FROM ECUADOR

2020 ◽  
pp. 78-84
Author(s):  
FAVIAN BAYAS-MOREJON ◽  
ANGELICA TIGRE ◽  
RIVELINO RAMON ◽  
DANILO YANEZ
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Potassium Phosphate ◽  
Natural Antioxidants ◽  
Butylated Hydroxytoluene ◽  
Penicillin G ◽  
Diffusion Method ◽  
E Coli ◽  
Antibacterial And Antioxidant Activity

Objective: The increase in chronic and degenerative diseases and the use of synthetic antioxidants such as (butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT)) are being restricted because they can be considered carcinogenic. Therefore, there is a growing interest in the search for natural antioxidants, especially from plants, due to their content in different bioactive compounds, such as antioxidants and antimicrobials. To evaluate the antibacterial and antioxidant activity of Baccharislatifolia extracts. Methods: For the determination of the antimicrobial activity of extracts of leaves, root, stem and flowers of Baccharislatifolia (Bl), the disk plate diffusion method was used, the strains of Listeria, Salmonella and E. coli were studied; antibiotics Penicillin G and Ciprofloxacin were the controls. For the antioxidant activity, a solution of H2O2 (Abs at 230 nm) was prepared in Potassium Phosphate Monobasic-Sodium Hydroxide buffer. Results: The antimicrobial activity against Listeria and Salmonella, showed that the extracts of leaves and flowers were more effective with inhibition zones>15 mm and>20 mm respectively. In front of E. coli, the extracts of flowers and stem were the best with zones>7.0 mm. Antibiotics studied inhibited the development of Listeria and Salmonella. However, E. coli isolates were resistant. In the antioxidant activity, the flower extract of Bl in 60 mg/ml presents a higher effect with 47.25%. Conclusion: Bl extracts from leaves and flowers were more efficient both in their antimicrobial and antioxidant capacity.

scholarly journals Antibacterial Activity of Leukocyte- and Platelet-Rich Plasma: AnIn VitroStudy

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Agata Cieślik-Bielecka ◽  
Tadeusz Bold ◽  
Grzegorz Ziółkowski ◽  
Marcin Pierchała ◽  
Aleksandra Królikowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Platelet Rich Plasma ◽  
Antibacterial Properties ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Bovine Thrombin ◽  
Zones Of Inhibition

The aim of the study was to investigate the leukocyte- and platelet-rich plasma (L-PRP) antimicrobial activity. The studied sample comprised 20 healthy males. The L-PRP gel, liquid L-PRP, and thrombin samples were testedin vitrofor their antibacterial properties against selected bacterial strains using the Kirby-Bauer disc diffusion method. Two types of thrombin were used (autologous and bovine). Zones of inhibition produced by L-PRP ranged between 6 and 18 mm in diameter. L-PRP inhibited the growth ofStaphylococcus aureus(MRSA and MSSA strains) and was also active againstEnterococcus faecalisandPseudomonas aeruginosa. There was no activity againstEscherichia coliandKlebsiella pneumoniae. The statistically significant increase of L-PRP antimicrobial effect was noted with the use of major volume of thrombin as an activator. Additionally, in groups where a bovine thrombin mixture was added to L-PRP the zones of inhibition concerning MRSA,Enterococcus faecalis, andPseudomonas aeruginosawere larger than in the groups with autologous thrombin. Based on the conducted studies, it can be determined that L-PRP can evokein vitroantimicrobial effects and might be used to treat selected infections in the clinical field. The major volume of thrombin as an activator increases the strength of the L-PRP antimicrobial effect.

scholarly journals The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

2019 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Murni Halim
Keyword(s):  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Natural Drugs ◽  
Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

scholarly journals Decoding Antibacterial and Antibiofilm Properties of Cinnamon and Cardamom Essential Oils: A Combined Molecular Docking and Experimental Study

2021 ◽  
Author(s):  
Elahe Pourkhosravani ◽  
fatemeh dehghan nayeri ◽  
Mitra Mohammadi Bazargani
Keyword(s):  
Cell Attachment ◽  
Antibacterial Properties ◽  
Binding Mode ◽  
Dilution Method ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Biofilm Growth ◽  
Computational Docking ◽  
E Coli ◽  
Industrial Strains

Abstract This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiveness of prepared EOs by two-fold serial dilution method and MTT evaluation. Synergic antibacterial properties of EOs against target strains were studied by using checkerboard titration method. Biofilm growth and development were evaluated using CV and XTT reduction assays. Antibacterial activity was observed for EOs against both bacterial strains with stronger activity for CiEO against both bacteria. The synergistic antibacterial effect was observed only against B. subtilis. Based on the FIC index, combinations could not inhibit the growth of E. coli. The pure EOs and their combination inhibited cell attachment for both studied bacteria with stronger effect on E. coli. CV and XTT reduction assays results showed that Ci EO and its combination with CaEO had the highest antibiofilm activity at lowest MIC value 0.08% and 0.04/0.02% against biofilm formed by E. coli and B. subtilis respectively, indicating a high antibiofilm potential. Computational docking analyses also postulated that the active constituents of evaluated EOs have the potential to interact with different bacterial targets, suggested binding mode of action of EOs metabolites. By and large, synergistic anti-biofilm properties of EOs may provide further options for developing novel formula to inhibit a variety of infectious clinical and industrial strains without (or less) toxicity effects on human body.

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