scholarly journals STUDIES ON OXIDATIVE STRESS MARKERS OF ALLOXAN –INDUCED DIABETIC WISTAR RATS TREATED WITH TETRACARPIDIUM CONOPHORUM AND PIPER GUINEENSE (1:1 MIXTURE).

2018 ◽  
Vol 6 (5) ◽  
pp. 571-579
Author(s):  
ObomanuF G ◽  
Dokubo A
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Oxidative Stress Markers ◽  
Piper Guineense ◽  
Stress Markers

scholarly journals The subchronic effects of 3,4-methylendioxymethamphetamine on oxidative stress in rat brain

2014 ◽  
Vol 66 (3) ◽  
pp. 1075-1081
Author(s):  
Ivan Simic ◽  
Violeta Iric-Cupic ◽  
Rada Vucic ◽  
Marina Petrovic ◽  
Violeta Mladenovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rat Brain ◽  
Wistar Rats ◽  
Superoxide Radical ◽  
Reduced Glutathione ◽  
Oxidative Stress Markers ◽  
Sod Activity ◽  
Stress Markers ◽  
Male Wistar Rats

The aim of the present study was to evaluate the subchronic effects of 3,4-methylenedioxymethamphetamine on several oxidative stress markers: index of lipid peroxidation (ILP), superoxide dismutase (SOD) activity, superoxide radical (O2.-) levels, and reduced glutathione (GSH) levels in the frontal cortex, striatum and hippocampus of the rat. The study included 64 male Wistar rats (200-250g). The animals were treated per os with of 5, 10, or 20 mg/kg of 3,4-methylenedioxymethamphetamine (MDMA) every day for 15 days. The subchronic administration of MDMA resulted in an increase in ILP, SOD and O2.-, and a decrease in GSH, from which we conclude that oxidative stress was induced in rat brain.

scholarly journals African Vegetables (Clerodendrum volibile Leaf and Irvingia gabonensis Seed Extracts) Effectively Mitigate Trastuzumab-Induced Cardiotoxicity in Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Olufunke Olorundare ◽  
Adejuwon Adeneye ◽  
Akinyele Akinsola ◽  
Sunday Soyemi ◽  
Alban Mgbehoma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Cardiac Tissue ◽  
Histopathological Examination ◽  
Radical Scavenging ◽  
Metastatic Breast ◽  
Heart Tissue ◽  
Oxidative Stress Markers ◽  
Her2 Positive ◽  
Stress Markers

Trastuzumab (TZM) is a humanized monoclonal antibody that has been approved for the clinical management of HER2-positive metastatic breast and gastric cancers but its use is limited by its cumulative dose and off-target cardiotoxicity. Unfortunately, till date, there is no approved antidote to this off-target toxicity. Therefore, an acute study was designed at investigating the protective potential and mechanism(s) of CVE and IGE in TZM-induced cardiotoxicity utilizing cardiac enzyme and oxidative stress markers and histopathological endpoints. 400 mg/kg/day CVE and IGE dissolved in 5% DMSO in sterile water were investigated in Wistar rats injected with 2.25 mg/kg/day/i.p. route of TZM for 7 days, using serum cTnI and LDH, complete lipid profile, cardiac tissue oxidative stress markers assays, and histopathological examination of TZM-intoxicated heart tissue. Results showed that 400 mg/kg/day CVE and IGE profoundly attenuated increases in the serum cTnI and LDH levels but caused no significant alterations in the serum lipids and weight gain pattern in the treated rats. CVE and IGE profoundly attenuated alterations in the cardiac tissue oxidative stress markers’ activities while improving TZM-associated cardiac histological lesions. These results suggest that CVE and IGE could be mediating its cardioprotection via antioxidant, free radical scavenging, and antithrombotic mechanisms, thus, highlighting the therapeutic potentials of CVE and IGE in the management of TZM-mediated cardiotoxicity.

scholarly journals Sulphoraphane Supplementation Ameliorates Behavioural Impairments and Hippocampal Neurodegeneration Induced by Lead Exposure in Adult Wistar Rats

2020 ◽  
Vol 11 (2) ◽  
pp. 88-98
Author(s):  
Babatunde Ogunlade ◽  
◽  
Olasumbo Afolayan ◽  
Sunday Adelakun ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Wistar Rats ◽  
Neurodegenerative Disorder ◽  
Antioxidant Properties ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Pb Exposure ◽  
Group B ◽  
Behavioural Tests

Lead (Pb) exposure induces oxidative stress causing imbalance in antioxidant enzymes, cognitive impairments and neurodegeneration. This study investigated the neuroprotective and antioxidant properties of sulphoraphane (SFN) on Pb-induced neurotoxicity of adult Wistar rats. Forty animals (150 ± 20 g) were divided into four groups (n=10): Group A received normal saline as placebo; Group B received 50 mg/kg body weight (bw) of Lead only; Group C received a combination of 50 mg/kg bw of Lead and 50 mg/kg bw of SFN; Group D received 50 mg/kg bw of SFN only. All administration was through oral gavages for 28 days; animals underwent behavioural tests (Morris water and Y- mazes); and thereafter sacrificed and brains extracted. Biochemical estimations of antioxidants (superoxide dismutase, reduced glutathione, and catalase), oxidative stress markers (malondialdehyde, nitric oxide, and hydrogen peroxide), neurotransmitters (dopamine, serotonin, and norepinephrine) and hippocampal histology were done. The results showed significant increase in escape latency, norepinephrine and oxidative stress markers with concomitant decrease percentage correct alternation, serotonin, dopamine and antioxidant enzymes in Pb exposed rats compared with the control. However, the co-administration of SFN and Pb significantly attenuated Pb neurotoxicity. Sulphoraphane is capable of ameliorating oxidative stress induced neurobehavioural deficits and hippocampal neurochemistry caused by Pb exposure in Alzheimer’s type animal model of neurodegenerative disorder.

scholarly journals Chronic Antioxidant Treatment Improves Arterial Renovascular Hypertension and Oxidative Stress Markers in the Kidney in Wistar Rats

2010 ◽  
Vol 23 (5) ◽  
pp. 473-480 ◽  
Author(s):  
E. E. Nishi ◽  
E. B. Oliveira-Sales ◽  
C. T. Bergamaschi ◽  
T. G. C. Oliveira ◽  
M. A. Boim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renovascular Hypertension ◽  
Wistar Rats ◽  
Oxidative Stress Markers ◽  
Antioxidant Treatment ◽  
Stress Markers ◽  
And Oxidative Stress

scholarly journals Irvingia gabonensis Seed Extract: An Effective Attenuator of Doxorubicin-Mediated Cardiotoxicity in Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Olufunke Olorundare ◽  
Adejuwon Adeneye ◽  
Akinyele Akinsola ◽  
Phillip Kolo ◽  
Olalekan Agede ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Secondary Metabolites ◽  
Wistar Rats ◽  
Troponin I ◽  
Disease Risk ◽  
Seed Extract ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Irvingia Gabonensis

Cardiotoxicity as an off-target effect of doxorubicin therapy is a major limiting factor for its clinical use as a choice cytotoxic agent. Seeds of Irvingia gabonensis have been reported to possess both nutritional and medicinal values which include antidiabetic, weight losing, antihyperlipidemic, and antioxidative effects. Protective effects of Irvingia gabonensis ethanol seed extract (IGESE) was investigated in doxorubicin (DOX)-mediated cardiotoxicity induced with single intraperitoneal injection of 15 mg/kg of DOX following the oral pretreatments of Wistar rats with 100-400 mg/kg/day of IGESE for 10 days, using serum cardiac enzyme markers (cardiac troponin I (cTI) and lactate dehydrogenase (LDH)), cardiac tissue oxidative stress markers (catalase (CAT), malonyldialdehyde (MDA), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px), and reduced glutathione (GSH)), and cardiac histopathology endpoints. In addition, both qualitative and quantitative analyses to determine IGESE’s secondary metabolites profile and its in vitro antioxidant activities were also conducted. Results revealed that serum cTnI and LDH were significantly elevated by the DOX treatment. Similarly, activities of tissue SOD, CAT, GST, and GSH levels were profoundly reduced, while GPx activity and MDA levels were profoundly increased by DOX treatment. These biochemical changes were associated with microthrombi formation in the DOX-treated cardiac tissues on histological examination. However, oral pretreatments with 100-400 mg/kg/day of IGESE dissolved in 5% DMSO in distilled water significantly attenuated increases in the serum cTnI and LDH, prevented significant alterations in the serum lipid profile and the tissue activities and levels of oxidative stress markers while improving cardiovascular disease risk indices and DOX-induced histopathological lesions. The in vitro antioxidant studies showed IGESE to have good antioxidant profile and contained 56 major secondary metabolites prominent among which are γ-sitosterol, Phytol, neophytadiene, stigmasterol, vitamin E, hexadecanoic acid and its ethyl ester, Phytyl palmitate, campesterol, lupeol, and squalene. Overall, both the in vitro and in vivo findings indicate that IGESE may be a promising prophylactic cardioprotective agent against DOX-induced cardiotoxicity, at least in part mediated via IGESE’s antioxidant and free radical scavenging and antithrombotic mechanisms.

scholarly journals Effect of Photobiomodulation Therapy on Oxidative Stress Markers in Healing Dynamics of Diabetic Neuropathic Wounds in Wistar Rats

2021 ◽  
Author(s):  
Gagana Karkada ◽  
G. Arun Maiya ◽  
Praveen Arany ◽  
Mohandas Rao ◽  
Shalini Adiga ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Photobiomodulation Therapy ◽  
Control Group ◽  
Sciatic Nerve Crush ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Experimental Group

Abstract Background Prolonged and overlapping phases of wound healing in diabetes are mainly due to the redox imbalance resulting in the chronicity of the wound. Photobiomodulation therapy works on the principle of absorption of photon energy and its transduction into a biological response in the living tissue. It alleviates the cellular responses, thereby improving the mechanism of wound healing in diabetes. Objective To find out the effect of photobiomodulation therapy of dosage 4 J/cm2 in the healing dynamics of diabetic neuropathic wounds in Wistar rats and its relation with oxidative stress markers. Methodology Diabetes was induced using Streptozotocin of 60 mg/kg of body weight to eighteen female Wistar rats. Neuropathy was induced by the sciatic nerve crush injury followed by an excisional wound of 2 cm2 on the back of the animal. Experimental group animals were treated with dosage 4 J/cm2 of wavelength 655 and 808 nm, and control group animals were kept unirradiated. The biomechanical, histopathological, and biochemical changes were analysed in both groups. Results There was a reduction in mean wound healing time and an increased rate of wound contraction in the experimental group animals compared to its control group. The experimental group showed improved redox status, and histopathological findings revealed better proliferative cells, keratinisation, and epithelialization than un-irradiated controls. Conclusions Photobiomodulation therapy of dosage 4 J/cm2 enhanced the overall wound healing dynamics of the diabetes-induced neuropathic wound and optimised the oxidative status of the wound, thereby facilitating a faster healing process.

scholarly journals Effect of sitagliptin on depression in male wistar rats

2019 ◽  
Vol 9 (1) ◽  
pp. 200
Author(s):  
Vidya M. Mahalmani ◽  
Anil P. Hogade ◽  
Sanjay K. Mishra
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Wistar Rats ◽  
Cytokine Gene Expression ◽  
Antidepressant Effect ◽  
Oxidative Stress Markers ◽  
Cytokine Gene ◽  
Stress Markers ◽  
Group I ◽  
Male Wistar Rats

Background: Growing evidence supports relationship between depression and inflammation. The hypothesis of involvement of inflammatory pathways in depression is supported by the findings of increased levels of proinflammatory cytokines. So, we decided to evaluate the effect of sitagliptin on depression using forced swim test (FST) and possible effects of sitagliptin on serum oxidative stress markers and cytokine gene expression in rat hippocampus.Methods: FST model was used to evaluate antidepressant effect in male wistar rats. Rats in group I (control group) were given normal saline, group II (standard group) were given fluoxetine, group III and IV (test groups) were given sitagliptin 5 mg/kg and sitagliptin 9 mg/kg respectively. All the drugs in all groups were given per orally. At the end, animals were sacrificed and blood was collected. Hippocampus of rat brain was dissected out. Serum oxidative stress markers and hippocampal pro inflammatory cytokine gene expression analysis was carried out.Results: Sitagliptin 5 mg/kg and 9 mg/kg showed reduction in depressive symptoms and hippocampal cytokine gene expression in comparison to control. In case of serum oxidative stress markers, there was statistically significant reduction in nitric oxide levels with stagliptin 9 mg/kg. Although there was a decrease in the levels of catalase and increase in the levels of glutathione with standard and test groups, the results were not statistically significant.Conclusions: The present study showed significant antidepressant effect activity of standard and test groups. Hence, further research should be carried out to substantiate above results.

scholarly journals Hepatoprotective Activities of Ethanolic Roots Extract of Ageratum Conyzoides on Alloxan-Induced Hepatic Damage in Diabetic Wistar Rats

2018 ◽  
Vol 36 (01) ◽  
pp. 039-045 ◽  
Author(s):  
A.O. Ojewale ◽  
H.B. Akpan ◽  
F.A. Faduyile ◽  
P.D. Shallie ◽  
A.A. Akande ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Wistar Rats ◽  
Diabetic Rats ◽  
Hepatic Damage ◽  
Oxidative Stress Markers ◽  
Ageratum Conyzoides ◽  
Stress Markers

Introduction The aim of the present study was to evaluate the hepatoprotective activities of the ethanolic roots extract of Ageratum conyzoides (AC) in alloxan-induced hepatic damage in diabetic rats. Materials and Methods Diabetes was induced in Wistar rats by the administration of alloxan (150 mg/kg, intraperitoneal). The ethanolic roots extract of AC, at doses of 250 and 500 mg/kg of body weight, was administered to diabetes-induced rats at a single dose per day for a period of 28 days. Results The effect of the ethanolic roots extract of AC on blood glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and hepatic oxidative stress markers was measured in the diabetic rats. The ethanolic roots extract of AC exhibited significant reduction of blood glucose (p < 0.05) at the dose of 500 mg/kg when compared with the standard drug glibenclamide (600 µg/kg). The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels increased significantly (p < 0.05) in the diabetic group without treatment when compared with the control group. In addition, the levels of oxidative stress markers, such as superoxide dismutase (SOD), catalase (CT), glutathione peroxidase (GPx), and glutathione (GSH), were significantly decreased in the diabetic rats compared with the normal rats, while the lipid peroxidation significantly increased in the diabetic group without treatment compared with the control (normal) group. The results demonstrated that the morphological, functional and oxidative stress changes in the liver caused by the ingestion of alloxan were attenuated in diabetic rats treated with the ethanolic roots extract of AC. Conclusion We concluded that the ethanolic roots extract of AC possesses significant antidiabetic, antioxidant and hepatoprotective effects on alloxan-induced diabetic rats.

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